LOCUS       AncNeo+sub7             9101 bp    DNA     circular     05-JAN-2023
DEFINITION  .
ACCESSION   urn.local...3ya-fr5g2v4
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     rep_origin      102..557
     CDS             689..1549
                     /standard_name="Ampicillin"
     rep_origin      1694..2367
     misc_feature    2611..2835
                     /standard_name="Tn7R"
     CDS             complement(2902..3435)
                     /standard_name="Gentamicin"
     misc_feature    complement(3992..4274)
                     /note="Geneious type: polyA_signal"
                     /standard_name="HSV\tk\pA"
     CDS             complement(4274..5201)
                     /standard_name="Anc_neo"
     regulatory      complement(5210..5331)
                     /note="Geneious type: promoter"
                     /standard_name="p10\prom"
     regulatory      5350..5478
                     /note="Geneious type: promoter"
                     /standard_name="pPH\prom"
     CDS             5478..8567
                     /standard_name="Anc_Neoaves_ins1_S7"
     misc_feature    8585..8825
                     /note="Geneious type: polyA_signal"
                     /standard_name="SV40\pA"
     misc_feature    8854..9019
                     /standard_name="Tn7L"
ORIGIN      
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4261 tcccccatct cccggtacct taggaggact tgatatcgaa cttgatgtcg aaacggccct
     4321 ggaaacggtc cttgtcggag tactggatgt tctggccgta agcacggcac tcgacacgca
     4381 cttccatgtc gtaggtcagg ttggtgaact gcacagccac cagaggttgc aggtaacggg
     4441 gctgcagcag ctttccgtag taagggtagt actgcagagc gaaaccaggg taaccgccca
     4501 tgccgtagta ctcgacggtg ccgatcttgt cagcgtcctc ttcacgctta gcggtgcagt
     4561 gcacggggat caagtagggg ttgtacttag ccatcacttc aggaggcagg gactcgttca
     4621 cgggagcctt gggcttgaaa ccgatgatac ggttcagctt gaccaggatg caaggcttgc
     4681 cgtcacggta accgaaggtg gggtcgttga taccggagca gttctcgagc cactcgcgct
     4741 tgaacttgca gactttcttc tggccctgag cgtcgttgta gggaccacgc tctttgtagt
     4801 cggtggggat gtcgccgcag tcctggaaga tgatgttctc ggtctgctgg tcagcggagt
     4861 agtccttcag gaacttctcg aggttcttca cgtagggctc gtaggacttg gggtcagaag
     4921 cggtgaagga gatctcggtc ttttgcacct gaggcacttg ggtcagtcca ggaggagcca
     4981 cacggtcttg gtacttgggc tcgaactcgg acacggtcag cagcatcact tggatggtgc
     5041 cgatgaagat accagccagg caaccgtaga agatcacgta gaacagcagg atcttgaacc
     5101 aggaaccacc ggtacgaccc agcagctctt tcttctcgga gttccagatg aacttcttcc
     5161 agttgccgtc gccgtcttta gccttgccac gagccatgat caagtcttcg tcgagtgatt
     5221 gtaaataaaa tgtaatttac agtatagtat tttaattaat atacaaatga tttgataata
     5281 attcttattt aactataata tattgtgttg ggttgaatta aaggtccgta tactccggaa
     5341 tattaataga tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt
     5401 actgttttcg taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc
     5461 gtcccaccat cgggcgcgga tccatgggca aaggtgctgg tcgtgataag tacgagccca
     5521 ccgctacctc tgagcacggt gctaagaaga agaaaaaggg caagaaagaa cgcgagcgcg
     5581 acatggacga gctgaagaaa gaagtctcca tggacgacca caagctgtcc ctcgacgagc
     5641 tgcaccgtaa atacggcacc gacctgtctc gtggactgac ctctgctcgt gctgctgaga
     5701 tcttggctcg tgacggtccc aacactctga cccctccacc tactactccc gagtgggtca
     5761 agttctgccg tcagctgttc ggtggtttct ccctgctgct gtggatcggt gctatcctgt
     5821 gcttcctggc ttacggtatc gagactctgg tcaacggcga gggcaacaac gacaacctgt
     5881 acctgggtgt cgtgctggct gctgtcgtga ttattaccgg ttgcttctcc tactaccaag
     5941 aggccaagtc ctccaagatc atggaatcct tcaagaacat ggtgccccag caggctctgg
     6001 tcgtgcgtaa cggcgagaag atgtccatca acgctgaggg tgtcgtcgtg ggcgacctgg
     6061 ttgaagtgaa aggtggcgac cgcatccccg ctgacctgcg tatcatttcc gctcacggtt
     6121 gcaaggtgga caactcctct ctgaccggcg aatccgagcc tcagactcgt tctcccgact
     6181 tctccaacga gaaccctctg gaaacccgca acattgcttt cttctccacc aactgcgtcg
     6241 agggcaccgc taggggtatc gtgatctcta ctggcgaccg taccgtgatg ggtcgtatcg
     6301 cttctctggc ttccggcctg gaaggtggca agacccctat cgctatggaa atcgagcact
     6361 tcatccacct gattaccggt gtcgctgtgt tcctgggcgt gtccttcttc atcctgtctc
     6421 tgatcctcga gtacacctgg ctcgaggctg tgatcttcct gatcggtatc atcgtggcta
     6481 acgtgcccga gggactgctg gctactgtga ctgtgtgcct gactctgacc gctaagcgta
     6541 tggctcgcaa gaactgcctg gtcaagaacc tggaagctgt ggaaaccctg ggttccacct
     6601 ccaccatctg ctccgacaag accggtacac tgacccagaa ccgtatgacc gtggctcaca
     6661 tgtggttcga caaccagatc cacgaggctg acaccaccga gaaccagtcc ggtgcttcct
     6721 tcgacaagtc ctctgctacc tggaccgctt tgtcccgtgt ggctggactg tgtaaccgtg
     6781 ccgtgttcca ggctaatcaa gagaacgtgc ccatcctgaa gcgtgctgtt gctggcgacg
     6841 cttccgaatc cgctctgctg aagtgcatcg agctgtgctg cggttccgtg aaagagatgc
     6901 gcgagcgtta ccccaaggtg gtcgagatcc ctttcaacag caccaacaag taccagctgt
     6961 ccatccacaa gaacgctaac ccctccgagt ctcgttacct gctggtcatg aagggtgctc
     7021 ccgagcgtat cctggaccgt tgctcctcta tcctgatcca cggcaaagag cagcccctgg
     7081 acgaggaaat gaaggacgct ttccagaacg cttacctgga actcggtggc ctgggagaac
     7141 gtgtgttggg attctgccac ctcgctctgc ccgacgatca gttccctgag ggattccagt
     7201 tcgacaccga cgaagtgaac ttccccgtgg acaagctgtg cttcgtgggc ctgatgtcca
     7261 tgatcgaccc tccaagagct gctgtgccag acgctgttgg aaagtgccgt tccgctggta
     7321 tcaaagtgat catggtcacc ggcgatcacc ccatcaccgc taaggctatc gctaaaggcg
     7381 tgggtatcat ctccgagggc aacgagactg tcgaggatat cgctgctcgt ctgaacatcc
     7441 ctgtctctca agtgaaccct cgtgacgcta aggcctgcgt ggtgcacggt tctgacctga
     7501 aggacatgac ctccgagcag ctggacgaca tcctcatcca ccacaccgag atcgtgttcg
     7561 ctcgtacatc ccctcagcaa aagctgatca tcgtcgaagg ttgccagcgt cagggtgcta
     7621 tcgtggctgt tacaggcgac ggtgtcaacg actcccctgc tctgaaaaag gctgacatcg
     7681 gagtggctat gggtatcgct ggttccgacg tgtccaagca ggctgctgac atgatcctgc
     7741 tcgacgacaa cttcgcttcc atcgtgaccg gcgtggaaga gggtcgtctg atcttcgaca
     7801 acttgaagaa gtcgatcgct tacaccctga cctctaacat ccccgagatc acccctttct
     7861 tgatcttcat cattgcgaac atcccactgc ctctgggcac cgtgaccatc ctgtgcatcg
     7921 acctgggtac tgacatggtc cccgctatct ccctcgctta cgagcaggct gagtccgaca
     7981 tcatgaagcg ccagcctcgt aaccccaaga ccgacaagct ggtcaacgag aggctgatct
     8041 ccatggccta cggccagatc ggcatgatcc aagctctcgg tggcttcttc acctacttcg
     8101 tcatcatggc tgagaacggt ttctggccct ccggcctgtt gggtatcaga gtgcaatggg
     8161 acgaccgttg gatcaacgac gtcgaggact cctacggaca gcagtggact tacgaacagc
     8221 gcaagatcgt cgagttcact tgccacaccg ctttcttcgt gtccatcgtg gtggtgcagt
     8281 gggctgacct gatcatctgc aagacccgtc gtaactctgt gttccagcag ggcatgaaga
     8341 acaagatcct catcttcggc ctgttcgagg aaaccgctct ggctgctttc ctgtcttact
     8401 gccccggcat ggatgtggct ctgcgtatgt accctctgaa gcccacctgg tggttctgcg
     8461 ctttccccta ctctctgctc atcttcctgt acgacgagat ccgcaagctc atcatccgtc
     8521 gcaaccctgg tggctgggtc gagaaagaga cttactacta aaagcttgtc gagaagtact
     8581 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     8641 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     8701 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8761 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8821 ggatctgatc actgcttgag cctaggagat ccgaaccaga taagtgaaat ctagttccaa
     8881 actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta
     8941 ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca
     9001 actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta atcaaacatc
     9061 ctgccaactc catgtgacaa accgtcatct tcggctactt t
//
