LOCUS       pβactin-mNeonGreen-CI        6773 bp    DNA     circular
            
ACCESSION   DNA_pCAG-mcherry-TUBA1B
VERSION     DNA_pCAG-mcherry-TUBA1B.0
KEYWORDS    .

COMMENT
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                    </fill>
                </style>
                <location x="113" y="278"/>
                <dimension height="312" width="312"/>
            </element>
            <element id="2030908845" name="SV40 early polyA signal"
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                type="25" xsi:type="label">
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            <element name="PolyA Signal" type="25" xsi:type="annotation">
                <label text="">
                    <style name="Signal Label" xsi:type="basicStyle">
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                            <font name="Arial" size="9" style="1"/>
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                            <color blue="255" green="255" red="255"/>
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                </symbol>
            </element>
            <element id="1712103067" name="AmpR"
                target="AmpR:4338 - 3478" text="@N" type="4" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
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                <location x="158" y="593"/>
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            <element id="1712103067" name="AmpR"
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                <style name="Shape 3" shape="1" xsi:type="basicStyle">
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                <location x="88" y="253"/>
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            <element id="851831011" name="mNeonGreen"
                target="mNeonGreen:1269 - 1976" text="@N" type="4" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
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                <location x="449" y="411"/>
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            <element id="851831011" name="mNeonGreen"
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            <element id="851831011" name="mNeonGreen" shape="1"
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                <style name="Shape 3" shape="1" xsi:type="basicStyle">
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                        <pattern style="1"/>
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                    <fill pattern="Solid">
                        <color blue="102" green="153" red="255"/>
                    </fill>
                </style>
                <location x="88" y="253"/>
                <dimension height="362" width="362"/>
            </element>
            <element id="1330780622" name="intron of chicken Beta-actin"
                target="intron of chicken Beta-actin:430 - 1239"
                text="@N" type="15" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
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            <element id="1330780622" name="intron of chicken Beta-actin"
                target="intron of chicken Beta-actin:430 - 1239"
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            <element id="1330780622" name="intron of chicken Beta-actin"
                shape="0"
                target="intron of chicken Beta-actin:430 - 1239"
                type="15" xsi:type="symbol">
                <style name="Line 1" shape="0" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="255" green="0" red="0"/>
                        <pattern style="1"/>
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                    <fill pattern="Solid">
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                </style>
                <location x="88" y="253"/>
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            </element>
            <element name="Intron" type="15" xsi:type="annotation">
                <label text="">
                    <style name="Signal Label" xsi:type="basicStyle">
                        <label text="@N">
                            <font name="Arial" size="9" style="1"/>
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                </label>
                <connector>
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                        <label text="@N">
                            <font name="Arial" size="9" style="1"/>
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                </connector>
                <symbol shape="0">
                    <style name="Line 1" xsi:type="basicStyle">
                        <line width="1">
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                        <fill pattern="Solid">
                            <color blue="255" green="255" red="255"/>
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                    </style>
                </symbol>
            </element>
            <element id="1178712158" name="chicken Beta-actin"
                target="chicken Beta-actin:4879 - 238" text="@N"
                type="29" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
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                </style>
                <location x="64" y="293"/>
            </element>
            <element id="1178712158" name="chicken Beta-actin"
                target="chicken Beta-actin:4879 - 238" type="29" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
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                        <color blue="0" green="0" red="0"/>
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            </element>
            <element id="1178712158" name="chicken Beta-actin" shape="1"
                target="chicken Beta-actin:4879 - 238" type="29" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
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                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="88" y="253"/>
                <dimension height="362" width="362"/>
            </element>
            <element id="1576221393" target="1576221393" text="@L bp"
                type="-2055714368" xsi:type="label">
                <style name="Molecule Label 2" xsi:type="basicStyle">
                    <label text="@L bp">
                        <font name="Arial" size="18" style="0"/>
                        <color blue="50" green="50" red="50"/>
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                </style>
                <location x="239" y="438"/>
            </element>
            <element id="1167677204" target="2055105" type="2055105" xsi:type="symbol">
                <style name="Axis" xsi:type="basicStyle">
                    <line width="2">
                        <color blue="153" green="204" red="204"/>
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                    <fill pattern="Solid">
                        <color blue="204" green="255" red="255"/>
                    </fill>
                </style>
                <location x="119" y="284"/>
                <dimension height="300" width="300"/>
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            <element id="1954203131" target="1954203131" text="@N"
                type="346519525" xsi:type="label">
                <style name="Molecule Label 1" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="24" style="1"/>
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                    </label>
                </style>
                <location x="131" y="410"/>
            </element>
        </elements>
    </setupInfo>
    <setupInfo complete="true" completeMinSize="150"
        completeNested="false" includeStopCodon="false"
        incomplete="false" incompleteMinSize="30" selected="false"
        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
        undefinedStart="false" undefinedStop="false" undefinedStopNested="false"/>
    <setupInfo allowBlunt="true" allowFivePrime="true"
        allowThreePrime="true"
        enzymeMap="BamHI|ggatcc,AvaI|cycgrg,KpnI|ggtacc,XhoI|ctcgag,AflII|cttaag,SmaI|cccggg,ApaLI|gtgcac,ApaI|gggccc,NcoI|ccatgg,SalI|gtcgac,ClaI|atcgat,EcoRI|gaattc,NotI|gcggccgc,SacI|gagctc,XmaI|cccggg,AgeI|accggt,BglII|agatct,HindIII|aagctt,BspEI|tccgga,NheI|gctagc,XbaI|tctaga,NdeI|catatg,PstI|ctgcag"
        enzymesCleavePoint="BamHI|2,AvaI|2,KpnI|6,XhoI|2,AflII|2,SmaI|4,ApaLI|2,ApaI|6,NcoI|2,SalI|2,ClaI|3,EcoRI|2,NotI|3,SacI|6,XmaI|2,AgeI|2,BglII|2,HindIII|2,BspEI|2,NheI|2,XbaI|2,NdeI|3,PstI|6"
        enzymesComplCleavePoint="BamHI|0,AvaI|0,KpnI|0,XhoI|0,AflII|0,SmaI|0,ApaLI|0,ApaI|0,NcoI|0,SalI|0,ClaI|0,EcoRI|0,NotI|0,SacI|0,XmaI|0,AgeI|0,BglII|0,HindIII|0,BspEI|0,NheI|0,XbaI|0,NdeI|0,PstI|0"
        enzymesPalindromic="BamHI|true,AvaI|true,KpnI|true,XhoI|true,AflII|true,SmaI|true,ApaLI|true,ApaI|true,NcoI|true,SalI|true,ClaI|true,EcoRI|true,NotI|true,SacI|true,XmaI|true,AgeI|true,BglII|true,HindIII|true,BspEI|true,NheI|true,XbaI|true,NdeI|true,PstI|true"
        ignoreIn="-1,-1" ignoreLess="-1" ignoreMore="-1"
        ignoreOut="-1,-1" selected="true" startIndex="1" type="Restriction Map"/>
    <setupInfo featureList="" selected="true" type="Feature Map"/>
    <setupInfo basesPerBlock="10" blocksPerLine="10"
        prefer1LetterAACode="false" preferSingleStrand="false"
        selected="true" textBold="false" textColor="0,0,0"
        textFontName="Andale Mono" textFontSize="14" textItalic="false"
        textUnderline="false" trackSeparatorColor="128,128,128"
        trackSeparatorWidth="2" type="Sequence"
        xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:type="sequenceSetup">
        <textRangeSetupList>
            <textRangeSetup backgroundColor="255,0,128" bold="false"
                fontName="" fontSize="0" italic="false"
                startIndex="1268" stopIndex="1268" underline="false"/>
            <textRangeSetup backgroundColor="230,230,230" bold="false"
                fontName="" fontSize="0" italic="false"
                startIndex="1997" stopIndex="3349" underline="false"/>
            <textRangeSetup backgroundColor="0,249,0" bold="false"
                fontName="" fontSize="0" italic="false"
                startIndex="1976" stopIndex="1976" underline="false"/>
            <textRangeSetup backgroundColor="0,249,0" bold="false"
                fontName="" fontSize="0" italic="false"
                startIndex="1269" stopIndex="1975" underline="false"/>
        </textRangeSetupList>
    </setupInfo>
    <setupInfo analysisSubsets="Analysis Results (MAIN)" selected="true" type="Auto Load Analysis"/>
</vntiDisplaySetup>
DISPLAY_SETUP_END|
COMMENT     
            VNTSEQSTYLE|1268|1268||0|null|null|null|255,0,128|null
            VNTSEQSTYLE|1997|3349||0|null|null|null|230,230,230|null
            VNTSEQSTYLE|1976|1976||0|null|null|null|0,249,0|null
            VNTSEQSTYLE|1269|1975||0|null|null|null|0,249,0|null
FEATURES             Location/Qualifiers
     CDS             complement(3478..4338)
                     /label=AmpR
                     /type="CDS"
     polyA_signal    2062..2290
                     /label=SV40 early polyA signal
                     /type="polyA_signal"
     eukaryotic      4879..238
                     /label=chicken Beta-actin
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
     intron          430..1239
                     /label=intron of chicken Beta-actin
                     /type="intron"
     CDS             1269..1976
                     /label=mNeonGreen
                     /type="CDS"
                     /vntifkey="4"
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcgctaccgg
     1261 tcgccaccat ggtgagcaag ggcgaggagg ataacatggc ctctctccca gcgacacatg
     1321 agttacacat ctttggctcc atcaacggtg tggactttga catggtgggt cagggcaccg
     1381 gcaatccaaa tgatggttat gaggagttaa acctgaagtc caccaagggt gacctccagt
     1441 tctccccctg gattctggtc cctcatatcg ggtatggctt ccatcagtac ctgccctacc
     1501 ctgacgggat gtcgcctttc caggccgcca tggtagatgg ctccggctac caagtccatc
     1561 gcacaatgca gtttgaagat ggtgcctccc ttactgttaa ctaccgctac acctacgagg
     1621 gaagccacat caaaggagag gcccaggtga aggggactgg tttccctgct gacggtcctg
     1681 tgatgaccaa ctcgctgacc gctgcggact ggtgcaggtc gaagaagact taccccaacg
     1741 acaaaaccat catcagtacc tttaagtgga gttacaccac tggaaatggc aagcgctacc
     1801 ggagcactgc gcggaccacc tacacctttg ccaagccaat ggcggctaac tatctgaaga
     1861 accagccgat gtacgtgttc cgtaagacgg agctcaagca ctccaagacc gagctcaact
     1921 tcaaggagtg gcaaaaggcc tttaccgatg tgatgggcat ggacgagctg tacaagtccg
     1981 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
     2041 ccaccggatc tagataagta atgatcataa tcagccatat cacatctgta gaggttttac
     2101 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     2161 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     2221 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     2281 atgtatctta tcatgtctgg atcaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     2341 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     2401 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     2461 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     2521 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2581 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2641 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2701 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2761 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2821 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2881 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2941 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3001 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3061 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3121 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3181 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3241 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3301 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3361 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3421 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3481 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3541 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3601 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3661 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3721 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3781 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3841 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3901 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3961 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4021 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4081 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4141 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4201 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4261 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4321 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4381 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4441 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     4501 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     4561 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     4621 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     4681 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     4741 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     4801 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     4861 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     4921 aacgacggcc agtgccaagt tgggatcttt gcattggccc acggctctca ggatggggat
     4981 gctccccttc agcacccggt tccccttgga aactgatggt cctggctctg tggcatggca
     5041 gtggcactgt gaggagcccc ctaccagcag cacacagtgg ggttggcact gccacgctcc
     5101 ggatgccgcg ctctgatcca accccataat caagggaacc cgaattgccc catcattgcc
     5161 cccaccaccc ccatcctgcc gggccctcac accccacgct gccttctggt gacattcccc
     5221 agcccaaacc cacggcttca tggctaccgc ggggcatttc ccattgccgc cccattatca
     5281 gctctgcaca cctcccgctg tacccatgcc tcgtggctgc ccttctttga cgtataatct
     5341 tctaattaat acccggcctt gtcaaagtgg agcacaaacg ttaattaatt ccccagcagg
     5401 caggtaatta acagtgtgac tccctttttg ctgcgagtgg ggctgataca gagagatgtg
     5461 gcactatgga gcccacgggg tcctggcact gggtgcccac ggaggtcccc atgtgctgca
     5521 gtgtcaccgc ctccgaggag acagtattgt ccctgcggtg tccctgcagc tcagctctgt
     5581 ccacagggcc acctccagtt tggaggggac acaatgcagc cccgatgcaa cccatcctcg
     5641 cagcatccca gggacaaaga ccccactgca agaccgcaca cagggctggg tcccgctccc
     5701 ctaatatcta cagtgcttct tgcatggccc cttaatcaat gcagttaatc agcatgcgct
     5761 catgcaccgc tctggagctg caaagcccct cgcagcgctg ctcaccaaca ccgcgcaccg
     5821 ccccggccca gcctgcagca cgcgctgcaa acaggaaaga aacaaaatat tgcccaaatg
     5881 taggcaaagg cattcggctg ccttgacctc cgccgggccg ggccctgcct gactcagctc
     5941 cttactcagc gctcgcttcc tccctccggc tgccaccgcc gcagcgcaca ccctgacaaa
     6001 gagtggccct taacgggctc tgaggtgcac ccagcagtgc actcagcagt ccaagggccg
     6061 gcctggaggt ttgcaccgct acgtgctgac attagcattg aacttggccc tgggtagtgc
     6121 tgcaggccgg gcggggtggg tgtagagagt gcagcgcgcg ttgcacccgg tgccccttcc
     6181 cctcccttgc atcccagcag gctgcacccc agcaccaggc ccgtgcatgc atgctcctgg
     6241 tgttattgca gcctggtgca tgcatgcgtc ttgatggtgc agcgctgtgc atgcatcctc
     6301 cttggtgtgt agcagtctag tgcatgcata cccctcggtg ttattgctgc tctgtgcacg
     6361 cacgctcatt gtatcacttc atcccagtgc atgcactcac actggagcga ttgctgctcg
     6421 gtgcacgcac actcattgta tcactgcagc tcagtggctg cacgcacacc ggtgttattg
     6481 ctgctcggtg cgtgcatgca catcagtgtc gctgcagctc agtgcatgca cgctcattgc
     6541 ccatcgctat ccctgcctct cctgctggcg ctccccggga ggtgacttca aggggaccgc
     6601 aggaccacct cgggggtggg gggagggctg cacacgcgga ccccgctccc cctccccaac
     6661 aaagcactgt ggaatcaaaa aggggggagg ggggatggag gggcgcgtca cacccccgcc
     6721 ccacaccctc acctcgaggt gagccccacg ttctgcttca ctctccccat ctc
//
