LOCUS       pGGA000+ATG8A           4095 bp ds-DNA     circular     24-MAR-2023
DEFINITION  .
COMMENT     pGGA000
FEATURES             Location/Qualifiers
     misc_feature    201..1061
                     /label="beta-lactamase"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2212..2230
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2248..2251
                     /label="A Überhang"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2254..3660
                     /label="ATG8Ap"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     5'UTR           3406..3660
                     /label="5'-UTR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3661..3664
                     /label="B Überhang"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3661..3947
                     /label="LacZ Reporter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(3682..3700)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc ttcttagcag tgcttagtga gcttaaatta
     2281 tagtttagca aaacaaaatg gcatatactt tggaaccttt gaatgtgaga aatttgaaag
     2341 cactctcgat ctcttcacca ttttggcctc gaccaagaga gaaaaatgta gcgaataatg
     2401 ttaacatttt tggtccgaca caaggttaag gggctcgttc catattgctc actacctttc
     2461 acttttcaat gttatatagc aactagacat ggtgttacta ggggaatggg gtatatgtat
     2521 ctgttatcta tttcggtgtg ttgatttaat tttttacttc gtgtattctt gaaccaatct
     2581 gcaactgaaa aagttgtatt tgaatttgga ttaattcgtt tcgttgctaa atatccattt
     2641 caagatatac ataataaata tattagttaa tttactatgg taagatatat tttcaagaat
     2701 gaagttgtat aaataaaaat acttgtttcg ataattttca gttttattca cgtcggtact
     2761 atctcacccg tactaaattt gaattgccga caaacaaaaa aaatgtcaag ttcggttgga
     2821 ttttttattt tgtttggatc tactcaatat ataaatagta ccaatcacac tataaacgaa
     2881 atttactcat ctttccttgg ccctagcaac ggttcatgat aatcattctt tctttttaca
     2941 atttactttt tcgaaataaa aaattgtagt aaaattacag tttcaactaa aaaaaaatct
     3001 tgtacagagg tgccacaaaa atgcaattca attttatata aaaatacaac gacaacaaat
     3061 ttgttacttt gtcaaagcgg attatttggc acgaaaaatt cgtgcggaac cgctacatat
     3121 catttttgta aataattttc ttcaaaattg gctcgtttga gtcataaata acacctgatg
     3181 acaaaaaaaa aaaaaaaatt tttttgttac tttgtaaact tttgctaacc aaagcctata
     3241 taaaggccca attagcccaa aacgacacaa aaatgtgtga agaaataaac agatggtggt
     3301 gacgtaagac aagtgtaaga gatttcttgt aatccaatca acaaataata ataaacgaat
     3361 tccaagtggt ttacttattc cagaaacagt tccgtgtagg caaccattag gaaaacccta
     3421 actaaattcc attattaatt tcttttataa tccctttctc ttgacattga cttctctctc
     3481 tcgattcttc ttctccagtt tcaatcaaac tcaatttctt ctcaacgaac cctaatttcc
     3541 aacaaatcgg attcagtttc ttcttcgggt atgtttcttc tcaccaataa attcgagatc
     3601 ttttagggtt ttctgggttt gtttctcccc cgatctagca gacgatcgag tttattaatt
     3661 aacatgagac cgaattctcg ccctatagtg agtcgtatta cctggccgtc gttttacaac
     3721 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     3781 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3841 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3901 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     3961 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4021 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     4081 caccgaaacg cgcga
//