LOCUS       pSYNMIBI_043_(tRNA_A37U)        2737 bp    DNA     circular UNA 11-NOV-2022
DEFINITION  metZWV A37U on p15A with Cm (from pMPE013) (pSH184).
ACCESSION   urn.local.mjeschek.3q-fja395h
VERSION     urn.local.mjeschek.3q-fja395h
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     motif           125..142
                     /label="ArgR-L-arginine DNA-binding transcriptional dual
                     regulator"
     motif           146..163
                     /label="ArgR-L-arginine DNA-binding transcriptional dual
                     regulator"
     motif           265..282
                     /label="ArgR-L-arginine DNA-binding transcriptional dual
                     regulator"
     promoter        275..359
                     /label="netYp2"
     motif           299..304
                     /label="-35"
     motif           305..326
                     /label="CRP-cAMP"
     motif           323..328
                     /label="-10"
     motif           331..345
                     /label="Fis"
     promoter        353..437
                     /label="metYp1"
     motif           377..382
                     /label="-35"
     motif           400..405
                     /label="-10"
     motif           413
                     /label="+1"
     tRNA            421..497
                     /label="metZWV tRNA"
     modified_base   458
                     /label="A-T"
     modified_base   467
                     /label="A-G"
     terminator      623..638
                     /label="Terminator"
     terminator      667..679
                     /label="Terminator"
     terminator      710..812
                     /label="T0 terminator"
     promoter        849..951
                     /label="cat promoter"
     CDS             952..1611
                     /label="CmR"
     oriT            1632..1877
                     /label="oriT"
     rep_origin      complement(1907..2514)
                     /label="p15A ori"
     terminator      complement(2632..2736)
                     /label="T1 terminator"
ORIGIN      
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt accaaaataa caccctgctt aattaactga tgatgagcct ggatttccgc
      121 tctcactgaa tttttatgca aaataaatga gttttcattt aatcatcttt tatcggagac
      181 aggaagagtt tagtgtgttt tttgtaaaat aatgcgctta agggagagca ggagaaggca
      241 aaagtattca acaaatgaaa gtgaactgga tattcattca catgattagc aataaacgtt
      301 gacaaaatgt ggcgtggatc actataatgc ctgcagattt tacgtcccgt ctcggtacac
      361 caaatcccag cagtatttgc attttttacc caaaacgagt agaatttgcc acgtttcagg
      421 cgcggggtgg agcagcctgg tagctcgtcg ggctcattac ccgaaggtcg tcggttcaaa
      481 tccggccccc gcaaccactt tcccttagag tcctttttca aatatactgt gaagacttcg
      541 gccttcgtag tgggatttga aaaaatcctt ctggaaagtg ctccagaccg cagttgcggt
      601 tatagggttc agttatataa agccccgatt tatcggggtt ttttgttatc tgactacaga
      661 ataactgggc tttaggccct ttttttatgt cttgggggtg ggcactagtc ttggactcct
      721 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
      781 ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg atggcgaaaa
      841 tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
      901 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
      961 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1021 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1081 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1141 gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg
     1201 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1261 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1321 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1381 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     1441 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     1501 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     1561 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttgacttt
     1621 cgcgcgttgt ccttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg
     1681 gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt
     1741 ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc
     1801 gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct
     1861 ctgcgaggct ggccgtaggc cggccctaga aatattttat ctgattaata agatgatctt
     1921 cttgagatcg ttttggtctg cgcgtaatct cttgctctga aaacgaaaaa accgccttgc
     1981 agggcggttt ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt aactggcttg
     2041 gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg catgacttca
     2101 agactaactc ctctaaatca attaccagtg gctgctgcca gtggtgcttt tgcatgtctt
     2161 tccgggttgg actcaagacg atagttaccg gataaggcgc agcggtcgga ctgaacgggg
     2221 ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt
     2281 ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac
     2341 aggagagcgc acgagggagc cgccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2401 gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct
     2461 atggaaaaac ggctttgccg cggccctctc acttccctgt taagtatctt cctggcatct
     2521 tccaggaaat ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag
     2581 cgtagcgagt cagtgagcga ggaagcggaa tatatccggc gcgcccagct gtctagggcg
     2641 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     2701 agtctttcga ctgagccttt cgttttattt gatgcct
//
