LOCUS       pGGA000+HPCA1           5633 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature    201..1061
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2212..2230
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2248..2251
                     /label="A Überhang"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2255..5198
                     /label="HPCA1p"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(2843..3472)
                     /label="AT5G07455"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     5'UTR           4852..5198
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    5201..5202
                     /label="B Überhang"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             5201..5485
                     /label="LacZ Reporter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(5220..5238)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tctcaaacat aagagaaaac gcaagttgat
     2281 gagggagcag aagctcaaat gtatgatatt ttcattgacc agatgaagga tggacaaatg
     2341 gatgaaatca ggctaaacat aagaaagaga gctcaggaca tccaaatatt atcactagaa
     2401 ataatttatt atgttgtaaa tgtaaatcat gtttaaatat ttatcatgca tgttgaaggt
     2461 tgttttcggg ttttccttat gtgtgttggg ttgtttttct tgtttttatt ttaattgaat
     2521 ttcctctgta tatagtggct cattttctta acatgaaata aaaaaaatgt gttcatgaaa
     2581 ttagaatatt ttgtgatcct tcttacgctg tttagctaat tttctttatc cttttggttt
     2641 ggaagtgtca aatggtgatg catttcgagt ttgtgagggt atatttgatt gccgtgtttg
     2701 ccaaggtaca tcataaggaa aacacggcta tgggatcgac cttccgaatc tctttgccaa
     2761 ggtactcaaa tacatcacca tttgaccatg gtggtggacg ccatgggatc gaccttccaa
     2821 atctctttca tttgttcctc ttaagtctta actctattag ctttccatta tgggtatagt
     2881 tcatatttgt atgcttttaa gtttctcaaa tctctttcat ttgtcagttt ttacttttta
     2941 ggactggttt attttgcacc cattccaatc gtggggtttt aagttttaac ccgtaaatta
     3001 tttttttgtt tattttgtat ttttgatacg aaagcccata aattgtgaag taaaaaggcc
     3061 caataggccc ataaaattgg gagaagagac gatcatcgga caaaccgaga aagtgaaaca
     3121 acaaagaaga aacctgactc ttcagaaact attcaggcga gagagagaga gagaaacaat
     3181 tttggacaag aacagaaacg atgtacgttt tctgtgatct caatattaga tatagtattc
     3241 cagttaatcg gaaacaaaga agcaacaatt tgcaaatcac ggaggttgaa ccactggagg
     3301 agttggattc agtctggaag gaaatccaag ttaaccaaca acacttttag ataatcggaa
     3361 cactatattc aatgactatt ttgattccag agactgtttt gcaacccaaa cagatcctga
     3421 tctcaagaat agctaccaga gtagctatga aggcagccgt gtatgatgta tcaagttctt
     3481 cacatcttca ggttcaaagc tagtaaacca tttgagaatc catccctggc aacattcttt
     3541 cctttctaag acattgaatt tgatgagctt tatacatctt aggtaatgtt actgttgcac
     3601 cagaactttg tgattccggc aataccataa tttaggagtt gtaattaatc ttccttgaga
     3661 tattatgctt gtaaaacaca tgtttaataa gttattcaag acttttccac aagtcttgtt
     3721 gaacgttttt gaagttgttg agagatatca tagaaaattc caaaaataat gaatcgctag
     3781 atcaatctat atccaaactg attattaaga cccagtcaat acacaaattg ctattctagc
     3841 aaatgtaagt ggtgaggtgc accttaggct tcaaatggcc acggcggact gacaaaacca
     3901 tttgcgggat tggatctctt gttgtttacc attcataaac tgtagtctgt ggaccgaaaa
     3961 aaaaaaaaat cgccgtagac aaactagcta ctgattttac atactgtaga aataaatttg
     4021 gtatggagct gacaacatgg tttagtccgc tccgcccgta acatccattc aaagccttaa
     4081 catataacgt agttaacgcg tagagtattt gagtgtatat atgtatcccc taaacacacg
     4141 tagttgcacg agtgtagctt gtggacacta acagtttcag tccactatca agttgatcga
     4201 caaaacatta tgttgacata catcaatagc ttgatcctac cactacaaca ttgctatgat
     4261 gtttctccac ttcttgttgt tgttgttgtc cttgctactt gtactctctt agtctcccct
     4321 agcttggtaa taaagttcat caatagccaa atttaaaggt ttggtccttg taagcttgac
     4381 acaacacaaa ttagttacac atcttcttct catagctcac atttatttat atatgttttg
     4441 catacccact cgattattaa ataattagtt ttgaagttct agtcaatatt tcatcaaatc
     4501 atcatcatca tcatcatcac tgttgttgta attagtatta gtatatcaag ttggaaaatg
     4561 gcttgtcctc atttttccaa cgaggcaacg accatgactt tattcaatct tggttgttaa
     4621 aaaaaacata tcactgcata gttgatgacc acaactatat tccacaagtc tctctttctg
     4681 agatctaacg atttgggcaa acaaaatcat attttattct ttgtttttga agtttataga
     4741 tatttttttt tgtaatcaat aatgagtgta tttaatacag tagataaata agggtcctac
     4801 tttagtttcg catcacgaca actcatatgt atactttaat gtattgtttc ataaattgtt
     4861 ccttcccatt actcaattga catttccatt taacaactct tcccaattgt gaaaccttta
     4921 tatcccatta tcagctgttt taagttgacc aagttttttc ttatggtcct cttcctattt
     4981 tcttcttttg atatactttt ttttttaact ctatttgttc aaagtcttcg tcttcttttt
     5041 gttaacatta ttgtttacat gtttttgaaa gagagattat aatttttatt aagagattcg
     5101 tcaaaccttt gtgtagagca catgcttttt tcttagatta ttttgagata ggatcagaaa
     5161 acagaggaag tgataagagg ttctttttgg gtttgaagaa catgagaccg aattctcgcc
     5221 ctatagtgag tcgtattacc tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     5281 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     5341 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     5401 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     5461 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     5521 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5581 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cga
//