LOCUS       Exported               17506 bp DNA     circular SYN 12-JAN-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17506
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2029..6117
                     /codon_start=1
                     /label=LrCas9(D13A)
                     /label=LacCas9(D13A)
                     /translation="MTKLNQPYGIGLAIGSNSIGFAVVDANSHLLRLKGETAIGARLFR
                     EGQSAADRRGSRTTRRRLSRTRWRLSFLRDFFAPHITKIDPDFFLRQKYSEISPKDKDR
                     FKYEKRLFNDRTDAEFYEDYPSMYHLRLHLMTHTHKADPREIFLAIHHILKSRGHFLTP
                     GAAKDFNTDKVDLEDIFPALTEAYAQVYPDLELTFDLAKADDFKAKLLDEQATPSDTQK
                     ALVNLLLSSDGEKEIVKKRKQVLTEFAKAITGLKTKFNLALGTEVDEADASNWQFSMGQ
                     LDDKWSNIETSMTDQGTEIFEQIQELYRARLLNGIVPAGMSLSQAKVADYGQHKEDLEL
                     FKTYLKKLNDHELAKTIRGLYDRYINGDDAKPFLREDFVKALTKEVTAHPNEVSEQLLN
                     RMGQANFMLKQRTKANGAIPIQLQQRELDQIIANQSKYYDWLAAPNPVEAHRWKMPYQL
                     DELLNFHIPYYVGPLITPKQQAESGENVFAWMVRKDPSGNITPYNFDEKVDREASANTF
                     IQRMKTTDTYLIGEDVLPKQSLLYQKYEVLNELNNVRINNECLGTDQKQRLIREVFERH
                     SSVTIKQVADNLVAHGDFARRPEIRGLADEKRFLSSLSTYHQLKEILHEAIDDPTKLLD
                     IENIITWSTVFEDHTIFETKLAEIEWLDPKKINELSGIRYRGWGQFSRKLLDGLKLGNG
                     HTVIQELMLSNHNLMQILADETLKETMTELNQDKLKTDDIEDVINDAYTSPSNKKALRQ
                     VLRVVEDIKHAANGQDPSWLFIETADGTGTAGKRTQSRQKQIQTVYANAAQELIDSAVR
                     GELEDKIADKASFTDRLVLYFMQGGRDIYTGAPLNIDQLSHYDIDHILPQSLIKDDSLD
                     NRVLVNATINREKNNVFASTLFAGKMKATWRKWHEAGLISGRKLRNLMLRPDEIDKFAK
                     GFVARQLVETRQIIKLTEQIAAAQYPNTKIIAVKAGLSHQLREELDFPKNRDVNHYHHA
                     FDAFLAARIGTYLLKRYPKLAPFFTYGEFAKVDVKKFREFNFIGALTHAKKNIIAKDTG
                     EIVWDKERDIRELDRIYNFKRMLITHEVYFETADLFKQTIYAAKDSKERGGSKQLIPKK
                     QGYPTQVYGGYTQESGSYNALVRVAEADTTAYQVIKISAQNASKIASANLKSREKGKQL
                     LNEIVVKQLAKRRKNWKPSANSFKIVIPRFGMGTLFQNAKYGLFMVNSDTYYRNYQELW
                     LSRENQKLLKKLFSIKYEKTQMNHDALQVYKAIIDQVEKFFKLYDINQFRAKLSDAIER
                     FEKLPINTDGNKIGKTETLRQILIGLQANGTRSNVKNLGIKTDLGLLQVGSGIKLDKDT
                     QIVYQSPSGLFKRRIPLADL"
     misc_feature    2065..2067
                     /label=D13A
     CDS             6130..6150
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6358..6423
                     /codon_start=1
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6430..7023
                     /label=PmCDA1-Delta-NES
     CDS             7027..7047
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7054..7305
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7546..7566
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7579..7599
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7612..7632
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7642..7891
                     /label=HSP terminator
     promoter        7901..8263
                     /label=TaU6
     misc_feature    8265..8270
                     /label=BsaI
                     /note="BsaI"
     CDS             complement(8271..8576)
                     /codon_start=1
                     /label=ccdb
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     5'UTR           complement(8577..8895)
                     /label=ccdb 5' UTR
     misc_feature    8902..8907
                     /label=BsaI
                     /note="BsaI"
     misc_feature    8909..9047
                     /label=gRNA Scaffold
                     /label=LrCas9 gRNA Scaffold v2
     misc_feature    9113..9137
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    9132..9157
                     /label=T-Border
     primer_bind     9141..9163
                     /label=ZY065-RB
                     /note="ZY065-RB"
     CDS             10437..11066
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11500..12567
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      12633..12827
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    13171..13311
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(13497..14085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14172..14966)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    15291..15313
                     /label=NB462
     misc_feature    15391..15415
                     /label=T-Border
     primer_bind     15394..15416
                     /label=ZY065-LB
                     /note="ZY065-LB"
     polyA_signal    15493..15667
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     gene            complement(15693..16718)
                     /label=Hyg
     promoter        complement(16757..17434)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa tgagcattgc
       61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
      121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
      181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
      241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
      301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
      361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
      421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
      481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
      541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
      601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
      661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
      721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
      781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaactccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat gacaaagctg
     2041 aaccagccat acggcatcgg cctcgctatt ggctcaaact ctatcggctt cgcggtggtc
     2101 gacgccaatt cccacctcct gcgcctgaag ggcgagacag ccatcggcgc caggctcttc
     2161 cgcgagggac agtcagccgc ggatcggagg ggctctagga ccacacggag gcgcctctcc
     2221 cggacaaggt ggcgcctcag cttcctgcgc gacttcttcg cgccacatat caccaagatt
     2281 gaccctgatt tcttcctccg ccagaagtac tccgagatca gccctaagga caaggatcgg
     2341 ttcaagtacg agaagaggct gttcaacgac cgcaccgatg ccgagttcta cgaggattac
     2401 ccatcaatgt accacctgag gctccatctg atgacccaca cacataaggc cgacccgagg
     2461 gagattttcc tcgccatcca ccatattctc aagtctcggg gccacttcct cacacctgga
     2521 gcggccaagg atttcaatac cgacaaggtg gatctggagg acatcttccc agccctcaca
     2581 gaggcgtacg cccaggtcta cccggacctg gagctgacct tcgacctcgc gaaggccgac
     2641 gatttcaagg cgaagctcct ggatgagcag gccaccccgt ccgacacaca gaaggcgctg
     2701 gtgaacctcc tgctctccag cgatggcgag aaggagattg tcaagaagag gaagcaggtg
     2761 ctgacagagt tcgcgaaggc catcaccggc ctcaagacaa agttcaacct cgccctgggc
     2821 accgaggtgg atgaggccga tgcctcaaat tggcagttct ctatgggcca gctcgacgat
     2881 aagtggtcca acatcgagac atccatgaca gaccagggca ccgagatttt cgagcagatt
     2941 caggagctgt accgggcgag gctgctcaac ggcattgtgc cagccggcat gtccctgagc
     3001 caggccaagg tcgccgatta cggccagcac aaggaggacc tggagctgtt caagacatac
     3061 ctcaagaagc tgaatgatca tgagctggcg aagaccatcc ggggcctcta cgacaggtac
     3121 attaacggcg acgatgccaa gccgttcctg cgcgaggact tcgtcaaggc gctcaccaag
     3181 gaggtgacag cccatccaaa cgaggtgtcc gagcagctgc tcaacaggat gggccaggcc
     3241 aatttcatgc tcaagcagag gaccaaggcc aacggcgcaa tcccaattca gctccagcag
     3301 cgcgagctgg atcagatcat tgcgaaccag agcaagtact acgactggct cgccgcccca
     3361 aatccagtgg aggcacacag gtggaagatg ccgtaccagc tcgatgagct gctcaatttc
     3421 catatcccat actacgtcgg ccctctgatt acaccgaagc agcaggcgga gtcaggcgag
     3481 aacgtgttcg cctggatggt caggaaggac ccgtctggca acatcacccc atacaatttc
     3541 gatgagaagg tggaccggga ggcgtcagcc aatacattca tccagaggat gaagaccaca
     3601 gatacctacc tgattggcga ggacgtgctc cctaagcagt ctctgctcta ccagaagtac
     3661 gaggtgctca acgagctgaa caatgtccgc atcaacaatg agtgcctggg cacagatcag
     3721 aagcagcggc tcatcaggga ggtgttcgag cggcactcat ctgtgaccat taagcaggtc
     3781 gcggataatc tcgtggcaca tggcgacttc gcccggaggc cggagatcag gggcctggcg
     3841 gacgagaaga ggttcctctc cagcctgtcc acataccacc agctcaagga gatcctgcat
     3901 gaggccattg acgatccaac caagctgctc gatatcgaga acatcattac ctggagcaca
     3961 gtgttcgagg accacaccat cttcgagaca aagctggccg agattgagtg gctcgacccg
     4021 aagaagatca acgagctgtc aggcattcgc taccggggat ggggacagtt ctctcgcaag
     4081 ctgctcgacg gcctcaagct gggcaacggc cacacagtca ttcaggagct gatgctgagc
     4141 aaccataatc tcatgcagat cctggccgat gagacactca aggagacaat gacagagctg
     4201 aaccaggaca agctcaagac agacgatatc gaggatgtga ttaacgacgc gtacacctca
     4261 ccatctaata agaaggccct ccggcaggtc ctgagggtgg tcgaggatat taagcatgcc
     4321 gcgaatggcc aggacccttc atggctcttc atcgagacag ccgacggcac cggcacagcc
     4381 ggcaagcgca cacagtctcg gcagaagcag atccagaccg tgtacgcgaa cgccgcgcag
     4441 gagctgattg attccgccgt gaggggcgag ctggaggata agatcgcgga caaggcctct
     4501 ttcacagacc gcctcgtgct gtacttcatg cagggcggca gggatatcta cacaggcgcc
     4561 cctctgaata ttgaccagct ctcccactac gacatcgatc atattctccc gcagtcactg
     4621 attaaggacg attctctgga caacagggtg ctcgtcaacg cgacaatcaa tcgcgagaag
     4681 aacaatgtgt tcgcctccac actcttcgcg ggcaagatga aggcaacctg gcggaagtgg
     4741 catgaggcag gcctgatttc aggacgcaag ctccggaatc tcatgctgag gccggatgag
     4801 atcgacaagt tcgccaaggg attcgtggca aggcagctgg tggagacaag gcagatcatt
     4861 aagctcacag agcagatcgc tgccgcccag taccctaaca ccaagatcat tgccgtgaag
     4921 gcgggcctca gccaccagct gagggaggag ctggatttcc cgaagaacag ggacgtgaat
     4981 cattaccacc atgcgttcga tgccttcctc gccgcaagga ttggcacata cctgctcaag
     5041 cgctacccga agctggcgcc tttcttcacc tacggcgagt tcgccaaggt ggacgtgaag
     5101 aagttcaggg agttcaactt catcggcgcg ctcacacacg ccaagaagaa tatcattgcg
     5161 aaggataccg gcgagattgt gtgggataag gagagggaca tccgcgagct ggacaggatc
     5221 tacaatttca agcgcatgct cattacccat gaggtctact tcgagacagc cgatctcttc
     5281 aagcagacca tctacgccgc aaaggactcc aaggagaggg gcggcagcaa gcagctgatt
     5341 cctaagaagc agggctaccc gacacaggtg tacggcggat acacccagga gtcaggcagc
     5401 tacaatgcgc tcgtgcgcgt ggccgaggca gataccacag cctaccaggt catcaagatt
     5461 tccgcgcaga acgcctccaa gatcgcgagc gccaatctca agagcaggga gaagggcaag
     5521 cagctgctca atgagattgt ggtcaagcag ctggcgaagc gccggaagaa ctggaagcct
     5581 tcagccaatt ctttcaagat cgtgattccg aggttcggca tgggcacact gttccagaac
     5641 gccaagtacg gcctcttcat ggtcaactca gacacctact accgcaatta ccaggagctg
     5701 tggctgtccc gggagaatca gaagctgctc aagaagctct tcagcatcaa gtacgagaag
     5761 acacagatga accacgatgc cctccaggtg tacaaggcca tcattgacca ggtcgagaag
     5821 ttcttcaagc tgtacgatat caatcagttc agggcgaagc tctccgacgc cattgagcgc
     5881 ttcgagaagc tgccgatcaa caccgatggc aataagattg gcaagaccga gacactccgg
     5941 cagatcctca ttggcctcca ggcgaacggc acaaggtcca acgtgaagaa tctgggcatc
     6001 aagaccgacc tcggcctgct ccaggtcggc agcggcatca agctcgacaa ggatacccag
     6061 attgtctacc agtcaccgtc tggcctgttc aagaggcgca tcccactcgc cgacctgtca
     6121 agggctgatc ctaagaagaa gaggaaggtt ggaggtggcg gtacaggagg gggtgggtcc
     6181 gctgagtatg tcagggcgtt gttcgacttc aatggaaacg acgaggaaga tctgcctttt
     6241 aaaaagggag atattctcag gatcagagat aagccggaag aacaatggtg gaacgctgaa
     6301 gactctgaag gtaagagagg tatgattctt gtcccctacg tcgagaagta ttcgggtgac
     6361 tataaagacc acgatggaga ttataaggac cacgatatag attataagga tgatgatgat
     6421 aagagcggaa tgaccgatgc agagtacgtc aggattcatg agaaacttga catctacacg
     6481 tttaagaaac agtttttcaa caacaaaaaa tctgttagtc accgctgtta cgtgctgttc
     6541 gaattgaaac gcagaggtga gaggagagcc tgcttttggg gctatgccgt caacaagccg
     6601 caaagcggca cagaaagggg cattcacgcg gagatattta gcattagaaa ggtcgaggaa
     6661 taccttcggg ataatcccgg gcaattcact atcaattggt actcttcatg gtccccgtgt
     6721 gcagattgcg ctgaaaagat actggagtgg tataatcaag aactcagagg aaacggtcac
     6781 accctcaaga tttgggcttg caagctttac tacgagaaaa atgcaaggaa ccagatcggc
     6841 ctctggaact tgcgcgacaa cggcgtgggg ttgaatgtga tggtgtcgga gcattaccag
     6901 tgctgccgga agatattcat tcagtcgtca cataatcaat tgaacgagaa taggtggctc
     6961 gaaaaaaccc tgaagcgggc cgagaagtgg aggagtgaac tctcgataat gatccaggtt
     7021 aaaggaccga agaagaagcg caaggtgggg accatgacta atctctcaga tataatcgag
     7081 aaggaaacag gaaagcaact ggtcatccaa gaatcgattt tgatgcttcc cgaagaagtc
     7141 gaagaagtta taggaaataa gcccgagtct gacatactgg ttcacacagc gtacgatgaa
     7201 agtacggacg agaatgtcat gttgctgaca tcggacgcac ctgaatacaa gccttgggct
     7261 ctggtcatac aagatagtaa cggagaaaat aagattaaaa tgcttgaagg aaggggctcc
     7321 ctgctgacat gtggagatgt ggaagagaat ccaggaccat ccaggatgat agagcaagat
     7381 ggttgtaccc aagtgctgcg gcccctcgga tggcgggggt acagcgccat gactggccat
     7441 aatagacagt ccgctgctct catgcctcca tgcagcggat gtcagaggag gggagctagg
     7501 ttcttcctgt ctagacctac ctgccccgtg ccctcaaggg ctgatcctaa gaagaagagg
     7561 aaggtttcta gggcagatcc aaagaagaag cggaaggtgt ctagagctga tccgaagaag
     7621 aagcgcaagg tgtgactcga gatatgaaga tgaagatgaa atatttggtg tgtcaaataa
     7681 aaagcttgtg tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg aatttgtggc
     7741 tttttctaat attaaatgaa tgtaagatca cattataatg aataaacaaa tgtttctata
     7801 atccattgtg aatgttttgt tggatctctt ctgcagcata taactactgt atgtgctatg
     7861 gtatggacta tggaatatga ttaaagataa gaggatccgc gaccaagccc gttattctga
     7921 cagttctggt gctcaacaca tttatattta tcaaggagca cattgttact cactgctagg
     7981 agggaatcga actaggaata ttgatcagag gaactacgag agagctgaag ataactgccc
     8041 tctagctctc actgatctgg gtcgcatagt gagatgcagc ccacgtgagt tcagcaacgg
     8101 tctagcgctg ggcttttagg cccgcatgat cgggcttttg tcgggtggtc gacgtgttca
     8161 cgattgggga gagcaacgca gcagttcctc ttagtttagt cccacctcgc ctgtccagca
     8221 gagttctgac cggtttataa actcgcttgc tgcatcagac ttgggagacc ttatattccc
     8281 cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact
     8341 tcttccccga taacggaaac cggcacactg gccatatcgg tggtcatcat gcgccagctt
     8401 tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt
     8461 gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca
     8521 tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca
     8581 ccagcccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc
     8641 catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg
     8701 catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag caactgatag
     8761 ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac
     8821 ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat
     8881 actgttatct ggcttggtac cggtctcagt ctcaggtaga tgtcagatca atcagaaatg
     8941 attgatctga catctacgag ttgagatcaa acaaagcttc agctgagttt caatttctga
     9001 gcccatgttg ggccatacat atgccacccg agtgcaaatc gggtggcttt ttttctagac
     9061 ccagctttct tgtacaaagt tggcattact gcgagctcaa actatcagtg tttgacagga
     9121 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataacgga tatttaaaag
     9181 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     9241 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
     9301 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
     9361 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
     9421 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
     9481 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
     9541 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
     9601 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
     9661 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
     9721 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
     9781 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
     9841 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
     9901 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
     9961 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
    10021 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
    10081 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
    10141 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
    10201 atcgcggccg ggtacgtgtt cgagccgccc gcgcacggct caaccgtgcg gctgcatgaa
    10261 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
    10321 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
    10381 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
    10441 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
    10501 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
    10561 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
    10621 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
    10681 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
    10741 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
    10801 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
    10861 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
    10921 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
    10981 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
    11041 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
    11101 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
    11161 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
    11221 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
    11281 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
    11341 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
    11401 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca
    11461 atggcactgg aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg
    11521 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
    11581 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
    11641 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag
    11701 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
    11761 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
    11821 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
    11881 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
    11941 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
    12001 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
    12061 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
    12121 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
    12181 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
    12241 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
    12301 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
    12361 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
    12421 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
    12481 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
    12541 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
    12601 gcaggggaaa aaggtcgaaa aggcctcttt cctgtggata gcacgtacat tgggaaccca
    12661 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
    12721 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
    12781 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
    12841 cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc
    12901 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca
    12961 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat
    13021 caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    13081 cccggaaacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    13141 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
    13201 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    13261 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
    13321 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    13381 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    13441 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    13501 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    13561 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    13621 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    13681 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    13741 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    13801 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    13861 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    13921 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    13981 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    14041 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    14101 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    14161 cattctaggt actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca
    14221 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc
    14281 ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca
    14341 agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg
    14401 ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg
    14461 cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg
    14521 ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa
    14581 tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt
    14641 tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg
    14701 agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt
    14761 ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac
    14821 cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta
    14881 gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat
    14941 tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa
    15001 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
    15061 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
    15121 tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc
    15181 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
    15241 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
    15301 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
    15361 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt
    15421 gacgcttaga caacttaata acacattgcg gacgttttta atgtactgaa ttaacgccga
    15481 attaattcgg gggatctgga ttttagtact ggattttggt tttaggaatt agaaatttta
    15541 ttgatagaag tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca
    15601 tgagcgaaac cctataggaa ccctaattcc cttatctggg aactactcac acattattat
    15661 ggagaaaata gagcttgtcg atcgttaatt aatcacctcc aagatcccgg acgagtgctg
    15721 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
    15781 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
    15841 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
    15901 tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga
    15961 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
    16021 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
    16081 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
    16141 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
    16201 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
    16261 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
    16321 aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc
    16381 tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
    16441 cgggagatgc aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc
    16501 gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg
    16561 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
    16621 tcttcgccct ccgagagctg catcaggtcg gacacgctgt cgaacttttc gatcagaaac
    16681 ttctcgacag acgtcgcggt gagttcaggc tttttcatcc taggagagat agatttgtag
    16741 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
    16801 aaggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
    16861 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
    16921 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
    16981 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
    17041 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
    17101 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
    17161 agtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgaagacg tggttggaac
    17221 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
    17281 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
    17341 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
    17401 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
    17461 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatg
//
