LOCUS       pGGA000+SCOOP12         5588 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature    201..1061
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2212..2230
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2248..2251
                     /label="A Überhang"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     3'UTR           2254..2491
                     /label="3'-UTR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2254..5153
                     /label="SCOOP12p"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     5'UTR           5060..5153
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    5154..5157
                     /label="B Überhang"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             5154..5440
                     /label="LacZ Reporter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(5175..5193)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc ttcaataggt ttcgagtact gtattgatgt
     2281 ttaactgtat tgtatatgta tttaattttc tattccctgc catcataaat caagctttgg
     2341 aaaagtctca aacatgtata atgtctttcg tacacaatgt tttacgttct tttttttttg
     2401 ttttcatttc aatataatga tagaagtttt ttttttgtgt agcagagata tgtaaagata
     2461 atcagattga aactagctag agaaccaaaa atatttaaat gcaaacaaaa ttaaagattt
     2521 tatatatgaa ttacgtaaga ggaaacaaaa ctaaactaaa gtaggaagaa catgttcact
     2581 atagagtaac tctttacaaa ctatttctta ttgataacaa acttgattta ttctcacacc
     2641 accgcataaa aaacgagtat ttttaacacc gaaaaccatt atctaacgat atgataatgt
     2701 tcaaaaaaac cttttgacat cgctaaatgc tcgtaaagta gtcgttaaat attctgttac
     2761 gattatttga caactaaata tcgtaaacga aaatcgagag tcgctaaact tattagactt
     2821 attattaatc gtcttgtaac gactaataat aaatcagcca ctttagataa agtttttttc
     2881 atactgctat cgtgtcacca atataatagc ctttttctat ctgaaattat atattctaca
     2941 ttagctatgt tacgtaatac atatttggaa actatcgaaa tatcttgttc ttattaaaac
     3001 ctcatccacg attatcttaa ccggagcact agccataacc atattcattt cctcatcttt
     3061 actccataat ctaagtcact ttagcagtaa cataatagaa atgccttcta tctagtgatc
     3121 ggttttgact atctcaaaaa tatttatatg caagagttat ttaatattat atattcacat
     3181 ttaaccagaa acaatctggg attcagatat atgactttaa atatatgggt ttaaataaga
     3241 tcaggaatta tggattcaaa tatttgacag ctgttacacc atgaatgata agattggatc
     3301 agagctagta tctaaaatct agaactattt ttcattaatt actactttgt agagaagaat
     3361 agtcaatgag aaacaaaagg gttaatattt tcatacgttt atcatctgtt ttaataagaa
     3421 caagagcgtc aacttagttc cggactcttg atcctagctt aaagagttta ttattattat
     3481 agttccacat tcaaccaaaa acactctcgg attcagatag gaacaagagg gtcaacttct
     3541 taaagattaa tgactagtgg agaaattacg aagtttgcct tgtatttttc tttagtattt
     3601 gtttactctc taccgcccta ccggcgatgc ctcaccattc gggtacataa atgaaattct
     3661 gatatcaaga aaaattggaa tctattttcg aaaagtcata ctagcgtttt ctaccttttt
     3721 agttacttac attttttttt ttcgtacctt tttagttact tacataaaat tccgttttag
     3781 atgtcttttc ttttttaata aagaaatttc ttgtatcgtc tcttttctgt gtttcagtaa
     3841 tttatgtaca tatacacatg tgccaagtat atcttatcca gataagcttc agattttgta
     3901 agactggtga ctttaaattt taaaagaaaa caaaaaagtc tgttgacttc cttattgtaa
     3961 tagaggtcaa agtctcaagt ttgaccatta atgtaaataa ttatccattt ggaaatatct
     4021 attttagtcc aatagaaaat tgattcagta aatgattctg ataaaatgcc cgtatttcca
     4081 tgaggcactt aatgattaaa ttctcatagt gtttgaactc caacttcaac ataaacaaag
     4141 agaaatcttc aatggatttt gcagctgcat gattaagaaa ttttgaataa gcattttgca
     4201 agaagtagtc cctcttgaaa tttatgaaat gctatgtaca accgcatttc taatctatag
     4261 caaagccggc caaaactata taccatcaac cacatctctc tgtttctcga tccattcgtt
     4321 tggcaaaagc tgctggattc cttttgtttt cgctctatta cagttattcc tctagtcttt
     4381 tgttacatca aactctaagc tttcacccaa atgaatttgg tcttgatttt cctctggatt
     4441 ggtatttgtt tggcaataaa gtttcaactc aaaccaaaat acttatcaaa atatcttgca
     4501 atgtcaaaga tatttgataa gcttcataac tatcttaacc ggatcacgtg gcataaccat
     4561 cttcggttcc tcaactttac cctttcataa tctttgtcac ttaagtagta acataacagc
     4621 gttcttttga atgctttcta tatagtgatg ttgttcatta ttccctatcc caaaaaaaaa
     4681 cttatatgca aggacttatt taatgttata tttacatttg tttagctata gtatattttt
     4741 acattcaacc aaaataaaaa ttcggatcct gatatatgaa cttcaaagat agtggcttaa
     4801 gatcacgaac ctcagattca aatttgtaag gagctatgaa aatattaacc ttagatcgga
     4861 tctgaaaatc ctgaactatt ttatgtcacc atacttagta agaaactaat ggtcaattaa
     4921 caacaaacta aaaggattaa tatttgtata cgtaattctt tgtttttata agacaagagg
     4981 gtcaacttgt taaagggtaa tgatcagtga gtattattga ctagtgaaga aaactaaaaa
     5041 gtcaacttgg tattattagg ttttggtttg tttcattttt tcgtggaacg ttccctccac
     5101 ctgcatgtac ataaatatga gtctaaactc gagaaaaaaa taaagatcga gaaaacatga
     5161 gaccgaattc tcgccctata gtgagtcgta ttacctggcc gtcgttttac aacgtcgtga
     5221 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     5281 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5341 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     5401 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5461 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5521 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5581 acgcgcga
//