LOCUS       Exported                4746 bp ds-DNA     circular SYN 16-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ankit Sabharwal
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        3..22
                     /label=T3 Promoter
     5'UTR           30..72
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     misc_feature    84..212
                     /label=dridh2 mts
     CDS             231..638
                     /codon_start=1
                     /label=TAL N-term
                     /translation="VDLRTLGYSQQQQEKIKPKVRSTVAQHHEALVGHGFTHAHIVALS
                     QHPAALGTVAVTYQHIITALPEATHEDIVGVGKQWSGARALEALLTDAGELRGPPLQLD
                     TGQLVKIAKRGGVTAMEAVHASRNALTGAPLN"
     misc_feature    639..2126
                     /label=co1 right TALE
     CDS             2127..2315
                     /codon_start=1
                     /label=TAL C-term
                     /translation="SIVAQLSRPDPALAALTNDHLVALACLGGRPAMDAVKKGLPHAPE
                     LIRRVNRRIGERTSHRVA"
     CDS             2322..2411
                     /label=DddA(1398-1427)
                     /note="/created_by=bmok"
                     /note="/modified_by=bmok"
     CDS             2424..2672
                     /label=UGI
                     /note="/created_by=kzhao"
     3'UTR           2723..2847
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     CDS             2994..3803
                     /codon_start=1
                     /label=Kan(R)
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(3836..4717)
                     /direction=LEFT
                     /label=ColE1 Ori
     misc_feature    join(4721..4722,4726)
                     /label=Linker sequence(1)
     rep_origin      4727..4729
                     /label=ColE1 Ori(1)
ORIGIN
        1 cgaattaacc ctcactaaag ggaagcttgc ttgttctttt tgcagaagct cagaataaac
       61 gctcaacttt ggcagatctc accatggcag gttatttgaa ggtcctaagc tctctcagca
      121 ggtctgctgc cactctatcc aaaagccccg ccgttcttgc tccagcttgc cggagtttgc
      181 aacagaggaa ctatgccgat aaacgtatcc agtctaggga cgtcactaga gtggatctac
      241 gcacgctcgg ctacagtcag cagcagcaag agaagatcaa accgaaggtg cgttcgacag
      301 tggcgcagca ccacgaggca ctggtgggcc atgggtttac acacgcgcac atcgttgcgc
      361 tcagccaaca cccggcagcg ttagggaccg tcgctgtcac gtatcagcac ataatcacgg
      421 cgttgccaga ggcgacacac gaagacatcg ttggcgtcgg caaacagtgg tccggcgcac
      481 gcgccctgga ggccttgctc acggatgcgg gggagttgag aggtccgccg ttacagttgg
      541 acacaggcca acttgtgaag attgcaaaac gtggcggcgt gaccgcaatg gaggcagtgc
      601 atgcatcgcg caatgcactg acgggtgccc ccctgaacct gaccccggac caagtggtgg
      661 ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
      721 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg
      781 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
      841 gcctgacccc ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag caagcgctcg
      901 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
      961 tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaacggtg cagcggctgt
     1021 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     1081 acaatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     1141 accatggcct gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag
     1201 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     1261 accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa acggtgcagc
     1321 ggctgttgcc ggtgctgtgc caggaccatg gcctcacacc cgatcaggtc gtggctatcg
     1381 ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     1441 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac attggcggca
     1501 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     1561 ccccggacca agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaacgg
     1621 tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg
     1681 ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     1741 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg
     1801 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     1861 gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag caagcgctcg
     1921 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
     1981 tggtggctat cgccagcaac aatggcggca agcaagcgct cgaaacggtg cagcggctgt
     2041 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     2101 acattggcgg caagcaagcg ctcgaaagca ttgtggccca gctgagccgg cctgatccgg
     2161 cgttggccgc gttgaccaac gaccacctcg tcgccttggc ctgcctcggc ggacgtcctg
     2221 ccatggatgc agtgaaaaag ggattgccgc acgcgccgga attgatcaga agagtcaatc
     2281 gccgtattgg cgaacgcacg tcccatcgcg ttgcctctag agccattcca gtgaagcgcg
     2341 gcgctaccgg tgaaaccaaa gtgtttaccg gtaacagcaa cagcccgaag agcccgacca
     2401 aaggcggttg ctctggcggc tcaactaatc tgagcgacat cattgagaag gagactggga
     2461 aacagctggt cattcaggag tccatcctga tgctgcctga ggaggtggag gaagtgatcg
     2521 gcaacaagcc agagtctgac atcctggtgc acaccgccta cgacgagtcc acagatgaga
     2581 atgtgatgct gctgacctct gacgcccccg agtataagcc ttgggccctg gtcatccagg
     2641 attctaacgg cgagaataag atcaagatgc tgtgataggc actagttagt gactgactag
     2701 gatctggcta gcgttaccac taaaccagcc tcaagaacac ccgaatggag tctctaagct
     2761 acataatacc aacttacact tacaaaatgt tgtcccccaa aatgtagcca ttcgtatctg
     2821 ctcctaataa aaagaaagtt tcttcacatt ctaaaaaaaa aaaaaaaaaa aaaaaaaacc
     2881 cccccccccc ccgcatgcct gcaggtcgac taggatcact agtgcggccg cccgcggctg
     2941 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     3001 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     3061 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
     3121 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     3181 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     3241 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
     3301 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     3361 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     3421 gcgtatttcg cctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     3481 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     3541 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     3601 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     3661 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     3721 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     3781 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcaaaaag
     3841 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3901 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3961 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4021 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4081 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4141 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4201 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4261 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4321 atacctacag cgtgagctat gagaaagcgc cacggttccc gaagggagaa aggcggacag
     4381 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4441 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4501 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4561 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4621 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4681 cgagcgcagc gagtcagtga gcgaggaagc ggaacagatc gacgatagag cgcccaatac
     4741 gcaaac
//
