LOCUS       pGGA000+PDF12           4227 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature    201..1061
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2212..2230
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2248..2251
                     /label="A Überhang"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     3'UTR           2253..2493
                     /label="3'-UTR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2253..3792
                     /label="PDF1.2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     5'UTR           3705..3792
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3793..3796
                     /label="B Überhang"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             3793..4079
                     /label="LacZ Reporter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(3814..3832)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc ttctaccaaa aatctttggt gcttgatcgt
     2281 gtgtgtattt tactttaaat aagtcggtgt cactctatga gtgactttat gacatgcata
     2341 tatgtttatg tttttatgtt tggtttctcc ttgttataat aatactaaaa aattatgtac
     2401 gacgttggac tgtttcatca tatcccataa aaatacatga ttggggtgaa aatcttgaac
     2461 atattaaaaa aatattaaat caaaatgata aagataggga tttataaatg taaaacgggc
     2521 gtgtcgagaa ttttatggac attgggacaa gctttatatg cagcatgcat cgccgcatcg
     2581 atatcccgag gtgcatcgtt tctactttca tgtccaaatt tggggttaac tcacaatata
     2641 tatcatgttg cctatgtaaa tttataatca taaatctaaa cccaaatttt aatcctcatt
     2701 ccaaagcaaa agttctaagc cctacaaaaa tatgtatttc ccaagtttaa aaagaattaa
     2761 tctatacttt tacaaattta aattctgatc tcttataatg ttcggttttt ccttttttat
     2821 ttattaagtt agttaaaatt tgcagttatt ttgttgaatg tcgttgttta cgaatttacg
     2881 aataatacct ttatagctaa tctacaaaat tttgatgact gacaacaccg ttaatgtttt
     2941 tttttaaatt accctgagcc tctcacttgc ggtcagacca tgcatgtcga tagtccatta
     3001 cgtttaaggc cacaatcaac tatagtttgt ttatcaatag ccaactaagc taacttttag
     3061 gttcctgccc tctccgttcc tccggtacca atcgtttctt tgtcccttcg atagtttgaa
     3121 aacctaccga cggtgagagc aaaatattga tgaatcatcc aattttcagt aataggtgtg
     3181 tcccagggat atataaatgg cgaaactacg cgagaacggt tccttgttct gcaaacttgg
     3241 cggaacaatg ctgctcttga gatcaaccaa accatatgtt tagtccacaa cgatctatat
     3301 gtctaggggt gatcctctaa tcgaaaaatg ttgtatttgt tcgacgatga cgaaggtcag
     3361 actatgaact gcacagtctg cacttgtcct aaccgcgaga atctctgaca tcaatatact
     3421 tgtgtaacta tggcttggtt aagatattat tttcttgagt cttaatccat tcagattaac
     3481 cagccgccca tgtgaacgat gtagcattag ctaaaagccg aagcagccgc ttaggttact
     3541 ttagatatcg acagagaaat atatgtggtg gagaaaccag ccatcaacaa acaaaaagca
     3601 agatcttatc ttttgatatt ggctacggga agatgatgtc tgtttaatgt gtggggttac
     3661 cacgttattg tacgatgcac aagtagaaga ttaacccact accatttcat tataaataga
     3721 cgttgatctt tggcttattt cttcacacaa cacatacatc tatacattga aaacaaaata
     3781 gtaataatca tcaacatgag accgaattct cgccctatag tgagtcgtat tacctggccg
     3841 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     3901 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3961 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     4021 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     4081 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4141 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4201 tttcaccgtc atcaccgaaa cgcgcga
//