LOCUS       Exported               15246 bp DNA     circular SYN 12-JAN-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15246)
  AUTHORS   Macapp123
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15246
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    265..289
                     /label=LB T-DNA repeat
     polyA_signal    367..541
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(567..1118)
                     /codon_start=1
                     /label=BAR
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1157..1834)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1926..3754
                     /label=SlEF1a Promoter
     CDS             3759..7847
                     /codon_start=1
                     /label=LrCas9
                     /label=LacCas9
                     /translation="MTKLNQPYGIGLDIGSNSIGFAVVDANSHLLRLKGETAIGARLFR
                     EGQSAADRRGSRTTRRRLSRTRWRLSFLRDFFAPHITKIDPDFFLRQKYSEISPKDKDR
                     FKYEKRLFNDRTDAEFYEDYPSMYHLRLHLMTHTHKADPREIFLAIHHILKSRGHFLTP
                     GAAKDFNTDKVDLEDIFPALTEAYAQVYPDLELTFDLAKADDFKAKLLDEQATPSDTQK
                     ALVNLLLSSDGEKEIVKKRKQVLTEFAKAITGLKTKFNLALGTEVDEADASNWQFSMGQ
                     LDDKWSNIETSMTDQGTEIFEQIQELYRARLLNGIVPAGMSLSQAKVADYGQHKEDLEL
                     FKTYLKKLNDHELAKTIRGLYDRYINGDDAKPFLREDFVKALTKEVTAHPNEVSEQLLN
                     RMGQANFMLKQRTKANGAIPIQLQQRELDQIIANQSKYYDWLAAPNPVEAHRWKMPYQL
                     DELLNFHIPYYVGPLITPKQQAESGENVFAWMVRKDPSGNITPYNFDEKVDREASANTF
                     IQRMKTTDTYLIGEDVLPKQSLLYQKYEVLNELNNVRINNECLGTDQKQRLIREVFERH
                     SSVTIKQVADNLVAHGDFARRPEIRGLADEKRFLSSLSTYHQLKEILHEAIDDPTKLLD
                     IENIITWSTVFEDHTIFETKLAEIEWLDPKKINELSGIRYRGWGQFSRKLLDGLKLGNG
                     HTVIQELMLSNHNLMQILADETLKETMTELNQDKLKTDDIEDVINDAYTSPSNKKALRQ
                     VLRVVEDIKHAANGQDPSWLFIETADGTGTAGKRTQSRQKQIQTVYANAAQELIDSAVR
                     GELEDKIADKASFTDRLVLYFMQGGRDIYTGAPLNIDQLSHYDIDHILPQSLIKDDSLD
                     NRVLVNATINREKNNVFASTLFAGKMKATWRKWHEAGLISGRKLRNLMLRPDEIDKFAK
                     GFVARQLVETRQIIKLTEQIAAAQYPNTKIIAVKAGLSHQLREELDFPKNRDVNHYHHA
                     FDAFLAARIGTYLLKRYPKLAPFFTYGEFAKVDVKKFREFNFIGALTHAKKNIIAKDTG
                     EIVWDKERDIRELDRIYNFKRMLITHEVYFETADLFKQTIYAAKDSKERGGSKQLIPKK
                     QGYPTQVYGGYTQESGSYNALVRVAEADTTAYQVIKISAQNASKIASANLKSREKGKQL
                     LNEIVVKQLAKRRKNWKPSANSFKIVIPRFGMGTLFQNAKYGLFMVNSDTYYRNYQELW
                     LSRENQKLLKKLFSIKYEKTQMNHDALQVYKAIIDQVEKFFKLYDINQFRAKLSDAIER
                     FEKLPINTDGNKIGKTETLRQILIGLQANGTRSNVKNLGIKTDLGLLQVGSGIKLDKDT
                     QIVYQSPSGLFKRRIPLADL"
     CDS             7860..7880
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7893..7913
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7926..7946
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /note="SV40 NLS;color: #993366"
                     /protein_id="PKKKRKV"
                     /translation="PKKKRKV"
     polyA_signal    7956..8005
                     /label=polyA polyA signal
                     /note="polyA"
     misc_feature    8006..8082
                     /label=tRNA-Gly
                     /note="tRNA-Gly"
     gene            complement(8090..8395)
                     /label=ccdb
     5'UTR           complement(8396..8714)
                     /label=ccdb 5' UTR
     misc_feature    8722..8860
                     /label=gRNA Scaffold
                     /label=LrCas9 gRNA Scaffold v2
     misc_feature    8861..8937
                     /label=tRNA-Gly
                     /note="tRNA-Gly"
     terminator      8944..9193
                     /label=HSP terminater
                     /note="HSP terminater;color: #ffffff"
     misc_feature    9233..9257
                     /label=RB T-DNA repeat
     primer_bind     complement(9261..9283)
                     /label=ZY065-RB
                     /note="ZY065-RB"
     CDS             10557..11186
                     /codon_start=1
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11620..12687
                     /codon_start=1
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      12753..12947
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    13291..13431
                     /label=bom
     rep_origin      complement(13617..14205)
                     /direction=LEFT
                     /label=ori
     CDS             complement(14295..15086)
                     /label=kanamycin
ORIGIN
        1 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
       61 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
      121 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
      181 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
      241 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
      301 tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
      361 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
      421 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
      481 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
      541 aatagagctt gtcgatcgtt aattaatcag atttcggtga cgggcaggac cggacggggc
      601 ggcaccggca ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg
      661 aagccggccg cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg
      721 ctcgggtcgt tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg
      781 gacttcagca ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggac
      841 acgtacacgg tggactcggc cgtccagtcg taggcgttgc gtgccttcca gggacccgcg
      901 taggcgatgc cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc
      961 agacggacga ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc
     1021 gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg
     1081 gcacggcgga tgtcggccgg gcgtcgttct gggctcatcc taggagagat agatttgtag
     1141 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
     1201 aaggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
     1261 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
     1321 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
     1381 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
     1441 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
     1501 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
     1561 agtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgaagacg tggttggaac
     1621 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     1681 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     1741 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     1801 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     1861 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgcacc tgcggctcta
     1921 gggagagtta acgaaaaatt taattattta aagtaagtat atttttcaat aacaatgcca
     1981 aaacctctaa tattcttaaa attaacaatt ggcaagtgat ttttaattag tttgctatta
     2041 ctattgttgt tattcttact actagcatta ttactattaa ttaattatta ttatcatcac
     2101 tatcattatt agtaaaaggt ttttttatgt ttgtatcttt taatttaata tttaaattaa
     2161 atacatttac atttattttg caatatttaa aaatgattag tttgtcaaat agtagagttc
     2221 atttaaaatt cttcagccat atagttctat ttttaagcta gtcgactttt ttttcttact
     2281 gaaaattaat atttttttct ttttgaaata ctaatacatc taaatttaac aattgccaaa
     2341 gtgattttta attagcttgc tggctaatca caataaaaat tactctcctt tactatataa
     2401 gtaaattttt attgctatat ttgttattat tattattatt attaatattt attttctaca
     2461 aatttaataa tattttattt tatatcattt taaaaagata agtaatgaaa tattaagaat
     2521 tcgtttataa ttcttttgca ggtgggtttc tatttgtaag ctaatctttt tcagttatcc
     2581 tttttttaaa atctttatta ttattatagc tatatctttt atcttttaaa attaacatta
     2641 tctattaaag ataatttcaa taaaagagta aaaattaatt tagagttcta ctgtcttcaa
     2701 atttctattt taaaaaatac ttttaaaact tgatgtattt tttacgtggt ttttcactat
     2761 gacttaattt ctgttttatt ataatatgta taaatataaa aatagatttt ccataacata
     2821 ttataaaaaa tgtaaggggc atttacgtaa atagatagac ttaaaagagg caccgagtga
     2881 accctaattc tcatcgttga gactataaaa tgcccattat cccattcgca cagtctcttc
     2941 attacttttg ctgttatttc tcctcagctg tgccgcatat cgcctaattt ttcttctcta
     3001 aggtttcatc atcttcacca atttctttaa tctcgattca attttttatg tttgatctgt
     3061 tattgttctg tcactacatg tgtttttcag ttgttttact agatgatttt cactgtcttc
     3121 ttgttagatc atacatatat tgaaaatgtt ttggattgac ttttttgtat tgtgaatatc
     3181 tgttattgtt tgattgttgt tcagtattta cacacccgat ctgtgttatg agcttggtca
     3241 taactatttc tctgtatgta aatacagatc tgttaatgtt tgtaatcaat ttttcatatg
     3301 cactgttgat attgttctct ctcctgtcct gttatatgtt gatatgattc ggtttttgta
     3361 taacttgaac taaacactag tcctaaatgt tttttttact atttaagatt tatataatat
     3421 ggatagattt tttgagttcc tagtctctga agaggttaag cttgctgtag ttgtttacca
     3481 gttgaggtgc aatactaaaa atcaattcaa ttactgatat tttttgctgt ttaggttttt
     3541 gacaaagtac tttaatttgc tttattgaac taaaaacgta gtcctgaatt cattgcaagt
     3601 gtgaaagcta tagttcattg tttttgttgc aattcttgaa aaattaattg gtcaagctat
     3661 aatggatttt actttttctg ttttaatatt gaatttgctg aatttatgaa tgggttgcat
     3721 ggtttttgaa atatgttgtt gtgtgttgtg taaaccatat gacaaagctg aaccagccat
     3781 acggcatcgg cctcgatatt ggctcaaact ctatcggctt cgcggtggtc gacgccaatt
     3841 cccacctcct gcgcctgaag ggcgagacag ccatcggcgc caggctcttc cgcgagggac
     3901 agtcagccgc ggatcggagg ggctctagga ccacacggag gcgcctctcc cggacaaggt
     3961 ggcgcctcag cttcctgcgc gacttcttcg cgccacatat caccaagatt gaccctgatt
     4021 tcttcctccg ccagaagtac tccgagatca gccctaagga caaggatcgg ttcaagtacg
     4081 agaagaggct gttcaacgac cgcaccgatg ccgagttcta cgaggattac ccatcaatgt
     4141 accacctgag gctccatctg atgacccaca cacataaggc cgacccgagg gagattttcc
     4201 tcgccatcca ccatattctc aagtctcggg gccacttcct cacacctgga gcggccaagg
     4261 atttcaatac cgacaaggtg gatctggagg acatcttccc agccctcaca gaggcgtacg
     4321 cccaggtcta cccggacctg gagctgacct tcgacctcgc gaaggccgac gatttcaagg
     4381 cgaagctcct ggatgagcag gccaccccgt ccgacacaca gaaggcgctg gtgaacctcc
     4441 tgctctccag cgatggcgag aaggagattg tcaagaagag gaagcaggtg ctgacagagt
     4501 tcgcgaaggc catcaccggc ctcaagacaa agttcaacct cgccctgggc accgaggtgg
     4561 atgaggccga tgcctcaaat tggcagttct ctatgggcca gctcgacgat aagtggtcca
     4621 acatcgagac atccatgaca gaccagggca ccgagatttt cgagcagatt caggagctgt
     4681 accgggcgag gctgctcaac ggcattgtgc cagccggcat gtccctgagc caggccaagg
     4741 tcgccgatta cggccagcac aaggaggacc tggagctgtt caagacatac ctcaagaagc
     4801 tgaatgatca tgagctggcg aagaccatcc ggggcctcta cgacaggtac attaacggcg
     4861 acgatgccaa gccgttcctg cgcgaggact tcgtcaaggc gctcaccaag gaggtgacag
     4921 cccatccaaa cgaggtgtcc gagcagctgc tcaacaggat gggccaggcc aatttcatgc
     4981 tcaagcagag gaccaaggcc aacggcgcaa tcccaattca gctccagcag cgcgagctgg
     5041 atcagatcat tgcgaaccag agcaagtact acgactggct cgccgcccca aatccagtgg
     5101 aggcacacag gtggaagatg ccgtaccagc tcgatgagct gctcaatttc catatcccat
     5161 actacgtcgg ccctctgatt acaccgaagc agcaggcgga gtcaggcgag aacgtgttcg
     5221 cctggatggt caggaaggac ccgtctggca acatcacccc atacaatttc gatgagaagg
     5281 tggaccggga ggcgtcagcc aatacattca tccagaggat gaagaccaca gatacctacc
     5341 tgattggcga ggacgtgctc cctaagcagt ctctgctcta ccagaagtac gaggtgctca
     5401 acgagctgaa caatgtccgc atcaacaatg agtgcctggg cacagatcag aagcagcggc
     5461 tcatcaggga ggtgttcgag cggcactcat ctgtgaccat taagcaggtc gcggataatc
     5521 tcgtggcaca tggcgacttc gcccggaggc cggagatcag gggcctggcg gacgagaaga
     5581 ggttcctctc cagcctgtcc acataccacc agctcaagga gatcctgcat gaggccattg
     5641 acgatccaac caagctgctc gatatcgaga acatcattac ctggagcaca gtgttcgagg
     5701 accacaccat cttcgagaca aagctggccg agattgagtg gctcgacccg aagaagatca
     5761 acgagctgtc aggcattcgc taccggggat ggggacagtt ctctcgcaag ctgctcgacg
     5821 gcctcaagct gggcaacggc cacacagtca ttcaggagct gatgctgagc aaccataatc
     5881 tcatgcagat cctggccgat gagacactca aggagacaat gacagagctg aaccaggaca
     5941 agctcaagac agacgatatc gaggatgtga ttaacgacgc gtacacctca ccatctaata
     6001 agaaggccct ccggcaggtc ctgagggtgg tcgaggatat taagcatgcc gcgaatggcc
     6061 aggacccttc atggctcttc atcgagacag ccgacggcac cggcacagcc ggcaagcgca
     6121 cacagtctcg gcagaagcag atccagaccg tgtacgcgaa cgccgcgcag gagctgattg
     6181 attccgccgt gaggggcgag ctggaggata agatcgcgga caaggcctct ttcacagacc
     6241 gcctcgtgct gtacttcatg cagggcggca gggatatcta cacaggcgcc cctctgaata
     6301 ttgaccagct ctcccactac gacatcgatc atattctccc gcagtcactg attaaggacg
     6361 attctctgga caacagggtg ctcgtcaacg cgacaatcaa tcgcgagaag aacaatgtgt
     6421 tcgcctccac actcttcgcg ggcaagatga aggcaacctg gcggaagtgg catgaggcag
     6481 gcctgatttc aggacgcaag ctccggaatc tcatgctgag gccggatgag atcgacaagt
     6541 tcgccaaggg attcgtggca aggcagctgg tggagacaag gcagatcatt aagctcacag
     6601 agcagatcgc tgccgcccag taccctaaca ccaagatcat tgccgtgaag gcgggcctca
     6661 gccaccagct gagggaggag ctggatttcc cgaagaacag ggacgtgaat cattaccacc
     6721 atgcgttcga tgccttcctc gccgcaagga ttggcacata cctgctcaag cgctacccga
     6781 agctggcgcc tttcttcacc tacggcgagt tcgccaaggt ggacgtgaag aagttcaggg
     6841 agttcaactt catcggcgcg ctcacacacg ccaagaagaa tatcattgcg aaggataccg
     6901 gcgagattgt gtgggataag gagagggaca tccgcgagct ggacaggatc tacaatttca
     6961 agcgcatgct cattacccat gaggtctact tcgagacagc cgatctcttc aagcagacca
     7021 tctacgccgc aaaggactcc aaggagaggg gcggcagcaa gcagctgatt cctaagaagc
     7081 agggctaccc gacacaggtg tacggcggat acacccagga gtcaggcagc tacaatgcgc
     7141 tcgtgcgcgt ggccgaggca gataccacag cctaccaggt catcaagatt tccgcgcaga
     7201 acgcctccaa gatcgcgagc gccaatctca agagcaggga gaagggcaag cagctgctca
     7261 atgagattgt ggtcaagcag ctggcgaagc gccggaagaa ctggaagcct tcagccaatt
     7321 ctttcaagat cgtgattccg aggttcggca tgggcacact gttccagaac gccaagtacg
     7381 gcctcttcat ggtcaactca gacacctact accgcaatta ccaggagctg tggctgtccc
     7441 gggagaatca gaagctgctc aagaagctct tcagcatcaa gtacgagaag acacagatga
     7501 accacgatgc cctccaggtg tacaaggcca tcattgacca ggtcgagaag ttcttcaagc
     7561 tgtacgatat caatcagttc agggcgaagc tctccgacgc cattgagcgc ttcgagaagc
     7621 tgccgatcaa caccgatggc aataagattg gcaagaccga gacactccgg cagatcctca
     7681 ttggcctcca ggcgaacggc acaaggtcca acgtgaagaa tctgggcatc aagaccgacc
     7741 tcggcctgct ccaggtcggc agcggcatca agctcgacaa ggatacccag attgtctacc
     7801 agtcaccgtc tggcctgttc aagaggcgca tcccactcgc cgacctgtca agggctgatc
     7861 ctaagaagaa gaggaaggtt tctagggcag atccaaagaa gaagcggaag gtgtctagag
     7921 ctgatcctaa gaagaagagg aaggtttgag gatctaaaaa aaaaaaaaaa aaaaaaaaaa
     7981 aaaaaaaaaa aaaaaaaaaa aaaaaaacaa agcaccagtg gtctagtggt agaatagtac
     8041 cctgccacgg tacagacccg ggttcgattc ccggctggtg caggagacct tatattcccc
     8101 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
     8161 cttccccgat aacggaaacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
     8221 catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg
     8281 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
     8341 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
     8401 cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
     8461 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
     8521 atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc
     8581 tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact
     8641 tcgggtatac atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata
     8701 ctgttatctg gcttggtctc agtctcaggt agatgtcaga tcaatcagaa atgattgatc
     8761 tgacatctac gagttgagat caaacaaagc ttcagctgag tttcaatttc tgagcccatg
     8821 ttgggccata catatgccac ccgagtgcaa atcgggtggc aacaaagcac cagtggtcta
     8881 gtggtagaat agtaccctgc cacggtacag acccgggttc gattcccggc tggtgcagga
     8941 tccatatgaa gatgaagatg aaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag
     9001 tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg gctttttcta atattaaatg
     9061 aatgtaagat cacattataa tgaataaaca aatgtttcta taatccattg tgaatgtttt
     9121 gttggatctc ttctgcagca tataactact gtatgtgcta tggtatggac tatggaatat
     9181 gattaaagat aagcgctgaa aagccgcagg tggagctcaa actatcagtg tttgacagga
     9241 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataacgga tatttaaaag
     9301 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     9361 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
     9421 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
     9481 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
     9541 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
     9601 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
     9661 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
     9721 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
     9781 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
     9841 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
     9901 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
     9961 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
    10021 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
    10081 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
    10141 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
    10201 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
    10261 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
    10321 atcgcggccg ggtacgtgtt cgagccgccc gcgcacggct caaccgtgcg gctgcatgaa
    10381 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
    10441 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
    10501 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
    10561 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
    10621 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
    10681 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
    10741 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
    10801 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
    10861 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
    10921 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
    10981 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
    11041 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
    11101 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
    11161 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
    11221 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
    11281 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
    11341 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
    11401 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
    11461 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
    11521 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca
    11581 atggcactgg aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg
    11641 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
    11701 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
    11761 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag
    11821 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
    11881 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
    11941 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
    12001 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
    12061 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
    12121 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
    12181 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
    12241 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
    12301 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
    12361 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
    12421 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
    12481 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
    12541 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
    12601 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
    12661 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
    12721 gcaggggaaa aaggtcgaaa aggcctcttt cctgtggata gcacgtacat tgggaaccca
    12781 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
    12841 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
    12901 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
    12961 cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc
    13021 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca
    13081 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat
    13141 caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    13201 cccggaaacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    13261 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
    13321 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    13381 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
    13441 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    13501 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    13561 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    13621 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    13681 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    13741 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    13801 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    13861 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    13921 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    13981 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    14041 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    14101 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    14161 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    14221 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    14281 cattctaggt actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca
    14341 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc
    14401 ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca
    14461 agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg
    14521 ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg
    14581 cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg
    14641 ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa
    14701 tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt
    14761 tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg
    14821 agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt
    14881 ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac
    14941 cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta
    15001 gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat
    15061 tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa
    15121 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
    15181 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
    15241 tagtat
//
