LOCUS       pGGA000+EDS16           5662 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature    201..1061
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2212..2230
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2248..2251
                     /label="A Überhang"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2254..5227
                     /label="EDS16"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3649..3649
                     /label="G->C"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4179..4179
                     /label="C->G"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     5'UTR           5133..5227
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    5228..5231
                     /label="B Überhang"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             5228..5514
                     /label="LacZ Reporter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(5249..5267)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc ttcgactgca gagatcaatt ttcttttatt
     2281 ttattaaaag aaattaatca tgaatacagg atattatatt gttagaaaaa taaagaaaat
     2341 tgatttctgc agaagaatga tattcgttat agttttacaa acaaaatcat attttaataa
     2401 ttagatttag ccagaacgga cttggggttt tcatatcacc ttgcaaccag aagttattaa
     2461 aaaaaaagaa gcaaatttgc tttgtaaact catacgtatt actttttgtc gcatatgata
     2521 tatgattgat cgccactcca aattattttt gtctgaattg cagcttctgc cgcgtaaggt
     2581 aagataatca atctagcatt gcctatttgt tgtatttttt attctgacca gtgaccagcg
     2641 atcaaaagta tgggatttgg gcaacttgta tagtagttta attggactta tttcgcaagt
     2701 tgtcaaacaa tgtctcgagt tcaacccttg tagtcgatcc tccttgtgat agaagtttat
     2761 tggtcttatg tcttaagttg tcaaataaat atggagtgtg aacatttgaa ttataggttt
     2821 agtcgtagag taattatatt ttaacctcat tttctaactt gtgtgtactt tctgttaact
     2881 cttgatttat gtgtttatca tttttttaag ttatgtgttt tccattatgc tctgtttatc
     2941 tacaaatagt agtagctgaa taaccatttt tgtttttaaa aaaaaatcaa agttggactt
     3001 ccaagtccca actaaagatt aaaacatgca tgcaaataat ttttgcaagt aggattttct
     3061 ttcttaggaa ctcagactcc agaagacgac tttatttttc ccaaattgtt ttatactcaa
     3121 aggaataaat tatccacgct ttgtcacaca aaaaaaaata aaaattatat agatatatag
     3181 atatatacat gcaggaattt aaataaaatt attaatctta cgtaattaag aactttttta
     3241 ttttttttta aaaatcgtaa ttaagaactt gaatggtaaa atactagaag aaaattgtta
     3301 gcgtcagtgt gaaagcaaat tatactgtag gttaactttt ctatagtgag caaaatacat
     3361 taggttatct aaattcaact aacgtcctat ttctagttat ttgactaacc aaatcgattt
     3421 tttcgtgaag gaaaaaaaaa gtgtttagta cactcatata gtcatattct tacatataga
     3481 attcaagatt tcgaactaaa gcatacgaca ctagacttaa gtctagaagg atgacggaaa
     3541 tgagaataag ctaaaacgtt tctacgaaat taagaaattc gtagcatcca caacacacat
     3601 attcaaagtc ttgaataaca ataaaattat gttcagctga gagtctgaga ccgcgtccaa
     3661 cattttaaaa cacttttaaa agtcatacta taatatctga ggaccgtgtc tagtttcagt
     3721 ttattcggtt ttacaacagc aatgatgctt gttgaattat ggtttcattc tattggatta
     3781 tctgcaagac ttcttaattt taatatatag ctcgtgatga taaaataaca atttgacttc
     3841 taaagtctag tcctttatag tcttaacaat attcattttg accaggttga gtaaatcaga
     3901 caaaaatcta aagacaaaga ataatcgttt cctcaaatat gtacttggtg agccgtctta
     3961 atcaccatat gtacatgaga ataaatgtgt aatttggtgg ttatttcata gaaattttgg
     4021 ggaaattgtt gcacctctcc actctttgcc agtctcgtgt gatcttaatt gactcaaaat
     4081 gaaagaaaaa aaaacatgaa attaaagcaa atattcctat ttgaattatg ttcctttaag
     4141 atttttaaca atatttttta aatgataaaa actggtctca aagagcctaa gtgggtttcc
     4201 tagcaaaaaa agatactcaa agtaatttac tcctcaaaat aatgcaatga tctaatgaag
     4261 atctgttcaa atagtattga ttttccaaat ttaattatac tcaaagagtt ttaaaatctg
     4321 ttcttgttat tctatgcttt gttttacatg taaagtactt aaattcactt gatatttgtt
     4381 attactataa ttttagaaat gtgtcaaaat ttaaaggagc atgcgtgtaa tgccatatgc
     4441 cttatgtacg agaacttgta atgcgtttgc aatttgtagt gacatgtaat gttttttacg
     4501 atagtttaaa gtgcaaaccg cttccgtatc aaacgagaag agtcgtctag catacaacac
     4561 ccacacgaga ggaaccgctt agaaagagaa tccacttgaa attgctgtcc atgcattgct
     4621 caaattcatt gatacttggt ctatgcaatt ttacactacc tcatttctga ctatttcaat
     4681 ttgtcaagat attataacat gcatttataa cttttttgtc gacgataaat caaccaaacg
     4741 aatccggtct gtatgtttga taacatgcat tcatacaaaa cgattatctg ttttttagtc
     4801 tatttaattt ggtttctact ttttactttt gtgaaactaa taataataga tcaaacaatt
     4861 aactgatata atttattaat ttaattctta gaccaagtaa atgattcatg aaatattaaa
     4921 tgcacgacta actttagaaa aatgtttctt tgtatacata aagatagaat agaagaaaag
     4981 taggattaga agaaaaaaac gaaaatttca gagtagttta ctaaagaaat tctgagaaat
     5041 tttgtgtgaa aatgaaatga aaatcttcaa ttttagtggg cccctgctac atcagtcccc
     5101 tatttatatc tcttctcaac tctaaaccca aaccaaaaaa aaacagaaag agcaatctct
     5161 ctatactaca aacaccaaag tttaaaatta aacaaaatct tcaagaaaca ctttacgaat
     5221 ttctgcaaac atgagaccga attctcgccc tatagtgagt cgtattacct ggccgtcgtt
     5281 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5341 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5401 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     5461 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     5521 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     5581 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     5641 ccgtcatcac cgaaacgcgc ga
//