LOCUS       Exported               16569 bp DNA     circular SYN 12-JAN-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     LOCUS       pTC97                  13319 bp    DNA     circular UNA 
            19-AUG-2013
            DEFINITION  Hyg selection, 35S:TALEN:P2A:TALEN:HSP terminator GG 
            compatible
                        cloning vector, ELD, KKR FokI heterodimers, pCAMBIA 
            backbone.
            ACCESSION   urn.local...1379278031277.6395
            VERSION     urn.local...1379278031277.6395
            KEYWORDS    .
            SOURCE
              ORGANISM
                        .
FEATURES             Location/Qualifiers
     source          1..16569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          7334..7958
                     /label=2012-07-10-pZHY738
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2029..2529
                     /codon_start=1
                     /label=ecTadA8e
                     /translation="MSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGW
                     NRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVF
                     GVRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQ
                     SSIN"
     misc_feature    2353..2355
                     /label=A109S
     misc_feature    2359..2361
                     /label=T111R
     misc_feature    2383..2385
                     /label=D119N
     misc_feature    2392..2394
                     /label=H122N
     misc_feature    2467..2469
                     /label=Y147D
     misc_feature    2473..2475
                     /label=F149D
     misc_feature    2524..2526
                     /label=T166I
     misc_feature    2527..2529
                     /label=D167N
     misc_feature    2530..2625
                     /label=32 Aa linker
     CDS             2626..6714
                     /codon_start=1
                     /label=LrCas9(D13A)
                     /label=LacCas9(D13A)
                     /translation="MTKLNQPYGIGLAIGSNSIGFAVVDANSHLLRLKGETAIGARLFR
                     EGQSAADRRGSRTTRRRLSRTRWRLSFLRDFFAPHITKIDPDFFLRQKYSEISPKDKDR
                     FKYEKRLFNDRTDAEFYEDYPSMYHLRLHLMTHTHKADPREIFLAIHHILKSRGHFLTP
                     GAAKDFNTDKVDLEDIFPALTEAYAQVYPDLELTFDLAKADDFKAKLLDEQATPSDTQK
                     ALVNLLLSSDGEKEIVKKRKQVLTEFAKAITGLKTKFNLALGTEVDEADASNWQFSMGQ
                     LDDKWSNIETSMTDQGTEIFEQIQELYRARLLNGIVPAGMSLSQAKVADYGQHKEDLEL
                     FKTYLKKLNDHELAKTIRGLYDRYINGDDAKPFLREDFVKALTKEVTAHPNEVSEQLLN
                     RMGQANFMLKQRTKANGAIPIQLQQRELDQIIANQSKYYDWLAAPNPVEAHRWKMPYQL
                     DELLNFHIPYYVGPLITPKQQAESGENVFAWMVRKDPSGNITPYNFDEKVDREASANTF
                     IQRMKTTDTYLIGEDVLPKQSLLYQKYEVLNELNNVRINNECLGTDQKQRLIREVFERH
                     SSVTIKQVADNLVAHGDFARRPEIRGLADEKRFLSSLSTYHQLKEILHEAIDDPTKLLD
                     IENIITWSTVFEDHTIFETKLAEIEWLDPKKINELSGIRYRGWGQFSRKLLDGLKLGNG
                     HTVIQELMLSNHNLMQILADETLKETMTELNQDKLKTDDIEDVINDAYTSPSNKKALRQ
                     VLRVVEDIKHAANGQDPSWLFIETADGTGTAGKRTQSRQKQIQTVYANAAQELIDSAVR
                     GELEDKIADKASFTDRLVLYFMQGGRDIYTGAPLNIDQLSHYDIDHILPQSLIKDDSLD
                     NRVLVNATINREKNNVFASTLFAGKMKATWRKWHEAGLISGRKLRNLMLRPDEIDKFAK
                     GFVARQLVETRQIIKLTEQIAAAQYPNTKIIAVKAGLSHQLREELDFPKNRDVNHYHHA
                     FDAFLAARIGTYLLKRYPKLAPFFTYGEFAKVDVKKFREFNFIGALTHAKKNIIAKDTG
                     EIVWDKERDIRELDRIYNFKRMLITHEVYFETADLFKQTIYAAKDSKERGGSKQLIPKK
                     QGYPTQVYGGYTQESGSYNALVRVAEADTTAYQVIKISAQNASKIASANLKSREKGKQL
                     LNEIVVKQLAKRRKNWKPSANSFKIVIPRFGMGTLFQNAKYGLFMVNSDTYYRNYQELW
                     LSRENQKLLKKLFSIKYEKTQMNHDALQVYKAIIDQVEKFFKLYDINQFRAKLSDAIER
                     FEKLPINTDGNKIGKTETLRQILIGLQANGTRSNVKNLGIKTDLGLLQVGSGIKLDKDT
                     QIVYQSPSGLFKRRIPLADL"
     misc_feature    2662..2664
                     /label=D13A
     CDS             6727..6747
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6760..6780
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6793..6813
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6823..7072
                     /label=HSP terminator
     misc_feature    7082..7327
                     /label=Rice U6-2
                     /note="2013-Cell Research gRNA-Zhu"
     CDS             complement(7334..7639)
                     /codon_start=1
                     /label=ccdb
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     5'UTR           complement(7640..7958)
                     /label=ccdb 5' UTR
     misc_feature    7972..8110
                     /label=gRNA Scaffold
                     /label=LrCas9 gRNA Scaffold v2
     misc_feature    8176..8200
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    8195..8220
                     /label=T-Border
     primer_bind     8204..8226
                     /label=ZY065-RB
                     /note="ZY065-RB"
     CDS             9500..10129
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10563..11630
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      11696..11890
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    12234..12374
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(12560..13148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13235..14029)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    14454..14478
                     /label=T-Border
     primer_bind     14457..14479
                     /label=ZY065-LB
                     /note="ZY065-LB"
     polyA_signal    14556..14730
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     gene            complement(14756..15781)
                     /label=Hyg
     promoter        complement(15820..16497)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa tgagcattgc
       61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
      121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
      181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
      241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
      301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
      361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
      421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
      481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
      541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
      601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
      661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
      721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
      781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaactccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat gtctgaggtg
     2041 gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag ggcacgggat
     2101 gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat cggcgagggc
     2161 tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat ggccctgaga
     2221 cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta cgtgacattc
     2281 gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg cgtggtgttt
     2341 ggcgtgagga actcaaaaag aggcgccgca ggctccctga tgaacgtgct gaactacccc
     2401 ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg tgccgccctg
     2461 ctgtgcgatt tctatcggat gcctagacag gtgttcaatg ctcagaagaa ggcccagagc
     2521 tccatcaact ccggaggatc tagcggaggc tcctctggct ctgagacacc tggcacaagc
     2581 gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcaatgac aaagctgaac
     2641 cagccatacg gcatcggcct cgctattggc tcaaactcta tcggcttcgc ggtggtcgac
     2701 gccaattccc acctcctgcg cctgaagggc gagacagcca tcggcgccag gctcttccgc
     2761 gagggacagt cagccgcgga tcggaggggc tctaggacca cacggaggcg cctctcccgg
     2821 acaaggtggc gcctcagctt cctgcgcgac ttcttcgcgc cacatatcac caagattgac
     2881 cctgatttct tcctccgcca gaagtactcc gagatcagcc ctaaggacaa ggatcggttc
     2941 aagtacgaga agaggctgtt caacgaccgc accgatgccg agttctacga ggattaccca
     3001 tcaatgtacc acctgaggct ccatctgatg acccacacac ataaggccga cccgagggag
     3061 attttcctcg ccatccacca tattctcaag tctcggggcc acttcctcac acctggagcg
     3121 gccaaggatt tcaataccga caaggtggat ctggaggaca tcttcccagc cctcacagag
     3181 gcgtacgccc aggtctaccc ggacctggag ctgaccttcg acctcgcgaa ggccgacgat
     3241 ttcaaggcga agctcctgga tgagcaggcc accccgtccg acacacagaa ggcgctggtg
     3301 aacctcctgc tctccagcga tggcgagaag gagattgtca agaagaggaa gcaggtgctg
     3361 acagagttcg cgaaggccat caccggcctc aagacaaagt tcaacctcgc cctgggcacc
     3421 gaggtggatg aggccgatgc ctcaaattgg cagttctcta tgggccagct cgacgataag
     3481 tggtccaaca tcgagacatc catgacagac cagggcaccg agattttcga gcagattcag
     3541 gagctgtacc gggcgaggct gctcaacggc attgtgccag ccggcatgtc cctgagccag
     3601 gccaaggtcg ccgattacgg ccagcacaag gaggacctgg agctgttcaa gacatacctc
     3661 aagaagctga atgatcatga gctggcgaag accatccggg gcctctacga caggtacatt
     3721 aacggcgacg atgccaagcc gttcctgcgc gaggacttcg tcaaggcgct caccaaggag
     3781 gtgacagccc atccaaacga ggtgtccgag cagctgctca acaggatggg ccaggccaat
     3841 ttcatgctca agcagaggac caaggccaac ggcgcaatcc caattcagct ccagcagcgc
     3901 gagctggatc agatcattgc gaaccagagc aagtactacg actggctcgc cgccccaaat
     3961 ccagtggagg cacacaggtg gaagatgccg taccagctcg atgagctgct caatttccat
     4021 atcccatact acgtcggccc tctgattaca ccgaagcagc aggcggagtc aggcgagaac
     4081 gtgttcgcct ggatggtcag gaaggacccg tctggcaaca tcaccccata caatttcgat
     4141 gagaaggtgg accgggaggc gtcagccaat acattcatcc agaggatgaa gaccacagat
     4201 acctacctga ttggcgagga cgtgctccct aagcagtctc tgctctacca gaagtacgag
     4261 gtgctcaacg agctgaacaa tgtccgcatc aacaatgagt gcctgggcac agatcagaag
     4321 cagcggctca tcagggaggt gttcgagcgg cactcatctg tgaccattaa gcaggtcgcg
     4381 gataatctcg tggcacatgg cgacttcgcc cggaggccgg agatcagggg cctggcggac
     4441 gagaagaggt tcctctccag cctgtccaca taccaccagc tcaaggagat cctgcatgag
     4501 gccattgacg atccaaccaa gctgctcgat atcgagaaca tcattacctg gagcacagtg
     4561 ttcgaggacc acaccatctt cgagacaaag ctggccgaga ttgagtggct cgacccgaag
     4621 aagatcaacg agctgtcagg cattcgctac cggggatggg gacagttctc tcgcaagctg
     4681 ctcgacggcc tcaagctggg caacggccac acagtcattc aggagctgat gctgagcaac
     4741 cataatctca tgcagatcct ggccgatgag acactcaagg agacaatgac agagctgaac
     4801 caggacaagc tcaagacaga cgatatcgag gatgtgatta acgacgcgta cacctcacca
     4861 tctaataaga aggccctccg gcaggtcctg agggtggtcg aggatattaa gcatgccgcg
     4921 aatggccagg acccttcatg gctcttcatc gagacagccg acggcaccgg cacagccggc
     4981 aagcgcacac agtctcggca gaagcagatc cagaccgtgt acgcgaacgc cgcgcaggag
     5041 ctgattgatt ccgccgtgag gggcgagctg gaggataaga tcgcggacaa ggcctctttc
     5101 acagaccgcc tcgtgctgta cttcatgcag ggcggcaggg atatctacac aggcgcccct
     5161 ctgaatattg accagctctc ccactacgac atcgatcata ttctcccgca gtcactgatt
     5221 aaggacgatt ctctggacaa cagggtgctc gtcaacgcga caatcaatcg cgagaagaac
     5281 aatgtgttcg cctccacact cttcgcgggc aagatgaagg caacctggcg gaagtggcat
     5341 gaggcaggcc tgatttcagg acgcaagctc cggaatctca tgctgaggcc ggatgagatc
     5401 gacaagttcg ccaagggatt cgtggcaagg cagctggtgg agacaaggca gatcattaag
     5461 ctcacagagc agatcgctgc cgcccagtac cctaacacca agatcattgc cgtgaaggcg
     5521 ggcctcagcc accagctgag ggaggagctg gatttcccga agaacaggga cgtgaatcat
     5581 taccaccatg cgttcgatgc cttcctcgcc gcaaggattg gcacatacct gctcaagcgc
     5641 tacccgaagc tggcgccttt cttcacctac ggcgagttcg ccaaggtgga cgtgaagaag
     5701 ttcagggagt tcaacttcat cggcgcgctc acacacgcca agaagaatat cattgcgaag
     5761 gataccggcg agattgtgtg ggataaggag agggacatcc gcgagctgga caggatctac
     5821 aatttcaagc gcatgctcat tacccatgag gtctacttcg agacagccga tctcttcaag
     5881 cagaccatct acgccgcaaa ggactccaag gagaggggcg gcagcaagca gctgattcct
     5941 aagaagcagg gctacccgac acaggtgtac ggcggataca cccaggagtc aggcagctac
     6001 aatgcgctcg tgcgcgtggc cgaggcagat accacagcct accaggtcat caagatttcc
     6061 gcgcagaacg cctccaagat cgcgagcgcc aatctcaaga gcagggagaa gggcaagcag
     6121 ctgctcaatg agattgtggt caagcagctg gcgaagcgcc ggaagaactg gaagccttca
     6181 gccaattctt tcaagatcgt gattccgagg ttcggcatgg gcacactgtt ccagaacgcc
     6241 aagtacggcc tcttcatggt caactcagac acctactacc gcaattacca ggagctgtgg
     6301 ctgtcccggg agaatcagaa gctgctcaag aagctcttca gcatcaagta cgagaagaca
     6361 cagatgaacc acgatgccct ccaggtgtac aaggccatca ttgaccaggt cgagaagttc
     6421 ttcaagctgt acgatatcaa tcagttcagg gcgaagctct ccgacgccat tgagcgcttc
     6481 gagaagctgc cgatcaacac cgatggcaat aagattggca agaccgagac actccggcag
     6541 atcctcattg gcctccaggc gaacggcaca aggtccaacg tgaagaatct gggcatcaag
     6601 accgacctcg gcctgctcca ggtcggcagc ggcatcaagc tcgacaagga tacccagatt
     6661 gtctaccagt caccgtctgg cctgttcaag aggcgcatcc cactcgccga cctgtcaagg
     6721 gctgatccta agaagaagag gaaggtttct agggcagatc caaagaagaa gcggaaggtg
     6781 tctagagctg atccgaagaa gaagcgcaag gtgtgactcg agatatgaag atgaagatga
     6841 aatatttggt gtgtcaaata aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt
     6901 gttgtgttat gaatttgtgg ctttttctaa tattaaatga atgtaagatc acattataat
     6961 gaataaacaa atgtttctat aatccattgt gaatgttttg ttggatctct tctgcagcat
     7021 ataactactg tatgtgctat ggtatggact atggaatatg attaaagata agaggatccg
     7081 cggatcatga accaacggcc tggctgtatt tggtggttgt gtagggagat ggggagaaga
     7141 aaagcccgat tctcttcgct gtgatgggct ggatgcatgc gggggagcgg gaggcccaag
     7201 tacgtgcacg gtgagcggcc cacagggcga gtgtgagcgc gagaggcggg aggaacagtt
     7261 tagtaccaca ttgcccagct aactcgaacg cgaccaactt ataaacccgc gcgctgtcgc
     7321 ttgtgtggag accttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt
     7381 cgcggtggct gagatcagcc acttcttccc cgataacgga aaccggcaca ctggccatat
     7441 cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg
     7501 agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg
     7561 tgtcaataat atcactctgt acatccacaa acagacgata acggctctct cttttatagg
     7621 tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt
     7681 tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca
     7741 cgcagacgac gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca
     7801 tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca
     7861 tagcatacct ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca
     7921 aaaatcagcg cgcaaatacg catactgtta tctggcttgg taccggtctc agtctcaggt
     7981 agatgtcaga tcaatcagaa atgattgatc tgacatctac gagttgagat caaacaaagc
     8041 ttcagctgag tttcaatttc tgagcccatg ttgggccata catatgccac ccgagtgcaa
     8101 atcgggtggc tttttttcta gacccagctt tcttgtacaa agttggcatt actgcgagct
     8161 caaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
     8221 ttagaataac ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     8281 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc
     8341 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
     8401 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
     8461 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
     8521 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
     8581 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
     8641 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
     8701 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
     8761 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
     8821 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
     8881 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
     8941 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
     9001 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
     9061 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
     9121 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
     9181 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
     9241 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
     9301 gctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
     9361 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
     9421 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
     9481 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
     9541 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
     9601 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
     9661 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
     9721 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
     9781 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
     9841 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
     9901 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
     9961 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
    10021 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
    10081 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
    10141 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
    10201 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
    10261 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
    10321 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
    10381 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
    10441 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
    10501 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
    10561 acggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
    10621 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
    10681 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
    10741 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
    10801 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
    10861 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
    10921 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
    10981 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
    11041 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
    11101 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
    11161 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
    11221 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
    11281 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
    11341 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
    11401 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
    11461 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
    11521 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
    11581 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
    11641 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggcctc tttcctgtgg
    11701 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
    11761 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
    11821 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
    11881 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
    11941 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
    12001 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
    12061 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
    12121 gaaaacctct gacacatgca gctcccggaa acggtcacag cttgtctgta agcggatgcc
    12181 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
    12241 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
    12301 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    12361 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    12421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    12481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    12541 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    12601 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    12661 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    12721 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    12781 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    12841 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    12901 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    12961 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    13021 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    13081 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    13141 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    13201 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat
    13261 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
    13321 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
    13381 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
    13441 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
    13501 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
    13561 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
    13621 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
    13681 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
    13741 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
    13801 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
    13861 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
    13921 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
    13981 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
    14041 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
    14101 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
    14161 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
    14221 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
    14281 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
    14341 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
    14401 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
    14461 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
    14521 ttaatgtact gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt
    14581 ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa
    14641 gggtttctta tatgctcaac acatgagcga aaccctatag gaaccctaat tcccttatct
    14701 gggaactact cacacattat tatggagaaa atagagcttg tcgatcgtta attaatcacc
    14761 tccaagatcc cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac
    14821 agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg
    14881 ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc
    14941 cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc
    15001 gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac
    15061 aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca
    15121 tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg
    15181 agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca
    15241 gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt
    15301 gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga
    15361 ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg
    15421 catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt
    15481 gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaattccc
    15541 caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac
    15601 gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta
    15661 catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggacacgc
    15721 tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca
    15781 tcctaggaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg
    15841 aaatgaactt ccttatatag aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc
    15901 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
    15961 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
    16021 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
    16081 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
    16141 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
    16201 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttatcaca tcaatccact
    16261 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
    16321 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
    16381 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
    16441 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
    16501 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
    16561 tccaccatg
//
