LOCUS       pGGA000+FRK1            3977 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature        201..1061
                         /label="beta-lactamase"
                         /ApEinfo_revcolor="#c7b0e3"
                         /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature        2212..2230
                         /label="SP6 promoter"
                         /ApEinfo_revcolor="#9eafd2"
                         /ApEinfo_fwdcolor="#9eafd2"
     misc_feature        2248..2251
                         /label="A Überhang"
                         /ApEinfo_revcolor="#84b0dc"
                         /ApEinfo_fwdcolor="#84b0dc"
     promoter_eukaryotic 2274..3544
                         /label="FRK1p"
                         /ApEinfo_revcolor="#f8d3a9"
                         /ApEinfo_fwdcolor="#f8d3a9"
     5'UTR               3494..3544
                         /label="5'-UTR"
                         /ApEinfo_revcolor="#faac61"
                         /ApEinfo_fwdcolor="#faac61"
     misc_feature        3545..3546
                         /label="B Überhang"
                         /ApEinfo_revcolor="#85dae9"
                         /ApEinfo_fwdcolor="#85dae9"
     CDS                 3545..3829
                         /label="LacZ Reporter"
                         /ApEinfo_revcolor="#f58a5e"
                         /ApEinfo_fwdcolor="#f58a5e"
     misc_feature        complement(3564..3582)
                         /label="T7 promoter"
                         /ApEinfo_revcolor="#c7b0e3"
                         /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc ttcaaattaa acgccttttt atcaacaaca
     2281 aaaaaaagaa gaagatttga tatgttgaaa agtattaggg acgcttatta gggcagtatt
     2341 cctagttatt gcattttctt tcggtcattc gaccttagga tcatacctca atctgtatga
     2401 ctgtattcgc catgtgaatt ccaatattac atagtgacca aatttgatat ccaactaaaa
     2461 gtcgatcttt gatctaaacg aaatgacaac tatttggtta gtgattgcag gttggaaaga
     2521 tttaccttct agacctgtct tacgaagcta gtattctaaa gtaatcttca taaaccgaat
     2581 tcagaaacaa aaaaagaaaa ggagtccaaa attgtatgat catacattaa tatcagaata
     2641 gtctcttttg ttaaataaat atctgaagaa tatatatctc tttgattatt ttgtggatgg
     2701 caatgaaact aagaatatat attcattgac ttagaagtcg acaaaaaaaa ataaaaaaat
     2761 tattgactta attactagtt gaccaatata tatattatta aaagaacata ttgtatcgtt
     2821 gaaagcggat catcgggttt taaaagaaaa acacatcgtt gaaacttgaa agtgatgact
     2881 aataaaaaga tctaaacgtg tccggtcacc taccaatgtg gttttgcaaa ttattgtcaa
     2941 gtaccttgac tatattaaat aaaaaaattc accgtaacac attgatattc aactgattcc
     3001 taaaaaaata tacaaactat tgggagttgt gagatttttt atatcagtgt tggtctcttt
     3061 acatttgtga tgtggtgtta tagcatatat agtaataaac tcaaaaggaa attagatgtg
     3121 ttttgaccat ttattaaaat gaaccttttc ttgtcaaaca tttgaaaaat actagttttt
     3181 ttttttggca acgttgtaaa taatagttaa aaatagattt taagtctcgt ttttttatgc
     3241 atatagtttc attcgcttta ttagactcaa atatactttt aattaaattt tgcagagaat
     3301 taaaggtaat catttgccaa ggaaaaacca tgcaaatatg caataagtag aaataatgtt
     3361 aatgagagta agcgttgaca tatattacgt cctggtccga acattcttaa agttgcgtaa
     3421 cactaataac cttagaagat ggttggttga ctatcaacat cttattgacc aaatgttttt
     3481 ttttttttaa ttataaaaca gttgctcatt gctctagccc agagaaagca gctcaattaa
     3541 gtaacatgag accgaattct cgccctatag tgagtcgtat tacctggccg tcgttttaca
     3601 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     3661 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3721 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3781 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     3841 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3901 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3961 atcaccgaaa cgcgcga
//