LOCUS       Exported               17364 bp DNA     circular SYN 12-JAN-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          8129..8753
                     /label=2012-07-10-pZHY738
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2029..6117
                     /codon_start=1
                     /label=LrCas9
                     /label=LacCas9
                     /translation="MTKLNQPYGIGLDIGSNSIGFAVVDANSHLLRLKGETAIGARLFR
                     EGQSAADRRGSRTTRRRLSRTRWRLSFLRDFFAPHITKIDPDFFLRQKYSEISPKDKDR
                     FKYEKRLFNDRTDAEFYEDYPSMYHLRLHLMTHTHKADPREIFLAIHHILKSRGHFLTP
                     GAAKDFNTDKVDLEDIFPALTEAYAQVYPDLELTFDLAKADDFKAKLLDEQATPSDTQK
                     ALVNLLLSSDGEKEIVKKRKQVLTEFAKAITGLKTKFNLALGTEVDEADASNWQFSMGQ
                     LDDKWSNIETSMTDQGTEIFEQIQELYRARLLNGIVPAGMSLSQAKVADYGQHKEDLEL
                     FKTYLKKLNDHELAKTIRGLYDRYINGDDAKPFLREDFVKALTKEVTAHPNEVSEQLLN
                     RMGQANFMLKQRTKANGAIPIQLQQRELDQIIANQSKYYDWLAAPNPVEAHRWKMPYQL
                     DELLNFHIPYYVGPLITPKQQAESGENVFAWMVRKDPSGNITPYNFDEKVDREASANTF
                     IQRMKTTDTYLIGEDVLPKQSLLYQKYEVLNELNNVRINNECLGTDQKQRLIREVFERH
                     SSVTIKQVADNLVAHGDFARRPEIRGLADEKRFLSSLSTYHQLKEILHEAIDDPTKLLD
                     IENIITWSTVFEDHTIFETKLAEIEWLDPKKINELSGIRYRGWGQFSRKLLDGLKLGNG
                     HTVIQELMLSNHNLMQILADETLKETMTELNQDKLKTDDIEDVINDAYTSPSNKKALRQ
                     VLRVVEDIKHAANGQDPSWLFIETADGTGTAGKRTQSRQKQIQTVYANAAQELIDSAVR
                     GELEDKIADKASFTDRLVLYFMQGGRDIYTGAPLNIDQLSHYDIDHILPQSLIKDDSLD
                     NRVLVNATINREKNNVFASTLFAGKMKATWRKWHEAGLISGRKLRNLMLRPDEIDKFAK
                     GFVARQLVETRQIIKLTEQIAAAQYPNTKIIAVKAGLSHQLREELDFPKNRDVNHYHHA
                     FDAFLAARIGTYLLKRYPKLAPFFTYGEFAKVDVKKFREFNFIGALTHAKKNIIAKDTG
                     EIVWDKERDIRELDRIYNFKRMLITHEVYFETADLFKQTIYAAKDSKERGGSKQLIPKK
                     QGYPTQVYGGYTQESGSYNALVRVAEADTTAYQVIKISAQNASKIASANLKSREKGKQL
                     LNEIVVKQLAKRRKNWKPSANSFKIVIPRFGMGTLFQNAKYGLFMVNSDTYYRNYQELW
                     LSRENQKLLKKLFSIKYEKTQMNHDALQVYKAIIDQVEKFFKLYDINQFRAKLSDAIER
                     FEKLPINTDGNKIGKTETLRQILIGLQANGTRSNVKNLGIKTDLGLLQVGSGIKLDKDT
                     QIVYQSPSGLFKRRIPLADL"
     CDS             6148..6168
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6181..6201
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6214..6234
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6256..6408
                     /label=TAL
     misc_feature    6424..6576
                     /label=TAL
     misc_feature    6598..6750
                     /label=TAL
     misc_feature    6766..6918
                     /label=TAL
     misc_feature    6940..7092
                     /label=TAL
     misc_feature    7108..7260
                     /label=TAL
     misc_feature    7297..7446
                     /label=VP64
     misc_feature    7459..7608
                     /label=VP64
     terminator      7618..7867
                     /label=HSP terminator
     misc_feature    7877..8122
                     /label=Rice U6-2
                     /note="2013-Cell Research gRNA-Zhu"
     misc_feature    8123..8128
                     /label=BsaI
                     /note="BsaI"
     CDS             complement(8129..8434)
                     /codon_start=1
                     /label=ccdb
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     5'UTR           complement(8435..8753)
                     /label=ccdb 5' UTR
     misc_feature    8760..8765
                     /label=BsaI
                     /note="BsaI"
     misc_feature    8767..8905
                     /label=LrCas9 gRNA Scaffold v2
     misc_feature    8906..8950
                     /label=from pPAA033
                     /note="from pPAA033"
     misc_feature    8971..8995
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    8990..9015
                     /label=T-Border
     CDS             10295..10924
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11358..12425
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     modified_base   12482
                     /label=User
     rep_origin      12491..12685
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    13029..13169
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(13355..13943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14030..14824)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    15249..15273
                     /label=T-Border
     polyA_signal    15351..15525
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     gene            complement(15551..16576)
                     /label=Hyg
     promoter        complement(16615..17292)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa tgagcattgc
       61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
      121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
      181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
      241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
      301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
      361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
      421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
      481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
      541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
      601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
      661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
      721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
      781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaactccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat gacaaagctg
     2041 aaccagccat acggcatcgg cctcgatatt ggctcaaact ctatcggctt cgcggtggtc
     2101 gacgccaatt cccacctcct gcgcctgaag ggcgagacag ccatcggcgc caggctcttc
     2161 cgcgagggac agtcagccgc ggatcggagg ggctctagga ccacacggag gcgcctctcc
     2221 cggacaaggt ggcgcctcag cttcctgcgc gacttcttcg cgccacatat caccaagatt
     2281 gaccctgatt tcttcctccg ccagaagtac tccgagatca gccctaagga caaggatcgg
     2341 ttcaagtacg agaagaggct gttcaacgac cgcaccgatg ccgagttcta cgaggattac
     2401 ccatcaatgt accacctgag gctccatctg atgacccaca cacataaggc cgacccgagg
     2461 gagattttcc tcgccatcca ccatattctc aagtctcggg gccacttcct cacacctgga
     2521 gcggccaagg atttcaatac cgacaaggtg gatctggagg acatcttccc agccctcaca
     2581 gaggcgtacg cccaggtcta cccggacctg gagctgacct tcgacctcgc gaaggccgac
     2641 gatttcaagg cgaagctcct ggatgagcag gccaccccgt ccgacacaca gaaggcgctg
     2701 gtgaacctcc tgctctccag cgatggcgag aaggagattg tcaagaagag gaagcaggtg
     2761 ctgacagagt tcgcgaaggc catcaccggc ctcaagacaa agttcaacct cgccctgggc
     2821 accgaggtgg atgaggccga tgcctcaaat tggcagttct ctatgggcca gctcgacgat
     2881 aagtggtcca acatcgagac atccatgaca gaccagggca ccgagatttt cgagcagatt
     2941 caggagctgt accgggcgag gctgctcaac ggcattgtgc cagccggcat gtccctgagc
     3001 caggccaagg tcgccgatta cggccagcac aaggaggacc tggagctgtt caagacatac
     3061 ctcaagaagc tgaatgatca tgagctggcg aagaccatcc ggggcctcta cgacaggtac
     3121 attaacggcg acgatgccaa gccgttcctg cgcgaggact tcgtcaaggc gctcaccaag
     3181 gaggtgacag cccatccaaa cgaggtgtcc gagcagctgc tcaacaggat gggccaggcc
     3241 aatttcatgc tcaagcagag gaccaaggcc aacggcgcaa tcccaattca gctccagcag
     3301 cgcgagctgg atcagatcat tgcgaaccag agcaagtact acgactggct cgccgcccca
     3361 aatccagtgg aggcacacag gtggaagatg ccgtaccagc tcgatgagct gctcaatttc
     3421 catatcccat actacgtcgg ccctctgatt acaccgaagc agcaggcgga gtcaggcgag
     3481 aacgtgttcg cctggatggt caggaaggac ccgtctggca acatcacccc atacaatttc
     3541 gatgagaagg tggaccggga ggcgtcagcc aatacattca tccagaggat gaagaccaca
     3601 gatacctacc tgattggcga ggacgtgctc cctaagcagt ctctgctcta ccagaagtac
     3661 gaggtgctca acgagctgaa caatgtccgc atcaacaatg agtgcctggg cacagatcag
     3721 aagcagcggc tcatcaggga ggtgttcgag cggcactcat ctgtgaccat taagcaggtc
     3781 gcggataatc tcgtggcaca tggcgacttc gcccggaggc cggagatcag gggcctggcg
     3841 gacgagaaga ggttcctctc cagcctgtcc acataccacc agctcaagga gatcctgcat
     3901 gaggccattg acgatccaac caagctgctc gatatcgaga acatcattac ctggagcaca
     3961 gtgttcgagg accacaccat cttcgagaca aagctggccg agattgagtg gctcgacccg
     4021 aagaagatca acgagctgtc aggcattcgc taccggggat ggggacagtt ctctcgcaag
     4081 ctgctcgacg gcctcaagct gggcaacggc cacacagtca ttcaggagct gatgctgagc
     4141 aaccataatc tcatgcagat cctggccgat gagacactca aggagacaat gacagagctg
     4201 aaccaggaca agctcaagac agacgatatc gaggatgtga ttaacgacgc gtacacctca
     4261 ccatctaata agaaggccct ccggcaggtc ctgagggtgg tcgaggatat taagcatgcc
     4321 gcgaatggcc aggacccttc atggctcttc atcgagacag ccgacggcac cggcacagcc
     4381 ggcaagcgca cacagtctcg gcagaagcag atccagaccg tgtacgcgaa cgccgcgcag
     4441 gagctgattg attccgccgt gaggggcgag ctggaggata agatcgcgga caaggcctct
     4501 ttcacagacc gcctcgtgct gtacttcatg cagggcggca gggatatcta cacaggcgcc
     4561 cctctgaata ttgaccagct ctcccactac gacatcgatc atattctccc gcagtcactg
     4621 attaaggacg attctctgga caacagggtg ctcgtcaacg cgacaatcaa tcgcgagaag
     4681 aacaatgtgt tcgcctccac actcttcgcg ggcaagatga aggcaacctg gcggaagtgg
     4741 catgaggcag gcctgatttc aggacgcaag ctccggaatc tcatgctgag gccggatgag
     4801 atcgacaagt tcgccaaggg attcgtggca aggcagctgg tggagacaag gcagatcatt
     4861 aagctcacag agcagatcgc tgccgcccag taccctaaca ccaagatcat tgccgtgaag
     4921 gcgggcctca gccaccagct gagggaggag ctggatttcc cgaagaacag ggacgtgaat
     4981 cattaccacc atgcgttcga tgccttcctc gccgcaagga ttggcacata cctgctcaag
     5041 cgctacccga agctggcgcc tttcttcacc tacggcgagt tcgccaaggt ggacgtgaag
     5101 aagttcaggg agttcaactt catcggcgcg ctcacacacg ccaagaagaa tatcattgcg
     5161 aaggataccg gcgagattgt gtgggataag gagagggaca tccgcgagct ggacaggatc
     5221 tacaatttca agcgcatgct cattacccat gaggtctact tcgagacagc cgatctcttc
     5281 aagcagacca tctacgccgc aaaggactcc aaggagaggg gcggcagcaa gcagctgatt
     5341 cctaagaagc agggctaccc gacacaggtg tacggcggat acacccagga gtcaggcagc
     5401 tacaatgcgc tcgtgcgcgt ggccgaggca gataccacag cctaccaggt catcaagatt
     5461 tccgcgcaga acgcctccaa gatcgcgagc gccaatctca agagcaggga gaagggcaag
     5521 cagctgctca atgagattgt ggtcaagcag ctggcgaagc gccggaagaa ctggaagcct
     5581 tcagccaatt ctttcaagat cgtgattccg aggttcggca tgggcacact gttccagaac
     5641 gccaagtacg gcctcttcat ggtcaactca gacacctact accgcaatta ccaggagctg
     5701 tggctgtccc gggagaatca gaagctgctc aagaagctct tcagcatcaa gtacgagaag
     5761 acacagatga accacgatgc cctccaggtg tacaaggcca tcattgacca ggtcgagaag
     5821 ttcttcaagc tgtacgatat caatcagttc agggcgaagc tctccgacgc cattgagcgc
     5881 ttcgagaagc tgccgatcaa caccgatggc aataagattg gcaagaccga gacactccgg
     5941 cagatcctca ttggcctcca ggcgaacggc acaaggtcca acgtgaagaa tctgggcatc
     6001 aagaccgacc tcggcctgct ccaggtcggc agcggcatca agctcgacaa ggatacccag
     6061 attgtctacc agtcaccgtc tggcctgttc aagaggcgca tcccactcgc cgacctgggc
     6121 agttccggcg ggtcctcaag ggctgatcct aagaagaaga ggaaggtttc tagggcagat
     6181 ccaaagaaga agcggaaggt gtctagagct gatccgaaga agaagcgcaa ggtgcctagg
     6241 ggaggatctg gtggtctttt ggatccagga acccctatgg atgctgacct tgttgcttct
     6301 tccaccgttg tctgggagca ggatgccgac cctttcgctg gtaccgcaga cgacttccca
     6361 gcttttaacg aggaagagct tgcttggctc atggaattgt tgccacaggg aggttctggt
     6421 ggattgttgg atcctggaac tccaatggac gctgatcttg ttgcttcctc tactgtcgtt
     6481 tgggagcaag acgctgaccc tttcgctgga accgctgacg atttcccagc ctttaacgaa
     6541 gaggaattgg cctggcttat ggagttgctt cctcaggcta ggggaggatc tggtggtctt
     6601 ttggatccag gaacccctat ggatgctgac cttgttgctt cttccaccgt tgtctgggag
     6661 caggatgccg accctttcgc tggtaccgca gacgacttcc cagcttttaa cgaggaagag
     6721 cttgcttggc tcatggaatt gttgccacag ggaggttctg gtggattgtt ggatcctgga
     6781 actccaatgg acgctgatct tgttgcttcc tctactgtcg tttgggagca agacgctgac
     6841 cctttcgctg gaaccgctga cgatttccca gcctttaacg aagaggaatt ggcctggctt
     6901 atggagttgc ttcctcaggc taggggagga tctggtggtc ttttggatcc aggaacccct
     6961 atggatgctg accttgttgc ttcttccacc gttgtctggg agcaggatgc cgaccctttc
     7021 gctggtaccg cagacgactt cccagctttt aacgaggaag agcttgcttg gctcatggaa
     7081 ttgttgccac agggaggttc tggtggattg ttggatcctg gaactccaat ggacgctgat
     7141 cttgttgctt cctctactgt cgtttgggag caagacgctg accctttcgc tggaaccgct
     7201 gacgatttcc cagcctttaa cgaagaggaa ttggcctggc ttatggagtt gcttcctcag
     7261 gctaggggtg gatctggagg tggtggtagc ggaggagatg ctcttgatga tttcgatctt
     7321 gatatgcttg gatctgacgc tctcgacgac tttgacctcg acatgttggg atctgatgct
     7381 cttgacgatt tcgatttgga catgttgggt tctgacgcct tggacgattt cgacctcgac
     7441 atgcttgcta ggggatctga cgcccttgat gatttcgact tggatatgct tggatctgac
     7501 gcccttgatg atttcgactt ggatatgctt ggatctgacg cccttgatga tttcgacttg
     7561 gatatgcttg gatctgacgc ccttgatgat ttcgacttgg atatgctttg actgcagata
     7621 tgaagatgaa gatgaaatat ttggtgtgtc aaataaaaag cttgtgtgct taagtttgtg
     7681 tttttttctt ggcttgttgt gttatgaatt tgtggctttt tctaatatta aatgaatgta
     7741 agatcacatt ataatgaata aacaaatgtt tctataatcc attgtgaatg ttttgttgga
     7801 tctcttctgc agcatataac tactgtatgt gctatggtat ggactatgga atatgattaa
     7861 agataagagg atccgcggat catgaaccaa cggcctggct gtatttggtg gttgtgtagg
     7921 gagatgggga gaagaaaagc ccgattctct tcgctgtgat gggctggatg catgcggggg
     7981 agcgggaggc ccaagtacgt gcacggtgag cggcccacag ggcgagtgtg agcgcgagag
     8041 gcgggaggaa cagtttagta ccacattgcc cagctaactc gaacgcgacc aacttataaa
     8101 cccgcgcgct gtcgcttgtg tggagacctt atattcccca gaacatcagg ttaatggcgt
     8161 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggaaaccg
     8221 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
     8281 ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca
     8341 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc
     8401 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca
     8461 aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
     8521 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg
     8581 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg
     8641 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata
     8701 tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttggtaccg
     8761 gtctcagtct caggtagatg tcagatcaat cagaaatgat tgatctgaca tctacgagtt
     8821 gagatcaaac aaagcttcag ctgagtttca atttctgagc ccatgttggg ccatacatat
     8881 gccacccgag tgcaaatcgg gtggcttttt ttctagaccc agctttcttg tacaaagttg
     8941 gcattactgc gagctcaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     9001 agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt
     9061 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     9121 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     9181 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     9241 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     9301 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     9361 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     9421 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     9481 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     9541 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     9601 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     9661 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     9721 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     9781 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     9841 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     9901 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     9961 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
    10021 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
    10081 agccgcccgc gcacggctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
    10141 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
    10201 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
    10261 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
    10321 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
    10381 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
    10441 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
    10501 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
    10561 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
    10621 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
    10681 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
    10741 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
    10801 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
    10861 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
    10921 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
    10981 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
    11041 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
    11101 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
    11161 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
    11221 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
    11281 gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc
    11341 cgaggaatcg gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
    11401 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
    11461 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
    11521 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
    11581 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
    11641 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
    11701 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
    11761 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
    11821 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
    11881 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
    11941 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
    12001 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
    12061 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
    12121 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
    12181 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
    12241 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
    12301 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
    12361 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
    12421 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
    12481 gcctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
    12541 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
    12601 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
    12661 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
    12721 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
    12781 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
    12841 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
    12901 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggaaacggt cacagcttgt
    12961 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    13021 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
    13081 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    13141 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
    13201 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    13261 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    13321 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    13381 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    13441 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    13501 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    13561 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    13621 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    13681 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    13741 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    13801 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    13861 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    13921 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    13981 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
    14041 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
    14101 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
    14161 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
    14221 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
    14281 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
    14341 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
    14401 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
    14461 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
    14521 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
    14581 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
    14641 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
    14701 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
    14761 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
    14821 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
    14881 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
    14941 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
    15001 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
    15061 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
    15121 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
    15181 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
    15241 ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac
    15301 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
    15361 ttagtactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
    15421 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc
    15481 ctaattccct tatctgggaa ctactcacac attattatgg agaaaataga gcttgtcgat
    15541 cgttaattaa tcacctccaa gatcccggac gagtgctggg gcgtcggttt ccactatcgg
    15601 cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac
    15661 gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg
    15721 catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca
    15781 tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg
    15841 tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt
    15901 gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg
    15961 tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct
    16021 cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca
    16081 tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg
    16141 tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat
    16201 cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg
    16261 tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc
    16321 tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt
    16381 gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat
    16441 atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca
    16501 tcaggtcgga cacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga
    16561 gttcaggctt tttcatccta ggagagatag atttgtagag agagactggt gatttcagcg
    16621 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga aggatagtgg
    16681 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
    16741 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
    16801 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
    16861 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
    16921 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
    16981 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgtta
    17041 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
    17101 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
    17161 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
    17221 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
    17281 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
    17341 acgagagtgt cgtgctccac catg
//
