LOCUS       pGGA000+PEPR2           4364 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature    201..1061
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2212..2230
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2248..2251
                     /label="A Überhang"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     3'UTR           2254..2515
                     /label="3'-UTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     5'UTR           3597..3929
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3930..3933
                     /label="B Überhang"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             3930..4216
                     /label="LacZ Reporter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(3951..3969)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc ttcagtttga gatggagttg cattgtggtg
     2281 tatggagaat aaaactgtga cccggattaa ggtgttttat ccagtggttg ttaagctgta
     2341 gagaatctgt ggtgttgcga gcgagtgtgt gtatcttggt attattacat tgttcttgtt
     2401 ttgctttctt atggtatgta atgcctttgg ttaatgtcat gtttctcttg taaaatgctt
     2461 ttgcttatat tttacgatga accgttggac ttgaaaacaa aattcctcca tcactactca
     2521 atggattcgc atttgggtct ttgggggttt tagtcttagg atgtaggtgg ccttgtgatc
     2581 ttgtgacaaa atgcaatctg caaacctcat tggaatctaa tgtgaaaacc ctttgaagtt
     2641 aaattcaaat ttcaacatca tgtaaaggaa atataccaaa gaagtggaac atatataaaa
     2701 cttaaagaaa agtgatagaa gacagaacaa ggaccaccac tctctaaatc cttatggtga
     2761 aaatcacatt ttctttgtca actatgaaaa tttatgaact acagtataat tatagggttt
     2821 ggtgatggag acatatatag aagttgttat taaattcgtt tgggacctgc aaaagtgacg
     2881 accaaccaac ttaaaatcga aatggacagt atgatatttc caaaataaac gtgttaaaat
     2941 gttacaaacg aatattgtat acattaaaga aagtatgatt cttcgttggg acttttttga
     3001 cttctcaata ccatgagacg accaaaccaa attgaattca gttaaggaat tagatagatt
     3061 ataatatgta atggtgcacg tcgaatgatt gagttatgtt ggtggaatga atatagaata
     3121 tcaatttaaa gatgatcatt gagtctaatt aagttgagaa tgcgtgttat cttattttag
     3181 taagagtaat agcatcctcc caaaatgatg acattttgta ataatgagag aaataaacat
     3241 aataattcca taaaagagag atatacaagt ggtttaagac ttttaaatag actgatgagt
     3301 gctccactat cttaatattg ttaacaggga agatcaaaat taatctctca atcagttttg
     3361 acattatgtt tttaataaac catttttgtt ttatgtgaat atggttttgt taataaagta
     3421 ctagttaaaa ttctagtgta gacgacagat accagagatc ttgattaaat tccaatatat
     3481 aatgtttatg aaagatttaa atctaacaaa gtaacagtag gatgaaatgt caatagaaaa
     3541 ttagcgtcca aagaagcttt ctcttgaata agctaagaaa acaaaacgtg gaaaatggaa
     3601 tatttaaaac cacgaaacag tctccgagta gtaatggaaa tagcgagaaa gaaacacgaa
     3661 acagcgttga aggtcgccaa ttcgtaaagt taggttcaca atctctgacg aagagttaac
     3721 caaaaagcgc gctctttttc tctctcacca aactcatcga tcgtttctta aataatgcaa
     3781 tctgttcctg tcactaaatc cagataccct ttcaaatcca aaagctctct cttttttttt
     3841 tccgcctctc attctgggtt caagggttgt tgagtgaggt tactacgtac gagtgtttca
     3901 tatttcagtc tcttgagctc taatctcaaa acatgagacc gaattctcgc cctatagtga
     3961 gtcgtattac ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4021 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4081 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     4141 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     4201 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     4261 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     4321 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcga
//