LOCUS       pGGA000+FLS2            5597 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature    201..1061
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2212..2230
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2248..2251
                     /label="A Überhang"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     5'UTR           2252..2273
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2252..5164
                     /label="FLS2p"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     tRNA            3671..3750
                     /label="pre T-RNA anticodon"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     5'UTR           4938..5164
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5165..5166
                     /label="B Überhang"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             5165..5449
                     /label="LacZ Reporter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(5184..5202)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tttttttgtt agagagattt ttgttgtttt
     2281 tgttgttatg gttagtactt ttactccgtt ttatagatgg atcgggttaa atttgttcaa
     2341 tacataatga aatgtctatc tatgttggta atttctttcc actaaaatat ttgaatttct
     2401 ttttacatgt atttattttc aactattacc attaatttct ttattagaaa agtcatattc
     2461 aacatgtttt atcaaaactt tctgtcaaaa gatgatatta gaactaaatt cggacaatat
     2521 tttagataat tcctcgtttg cgacgtaata catgatgtca aaactatgtt acatttagta
     2581 gtacttatct tgaattaaat aaaattaaga aaaaaatatg aatggggtgt tactatggca
     2641 tatatctatt acctctggat ttcattctat atacaaaaat attattttaa aatgataatt
     2701 tacacgcgcc aaatatgcgt accagccacg aggcaaaaat tactatataa ctttttttga
     2761 aaataaaaac taattttttg ttttttcaaa ataagataca aattatacgt aattccttag
     2821 taaagcctga aaaacatcaa actttatttt ggctaaagga catcataaaa cctactatgt
     2881 aattgtcatt aacgtttgtt taactaacca aaataaatta ctgaatcttg aactataaat
     2941 tcgaatatag agaaaatatg aaattttgta agtgttatta gacatccaaa ttttaaaacc
     3001 gcgtaaacta cactacaatt taaaatcgga gtatgtaacc tacatagagc ctaaccacct
     3061 cctaagccgt atttaataac attgttatat tatgtaaaga tatgatatat atcgttaagt
     3121 aatattatgt agtgaaaatc cgtcacaaat cataataact tgtagtgatc aaacttcttg
     3181 tatataatct actttgttct cgcgagcacc ttaatcaatc atcccattta aaaaatttct
     3241 ttttgctatt aacatcttaa atgttaaatc tatcattgag caatgtaaca gttctgtgtg
     3301 aagaaacaag gaatcactta cttctacatt gtctctttac aaaggaaatt tggcacatga
     3361 taatgcttcg gtttgggcaa tctccttata tctttgcaga cttgtctatt accataggta
     3421 atcatataaa agttattata tatgaacaat gttcctatgg taatagaaat tcacatttta
     3481 ttaaaaaaac tgatacgaat aattcaaaga aataaaataa cttttatatg attgtccata
     3541 agatatagtc aaaacgcaaa agactttgct ctaagaaata ttaaaaagaa aaacaacaag
     3601 acgaggatta tttcttttca ataattaaaa cattagtata aatacaatca catgcttaat
     3661 gtaataatgt gacagtttgg ccgagtggtc taaggcgcca gatttaggct ctggtccgaa
     3721 agggcgtggg ttcaaatccc acagctgtca atttgttttt ttctttgact cctttgttca
     3781 tctttatttt aaaatcaata taaacccttt tcggacattc taaatatttt gacccaacct
     3841 aatgatattc attatttgtc taacgggtca actttcaagt cttcaagtaa acatgatatg
     3901 gaattatatg ggtcaactcc aagtaagaac agtgcatgca tcttgaagac aaaattgatt
     3961 tttttttttg tttttaatta atggaaattt tgaaatacta atgggtcaag atgaggttgt
     4021 ccaaatcaac tgatatcata tatgaccaga ttcctcaata gtcattagtc aatacaaaat
     4081 ccataatctt ttttaaaaat ataatctttt tattttaaat cgcctatttt tttccattca
     4141 tttgtccaat gtgaaaagcc ataaatcata tattaagaag ttgtgaattg tgatcatagg
     4201 agtaatattt ctatctgaac aaaaattgaa ttttgttttg ctttttgttg aaagataaat
     4261 aaaatgtttg gcagattgtt tgtacagaac acaacttgtt gataaaacaa attccatcaa
     4321 gacagctaat gagcttttga tagtcagagc tttgaagacg tgttaatgaa ttaaactgat
     4381 tagacctaaa accaaatatt tagaaagaaa aaaatatgat aattgttatg ttatttaatc
     4441 aatagtcaaa tactatgttg agtaccaatc atttcagttt caacgattgc tactcttttg
     4501 accaataaca aaaaggtttt tatttttcat ttctcaaact ccatttttgt ttgtttttag
     4561 cataaaaaca gctagaatgt gagtttcata tgtattatat actataagaa atcatataaa
     4621 ttaacgacta gaacatattg ttttcattct agtggtatat ctgagtttga ctagaacgtt
     4681 tggaggcaga ttaaagtgat ttcgccttgt caaaactact ttgtatatgt tatcaatcaa
     4741 tcgctcaaaa ctaaatcgga aaaaaactac ataaataaga tatggaaaat gatactaaca
     4801 caaactagta agtaagtagt cactacatat tctggtccca tgaatatggc ttacatagta
     4861 catacattgt gagattaaat gaatatgata tataatacaa gcatataagc atgtgcatga
     4921 ctatgaagtg ggaatacatt tagtgccttt atatgcaact tgacagaatt accaattatg
     4981 tgaatagtga aacttatatt gcgtcttggc cttttcacat ccgtgacatg atccctcaaa
     5041 tctatatctt cttgcggcat atgcttatat ataaacatat gacttcgtct atagatttat
     5101 atccagataa ttaattctct cataatatct tctccacaat ttcaactctt ctaaagtcta
     5161 aacccatgag accgaattct cgccctatag tgagtcgtat tacctggccg tcgttttaca
     5221 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     5281 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     5341 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     5401 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5461 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5521 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5581 atcaccgaaa cgcgcga
//