LOCUS       Exported                4733 bp ds-DNA     circular SYN 29-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 12, 2023 from SnapGene 4.3.6
            https://www.snapgene.com
COMMENT     Insert from pCR8-PUFcNoNLS_NES-ADARdd(E488Q)-26-129-64 652 to 3113
            Backbone from pAC90-pmaxGW-D55-60,22-61-69,23-91-7 2650 to 967
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..947
                     /organism="pmaxCloning"
                     /mol_type="other DNA"
                     /label=source:pmaxCloning
     source          960..967
                     /organism="pLenti6/Ubc/V5-DEST Gateway (ViraPower)"
                     /mol_type="other DNA"
                     /label=source:pLenti6/Ubc/V5-DEST Gateway (ViraPower)
     source          968..991
                     /organism="pCR8/GW/TOPO"
                     /mol_type="other DNA"
                     /label=source:pCR8/GW/TOPO
                     /label=source:pCR8/GW/TOPO(1)
     source          1071..1466
                     /organism="MS2-P65-HSF1_GFP"
                     /mol_type="other DNA"
                     /label=source:MS2-P65-HSF1_GFP
     source          2762..2772
                     /organism="pCR8/GW/TOPO"
                     /mol_type="other DNA"
                     /label=source:pCR8/GW/TOPO
     source          2773..2893
                     /organism="pLenti6/Ubc/V5-DEST Gateway (ViraPower)"
                     /mol_type="other DNA"
                     /label=source:pLenti6/Ubc/V5-DEST Gateway (ViraPower)
                     /label=source:pLenti6/Ubc/V5-DEST Gateway (ViraPower)(1)
     source          2906..4733
                     /organism="pmaxCloning"
                     /mol_type="other DNA"
                     /label=source:pmaxCloning
                     /label=source:pmaxCloning(1)
     promoter        163..715
                     /label=CMV_immearly_promoter
     misc_feature    218..505
                     /label=CAG_enhancer
     promoter        661..742
                     /label=CMV_promoter
     misc_feature    672..692
                     /label=CMV_fwd_primer
     misc_feature    813..945
                     /label=chim_int
     misc_feature    960..967
                     /label=attR1
     misc_feature    complement(968..984)
                     /label=attL1
     misc_feature    987..991
                     /label=EcoRIHS
     CDS             1071..1466
                     /codon_start=1
                     /label=ORF frame 2
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKV
                     TCSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCEL
                     IVKAMQGLLKDGNPIPSAIAANSGIYSA"
     misc_feature    1530..1562
                     /label=NES
     misc_feature    1584..2738
                     /label=ADARdd(E488Q)
     misc_feature    2763..2772
                     /label=attL2
     misc_feature    2840..2881
                     /label=V5
     misc_feature    complement(2840..2859)
                     /label=AC_V5_R
     misc_feature    complement(2907..2926)
                     /label=EBV_rev_primer
     rep_origin      complement(3200..3818)
                     /direction=LEFT
                     /label=pBR322_origin
     CDS             complement(3883..4728)
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MGGDRDETNPDKCFNNIMIEQDGLHAGSPAAWVERLFGYDWAQQT
                     IGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEA
                     GRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERART
                     RMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSG
                     FIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF
                     "
     gene            complement(3886..4665)
                     /gene="NeoR/KanR (variant)"
                     /label=NeoR/KanR (variant)
                     /note="/translation=MGGDRDETNPDKCFNNIMIEQDGLHAGSPAAWVERLFGY
                     DWAQQ 
                     TIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVT 
                     EAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIER 
                     ARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENG 
                     RFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRL 
                     LDEFF*"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg
      721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt
      841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg
      901 cacctattgg tcttactgac atccactttg cctttctctc cacagggaaa aaacaattga
      961 caagtttgta caaaaaagca ggctccgaat tcaccggtgc cgccaccatg aacgttggtg
     1021 gtggtggaag cgggggtggg ggcagcggtg gagggggaag cgggcgcgcc atggcttcaa
     1081 actttactca gttcgtgctc gtggacaatg gtgggacagg ggatgtgaca gtggctcctt
     1141 ctaatttcgc taatggggtg gcagagtgga tcagctccaa ctcacggagc caggcctaca
     1201 aggtgacatg cagcgtcagg cagtctagtg cccagaagag aaagtatacc atcaaggtgg
     1261 aggtccccaa agtggctacc cagacagtgg gcggagtcga actgcctgtc gccgcttgga
     1321 ggtcctacct gaacatggag ctcactatcc caattttcgc taccaattct gactgtgaac
     1381 tcatcgtgaa ggcaatgcag gggctcctca aagacggtaa tcctatccct tccgccatcg
     1441 ccgctaactc aggtatctac agcgctggcg gccgcggggg aggcggttcc ggtggcggcg
     1501 gaagcggagg tggaggatca gggccggccc tgcagctgcc tccacttgaa agactgacac
     1561 tgggatccgg aggaggtgga agccagctgc atttaccgca ggttttagct gacgctgtct
     1621 cacgcctggt cctgggtaag tttggtgacc tgaccgacaa cttctcctcc cctcacgctc
     1681 gcagaaaagt gctggctgga gtcgtcatga caacaggcac agatgttaaa gatgccaagg
     1741 tgataagtgt ttctacagga acaaaatgta ttaatggtga atacatgagt gatcgtggcc
     1801 ttgcattaaa tgactgccat gcagaaataa tatctcggag atccttgctc agatttcttt
     1861 atacacaact tgagctttac ttaaataaca aagatgatca aaaaagatcc atctttcaga
     1921 aatcagagcg aggggggttt aggctgaagg agaatgtcca gtttcatctg tacatcagca
     1981 cctctccctg tggagatgcc agaatcttct caccacatga gccaatcctg gaagaaccag
     2041 cagatagaca cccaaatcgt aaagcaagag gacagctacg gaccaaaata gagtctggtc
     2101 aggggacgat tccagtgcgc tccaatgcga gcatccaaac gtgggacggg gtgctgcaag
     2161 gggagcggct gctcaccatg tcctgcagtg acaagattgc acgctggaac gtggtgggca
     2221 tccagggatc actgctcagc attttcgtgg agcccattta cttctcgagc atcatcctgg
     2281 gcagccttta ccacggggac cacctttcca gggccatgta ccagcggatc tccaacatag
     2341 aggacctgcc acctctctac accctcaaca agcctttgct cagtggcatc agcaatgcag
     2401 aagcacggca gccagggaag gcccccaact tcagtgtcaa ctggacggta ggcgactccg
     2461 ctattgaggt catcaacgcc acgactggga aggatgagct gggccgcgcg tcccgcctgt
     2521 gtaagcacgc gttgtactgt cgctggatgc gtgtgcacgg caaggttccc tcccacttac
     2581 tacgctccaa gattaccaag cccaacgtgt accatgagtc caagctggcg gcaaaggagt
     2641 accaggccgc caaggcgcgt ctgttcacag ccttcatcaa ggcggggctg ggggcctggg
     2701 tggagaagcc caccgagcag gaccagttct cactcacgaa cgtttgatta attaagaatt
     2761 cgacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct
     2821 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     2881 gcgtaccggt tagcaattgt tttttcgatg agtttggaca aaccacaact agaatgcagt
     2941 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3001 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3061 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtact taagaggggg
     3121 agaccaaagg gcgagacgtt aaggcctcac gtgacatgtg agcaaaaggc cagcaaaagg
     3181 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3241 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3301 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3361 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3421 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3481 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3541 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3601 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3661 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3721 gatccggcaa acaaaccacg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3841 gtggaacgaa aactcacgtt aagggatttt ggtcatgccg tctcagaaga actcgtcaag
     3901 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     3961 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     4021 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     4081 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
     4141 gggcatgctc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     4201 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     4261 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     4321 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     4381 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     4441 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag
     4501 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     4561 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     4621 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcataat
     4681 attattgaag catttatcag ggttcgtctc gtcccggtct cctcccatgc atg
//
