LOCUS       pGGA000+RLP29           5476 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature    201..1061
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2212..2230
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2248..2251
                     /label="A Überhang"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     3'UTR           2252..2652
                     /label="3'-UTR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2252..5041
                     /label="RLP29pro"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     5'UTR           4322..5041
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5042..5045
                     /label="B Überhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             5042..5328
                     /label="LacZ Reporter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5063..5081)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tccagcaaaa agcttcttct actcaaaatg
     2281 ctattctaaa gactcaagac cagaagtggt tcagaaacat aatgatataa gttttttttt
     2341 ccctttatct tctgcagttt tttcatgttg ttccagaaaa gggaggccat gataattaaa
     2401 ggtacagtat ccggatttga tcgttttttt tcttcttcct tcttttctga taaagatttt
     2461 cactcttcct ttaagcttat ccagcaactt ttgtcccttt tgtcttatgt tgtaagaggg
     2521 ggtaatgtat cattcttatt ttagatgaaa atcttgtgat tactcaacaa aagagtgacc
     2581 aaatgacccc ccaaaacttt gtacttaaaa tggtttcttg aatcaaaaat caaaaggtgt
     2641 ttctttaaag cttcttgtct gcttcttaga gtctttttga atggagttta attcaaatat
     2701 tcaagcaaag acagcaaaca aaaacagaga ggtcaatagt gttgagttct tgcaacagtt
     2761 actctcgtgt agattcagtt tttcccttat ttgagtttgt acaaatggtg tgacctgttt
     2821 aaatacaaca gtctaaagaa agttaataac actattgttt tccccactct cttacatgag
     2881 ctgtttcaga tcaaaatacc tgcagattct cccatgaatt ttgcagagaa ttttgacaaa
     2941 gtatgtgctt ttgtttagcg atgacaaacc actactgaat cacagaatca taaaccttgt
     3001 tttagtccat gcatggatag atagatagat acatgtacta aaatgtggag aaagcaaaag
     3061 tagatggagt ggtgtcaaat cgtaaagcac tgatgaaagg cctgagagat cgagaaagca
     3121 ttacgtggat tacataaaaa caatttacaa aaattgtcag atgtttaagg gaagtagcta
     3181 gaaatttgga ttatggtgtt gaccggtttt aaagtcttgt aaccaatcaa gtgagtttta
     3241 tggccgttgg atttttaatt ttggtgtagg gcatgcttga gctagatccg tacggtaaat
     3301 agtaggttct gaatttacct cttcagactc acagtccagg cttaagatta gatccttgac
     3361 tacatataat acacaccaga ccatatacat cacaatacat tgtacattgt cttacaaaac
     3421 atctgctgca tattgccaag tgtagtacaa tttcttaacg ttcaaatttt aacctctttt
     3481 tttacttctt acaaaatgat gttttctctg ttttaaataa ccgttatgtc gtaagatatt
     3541 cataacattc taaggtacaa caacaaaaat aatcctagga cagaaacaag tttctttgag
     3601 ttttttttgt ttgcaaaagt cttgatgggg ttgtcaaatg tatttcaatt cttctgtatt
     3661 taagttacaa ataactatga ttttgtctgg atcaattcaa actatgaata gtaaaacttc
     3721 agagaaattt tgaaaaaaag acatgaataa ttgaagacag taaatagaga gacatactat
     3781 gcccaccact tttcaagagt ttttccttat tagtagtttt tattgataac aaatatctat
     3841 agagaaaaga tgatttgaaa taatagaaaa agaatgaaac tattttgtca acagtgaaga
     3901 aactattttg ggtgattttt gaaacagttt gtaccaacta tacattttga ttgtcatgtg
     3961 gtgtgttctc ttttgaacca acaaccagat ggtcatctta catcaaatct tgattacgaa
     4021 ttggttttct taaactagtt ttcttcaaag ttaatgtata agttctaaca tgtcacattg
     4081 agatgatgac taattattca tccagaaaga atctcattct aggatcaata tcggattgga
     4141 tcaatcataa caatttcata tcggtttgaa gtagtgtgta ttcagattcc aaaatttaac
     4201 accaattaat aaatagatcc atgcgtttac tatttaagat cgttagtctc atcttatttc
     4261 atacatgtgt gaggactatc ttcctttgca atagacttgg atcatacaaa atcacattct
     4321 ttattgtaca ttttaatata gagtatgcaa aaatagtaca tggttagaaa ctaatacata
     4381 tatatatata gagagagaga gacataaaac aggaaagccg ctccacagaa cctgagagat
     4441 tcgaggagct aacgtattca cgagactacc tcccaacaaa gacaagtcct tcaaaatttg
     4501 gttcgaagaa aagtgttttg aataaagaag aaacatgtgt gtatggcctt acaaaaccca
     4561 tcaagtactc tttttaattt tgtgtcttct ctttctcttc ccaatctcat gacttgagtc
     4621 cctacctctt tgtctctcac taccccacac gtgtcacttt cagtcacttc catttataat
     4681 tgcatctcac acactcaaaa cctattcgta ttctttacta gctttcttct aagtcttcac
     4741 tcttttatca taccttgttt agattctgat tgttcacgtg tctctgtctt gtcttgtccc
     4801 taatccttaa acatttcact taaactcttt taaccttttc ctaagtttct cttttgggtt
     4861 tttgctcctc gatgatgata taatctcact ttttatactt ttcctcctca aagattctaa
     4921 ttaacgtcac atctctccat tttcttttgc attggagaat gaagaacttt tatgaattct
     4981 cagtataata tcaccaagaa acacacacac tctctctttc ctctctctta caccaaaacc
     5041 taacatgaga ccgaattctc gccctatagt gagtcgtatt acctggccgt cgttttacaa
     5101 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     5161 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     5221 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     5281 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     5341 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     5401 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     5461 tcaccgaaac gcgcga
//