LOCUS       pβactin-mOrange2-C1        6764 bp    DNA     circular
            
ACCESSION   DNA_pCAG-mcherry-TUBA1B
VERSION     DNA_pCAG-mcherry-TUBA1B.0
KEYWORDS    .

COMMENT
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            <element id="885789087" name="AmpR"
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                <location x="160" y="594"/>
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            <element id="1198573968" name="intron of chicken Beta-actin"
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            <element name="Intron" type="15" xsi:type="annotation">
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                </label>
                <connector>
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                <symbol shape="0">
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                            <color blue="255" green="255" red="255"/>
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                </symbol>
            </element>
            <element id="384780221" name="chicken Beta-actin"
                target="chicken Beta-actin:4870 - 238" text="@N"
                type="29" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
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                <location x="64" y="293"/>
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            <element id="384780221" name="chicken Beta-actin"
                target="chicken Beta-actin:4870 - 238" type="29" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
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                        <color blue="0" green="0" red="0"/>
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            <element id="384780221" name="chicken Beta-actin" shape="1"
                target="chicken Beta-actin:4870 - 238" type="29" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
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                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="88" y="253"/>
                <dimension height="362" width="362"/>
            </element>
            <element id="1595403770" target="1595403770" text="@L bp"
                type="-2055714368" xsi:type="label">
                <style name="Molecule Label 2" xsi:type="basicStyle">
                    <label text="@L bp">
                        <font name="Arial" size="18" style="0"/>
                        <color blue="50" green="50" red="50"/>
                    </label>
                </style>
                <location x="239" y="438"/>
            </element>
            <element id="798568582" target="2055105" type="2055105" xsi:type="symbol">
                <style name="Axis" xsi:type="basicStyle">
                    <line width="2">
                        <color blue="153" green="204" red="204"/>
                        <pattern style="1"/>
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                    <fill pattern="Solid">
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                    </fill>
                </style>
                <location x="119" y="284"/>
                <dimension height="300" width="300"/>
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            <element id="616893880" target="616893880" text="@N"
                type="346519525" xsi:type="label">
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                    <label text="@N">
                        <font name="Arial" size="24" style="1"/>
                        <color blue="50" green="50" red="50"/>
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                <location x="144" y="410"/>
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            <element id="1234414398" name="mOrange2"
                target="mOrange2:1260 - 1967" text="@N" type="21" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
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                <location x="449" y="410"/>
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            <element id="1234414398" name="mOrange2" shape="1"
                target="mOrange2:1260 - 1967" type="21" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
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                    <fill pattern="Solid">
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        </elements>
    </setupInfo>
    <setupInfo complete="true" completeMinSize="150"
        completeNested="false" includeStopCodon="false"
        incomplete="false" incompleteMinSize="30" selected="false"
        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
        undefinedStart="false" undefinedStop="false" undefinedStopNested="false"/>
    <setupInfo allowBlunt="true" allowFivePrime="true"
        allowThreePrime="true"
        enzymeMap="BamHI|ggatcc,AvaI|cycgrg,KpnI|ggtacc,XhoI|ctcgag,AflII|cttaag,SmaI|cccggg,ApaLI|gtgcac,ApaI|gggccc,NcoI|ccatgg,SalI|gtcgac,ClaI|atcgat,EcoRI|gaattc,NotI|gcggccgc,SacI|gagctc,XmaI|cccggg,AgeI|accggt,BglII|agatct,HindIII|aagctt,BspEI|tccgga,NheI|gctagc,XbaI|tctaga,NdeI|catatg,PstI|ctgcag"
        enzymesCleavePoint="BamHI|2,AvaI|2,KpnI|6,XhoI|2,AflII|2,SmaI|4,ApaLI|2,ApaI|6,NcoI|2,SalI|2,ClaI|3,EcoRI|2,NotI|3,SacI|6,XmaI|2,AgeI|2,BglII|2,HindIII|2,BspEI|2,NheI|2,XbaI|2,NdeI|3,PstI|6"
        enzymesComplCleavePoint="BamHI|0,AvaI|0,KpnI|0,XhoI|0,AflII|0,SmaI|0,ApaLI|0,ApaI|0,NcoI|0,SalI|0,ClaI|0,EcoRI|0,NotI|0,SacI|0,XmaI|0,AgeI|0,BglII|0,HindIII|0,BspEI|0,NheI|0,XbaI|0,NdeI|0,PstI|0"
        enzymesPalindromic="BamHI|true,AvaI|true,KpnI|true,XhoI|true,AflII|true,SmaI|true,ApaLI|true,ApaI|true,NcoI|true,SalI|true,ClaI|true,EcoRI|true,NotI|true,SacI|true,XmaI|true,AgeI|true,BglII|true,HindIII|true,BspEI|true,NheI|true,XbaI|true,NdeI|true,PstI|true"
        ignoreIn="-1,-1" ignoreLess="-1" ignoreMore="-1"
        ignoreOut="-1,-1" selected="true" startIndex="1" type="Restriction Map"/>
    <setupInfo featureList="" selected="true" type="Feature Map"/>
    <setupInfo basesPerBlock="10" blocksPerLine="10"
        prefer1LetterAACode="false" preferSingleStrand="false"
        selected="true" textBold="false" textColor="0,0,0"
        textFontName="Andale Mono" textFontSize="14" textItalic="false"
        textUnderline="false" trackSeparatorColor="128,128,128"
        trackSeparatorWidth="2" type="Sequence"
        xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:type="sequenceSetup">
        <textRangeSetupList>
            <textRangeSetup backgroundColor="230,230,230" bold="false"
                fontName="" fontSize="0" italic="false"
                startIndex="1979" stopIndex="3331" underline="false"/>
            <textRangeSetup backgroundColor="255,128,0" bold="false"
                fontName="" fontSize="0" italic="false"
                startIndex="1260" stopIndex="1967" underline="false"/>
        </textRangeSetupList>
    </setupInfo>
    <setupInfo analysisSubsets="Analysis Results (MAIN)" selected="true" type="Auto Load Analysis"/>
</vntiDisplaySetup>
DISPLAY_SETUP_END|
COMMENT     
            VNTSEQSTYLE|1979|3331||0|null|null|null|230,230,230|null
            VNTSEQSTYLE|1260|1967||0|null|null|null|255,128,0|null
FEATURES             Location/Qualifiers
     CDS             complement(3469..4329)
                     /label=AmpR
                     /type="CDS"
     polyA_signal    2053..2281
                     /label=SV40 early polyA signal
                     /type="polyA_signal"
     eukaryotic      4870..238
                     /label=chicken Beta-actin
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
     intron          430..1239
                     /label=intron of chicken Beta-actin
                     /type="intron"
     misc_feature    1260..1967
                     /label=mOrange2
                     /type="misc_feature"
                     /vntifkey="21"
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcagccacca
     1261 tggtgagcaa gggcgaggag aataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     1321 tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     1381 gcccctacga gggctttcag accgctaagc tgaaggtgac caagggtggc cccctgccct
     1441 tcgcctggga catcctgtcc cctcatttca cctacggctc caaggcctac gtgaagcacc
     1501 ccgccgacat ccccgactac ttcaagctgt ccttccccga gggcttcaag tgggagcgcg
     1561 tgatgaacta cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     1621 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtga
     1681 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggtg
     1741 ccctgaaggg caagatcaag atgaggctga agctgaagga cggcggccac tacacctccg
     1801 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg
     1861 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     1921 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtca ggactcagat
     1981 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggat
     2041 ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa
     2101 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     2161 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     2221 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     2281 atcatgtctg gatcaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     2341 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2401 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2461 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2521 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2581 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2641 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2701 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2761 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2821 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2881 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2941 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3001 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3061 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3121 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3181 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3241 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3301 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3361 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3421 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3481 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3541 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3601 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3661 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3721 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3781 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3841 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3901 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3961 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4021 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4081 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4141 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4201 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4261 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4321 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4381 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4441 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     4501 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     4561 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     4621 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     4681 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     4741 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     4801 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     4861 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     4921 cagtgccaag ttgggatctt tgcattggcc cacggctctc aggatgggga tgctcccctt
     4981 cagcacccgg ttccccttgg aaactgatgg tcctggctct gtggcatggc agtggcactg
     5041 tgaggagccc cctaccagca gcacacagtg gggttggcac tgccacgctc cggatgccgc
     5101 gctctgatcc aaccccataa tcaagggaac ccgaattgcc ccatcattgc ccccaccacc
     5161 cccatcctgc cgggccctca caccccacgc tgccttctgg tgacattccc cagcccaaac
     5221 ccacggcttc atggctaccg cggggcattt cccattgccg ccccattatc agctctgcac
     5281 acctcccgct gtacccatgc ctcgtggctg cccttctttg acgtataatc ttctaattaa
     5341 tacccggcct tgtcaaagtg gagcacaaac gttaattaat tccccagcag gcaggtaatt
     5401 aacagtgtga ctcccttttt gctgcgagtg gggctgatac agagagatgt ggcactatgg
     5461 agcccacggg gtcctggcac tgggtgccca cggaggtccc catgtgctgc agtgtcaccg
     5521 cctccgagga gacagtattg tccctgcggt gtccctgcag ctcagctctg tccacagggc
     5581 cacctccagt ttggagggga cacaatgcag ccccgatgca acccatcctc gcagcatccc
     5641 agggacaaag accccactgc aagaccgcac acagggctgg gtcccgctcc cctaatatct
     5701 acagtgcttc ttgcatggcc ccttaatcaa tgcagttaat cagcatgcgc tcatgcaccg
     5761 ctctggagct gcaaagcccc tcgcagcgct gctcaccaac accgcgcacc gccccggccc
     5821 agcctgcagc acgcgctgca aacaggaaag aaacaaaata ttgcccaaat gtaggcaaag
     5881 gcattcggct gccttgacct ccgccgggcc gggccctgcc tgactcagct ccttactcag
     5941 cgctcgcttc ctccctccgg ctgccaccgc cgcagcgcac accctgacaa agagtggccc
     6001 ttaacgggct ctgaggtgca cccagcagtg cactcagcag tccaagggcc ggcctggagg
     6061 tttgcaccgc tacgtgctga cattagcatt gaacttggcc ctgggtagtg ctgcaggccg
     6121 ggcggggtgg gtgtagagag tgcagcgcgc gttgcacccg gtgccccttc ccctcccttg
     6181 catcccagca ggctgcaccc cagcaccagg cccgtgcatg catgctcctg gtgttattgc
     6241 agcctggtgc atgcatgcgt cttgatggtg cagcgctgtg catgcatcct ccttggtgtg
     6301 tagcagtcta gtgcatgcat acccctcggt gttattgctg ctctgtgcac gcacgctcat
     6361 tgtatcactt catcccagtg catgcactca cactggagcg attgctgctc ggtgcacgca
     6421 cactcattgt atcactgcag ctcagtggct gcacgcacac cggtgttatt gctgctcggt
     6481 gcgtgcatgc acatcagtgt cgctgcagct cagtgcatgc acgctcattg cccatcgcta
     6541 tccctgcctc tcctgctggc gctccccggg aggtgacttc aaggggaccg caggaccacc
     6601 tcgggggtgg ggggagggct gcacacgcgg accccgctcc ccctccccaa caaagcactg
     6661 tggaatcaaa aaggggggag gggggatgga ggggcgcgtc acacccccgc cccacaccct
     6721 cacctcgagg tgagccccac gttctgcttc actctcccca tctc
//
