LOCUS       pGGA000+BAK1            4415 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature    201..1061
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2212..2230
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2248..2251
                     /label="A Überhang"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     5'UTR           2252..2736
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2252..3982
                     /label="BAK1p"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     5'UTR           3494..3982
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3983..3984
                     /label="B Überhang"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             3983..4267
                     /label="LacZ Reporter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(4002..4020)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc ttgttttttg gaaacagaga gaaaactcaa
     2281 aagcccatga agcaagctct gagataaaat tacaaacaga agaatgctaa agtgggactt
     2341 ttagatggtt ggagagaatt atgcatgtgt gtggcaatga ttagcgtagt gtggtggtga
     2401 caacaaacaa aggtggagga agatagaaag tcgatagata ccttacctcg aaaattcgtc
     2461 tctctctttg attatttttg tatatataaa tagaaaaaag tagtagtaaa taagaaaatt
     2521 ggatacagac acagatatgt cgtgaaaagg gcactaaaat attggatggt gcatgtgaat
     2581 cgtcaaaaag tggcaaggag aaagtgaaag tcttcacgaa ttatattttg gtttatatat
     2641 aaaagaagaa gaaagaagaa gaaagaagaa gaaagaagat ggatgataca caatcggtca
     2701 cggactcacg ggtaggaagg attgtgaggc gttacaatct cgtcagactt ccaagtctta
     2761 atctgatggg cctttaaata gcctactgta ctgagttgtt tgttgtgttt ttgttttgtt
     2821 ttttaatgcc aaactaaggt atatgtccgt attttattgc tggcaacata tactctgtcc
     2881 gtttgtgttt tttgtttttt atttcatttt tcctcaaaat tacttttgtt cttttaaaaa
     2941 tattaaatgg cataaatagt taaactatca accagtaaat tatatatgat acaataatgt
     3001 tttttgtttt gtttaattca ttggaccaga tggattattc agcttacatt tttcaatatt
     3061 gaattgtaat tttttagagc tataatcttt aaaaaagaat aaaaaaatca aaaaagcttt
     3121 tgctcatctt tggaataaac gaacttgcta ttaagaccaa gcccaataaa caataaaagc
     3181 ccgaaaaaat agagctgggc cggtaattag gattatgatg atggtatata actcactatc
     3241 aactatcatg agttcatgac catcatggtt tatatctaat gtcatatgat ggatgatgat
     3301 aaggtgggcg aacataacaa acatgattcg gaatgaagtc gtgcgtcttt gactttgact
     3361 agctctaaaa aagctttgaa aatgctcgtc cacgtcatca tcctctttgt cgttttctca
     3421 atcttaaatg tcattttttt aatatagttt tcacagtaca acaactcaac ccagtaaaat
     3481 cccttcttta agcaaagaaa ataattaaaa ttcgtcttcc ttccttgctc tcggcgataa
     3541 cttggtttct ctcctctctc tcatctctct ttgtttcgac ccttttttag tatatttcca
     3601 ggaaatatct tcttcctcct ttcgttttct ctatctcagt tttctctctt ctcagcatta
     3661 agtagtcaac ggtcagcgat ctcggcgttc cttctaatcg gaaaagtcta gcttcagttt
     3721 cttttttttt tgcttttttg gtttccgcga ttaatcgatt tgggtatttt gattttctct
     3781 tcaaattaag tcaacgggtg gatacgcgtt gagagggctt ttctcgtatt ctgcttctaa
     3841 tttcatcatc ttggtattac cttgtgtggg tggtagctta atcgaaggat tcgagatccc
     3901 ttttatcagg ggttttaaca atgatggatt ttctctgatg agggatagtt ctagggtttg
     3961 tttttaatct cttgaggata aacatgagac cgaattctcg ccctatagtg agtcgtatta
     4021 cctggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     4081 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     4141 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     4201 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     4261 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4321 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     4381 tcagaggttt tcaccgtcat caccgaaacg cgcga
//