LOCUS       pβactin-td-mCherry-CI        7471 bp    DNA     circular
            
ACCESSION   DNA_pCAG-mcherry-TUBA1B
VERSION     DNA_pCAG-mcherry-TUBA1B.0
KEYWORDS    .

COMMENT
DISPLAY_SETUP_START|
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            <element id="623950797" name="NdeI (2041)" shape="14"
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                <location x="122" y="330"/>
                <dimension height="312" width="312"/>
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            <element id="319684869" name="AmpR"
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                <location x="132" y="620"/>
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            <element id="1787111579" name="intron of chicken Beta-actin"
                target="intron of chicken Beta-actin:430 - 1239"
                text="@N" type="15" xsi:type="label">
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            <element name="Intron" type="15" xsi:type="annotation">
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            </element>
            <element id="597155355" name="chicken Beta-actin"
                target="chicken Beta-actin:5577 - 238" text="@N"
                type="29" xsi:type="label">
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                        <font name="Arial" size="9" style="1"/>
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                <location x="82" y="337"/>
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            <element id="597155355" name="chicken Beta-actin"
                target="chicken Beta-actin:5577 - 238" type="29" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
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                        <color blue="0" green="0" red="0"/>
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            <element id="597155355" name="chicken Beta-actin" shape="1"
                target="chicken Beta-actin:5577 - 238" type="29" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
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                    <fill pattern="Solid">
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                </style>
                <location x="97" y="305"/>
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            </element>
            <element id="2105732793" target="2105732793" text="@L bp"
                type="-2055714368" xsi:type="label">
                <style name="Molecule Label 2" xsi:type="basicStyle">
                    <label text="@L bp">
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                <location x="248" y="490"/>
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            <element id="2025173753" target="2025173753" text="@N"
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                <location x="151" y="462"/>
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    </setupInfo>
    <setupInfo complete="true" completeMinSize="150"
        completeNested="false" includeStopCodon="false"
        incomplete="false" incompleteMinSize="30" selected="false"
        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
        undefinedStart="false" undefinedStop="false" undefinedStopNested="false"/>
    <setupInfo allowBlunt="true" allowFivePrime="true"
        allowThreePrime="true"
        enzymeMap="BamHI|ggatcc,AvaI|cycgrg,KpnI|ggtacc,XhoI|ctcgag,AflII|cttaag,SmaI|cccggg,ApaLI|gtgcac,ApaI|gggccc,NcoI|ccatgg,SalI|gtcgac,ClaI|atcgat,EcoRI|gaattc,NotI|gcggccgc,SacI|gagctc,XmaI|cccggg,AgeI|accggt,BglII|agatct,HindIII|aagctt,BspEI|tccgga,NheI|gctagc,XbaI|tctaga,NdeI|catatg,PstI|ctgcag"
        enzymesCleavePoint="BamHI|2,AvaI|2,KpnI|6,XhoI|2,AflII|2,SmaI|4,ApaLI|2,ApaI|6,NcoI|2,SalI|2,ClaI|3,EcoRI|2,NotI|3,SacI|6,XmaI|2,AgeI|2,BglII|2,HindIII|2,BspEI|2,NheI|2,XbaI|2,NdeI|3,PstI|6"
        enzymesComplCleavePoint="BamHI|0,AvaI|0,KpnI|0,XhoI|0,AflII|0,SmaI|0,ApaLI|0,ApaI|0,NcoI|0,SalI|0,ClaI|0,EcoRI|0,NotI|0,SacI|0,XmaI|0,AgeI|0,BglII|0,HindIII|0,BspEI|0,NheI|0,XbaI|0,NdeI|0,PstI|0"
        enzymesPalindromic="BamHI|true,AvaI|true,KpnI|true,XhoI|true,AflII|true,SmaI|true,ApaLI|true,ApaI|true,NcoI|true,SalI|true,ClaI|true,EcoRI|true,NotI|true,SacI|true,XmaI|true,AgeI|true,BglII|true,HindIII|true,BspEI|true,NheI|true,XbaI|true,NdeI|true,PstI|true"
        ignoreIn="-1,-1" ignoreLess="-1" ignoreMore="-1"
        ignoreOut="-1,-1" selected="true" startIndex="1" type="Restriction Map"/>
    <setupInfo featureList="" selected="true" type="Feature Map"/>
    <setupInfo basesPerBlock="10" blocksPerLine="10"
        prefer1LetterAACode="false" preferSingleStrand="false"
        selected="true" textBold="false" textColor="0,0,0"
        textFontName="Andale Mono" textFontSize="14" textItalic="false"
        textUnderline="false" trackSeparatorColor="128,128,128"
        trackSeparatorWidth="2" type="Sequence"
        xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:type="sequenceSetup">
        <textRangeSetupList>
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                fontName="" fontSize="0" italic="false"
                startIndex="2684" stopIndex="3349" underline="false"/>
            <textRangeSetup backgroundColor="255,38,0" bold="false"
                fontName="" fontSize="0" italic="false"
                startIndex="1269" stopIndex="1985" underline="false"/>
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                startIndex="2001" stopIndex="2683" underline="false"/>
            <textRangeSetup backgroundColor="255,38,0" bold="false"
                fontName="" fontSize="0" italic="false"
                startIndex="1986" stopIndex="2000" underline="false"/>
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    <setupInfo analysisSubsets="Analysis Results (MAIN)" selected="true" type="Auto Load Analysis"/>
</vntiDisplaySetup>
DISPLAY_SETUP_END|
COMMENT     
            VNTSEQSTYLE|2684|3349||0|null|null|null|230,230,230|null
            VNTSEQSTYLE|1269|1985||0|null|null|null|255,38,0|null
            VNTSEQSTYLE|1253|1268||0|null|null|null|255,38,0|null
            VNTSEQSTYLE|2001|2683||0|null|null|null|255,38,0|null
            VNTSEQSTYLE|1986|2000||0|null|null|null|255,38,0|null
FEATURES             Location/Qualifiers
     CDS             complement(4176..5036)
                     /label=AmpR
                     /type="CDS"
     polyA_signal    2760..2988
                     /label=SV40 early polyA signal
                     /type="polyA_signal"
     eukaryotic      5577..238
                     /label=chicken Beta-actin
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
     intron          430..1239
                     /label=intron of chicken Beta-actin
                     /type="intron"
     CDS             1253..2683
                     /label=td-mCherry
                     /type="CDS"
                     /vntifkey="4"
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcatggtcag
     1261 caaaggggaa gaggataaca tggccatcat caaagagttc atgcggttca aggtgcacat
     1321 ggaaggcagc gtgaacggac atgagttcga gatcgaagga gaaggagagg gacgccctta
     1381 cgagggaact cagaccgcaa agctgaaagt gactaagggt ggccctctgc cgtttgcgtg
     1441 ggacattctg tccccgcaat tcatgtatgg ctcaaaagcc tacgtcaaac acccagctga
     1501 catcccagac tacctgaaac tcagctttcc ggaaggattc aagtgggaac gcgtcatgaa
     1561 tttcgaagat ggcggagtgg tcaccgtgac tcaagacagc tcgttgcaag acggggagtt
     1621 tatctacaag gtgaagctcc ggggaactaa cttcccatca gacggtccgg tcatgcagaa
     1681 aaagacgatg ggttgggaag cctcctcgga gagaatgtac ccagaggacg gcgcgctgaa
     1741 gggtgaaatc aaacagagac tcaaactgaa agatggagga cactacgacg cggaagtcaa
     1801 gaccacctac aaggcaaaga aaccagtgca gttgccagga gcgtacaacg tgaacatcaa
     1861 acttgatatc acctcgcaca acgaagatta caccattgtg gagcaatacg aacgggcgga
     1921 aggaaggcat tcgaccgggg gaatggacga attatacaag ggctccggga gcggaatggt
     1981 gagcaaggga gaagaagata acatggctat tatcaaggaa tttatgcgct tcaaggttca
     2041 tatggagggc tcagtgaacg ggcacgaatt tgaaatcgag ggagagggag aaggcagacc
     2101 gtacgaaggc acccagactg ctaagctgaa ggttactaag ggaggacctc tgcccttcgc
     2161 atgggacatc ctttcgccgc agttcatgta cggttccaag gcttatgtga agcacccggc
     2221 agacattccg gattacctga agctgtcatt ccctgagggc tttaagtggg agcgcgtgat
     2281 gaactttgaa gatggtggag tggtgactgt gacccaagac tcctccctgc aagatgggga
     2341 gttcatctac aaagtcaagt tgcgcggaac taatttccct tcggacgggc cggtgatgca
     2401 gaagaaaact atgggatggg aagcttcatc ggaaaggatg taccccgagg acggagccct
     2461 gaaaggggag atcaagcagc ggctcaagct caaggatggc ggtcattacg acgccgaagt
     2521 caagaccacg tacaaggcca agaagccggt gcagctccct ggcgcgtaca atgtcaatat
     2581 caagctcgac atcacgtccc ataatgagga ttatactatc gtggaacagt acgagcgcgc
     2641 cgaaggacgg cactcaaccg gaggaatgga cgagctgtac aagagatctc gagctcaagc
     2701 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataagtaat
     2761 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
     2821 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     2881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     2941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     3001 caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3061 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3841 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5161 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     5401 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     5461 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     5521 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     5581 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagttg
     5641 ggatctttgc attggcccac ggctctcagg atggggatgc tccccttcag cacccggttc
     5701 cccttggaaa ctgatggtcc tggctctgtg gcatggcagt ggcactgtga ggagccccct
     5761 accagcagca cacagtgggg ttggcactgc cacgctccgg atgccgcgct ctgatccaac
     5821 cccataatca agggaacccg aattgcccca tcattgcccc caccaccccc atcctgccgg
     5881 gccctcacac cccacgctgc cttctggtga cattccccag cccaaaccca cggcttcatg
     5941 gctaccgcgg ggcatttccc attgccgccc cattatcagc tctgcacacc tcccgctgta
     6001 cccatgcctc gtggctgccc ttctttgacg tataatcttc taattaatac ccggccttgt
     6061 caaagtggag cacaaacgtt aattaattcc ccagcaggca ggtaattaac agtgtgactc
     6121 cctttttgct gcgagtgggg ctgatacaga gagatgtggc actatggagc ccacggggtc
     6181 ctggcactgg gtgcccacgg aggtccccat gtgctgcagt gtcaccgcct ccgaggagac
     6241 agtattgtcc ctgcggtgtc cctgcagctc agctctgtcc acagggccac ctccagtttg
     6301 gaggggacac aatgcagccc cgatgcaacc catcctcgca gcatcccagg gacaaagacc
     6361 ccactgcaag accgcacaca gggctgggtc ccgctcccct aatatctaca gtgcttcttg
     6421 catggcccct taatcaatgc agttaatcag catgcgctca tgcaccgctc tggagctgca
     6481 aagcccctcg cagcgctgct caccaacacc gcgcaccgcc ccggcccagc ctgcagcacg
     6541 cgctgcaaac aggaaagaaa caaaatattg cccaaatgta ggcaaaggca ttcggctgcc
     6601 ttgacctccg ccgggccggg ccctgcctga ctcagctcct tactcagcgc tcgcttcctc
     6661 cctccggctg ccaccgccgc agcgcacacc ctgacaaaga gtggccctta acgggctctg
     6721 aggtgcaccc agcagtgcac tcagcagtcc aagggccggc ctggaggttt gcaccgctac
     6781 gtgctgacat tagcattgaa cttggccctg ggtagtgctg caggccgggc ggggtgggtg
     6841 tagagagtgc agcgcgcgtt gcacccggtg ccccttcccc tcccttgcat cccagcaggc
     6901 tgcaccccag caccaggccc gtgcatgcat gctcctggtg ttattgcagc ctggtgcatg
     6961 catgcgtctt gatggtgcag cgctgtgcat gcatcctcct tggtgtgtag cagtctagtg
     7021 catgcatacc cctcggtgtt attgctgctc tgtgcacgca cgctcattgt atcacttcat
     7081 cccagtgcat gcactcacac tggagcgatt gctgctcggt gcacgcacac tcattgtatc
     7141 actgcagctc agtggctgca cgcacaccgg tgttattgct gctcggtgcg tgcatgcaca
     7201 tcagtgtcgc tgcagctcag tgcatgcacg ctcattgccc atcgctatcc ctgcctctcc
     7261 tgctggcgct ccccgggagg tgacttcaag gggaccgcag gaccacctcg ggggtggggg
     7321 gagggctgca cacgcggacc ccgctccccc tccccaacaa agcactgtgg aatcaaaaag
     7381 gggggagggg ggatggaggg gcgcgtcaca cccccgcccc acaccctcac ctcgaggtga
     7441 gccccacgtt ctgcttcact ctccccatct c
//
