LOCUS       pGGA000+FMO1            4411 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature    201..1061
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2212..2230
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2248..2251
                     /label="A Überhang"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2252..3976
                     /label="FMO1p"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     5'UTR           3835..3976
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3979..3980
                     /label="B Überhang"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3979..4263
                     /label="LacZ Reporter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(3998..4016)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tcggaaaaat ccttcgtcaa tgtgtgctta
     2281 tacaattcat agttttgggt gatagtcaaa tataagtgat aaggctacct agactttttt
     2341 ccattgtttt gaaattcaac acttgcatgg acacgtactc gattattatt ttaattcgct
     2401 tttagggata aagatttagt caaaagtaaa ctagagacaa aaaataaaat aaataaatag
     2461 agatagtagt agttatagtt caagaacctg acagaaataa cagattaaga atagaccaat
     2521 aatgattaat taaaatacat ttataaagtc tatccaccaa agaccatata aagattatac
     2581 tttttttcgt tttgtttttt ctcgttgata aattaaactc aagatcttgt aatgtatgag
     2641 aatagtaaga gtaagggctg catatagcaa tttacatgaa ttaaaggcgc tctactcgta
     2701 tcaccatata cttctatgct ttacagttta cctaattcac catgaactaa ctatatatat
     2761 cataattcca cccaaactat taaattcatc acacatagaa aatttcaatt cacctctgta
     2821 catgctcttc aacggcttgt taataaggga tggcttcaat ccggacccgt ttcttgtgcg
     2881 gaaatcaaac cataatcaag attgtctttg tcgactatgt taattatttt tttaaggatg
     2941 tgtcttatgt acattgtaaa aattatgatg ttcattttat ttgtcttact tcattgcgga
     3001 gaacatttcc tttattgatt aaaaacaata attatattgt tctttcaaga cttaaacgtt
     3061 agaaagctct cggaaaaaca aacattttcc cgtgtattaa gctgtacgta tatgatgtta
     3121 taattatctc aagaaagcat acatattgat tgtaaataaa aagagtagac tagaacacat
     3181 gtgttaagtt gagttagatt cgctaatgag cgatcaaatt gcttacatac agtcatccgt
     3241 tctatcacgc acgatatttc tcgataaaca actatccata aaatttagat gtcagataat
     3301 ttttgtaagt aaattcgcaa taaacatata tatatatata tatatatata tcaattttgt
     3361 caccgaggac aatggaattt tctctcattc ttataatatt ctcaaatgat attcgagagt
     3421 acattttaaa taggaaggag atttcgataa cgatattaat ggaaccaaga atttgtttta
     3481 aacctatttt tgatataaaa aaatctatgc atttgtatat aatacagttg ggtcaattta
     3541 tagactaaga agctcttaat tgttcatatt caagaagaca aagcaagtta aaaacctaat
     3601 ttaacaaaag agaaaatatt ccattagact tcttgaaagt acttagaaat ttaacacgaa
     3661 caaatacaca aagcgaattt ttaatatatt tctcacgctt gcagaaattt cctcacaata
     3721 accacaaaat ttctcagcga taaaaaaaaa acagtttaga tctttcaaac ctactcgaaa
     3781 ctaaactaat attcaaaagc acgtgctccc taataaaact cattcctgac ccgactacac
     3841 aatacccgac ccgacccaaa aacatcttat aaatatgttc atgcaaactc acaaacaact
     3901 caaaacactc ttcatcaaaa ccattcctct cctcaaaacc attaatctct cttctctcta
     3961 gcataacctc tctcagaaca tgagaccgaa ttctcgccct atagtgagtc gtattacctg
     4021 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4081 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4141 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     4201 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     4261 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4321 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4381 aggttttcac cgtcatcacc gaaacgcgcg a
//