LOCUS       pGGA000+ERF1            5370 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature    201..1061
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2212..2230
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2248..2251
                     /label="A Überhang"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2254..4935
                     /label="ERF1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     5'UTR           4848..4935
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4936..4939
                     /label="B Überhang"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             4936..5222
                     /label="LacZ Reporter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(4957..4975)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc ttctctctcc caattgatat ttttgttatt
     2281 tcttagatag aattgatatc atgactcaag ttttaatttt ttcattcact cgaaaaaaaa
     2341 attgatttga gtccagaaat tcttttgttt caaatccaaa tggattttcc gaacgacaga
     2401 aaatgttgcc gattatacat aaataattta agaaaatcat gtaaatacat taaatttttt
     2461 taactgatat gactataaaa tcataaaaat gactagaaaa tatacaaata atatgaccac
     2521 gtaccatgaa tgtgtagcta tcgccttacg cctaacacct tagtcaaatt tgaatgctta
     2581 ctggactttg ttttctttct tttttgattt gactataaat aatcttttta tatttgccta
     2641 tttccatata aaaatttgaa tcactaagaa ttgaaattct ggagcttcca ctgaccgaag
     2701 ggaaaatatt gaggaatata aatcatttta gaaaacttgt ttatttttat atgaagtatt
     2761 agtaattatt cgaataatta tattattatt ttttgtgtca atttatggaa atcatttagc
     2821 aactgatatc aaaagcaatt attcttgggt tacttttctg atgtcaatat tgaaatcaat
     2881 taatttattc attttcggta cgtgtaagaa gtttaaagta ttttaccaca aaaaaatgaa
     2941 gtttaaagta aagccaaaag agatacaatg tccaggtttg aagtttaaaa tagagccaaa
     3001 aactttgaac acgtgcggga tatcaaataa tcagttatgc atagttttgg atgggcttaa
     3061 gaaagaataa ataattttgt gtcctactgg actcaggatg catgtgatga tgtgtagtgt
     3121 tctaatagaa tttagttgcg tatccgttcg aataattatt ttcaacacct cctacttttg
     3181 ttacattgtt ttaaatgact attgacaaaa taaaagtaaa tgtaaataac taaaacagaa
     3241 aagatgtgag aaaaactagg aaattctttt ttttgaagaa ttattaaatt tgctagattt
     3301 ggataatcga ttcaacattt ggaaaagaaa aaaaatatct aaggatggtt gaaagatagt
     3361 ttatgatcaa actagccagg attttagagg ggtcatcagt tttctgaaca tttatatgtt
     3421 gttttccgat aatactataa atttgatcaa ctaattagat gacttaaaat aatgtaattg
     3481 ggataatgtt taagaaaata ttaggtaact catataattt aagagttata atttggttat
     3541 aatttgcacc aaatttgaaa tatatttcta ctataagata actagaaata gtgaagaaaa
     3601 aaacaaagga aactagaaaa aatgaaaaaa tggcacatga agtatctttt ttgtttgaga
     3661 aagaagttga aagcagataa agccaaatta tcttctaaac atgagatggc tcgaaattat
     3721 ttgttgtttg ggattcaagg gggcatgtat cttgaatcct tccaaccaac aattttcttt
     3781 aaccatgctt ttggccaaaa gattttcttt aataataatt aataatagct tcctaagatt
     3841 ataggataat gatttgatat tttatgtaat catatttgca atacctaagc atgaaaatgt
     3901 tttgttgagt tgatccagca gttattttgg aatatggctt aatcattttc acatcgatct
     3961 tgttatttct gttcgtgaaa tgtgaacttt cgcagttatt tgttttattc ataagttgtt
     4021 ttagatcaaa taagtggagt ccacttagaa attataagat gggaatttta tttgcatatc
     4081 aaaattatct ataaaaattt tagtcaactg atttttagtt ttctactaaa atcgccatct
     4141 tattttcttc tagattcaat ttgatctgaa tgtggcatgt aggttgataa aacttatcaa
     4201 atagcatcaa atcagtattt tctaaaactt tttacatcat cataaatctt tcacttataa
     4261 aatataatat cttgggtaca ccaaagtaat cctgatatat actacaaaat ataacatttt
     4321 agttgttttc atatgtggtt aatctttttt aggtcgtaca tgattcataa accttaccgt
     4381 cataaaaaga aaaataaatt atatttaaac taagaaatgt gatatgatgc aaaagtgaca
     4441 aaatttcgat gaatcatgtg atgataatac gaaatgatat acctaaccta cactgctcat
     4501 tctacatttg aagaatatat acctaaatat acataccaat tctgggataa ttacataata
     4561 tacagtttca tattagtctc acgtaattac gattcacgac agtaagaaga ttagtttaaa
     4621 cggtacaaaa caattcatat attacgaaaa cattaaatat cacacgaaga gaagaaatat
     4681 gcaaaacgtc tttgctcgaa tcttcgacga agcaacatag tttttcattt gagtgtaaca
     4741 ttatttttcg taaattccca tttcaccctt ttattacatg acatttgtct ttcactgaga
     4801 cagtaaaaac tcaagtctaa tttggacatt tgcatataca tactcatttt tttctctata
     4861 tatatagacc atgtcaatcc actaacgatc cctaaccgaa aacagagtag tcaagaaaca
     4921 gagtattttt tctacaacat gagaccgaat tctcgcccta tagtgagtcg tattacctgg
     4981 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     5041 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     5101 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     5161 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     5221 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     5281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     5341 ggttttcacc gtcatcaccg aaacgcgcga
//