LOCUS       Exported               13290 bp ds-DNA     circular SYN 02-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pLenti.CRISPR.v2
FEATURES             Location/Qualifiers
     source          1..13290
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2301..2637
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:synthetic DNA construct"
     source          6342..7088
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/ApEinfo_hidden="
                     /note="/locus_tag=source:synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1990..2230
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        2241..2269
                     /label=RfxCas13d DR30
                     /note="5' processed 30-base direct repeat for an RfxCas13d 
                     gRNA (Konermann et al., 2018)"
     misc_feature    2269..2293
                     /label=Stuffer
     protein_bind    2309..2327
                     /gene="tetO"
                     /locus_tag="tet operator"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2345..2363
                     /gene="tetO"
                     /locus_tag="tet operator(1)"
                     /label=tet operator
                     /label=tet operator(1)
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2380..2398
                     /gene="tetO"
                     /locus_tag="tet operator(2)"
                     /label=tet operator
                     /label=tet operator(2)
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2416..2434
                     /gene="tetO"
                     /locus_tag="tet operator(3)"
                     /label=tet operator
                     /label=tet operator(3)
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2452..2470
                     /gene="tetO"
                     /locus_tag="tet operator(4)"
                     /label=tet operator
                     /label=tet operator(4)
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2487..2505
                     /gene="tetO"
                     /locus_tag="tet operator(5)"
                     /label=tet operator
                     /label=tet operator(5)
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2523..2541
                     /gene="tetO"
                     /locus_tag="tet operator(6)"
                     /label=tet operator
                     /label=tet operator(6)
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2588..2612
                     /locus_tag="LNCX"
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2632..2641
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2644..2691
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             2692..5589
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERYAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             5590..5610
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5623..5676
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5677..6117
                     /label=miRFP670nano
     promoter        6124..6335
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6336..6345
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6342..7088
                     /codon_start=1
                     /locus_tag="rtTA-Advanced"
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MGSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRYGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLP
                     ADALDDFDLDMLPADALDDFDLDMLPG"
     CDS             7095..7151
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7152..7748
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    7764..8352
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             8424..8657
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8689..8913
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8959..9387
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9401..9730
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9581..9716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9778..9825
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9844..10218
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10348..10469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10518..10534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10542..10558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10566..10596)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10611..10632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10920..11508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11679..12539)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12540..12644)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        12910..13289
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2041 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
     2101 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     2161 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2221 tggaaaggac gaaacaccgt acccctacca actggtcggg gtttgaaacg agacggttgg
     2281 agcatgcgtc tctttttttt gagtttactc cctatcagtg atagagaacg tatgtcgagt
     2341 ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac
     2401 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
     2461 gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt
     2521 actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata
     2581 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag aattggctag cgccaccatg
     2641 ggtaagcgac ctgctgctac aaagaaggcc ggccaggcca agaagaagaa aatcgagaaa
     2701 aagaaaagct ttgccaaggg catgggcgtt aagagcaccc tggtgagcgg aagcaaggtg
     2761 tacatgacca cctttgccga gggcagcgac gccagactgg aaaagatcgt ggaaggagat
     2821 agcatcagaa gcgtaaacga gggcgaggct ttttctgccg aaatggccga taagaacgcc
     2881 ggatataaga tcggcaacgc caagttttcc catcctaagg gctacgccgt cgtggctaac
     2941 aaccccctgt acaccggccc tgtgcagcag gacatgctgg gcctgaagga aaccctggag
     3001 aagagatact ttggcgagtc cgccgacggc aacgacaaca tctgcatcca ggtgatccac
     3061 aacatcctgg acatcgaaaa gatcctggcc gagtacatta ccaatgccgc ctacgctgtg
     3121 aataacatca gcggcctgga caaggacatc atcggcttcg gcaaattcag cactgtgtac
     3181 acatacgacg agttcaagga tcctgagcac caccgggctg ccttcaacaa caacgataag
     3241 ctgatcaacg ccatcaaagc ccaatacgat gaattcgata atttcctgga caatccccgg
     3301 ctgggatatt tcggacaggc tttcttcagc aaggagggca ggaactacat catcaactac
     3361 ggcaatgaat gctacgacat cctggcactg ctgagcggcc tgagacactg ggtcgtgcat
     3421 aacaacgaag aagagagcag aatctctaga acctggctgt acaacctaga caagaacctg
     3481 gacaacgaat acatctctac tctgaactac ctgtacgaca gaatcacaaa tgaactgaca
     3541 aacagcttca gcaagaacag cgccgctaat gtgaactaca tcgccgagac cctgggcatc
     3601 aaccctgctg agtttgccga gcagtacttc agattctcta tcatgaaaga acagaaaaac
     3661 cttggcttca acatcaccaa actccgggaa gtcatgctgg acagaaaaga catgtccgag
     3721 atcagaaaga atcacaaagt gtttgacagt atccgcacca aggtctacac aatgatggac
     3781 ttcgtgatct acagatacta catcgaggaa gatgccaagg tggccgccgc caataaaagc
     3841 ctgcctgaca acgagaagtc cctgagcgaa aaggacatct tcgtgatcaa cctgagaggc
     3901 tcttttaacg atgaccagaa ggacgcactg tactacgacg aagccaacag aatctggcgg
     3961 aagctggaga acatcatgca caatatcaag gagttcagag gaaataagac cagagaatac
     4021 aagaagaagg acgcccctag actgcccaga atcctgcctg ccggccggga tgtgtccgcc
     4081 ttctctaagc tgatgtacgc tctgaccatg ttcctggatg gtaaagagat caacgacctg
     4141 ctgaccacac tgatcaataa attcgataac atccagagct tcctgaaagt gatgcctctg
     4201 atcggcgtga acgccaagtt cgttgaggaa tacgcctttt tcaaggactc agctaaaata
     4261 gccgatgagc tgagactgat taagagcttt gccagaatgg gcgagcctat cgccgacgcc
     4321 agaagagcca tgtacatcga cgccatccgg atcctgggca ccaacctgtc ttacgatgaa
     4381 ctgaaggccc tggccgacac cttctctctg gacgaaaacg gaaacaagct gaagaaaggc
     4441 aagcacggaa tgcgaaactt catcatcaac aatgtgatca gcaacaagcg cttccactac
     4501 ctgatccggt acggcgaccc ggcccacctg cacgagatcg ccaaaaacga agccgtggtg
     4561 aagttcgtgc tgggcagaat cgccgacatc caaaaaaaac agggccagaa cggcaagaac
     4621 cagatcgacc ggtattacga gacctgtatc ggcaaggaca agggcaagag cgtgagcgag
     4681 aaagtggatg ccctgacaaa gattatcaca ggcatgaact acgaccagtt cgacaagaag
     4741 cggagcgtga tcgaggatac cggccgggag aacgctgagc gtgagaaatt caagaagatc
     4801 atcagcctgt acctgactgt gatctaccac atcttgaaga atatcgtaaa catcaatgcc
     4861 agatatgtca tcggattcca ctgtgttgag agatacgccc agctgtataa ggagaagggc
     4921 tatgacatca acctgaagaa gctggaggag aagggattca gcagcgtgac caagctgtgc
     4981 gccggcatag acgagacagc ccctgataag cggaaggacg tggaaaagga aatggccgag
     5041 cgggccaagg aaagcatcga cagcctggag tctgccaatc caaagctgta cgccaactac
     5101 attaagtact ctgatgagaa gaaggctgag gagttcacca ggcagatcaa ccgggagaag
     5161 gcgaaaaccg cccttaacgc ctacctgaga aacaccaagt ggaacgtgat tatcagagaa
     5221 gacctgctga gaatcgacaa caagacatgc accctgttca gaaacaaggc cgtgcacctg
     5281 gaggttgcca gatacgtcca cgcctacatc aacgacatcg cagaggtgaa tagctacttc
     5341 caactgtacc actacatcat gcagcggatc atcatgaacg aacggtacga gaagagcagc
     5401 ggcaaggtga gcgaatactt cgacgctgtg aacgacgaga aaaagtacaa cgatagactg
     5461 ctgaagctgc tgtgcgtgcc attcggctac tgcatcccca gatttaagaa cctgtccatc
     5521 gaggccctgt tcgacagaaa tgaggccgct aagttcgaca aggaaaagaa aaaggtcagc
     5581 ggcaatagcc ctaagaagaa aagaaaggtg tctggatccg gcgagggaag gggcagcctg
     5641 ctcacctgtg gcgacgtgga ggaaaacccc ggccccgcca acctggataa gatgctgaac
     5701 acaaccgtga cagaggtgcg gcagttcctg caggtggaca gagtgtgcgt gttccagttc
     5761 gaggaagatt actctggcgt ggtggtggtg gaggctgtgg acgacagatg gatcagcatc
     5821 ctgaagaccc aggtccggga tagatacttc atggaaacaa gaggcgaaga gtatagccac
     5881 ggcaggtatc aggccatcgc cgacatctac accgccaacc tgacagaatg ctacagagat
     5941 ctgctgaccc agtttcaggt gcgggccatt ctggccgtgc caattctgca gggcaagaag
     6001 ctgtggggcc tgcttgtggc tcaccagctg gctgcccctc ggcagtggca gacctgggag
     6061 atcgacttcc tgaaacagca ggccgtcgtg gtgggcatcg ccatccaaca aagttgagga
     6121 tccgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     6181 gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     6241 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     6301 ttctttttcg caacgggttt gccgccagaa cacaggccac catgggatct agactggata
     6361 agtccaaggt gatcaacggc gctctggaac tgctgaatgg cgtgggcatc gagggcctga
     6421 ccaccagaaa gctggcccag aagctgggag tcgagcaacc tacactgtat tggcatgtga
     6481 agaacaagcg ggccctgctg gatgcccttc caatcgagat gctggacaga caccacaccc
     6541 acttctgccc cctggaggga gaaagctggc aggacttcct gcggaacaac gccaaaagct
     6601 acagatgcgc cctgctctcc cacagatacg gcgctaaagt gcacctggga acacgcccca
     6661 ccgagaagca gtacgagaca ctggaaaacc agctggcctt cctgtgtcag cagggcttca
     6721 gcctggagaa tgccctgtac gccctgagcg ccgtgggcca ctttaccctg ggttgtgtgc
     6781 tggaggaaca ggagcaccag gtggccaagg aagagcggga aacccctacc acagacagca
     6841 tgcctcctct gctgagacag gccattgagc tgtttgatag acaaggcgcc gagcctgctt
     6901 ttctgttcgg cctggaactg atcatctgcg gcctggaaaa acagctgaag tgcgagagcg
     6961 gcggccctac cgacgccctg gatgacttcg acctggatat gctgcccgcc gacgccctgg
     7021 acgacttcga cctggacatg ctgcctgccg atgctctgga cgatttcgac ctggacatgc
     7081 tgcccggctc cggcgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga
     7141 atcctggacc gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     7201 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     7261 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     7321 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     7381 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     7441 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     7501 ccaaggagcc cgcgtggttc ctggccaccg tcggagtctc gcccgaccac cagggcaagg
     7561 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     7621 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     7681 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     7741 gtgcctgaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     7801 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7861 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7921 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7981 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     8041 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     8101 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     8161 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     8221 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     8281 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     8341 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     8401 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     8461 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8521 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8581 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8641 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     8701 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     8761 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     8821 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     8881 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     8941 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     9001 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     9061 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     9121 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     9181 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     9241 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     9301 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     9361 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     9421 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9481 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9541 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9601 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9661 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9721 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     9781 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     9841 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     9901 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     9961 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
    10021 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
    10081 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
    10141 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
    10201 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
    10261 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
    10321 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
    10381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    10441 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
    10501 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    10561 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    10621 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    10681 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    10741 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    10801 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    10861 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    10921 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    10981 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    11041 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    11101 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    11161 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    11221 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    11281 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    11341 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    11401 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    11461 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    11521 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    11581 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    11641 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    11701 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    11761 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    11821 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    11881 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    11941 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    12001 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    12061 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    12121 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    12181 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    12241 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    12301 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    12361 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    12421 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    12481 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    12541 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    12601 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    12661 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
    12721 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
    12781 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
    12841 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
    12901 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
    12961 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
    13021 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
    13081 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
    13141 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
    13201 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
    13261 tctacgtatt agtcatcgct attaccatgg
//
