LOCUS       Exported                4642 bp ds-DNA     circular SYN 22-DEC-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 12, 2023 from SnapGene 4.3.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..675
                     /organism="pCR8/GW/TOPO"
                     /mol_type="other DNA"
                     /label=source:pCR8/GW/TOPO
     source          683..859
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
     source          join(932..939,2395..2400)
                     /label=Human U6 Promoter
                     /label=Human U6 Promoter(1)
                     /note="/dnas_title=PX335a-U6-chimeric_BB(+85)-CBh-NLS-hSpCs
                     n1( D10A)-NLS"
     source          join(940..966,968..979,1286..1293,1295..1511,1513..1701,
                     1703..1983,1985..2315,2317..2394)
                     /organism="pLenti6/Ubc/V5-DEST Gateway (ViraPower)"
                     /mol_type="other DNA"
                     /label=source:pLenti6/Ubc/V5-DEST Gateway (ViraPower)
     source          join(980..1066,1068..1285)
                     /organism="pLenti6/Ubc/V5-DEST Gateway (ViraPower)"
                     /mol_type="other DNA"
                     /label=source:pLenti6/Ubc/V5-DEST Gateway (ViraPower)
                     /label=source:pLenti6/Ubc/V5-DEST Gateway (ViraPower)(1)
     source          2401..2402
                     /label=Human U6 Promoter
                     /note="/dnas_title=PX335a-U6-chimeric_BB(+85)-CBh-NLS-hSpCs
                     n1( D10A)-NLS"
     source          2520..4642
                     /organism="pCR8/GW/TOPO"
                     /mol_type="other DNA"
                     /label=source:pCR8/GW/TOPO
                     /label=source:pCR8/GW/TOPO(1)
     terminator      complement(268..295)
                     /label=rrnB_T2_terminator
     terminator      complement(427..470)
                     /label=rrnB_T1_terminator
     misc_feature    522..544
                     /label=M13_pUC_fwd_primer
     misc_feature    537..553
                     /label=M13_forward20_primer
     misc_feature    complement(569..668)
                     /label=attL1
     misc_feature    671..675
                     /label=EcoRIHS
     promoter        683..859
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     683..703
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     854..859
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    934..939
                     /label=BbsI
     misc_feature    complement(join(980..1066,1068..1285))
                     /label=ccdB
     misc_feature    complement(1083..1102)
                     /label=AC_S105:hUbcAttRBGHPolyARSeq
     misc_feature    complement(1232..1251)
                     /label=AC_S98:ccdbFSeq
     misc_feature    join(1492..1511,1513)
                     /label=AC_S104:hUbcAttRBGHPolyARSeq
     gene            complement(join(1627..1701,1703..1983,1985..2286))
                     /gene="CAT/CamR"
                     /label=CAT/CamR
     CDS             complement(join(1627..1701,1703..1983,1985..2286))
                     /codon_start=1
                     /gene="CAT/CamR"
                     /label=CAT/CamR
                     /label=CAT/CamR(1)
                     /note="ORF frame 2"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFHR
                     SGVNTTTISGSFYTYIRKMWRVTVKTWPISLKGLLRICFSSQPIPG*VSPVLI*TWPIW
                     TTSSPPFSPWANIIRKATRC*CRWRFRFIMPL*WLPCRQNA**ITTVLR*VAGRGV"
     misc_feature    complement(1702)
                     /label=pLentiSrcReSeqC->TSymInCamR
     misc_feature    complement(1891..1910)
                     /label=AC_S97:CamRFSeq1
     misc_feature    complement(2316)
                     /label=pLentiSrcReseq.G->T
     promoter        complement(2347..2370)
                     /label=lac_promoter
     misc_feature    2395..2400
                     /label=BbsI
                     /label=BbsI(1)
     misc_feature    2403..2438
                     /label=PspCas13b_DR
     misc_feature    complement(2481..2490)
                     /label=stabilizer_AS
     misc_feature    2521..2620
                     /label=attL2
     promoter        complement(2638..2656)
                     /label=T7_promoter
     misc_feature    complement(2657..2675)
                     /label=M13_reverse_primer
     CDS             2889..3899
                     /codon_start=1
                     /gene="spect"
                     /label=spect
                     /note="ORF frame 2"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            2889..3899
                     /gene="spect"
                     /label=spect
                     /label=spect(1)
     rep_origin      3976..4595
                     /label=pBR322_origin
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgatca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtattcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcaccg gtgagggcct atttcccatg attccttcat atttgcatat
      721 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
      781 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
      841 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
      901 tttatatatc ttgtggaaag gacgaaacac cgggtcttcg tcgacctgca gactggctgt
      961 gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt
     1021 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggacacc ggcacactgg
     1081 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
     1141 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
     1201 cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
     1261 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg
     1321 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
     1381 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
     1441 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
     1501 gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat
     1561 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
     1621 agatctttac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1681 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1741 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1801 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1861 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1921 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1981 aaaggtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     2041 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
     2101 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     2161 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     2221 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     2281 ctccatttta gcttccttag ctcctgaaaa tctcgacgga tcctaactca aaatccacac
     2341 attatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgcgg ccgcgaagac
     2401 ctgttgtgga aggtccagtt ttgaggggct attacaacca attgggtctc cagatgcgta
     2461 caccatcagg gtacgcagat ctcgtattcg tttttttggc gcgccttaat taagaattcg
     2521 acccagcttt cttgtacaaa gttggcatta taaaaaataa ttgctcatca atttgttgca
     2581 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     2641 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     2701 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     2761 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga
     2821 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     2881 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     2941 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     3001 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     3061 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
     3121 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     3181 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     3241 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     3301 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     3361 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     3421 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     3481 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     3541 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     3601 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     3661 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     3721 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     3781 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     3841 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     3901 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     3961 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4021 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4081 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     4141 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4261 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4321 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4381 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4441 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4501 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4561 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4621 gctggccttt tgctcacatg tt
//
