LOCUS       pGGA000+CSLD2           4649 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature    201..1061
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2212..2230
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2248..2251
                     /label="A Überhang"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     3'UTR           2252..2476
                     /label="3'-UTR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2252..4214
                     /label="CSLD2p"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     5'UTR           3143..4214
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     intron          3811..4189
                     /label="Intron"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4217..4218
                     /label="B Überhang"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             4217..4501
                     /label="LacZ Reporter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(4236..4254)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgacgaccaa gactagagtt ttggttcggg
     2281 aatgactcca aatgcacggt ggtgatctct gatctgtgac caaatgtatt tctcgttttg
     2341 tgtcttgcaa acaaaacata ataaatcaaa ttaagtcaat tgatgttttc aaatccgatc
     2401 cgcttgacgt gatccgctga taatattggt ctgcgatggg gtgtgttgtt ggttcgtttt
     2461 gtgtaatagc gtggattaat attcactcat cacataaatt ttcaaaaata acaacaaatt
     2521 aaatactata tgtcagatat ttaatattaa tgttgacagg gacattatta cgtgtataat
     2581 gagaattatt cgtggttagt agtagttact cccatagata tgaaatattg acatttttga
     2641 tcaatgctac gtacacacgt aacacgttat ctcattattc caaaaagaaa cattttgtat
     2701 tcacaggtga gaaatactcc tgcgaccgcg tattcccatt ccttattttg aaattcccca
     2761 aagtaccctc atcgtctctt tcagtgtgct tacacgtttt ttgatacccg agattaacgc
     2821 ctccctacga cattcttttt tattttccca tattttattc tgcaaaatac caaaaacaaa
     2881 attgctttat actaacatga tatattaatg acattaatct aaacaacgat atattaatga
     2941 cattaatcac aaccatttcc cctttattat aagaggattt cgtaatctta catttcttaa
     3001 tgccattata tttttaactt gcgacttccc gagaaaattc aaattcactt gagttatttt
     3061 caaaacttgg attctacagt atctgtttgc tataaacact tgtaaatcac tgaactgaga
     3121 tcaaataaat tagagatttc tttaatcttc tatggtaagt aaattttcgg tatccctatt
     3181 caagaaagag aaaaaaaatg gaaacaatat ttaacgcgtt aaaagataaa taagaccaca
     3241 agagttaaaa agagttgttc ttactttctt ctctaccttg tccgaaaaac ctcagtcccc
     3301 cccccaaatc aattaaaacg tttcaagaaa aaaaaaaaaa agtgtactac gtcaacttgt
     3361 tttttccggc ggcgttccac cttcgtcttc gctgtctcac cgcgtcataa aattttctac
     3421 cttagtaaaa atttcgaaat agatcgtaga gatcttcctt cttgatttgt cgtctcgatt
     3481 ctacaaaatt tggcaatttc tttgttttat atatatttta agatctttga atatatattt
     3541 tggggataaa tcggctaacg tgtattagtt agcaggagtg ctcaagtatt tgctcacgac
     3601 taagtccttt tctcctcttc ttcttccttt cactatctct tcttatcaaa tgcctcataa
     3661 taatatccag ttgggtggca cgacacgacc gcgttgaata cttcagacgg atttgttaac
     3721 aacaaagtct ctacttcttc ttctgttaat tactatataa ctcaaagatc tgagtttctt
     3781 ctctcttctt agcgttcctt tcctctgaag gtattgtaag caatctcgag tttcaaatct
     3841 tgttttattg ttttcttata agttcgtggt gggatcgtct ctttttgtct gaaaatgtct
     3901 aaagcctgtg cttaaagaaa gatgtgtggg cttttcgaga tattctcgga tttgaaagtt
     3961 tgaaactttg aattagtgta ttcgtcttga taactttaaa ggtggtgcct ttttgttgta
     4021 gtaagcaatg attgttacct tttcttttca tttgtgtgaa tgttgatgat gagtttgttg
     4081 ttagatcgat atatatatat tgatctgttt ctttgttgat gtttttgaat gaatttaaga
     4141 tgtctttgtt tcttcttttg ctaattttgt ctttgacttc tttctgcagg gatctgccaa
     4201 gttagatcct aactaacatg agaccgaatt ctcgccctat agtgagtcgt attacctggc
     4261 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4321 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4381 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     4441 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     4501 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4561 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4621 gttttcaccg tcatcaccga aacgcgcga
//