LOCUS       Grosbeak                9089 bp    DNA     circular     05-JAN-2023
DEFINITION  .
ACCESSION   urn.local...3xs-fr5ft2u
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     rep_origin      102..557
     CDS             689..1549
                     /standard_name="Ampicillin"
     rep_origin      1694..2367
     misc_feature    2611..2835
                     /standard_name="Tn7R"
     CDS             complement(2902..3435)
                     /standard_name="Gentamicin"
     misc_feature    complement(3992..4274)
                     /note="Geneious type: polyA_signal"
                     /standard_name="HSV\tk\pA"
     CDS             complement(4274..5201)
                     /standard_name="Grosbeak"
     regulatory      complement(5210..5331)
                     /note="Geneious type: promoter"
                     /standard_name="p10\prom"
     regulatory      5350..5478
                     /note="Geneious type: promoter"
                     /standard_name="pPH\prom"
     CDS             5478..8555
                     /note="CI:V1=BamHI,V2=HindIII,I1=BamHI,I2=HindIII"
     misc_feature    8573..8813
                     /note="Geneious type: polyA_signal"
                     /standard_name="SV40\pA"
     misc_feature    8842..9007
                     /standard_name="Tn7L"
ORIGIN      
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4261 tcccccatct cccggtacct taggaggact tgatatcgaa cttgatgtcg aagcggccct
     4321 ggaaacggtc cttgtcagag tagatgatgt tctggccgta agcacggcac tcgacacgca
     4381 cttccacgtc gtaggtcagg ttggtgaact gcacagccac cagaggttgc aggtaacggg
     4441 gctgcagcag ctttccgtag taggggtagt actgcaggcc gaaaccaggg taaccgccca
     4501 tgccgtagta ctcgacggtg ccgatcttgt cagcgtcctc ttcacgcttg gccacgcagt
     4561 gcacggggat caagttgggg ttgtacttag ccatcacttc tggaggcagg gactcgttga
     4621 tgggagcctc gggcttgaaa ccgatgatac ggttcagctt gaccaggatg caaggcttgc
     4681 cgtccttgta accgtaggtg gggtcgttga taccggagca gttctcgagc cactcgcgct
     4741 tgaacttgca gactttcttc tggccctgag cgtcgttgta gggaccacga cctttgtact
     4801 cggtaggggt gtcgccgcag tcctcgaaca cgatgttctc gatctgctgc tcgtcggagt
     4861 agtcgcgcag gaagttgtcc aggttacgca cgtagggctc gtaggaacga gggtcagaag
     4921 cggtgaaagc gatctcggtc ttttgcacct gaggcacttg ggtcagtcca ggaggagcca
     4981 cacggtcttg gtacttgggc tcgaactcgg acacggtcag cagcatcact tggatggtgc
     5041 cgatgaagat accagccagg caaccgtaga agatcacgta gaacagcagg atcttgaacc
     5101 aggaaccacc ggtacgaccc agcagctctt tcttctcgga gttccagatg aacttcttcc
     5161 agttgccgtc gccgtcttta gccttgccac gagccatgat caagtcttcg tcgagtgatt
     5221 gtaaataaaa tgtaatttac agtatagtat tttaattaat atacaaatga tttgataata
     5281 attcttattt aactataata tattgtgttg ggttgaatta aaggtccgta tactccggaa
     5341 tattaataga tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt
     5401 actgttttcg taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc
     5461 gtcccaccat cgggcgcgga tccatgggca aaggtgctgg tcgtgataag tacgagccca
     5521 ccgctacctc tgagcacggt ggaaagaaga aaggcaagaa agaacgcgac atggacgagc
     5581 tgaagaaaga ggtggtcatg gacgaccaca agctgtccct ggacgaactg caccgtaaat
     5641 acggcaccga cctgaaccgt ggactgacct ctgctcgtgc tgctgagatc ttggctcgtg
     5701 acggtcccaa cgctctgacc cctcctccta ctactcccga atggatcaag ttctgccgtc
     5761 agctgttcgg tggtttctcc ctgctgctgt ggatcggtgc tatcctgtgc ttcctggctt
     5821 acgctatcga gactctggtc aacggcgagg gcaacaacga caacctgtac ctgggtatcg
     5881 tgctggctgc tgtcgtgatc gtgaccggtt gcttctctta ctaccaagag gctaagtcct
     5941 ccaagatcat ggaatccttc aagaacctgg tgcctcagca ggctctggtc gtgcgtaacg
     6001 gcgagaagat gtccatcaac gctgagggtg tcgtcgtggg cgacctggtt gaagtgaaag
     6061 gtggcgaccg catccccgct gacctgcgta tcatttccgc tcacggttgc aaggtggaca
     6121 actcctctct gaccggcgaa tccgagcctc agactcgttc tcctgacttc tcccacgaga
     6181 accctctgga aacccgtaac attgctttct tctccaccaa ctgcgtcgag ggtactgctc
     6241 acggtatcgt gatctccacc ggcgaccgta ctgtgatggg tcgtatcgct tccctggctt
     6301 ccggactgga aggtggcaag acccctatcg ctatggaaat cgagcacttc atccacatca
     6361 ttaccggtgt cgctgtgttc ctgggcgtgt ccttcttcat cctgtctctg atcctcgagt
     6421 acacctggct cgaggctgtg atcttcctga tcggtatcat cgtggctaac gtgcccgagg
     6481 gactgctggc tactgtgact gtgtgcctga ctctgaccgc taagcgtatg gctcgcaaga
     6541 actgcctggt caagaacttg gaggctgtgg aaaccctggg ttccacctcc accatctgct
     6601 ccgacaagac cggtactctg acccagaacc gtatgaccgt ggctcacatg tggttcgaca
     6661 accagatcca cgaggctgac accaccgaga accagtccgg tgcttccttc gacaagtcct
     6721 ccgctacctg gaccgctctg tcacgtatcg ctggactgtg caacagggct gttttccagg
     6781 ctggccaaga gaacgtgccc atcctgaaaa gagctgtggc tggcgacgct tccgagtccg
     6841 ctttgctgaa gtgcatcgag ctgtgctgcg gttccgtgaa gcagatgcgt gaacgttacc
     6901 ccaaggtggt cgagatcccc ttcaactcta ccaacaagta ccagctgtcc atccacaaga
     6961 acgctaaccc ctccgagtct cgttacctgc tggtcatgaa gggtgctccc gagcgtatcc
     7021 tggaccgttg ctctaccatc ctgatccacg gcaaagagca gcctctggac gaggaaatga
     7081 aggacgcttt ccagaacgct tacctggaac tcggtggcct gggagaacgt gtgctgggat
     7141 tctgctacct ggctctgccc gacgatcagt tccctgaggg attccagttc gacaccgacg
     7201 acctcaactt ccccgtggac aagctgtgct tcgtgggcct gatgtccatg atcgaccctc
     7261 caagagctgc tgtgccagac gctgttggaa agtgccgttc cgctggtatc aaagtgatca
     7321 tggtcaccgg cgatcacccc atcaccgcta aggctatcgc taaaggcgtg ggtatcatct
     7381 ccgagggaaa cgagactgtc gaggatatcg ctgctcgtct gaacatcccc gtgtctcaag
     7441 tgaaccctcg tgacgctaag gcctgcgtgg tgcacggttc tgacctgaag gacatgacct
     7501 ccgagcagct ggacgacatc ctgctgcacc acaccgagat cgtgttcgct cgtacatccc
     7561 ctcagcaaaa gctgatcatc gtcgagggct gccagcgtca gggtgctatc gttgctgtta
     7621 ctggcgacgg tgtcaacgac tcccctgctc tgaaaaaggc tgacatcgga gtggctatgg
     7681 gaatcgctgg ttccgacgtg tccaagcagg ctgctgacat gatcctgctc gacgacaact
     7741 tcgcttccat cgtgactggt gtcgaggaag gtcgtctgat cttcgacaac ttgaagaagt
     7801 cgatcgctta caccctgacc agcaacatcc ctgagatcac ccctttcttg atcttcatca
     7861 ttgcgaacat ccctctgcct ctgggcaccg tgaccatcct gtgcatcgac ctgggtactg
     7921 acatggtgcc cgctatctcc ctcgcttacg agcaggctga gtccgacatc atgaagcgcc
     7981 agcctcgtaa ccccaagacc gacaagctgg ttaacgagag gctgatctcc atggcttacg
     8041 gccagatcgg catgatccag gctctcggtg gcttcttcac ctacttcgtc atcatggctg
     8101 agaacggttt ctggccctct ggcctgttgg gactgagagt gcaatgggac gaccgttgga
     8161 tcaacgacgt cgaggactcc tacggacagc agtggactta cgaacagcgc aagatcgtcg
     8221 agttcacttg ccacaccgct ttcttcgtgt ccatcgtggt ggtgcagtgg gctgacctga
     8281 tcatctgcaa gacccgtcgt aactccgtgt tccagcaggg catgaagaac aagatcctca
     8341 tcttcggcct gttcgaggaa accgctctgg ctgctttcct gtcttactgc cccggcatgg
     8401 atgtggctct gcgtatgtac cctctgaagc tgacctggtg gttctgcgct ttcccctact
     8461 ctctgttgat cttcgtgtac gatgaagtcc gcaagctcat catccgtcgc aaccctggtg
     8521 gctgggtcga gaaagagact tactactaaa agcttgtcga gaagtactag aggatcataa
     8581 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     8641 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     8701 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8761 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac
     8821 tgcttgagcc taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca
     8881 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     8941 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     9001 gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca
     9061 tgtgacaaac cgtcatcttc ggctacttt
//
