LOCUS       pLKO-RevN-rtTA3-LeprPKL 9097 bp ds-DNA     linear       13-SEP-2022
DEFINITION  .
COMMENT     ^^
FEATURES             Location/Qualifiers
     promoter        1..507
                     /label="human PGK promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    525..1222
                     /label="rtTA3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             525..1222
                     /label="Translation 525-1222"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPADALDDFDLDM"
     CDS             525..1232
                     /label="Translation 525-1232"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPADALDDFDLDMLPG*"
     CDS             complement(1279..1998)
                     /label="EGFP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        complement(2021..2221)
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(2227..2547)
                     /label="Peak2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Motif           complement(2367..2374)
                     /label="SMAD"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(2865..3198)
                     /label="peak1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(3283..3306)
                     /label="pLKOseqrev1292 primer"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     LTR             3323..3503
                     /label="HIV SIN 3' LTR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     LTR             6748..7159
                     /label="RSV/HIV 5' LTR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        8593..8825
                     /label="human U6 promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    8831..8891
                     /label="scramble shRNA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatcTGTCGC CACCatgtct aggctggaca
      541 agagcaaagt cataaacgga gctctggaat tactcaatgg tgtcggtatc gaaggcctga
      601 cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga
      661 agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc
      721 acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat
      781 accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa
      841 cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct
      901 ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat
      961 tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta
     1021 tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct
     1081 tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg
     1141 gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg
     1201 acgattttga ccttgacatg ctccccgggT AAATGCATGT TTAAACGACT GCTAGCGACT
     1261 TCTagaGTCG CGGCCGCTTT ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC
     1321 GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC
     1381 GGACTGGGTG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT
     1441 GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT GGCGGATCTT
     1501 GAAGTTCACC TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT TGTGGCTGTT
     1561 GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT
     1621 CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA
     1681 GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA
     1741 CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT GCACGCCGTA
     1801 GGTCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATGAA
     1861 CTTCAGGGTC AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT
     1921 GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGAACAGCTC
     1981 CTCGCCCTTG CTCACCATGG TGGCGACCGG TGGATCCCCC AGCTTTTTGC AAAAGCCTAG
     2041 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC
     2101 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG
     2161 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG
     2221 CGAATCTTAT AGAAAATGAC TGCCTCAAGG AAAAGTGCAG ACCTCAAATT CTCCATTTCT
     2281 GTTGGAGTCC CAAGCATTGC TTGGAGGCTA ACCACAGCCA TCAAGCCATC ACTTTTGGCA
     2341 AAGTGTACCC CACCTACATA TCCCCCACCT AGACACATTT CTGCACTATG ACACAGCATG
     2401 GAGTGTCCAC TTCGCCCCAG GCTTTGAGTC AGTCGCTCCA CATATCGTGT GCATGCTAAT
     2461 TAACCGGGCA CCTTTGAGGC TCAATCCTGC TGGCGTTTTA GCTCAGCTGT GGTAAAGGAT
     2521 TACGAAGGAG TAAATGGTTA AGAAGACATC ACAACCAATC TGTTAAGAAC TGTCACCACA
     2581 CTGAAAACCA TCTCTATAAT GAGACAGAAT TTACCAGAGC AAAACAGATT AACACCCTGA
     2641 CTCACTCCAT ATTTTTGGAG GGAAAGGGGG GAAAAATCCC TAAAAGGAAA TGTAGTGTAA
     2701 AAAATAAATT AATGTGTTCA CCTATTTTCT CTTTTGAAAA AAAATCAATA GACTGCCCAT
     2761 TTACACAGAT GCAAGCCCCA CTGAGCACGA TCAATAACGG CTGAGCTTAC CATCACATGA
     2821 ACTGGGTGAA ATATTTTATC CAAGTATTTA GGTTGTCAGA TTCAGAACAT AAGCATTTCC
     2881 TGATCGCTAC GTTGGTGATC TGGATGCTGC CCTTCTTGAA TGCCTGCCAG TGACCTCCAT
     2941 GATTGCCTCT CTCCAGGATC GCCTCTTTCC TCTTCAGCTT TTACTCTTCC ACTCCTATCG
     3001 TGTAAACACT CAACTAACAG GGATGTAACA ACCACACTGA ATCACACTGG GTGACTTACG
     3061 GGCATCACAG ACTCATCCAA GAACCAGCCT TGTGGTTGGT GAATTGGACT GTGACCACAT
     3121 TCCAGGAATG AGTTGCCGGT TGTGTATCTa cacacacaca cacacacaca cacagataca
     3181 cacaTGACTG GACTTTGTGG TACCtttaag accaatgact tacaaggcag ctgtagatct
     3241 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     3301 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     3361 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     3421 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     3481 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     3541 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     3601 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3661 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     3721 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3781 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3841 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     3901 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     3961 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     4021 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     4081 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4141 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4201 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4261 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4321 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4381 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4441 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4501 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     4561 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4621 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4681 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4741 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4801 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4861 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4921 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4981 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5041 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5101 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5161 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5221 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5281 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5341 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5401 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5461 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5521 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5581 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5641 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5701 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5761 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5821 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5881 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5941 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6001 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6061 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6121 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6181 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6241 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6301 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6361 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6421 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6481 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     6541 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     6601 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     6661 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     6721 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     6781 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     6841 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     6901 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     6961 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7021 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7081 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7141 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     7201 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     7261 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     7321 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     7381 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     7441 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     7501 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     7561 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     7621 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     7681 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     7741 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     7801 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     7861 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     7921 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     7981 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     8041 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     8101 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     8161 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     8221 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     8281 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     8341 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     8401 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     8461 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     8521 ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgagcggc cgcccccttc
     8581 accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     8641 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     8701 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     8761 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     8821 ggacgaaacA CCGGCAACAA GATGAAGAGC ACCAACTCGA GTTGGTGCTC TTCATCTTGT
     8881 TGTTTTTAAT TCtcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa
     8941 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     9001 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     9061 gacagcagag atccactttg gccgcggctc gaggggg
//