LOCUS       Exported                6794 bp ds-DNA     circular SYN 16-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Mayo Clinic1
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(19..236)
                     /label=ITR(L)
                     /note="Tol2 ITR(L)"
     primer_bind     19..61
                     /label=R15_Tol2-L_F
                     /note="for Jun Ni's project"
     primer_bind     19..48
                     /label=Tol2-mini5_2
     primer_bind     19..38
                     /label=20_Kan_F2
                     /note="9_ A9"
     primer_bind     19..38
                     /label=20_Kan_F
                     /note="9_ A7"
     primer_bind     complement(207..236)
                     /label=Tol2-ITR(L)-O3
     primer_bind     207..226
                     /label=MISC_ITR-L-R2
                     /note="10_E5"
     primer_bind     230..255
                     /label=MISC-Pinch-R1
                     /note="10_E7"
     primer_bind     237..259
                     /label=T7-rc
     primer_bind     complement(237..259)
                     /label=T7
     primer_bind     238..260
                     /label=cmlcBFPpolyA-BamHI_F1
                     /note="8_ I6"
     primer_bind     complement(240..259)
                     /label=T7 Promoter Primer
                     /note="Invitrogen T7 Promoter Primer (Cat. no. N560-02).
                     
                     Invitrogen offers primers for PCR amplification that 
                     complement many of the vectors currently available.  All 
                     primers are: (i) desalted and purified by gel filtration, 
                     (ii) assayed for function by PCR amplification, (iii) 
                     provided in 2microgram quantities.  Please visit 
                     www.invitrogen.com for more information."
     promoter        complement(241..259)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(241..259)
                     /label=T7 promoter2
                     /note="10_D4"
     promoter        join(345..648,650..1173)
                     /label=mCAGS
     primer_bind     complement(345..372)
                     /label=CRISPR-step2-R
                     /note="11_F1"
     primer_bind     complement(345..372)
                     /label=CRISPR-step2-R (1)
                     /note="G4_C2"
     primer_bind     complement(551..565)
                     /label=CRISPR-step2-R
                     /note="11_F1"
     primer_bind     complement(551..565)
                     /label=CRISPR-step2-R (1)
                     /note="G4_C2"
     primer_bind     619..648
                     /label=GIB-SOX9-F1.3
                     /note="Clark_Freezer_Ashley_2_D2"
     primer_bind     891..908
                     /label=Seq_Luc_F2
                     /note="12_B9"
     primer_bind     988..1005
                     /label=Seq_Luc_F1
                     /note="12_B8"
     primer_bind     990..1013
                     /label=PCR-non-codingexon-F
                     /note="11_F4"
     primer_bind     1165..1183
                     /label=SEQ-mIntron-F2
     primer_bind     1169..1186
                     /label=LDLR-SEQ-F1
                     /note="Clark Freezer_Tanya_B45"
     primer_bind     complement(1193..1217)
                     /label=pc-cags PCR R1 (gibson)
                     /note="11_H5"
     primer_bind     1194..1220
                     /label=MISC-pKC-YFC-F1
                     /note="G4_B2"
     primer_bind     1204..1224
                     /label=MiscLp2mc-F1
                     /note="11_A4"
     CDS             1261..1338
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MASVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     1267..1283
                     /label=cox8a max fp
                     /note="Ankit Sabharwal"
     CDS             1351..1758
                     /codon_start=1
                     /label=TALE NTD
                     /translation="VDLRTLGYSQQQQEKIKPKVRSTVAQHHEALVGHGFTHAHIVALS
                     QHPAALGTVAVTYQHIITALPEATHEDIVGVGKQWSGARALEALLTDAGELRGPPLQLD
                     TGQLVKIAKRGGVTAMEAVHASRNALTGAPLN"
     primer_bind     complement(1474..1490)
                     /label=Seq-adaptor
                     /note="11_H1"
     primer_bind     1571..1590
                     /label=TAL_F1
                     /note="For colony PCR and sequencing of Part II TALEN 
                     Golden Gate Assembly"
     primer_bind     1702..1719
                     /label=SEQ-TAL-F2
                     /note="Clark Freezer_Tanya_A71"
     primer_bind     1724..1742
                     /label=SeqTALEN_5-1
                     /note="For sequencing of Part II TALEN Golden Gate 
                     Assembly"
     primer_bind     complement(1751..1762)
                     /label=SV40_SeqF1
                     /note="9_ G2"
     misc_feature    1759..3348
                     /label=ND2 Right TALE
     CDS             3349..3537
                     /codon_start=1
                     /label=TALE CTD
                     /translation="SIVAQLSRPDPALAALTNDHLVALACLGGRPAMDAVKKGLPHAPE
                     LIRRVNRRIGERTSHRVA"
     primer_bind     complement(3398..3417)
                     /label=TAL_R2
                     /note="For colony PCR and sequencing of Part II TALEN 
                     Golden Gate Assembly"
     primer_bind     3398..3417
                     /label=C_TAL_R_BK
                     /note="Bibek Kar_U01"
     primer_bind     3404..3421
                     /label=TAL-F2
                     /note="Clark Freezer_Tanya_A73"
     primer_bind     complement(3459..3475)
                     /label=SEQ-TAL-R3
                     /note="Clark Freezer_Tanya_A72"
     primer_bind     3477..3496
                     /label=GoldyTAL_FokI_1
     primer_bind     complement(3523..3540)
                     /label=MISC-TALE-R1
                     /note="Clark Freezer_Tanya_B24"
     primer_bind     complement(3526..3543)
                     /label=Goldy vec RP
                     /note="Bibek Kar_U01"
     primer_bind     complement(3527..3543)
                     /label=pT3TS vector r
                     /note="Ekker Freezer_Grace_1_B7"
     primer_bind     complement(3531..3543)
                     /label=TAL_R3/TAL_rev3
                     /note="For sequencing of Part II TALEN Golden Gate 
                     Assembly"
     primer_bind     3537..3559
                     /label=1290-1397 ddda-FP
     misc_feature    3538..3543
                     /label=Linker
     CDS             3544..3867
                     /label=DddAtox 1397-N
                     /label=DddA(1290-1397)
                     /note="/created_by=bmok"
                     /note="/modified_by=bmok"
     misc_feature    3571..3612
                     /label=loop, might be involved in dsDNA binding
                     
                     /label=nonstandard type:  polylinker
                     /note="/created_by=bmok"
     misc_feature    3631..3642
                     /label=loop (SPLIT)
                     
                     /label=nonstandard type:  polylinker
                     /note="/created_by=bmok"
     misc_feature    3664..3675
                     /label=loop
                     
                     /label=nonstandard type:  polylinker
                     /note="/created_by=bmok"
     misc_feature    3715..3717
                     /label=E1298A mutant catalytically dead
                     
                     /note="/created_by=bmok"
                     /note="/modified_by=bmok"
     misc_feature    3748..3756
                     /label=loop (SPLIT)
                     /label=nonstandard type:  polylinker
                     /note="/created_by=bmok"
     misc_feature    3823..3834
                     /label=loop (split)
                     
                     /label=nonstandard type:  polylinker
                     /note="/created_by=bmok"
     misc_feature    3850..3867
                     /label=loop
                     
                     /label=nonstandard type:  polylinker
                     /note="/created_by=bmok"
     primer_bind     complement(3859..3879)
                     /label=BamHI-1397Nterm-RP
     misc_feature    3868..3879
                     /label=Linker
     CDS             3880..4128
                     /label=UGI
                     /note="/created_by=kzhao"
     primer_bind     complement(4106..4135)
                     /label=nterm-ugi-RP
     misc_feature    4120..4132
                     /label=user junction
                     
                     /label=nonstandard type:  overhang
                     /note="/created_by=bmok"
     3'UTR           4135..4282
                     /label=ATP5B 3'UTR
                     /note="/created_by=bmok"
                     /note="/modified_by=bmok"
     misc_feature    4136..4282
                     /label=TTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCA
                     /label=TTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCT 
                     GTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCC 
                     TTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCT 
                     GGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCAT 
                     GCTGGGGATGCGGTGGGCTCTATGG
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=TTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTA
                     GTTGC 
                     CAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTC 
                     CCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCA 
                     TTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAAT 
                     AGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG"
     primer_bind     complement(4258..4289)
                     /label=1290-1397 ddda-RP
     terminator      4260..4264
                     /label=AAUAA motif
                     
                     /note="/created_by=bmok"
     polyA_signal    4321..4402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4384..4402)
                     /label=SEQ-SV40(A)R
                     /note="10_E1"
     primer_bind     4424..4443
                     /label=SV40_SeqF1
                     /note="9_ G2"
     polyA_signal    4526..4607
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4589..4607)
                     /label=SEQ-SV40(A)R
                     /note="10_E1"
     primer_bind     4629..4648
                     /label=SV40_SeqF1
                     /note="9_ G2"
     primer_bind     complement(4755..4774)
                     /label=MISC-lacZfix-R1
                     /note="G3_G8"
     primer_bind     complement(4766..4794)
                     /label=pKtol2c_mitotale_apobec1_RP
     rep_origin      complement(4775..4788)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4795..4817)
                     /label=T3-rc
     primer_bind     4795..4817
                     /label=T3
     primer_bind     4796..4815
                     /label=T3 promoter
                     /note="11_G8"
     misc_feature    4821..5011
                     /label=ITR(R)
                     /note="Tol2 ITR(R)"
     primer_bind     4821..4851
                     /label=MISCTol2R-F1
                     /note="8_ G8"
     primer_bind     4896..4923
                     /label=SEQ-ITR_R-O1
                     /note="Sequencing primer for a region of RP2"
     primer_bind     complement(4900..4921)
                     /label=MISC_ITR-R-F2
                     /note="10_E4"
     primer_bind     complement(4948..4967)
                     /label=MISC_ITR-R-F1
                     /note="10_E2"
     primer_bind     complement(4971..5008)
                     /label=R15_Tol2-R_R
                     /note="for Jun Ni's project248|"
     primer_bind     complement(4977..5007)
                     /label=Tol2-mini3_2
     primer_bind     complement(4988..5007)
                     /label=20_Kan_R2
                     /note="9_ B1"
     primer_bind     complement(4988..5007)
                     /label=20_Kan_R
                     /note="9_ A8"
     rep_origin      5025..5906
                     /label=ColE1 Ori
     primer_bind     5025..5044
                     /label=Gib-Kan-Ori-F1
                     /note="Clark Freezer_Ashley_1_G9"
     primer_bind     complement(5038..5054)
                     /label=SEQ-pK-R1
     primer_bind     complement(5092..5118)
                     /label=MISC-ColE1_Ori-R1
                     /note="Sequencing primer for a region of pRP6.1"
     primer_bind     complement(5153..5174)
                     /label=CRISPR-step2-F
                     /note="11_E9"
     primer_bind     complement(5153..5174)
                     /label=CRISPR-step2-F (1)
                     /note="G4_C1"
     primer_bind     5907..5946
                     /label=20_Kan_F2
                     /note="9_ A9"
     primer_bind     5907..5924
                     /label=20_Kan_F
                     /note="9_ A7"
     primer_bind     complement(5918..5938)
                     /label=KanSeq-F
                     /note="Jun Ni's - for sequencing mini-KAN-mini"
     CDS             complement(5939..6748)
                     /codon_start=1
                     /label=Kan(R)
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5972..6001)
                     /label=Kan2 Primer
                     /note="Fragment of KanI Primer; Length: 30"
     primer_bind     complement(5977..6000)
                     /label=KanSeq2-F
                     /note="Jun Ni's - for sequencing 20-KAN-20"
     primer_bind     complement(6296..6319)
                     /label=BSMB1 Removal R1
     primer_bind     6296..6319
                     /label=BSMB1 Removal F1
     primer_bind     6694..6723
                     /label=Kan1 Primer
                     /note="Fragment of KanI Primer; Length: 30"
     primer_bind     6708..6731
                     /label=KanSeq2-R
                     /note="Jun Ni's - for sequencing 20-KAN-20"
     primer_bind     6724..6743
                     /label=KanSeq-R
                     /note="Jun Ni's - for sequencing mini-KAN-mini"
     primer_bind     complement(6725..6748)
                     /label=Gib-Kan-Ori-R1
                     /note="Clark Freezer_Ashley_1_H1"
     primer_bind     6758..6783
                     /label=SEQ-pK-F1
     primer_bind     complement(join(6762..6794,1..7))
                     /label=20_Kan_R2
                     /note="9_ B1"
     primer_bind     complement(join(6774..6794,1..7))
                     /label=20_Kan_R
                     /note="9_ A8"
     primer_bind     join(6794,1..18)
                     /label=pIndigoBAC536(HA)-InvTol2_F
                     /note="9_ H4"
ORIGIN
        1 cacgatgcag ctggctccca gaggtgtaaa gtacttgagt aattttactt gattactgta
       61 cttaagtatt atttttgggg atttttactt tacttgagta caattaaaaa tcaatacttt
      121 tacttttact taattacatt tttttagaaa aaaaagtact ttttactcct tacaatttta
      181 tttacagtca aaaagtactt attttttgga gatcacttca ttctattttc ccttggtctc
      241 cctatagtga gtcgtattag atcccgggat tactagttta aacctagtta ttaatagtaa
      301 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      361 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      421 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      481 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      541 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      601 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
      661 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
      721 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc gcgcgccagg
      781 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa
      841 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
      901 taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg
      961 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     1021 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     1081 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagctag agcctctgct
     1141 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     1201 ctgtctcatc attttggcaa agaattttaa gctaggatct aactcgagag atctagcacc
     1261 atggcctccg ttctgacccc gctgctgctg cgtggcctga ccggctccgc tcgtcgtctg
     1321 ccagttccgc gtgcgaaaat ccattccctg gtggatctac gcacgctcgg ctacagtcag
     1381 cagcagcaag agaagatcaa accgaaggtg cgttcgacag tggcgcagca ccacgaggca
     1441 ctggtgggcc atgggtttac acacgcgcac atcgttgcgc tcagccaaca cccggcagcg
     1501 ttagggaccg tcgctgtcac gtatcagcac ataatcacgg cgttgccaga ggcgacacac
     1561 gaagacatcg ttggcgtcgg caaacagtgg tccggcgcac gcgccctgga ggccttgctc
     1621 acggatgcgg gggagttgag aggtccgccg ttacagttgg acacaggcca acttgtgaag
     1681 attgcaaaac gtggcggcgt gaccgcaatg gaggcagtgc atgcatcgcg caatgcactg
     1741 acgggtgccc ccctgaacct gaccccggac caagtggtgg ctatcgccag caacattggc
     1801 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
     1861 ctgaccccgg accaagtggt ggctatcgcc agcaacaatg gcggcaagca agcgctcgaa
     1921 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc ggaccaagtg
     1981 gtggctatcg ccagccacga tggcggcaag caagcgctcg aaacggtgca gcggctgttg
     2041 ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac
     2101 ggtggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
     2161 catggcctga ccccggacca agtggtggct atcgccagca acaatggcgg caagcaagcg
     2221 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
     2281 caagtggtgg ctatcgccag caacaatggc ggcaagcaag cgctcgaaac ggtgcagcgg
     2341 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc
     2401 agcaacaatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
     2461 caggaccatg gcctcacacc cgatcaggtc gtggctatcg ccagcaacgg tggcggcaag
     2521 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
     2581 ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaacggtg
     2641 cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct
     2701 atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
     2761 ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacaatggc
     2821 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
     2881 ctgaccccgg accaagtggt ggctatcgcc agcaacaatg gcggcaagca agcgctcgaa
     2941 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg
     3001 gtggctatcg ccagcaacgg tggcggcaag caagcgctcg aaacggtgca gcggctgttg
     3061 ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac
     3121 ggtggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
     3181 catggcctga ccccggacca agtggtggct atcgccagca acggtggcgg caagcaagcg
     3241 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
     3301 caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaag cattgtggcc
     3361 cagctgagcc ggcctgatcc ggcgttggcc gcgttgacca acgaccacct cgtcgccttg
     3421 gcctgcctcg gcggacgtcc tgccatggat gcagtgaaaa agggattgcc gcacgcgccg
     3481 gaattgatca gaagagtcaa tcgccgtatt ggcgaacgca cgtcccatcg cgttgcctct
     3541 agaggcagct acgccctggg tccgtatcag attagcgccc cgcagctgcc agcttacaat
     3601 ggtcagaccg tgggtacctt ctactatgtg aacgacgcgg gcggtctgga gagcaaggtg
     3661 tttagcagcg gcggtccaac cccgtaccca aactatgcca atgccggtca tgtggagggt
     3721 cagagcgccc tgttcatgcg tgataacggc atcagcgagg gtctggtgtt ccacaacaac
     3781 ccggaaggca cctgcggttt ttgcgtgaac atgaccgaga ccctgctgcc ggaaaacgcg
     3841 aaaatgaccg tggtgccgcc ggaaggttct ggcggatcca ctaatctgag cgacatcatt
     3901 gagaaggaga ctgggaaaca gctggtcatt caggagtcca tcctgatgct gcctgaggag
     3961 gtggaggaag tgatcggcaa caagccagag tctgacatcc tggtgcacac cgcctacgac
     4021 gagtccacag atgagaatgt gatgctgctg acctctgacg cccccgagta taagccttgg
     4081 gccctggtca tccaggattc taacggcgag aataagatca agatgctgtg ataaggggtc
     4141 tttgtcctct gtactgtctc tctccttgcc cctaacccaa aaagcttcat ttttctgtgt
     4201 aggctgcaca agagccttga ttgaagatat attctttctg aacagtattt aaggtttcca
     4261 ataaaatgta cacccctcag aagaattcat tgatgagttt ggacaaacca caactagaat
     4321 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4381 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     4441 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtcattgatg
     4501 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     4561 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     4621 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     4681 acctctacaa atgtggtcat tcagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4741 ttcccaacag ttgcgcagcc tgaatggcga atggaaattg taagcgttga tatcaattaa
     4801 ccctcactaa agggagacca ctctagtttt ctttcttgct tttactttta cttccttaat
     4861 actcaagtac aattttaatg gagtactttt ttacttttac tcaagtaaga ttctagccag
     4921 atacttttac ttttaattga gtaaaatttt ccctaagtac ttgtactttc acttgagtaa
     4981 aatttttgag tactttttac acctctgctc ggtaccattt aaatctgttc cgcttcctcg
     5041 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5101 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5161 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5221 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5281 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5341 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaaccgt ggcgctttct
     5401 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5461 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5521 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5581 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5641 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     5701 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5761 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5821 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     5881 aaaaggatct tcacctagat cctttttgcc agtgttacaa ccaattaacc aattctgatt
     5941 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     6001 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     6061 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     6121 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     6181 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     6241 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     6301 cctgagcaag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     6361 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     6421 cttctaatac ctggaatgct gtttttccgg ggatcgcagt ggtgagtaac catgcatcat
     6481 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     6541 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     6601 actctggcgc atcgggcttc ccatacaagc gatagattgt cgcacctgat tgcccgacat
     6661 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     6721 tcgacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     6781 acagttttat tgtt
//
