LOCUS       AAV-GfaABC1D-MCS--4x6T  5474 bp ds-DNA     circular     09-DEC-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..629
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     misc_feature    673..802
                     /label="AAV2 ITR "
                     /ApEinfo_revcolor="#00c72e"
                     /ApEinfo_fwdcolor="#00c72e"
     misc_feature    817..1496
                     /label="GfaABC1D"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1553..1572
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    1553..2081
                     /label="4x6T miR cassette"
                     /ApEinfo_revcolor="#0045c6"
                     /ApEinfo_fwdcolor="#0045c6"
     misc_feature    1573..1595
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    1596..1617
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1618..1639
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    1640..1661
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    1662..1683
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    1684..1705
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    1706..1725
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    1726..1748
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    1749..1770
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1771..1792
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    1793..1814
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    1815..1834
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    1835..1857
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    1858..1879
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1880..1901
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    1902..1923
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    1929..1950
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    1951..1972
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    1973..1992
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    1993..2015
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    2016..2037
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2038..2059
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    2060..2081
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    2233..2829
                     /label="WPRE"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(2709..2720)
                     /label="FactorXa site"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     polyA_signal    2883..3363
                     /label="hGH polyA signal"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3409..3538)
                     /label="AAV2 itr"
                     /ApEinfo_revcolor="#c96b00"
                     /ApEinfo_fwdcolor="#c96b00"
     misc_feature    3409..3550
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#00c636"
                     /ApEinfo_fwdcolor="#00c636"
     Promoter        4396..4424
                     /label="Amp prom"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     CDS             4664..5323
                     /label="AmpR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt
      721 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
      781 aactccatca ctaggggttc ctgcggccgc acgcgtacat atcctggtgt ggagtagggg
      841 acgctgctct gacagaggct cgggggcctg agctggctct gtgagctggg gaggaggcag
      901 acagccaggc cttgtctgca agcagacctg gcagcattgg gctggccgcc ccccagggcc
      961 tcctcttcat gcccagtgaa tgactcacct tggcacagac acaatgttcg gggtgggcac
     1021 agtgcctgct tcccgccgca ccccagcccc cctcaaatgc cttccgagaa gcccattgag
     1081 cagggggctt gcattgcacc ccagcctgac agcctggcat cttgggataa aagcagcaca
     1141 gccccctagg ggctgccctt gctgtgtggc gccaccggcg gtggagaaca aggctctatt
     1201 cagcctgtgc ccaggaaagg ggatcagggg atgcccaggc atggacagtg ggtggcaggg
     1261 ggggagagga gggctgtctg cttcccagaa gtccaaggac acaaatgggt gaggggagag
     1321 ctctccccat agctgggctg cggcccaacc ccaccccctc aggctatgcc agggggtgtt
     1381 gccaggggca cccgggcatc gccagtctag cccactcctt cataaagccc tcgcatccca
     1441 ggagcgagca gagccagagc aggttggaga ggagacgcat cacctccgct gctcgcttaa
     1501 ttaagaattc gctagccaat tgggatccat cgattgatca actagtcata tgggcattca
     1561 ccgcgtgcct tactacgcgt attcttaagc aataagcgaa tataacacgg tcgatctaaa
     1621 aaggttagct gggtgtgtta gaagccctgc aagacgacct gcgcattatt actcacggta
     1681 cgacgcatta ttactcacgg tacgaggcat tcaccgcgtg ccttactacg cgtattctta
     1741 agcaataagc gaatataaca cggtcgatct aaaaaggtta gctgggtgtg ttagaagccc
     1801 tgcaagacga cctgggcatt caccgcgtgc cttactacgc gtattcttaa gcaataagcg
     1861 aatataacac ggtcgatcta aaaaggttag ctgggtgtgt tagaagccct gcaagacgac
     1921 ctgggtaccg cattattact cacggtacga cgcattatta ctcacggtac gaggcattca
     1981 ccgcgtgcct tactacgcgt attcttaagc aataagcgaa tataacacgg tcgatctaaa
     2041 aaggttagct gggtgtgtta gaagccctgc aagacgacct gggtaccagg taaagcggcc
     2101 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     2161 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattctgc ctcgagcagc
     2221 gatatcatac cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2281 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2341 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2401 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2461 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2521 tcgctttccc cctccctatt gccacggtgg aactcatcgc cgcctgtctt gcccgctgct
     2581 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     2641 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     2701 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2761 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     2821 ccccgcctgt ctagagtcga cctgcagaag cttgcctcga gcagcgctgc tcgagagatc
     2881 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     2941 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     3001 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     3061 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     3121 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     3181 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     3241 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     3301 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     3361 ctgattttgt aggtaaccac gtggtggtgg tgcggaccga gcggccgcag gaacccctag
     3421 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     3481 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     3541 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3601 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3661 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
     3721 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3781 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     3841 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3901 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
     3961 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
     4021 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     4081 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     4141 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4201 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4261 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4321 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4381 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4441 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4501 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4561 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4621 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4681 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4741 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4801 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4861 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4921 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4981 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5041 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5101 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5161 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5221 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5281 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5341 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5401 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5461 agcgtcagac cccg
//