LOCUS       pβactin-td-mCherry-Cdk5rap2-CTD        7954 bp    DNA     circular
            
ACCESSION   DNA_pCAG-mcherry-TUBA1B
VERSION     DNA_pCAG-mcherry-TUBA1B.0
KEYWORDS    .

COMMENT
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            <element id="315143959" name="intron of chicken Beta-actin"
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            </element>
            <element id="222449416" target="222449416" text="@L bp"
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                <location x="133" y="336"/>
                <dimension height="300" width="300"/>
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            <element id="1640215324" target="1640215324" text="@N"
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                <location x="84" y="462"/>
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            <element id="1334126563" name="insert_Cdk5rap2-1736-1903"
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    <setupInfo complete="true" completeMinSize="150"
        completeNested="false" includeStopCodon="false"
        incomplete="false" incompleteMinSize="30" selected="false"
        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
        undefinedStart="false" undefinedStop="false" undefinedStopNested="false"/>
    <setupInfo allowBlunt="true" allowFivePrime="true"
        allowThreePrime="true"
        enzymeMap="BamHI|ggatcc,AvaI|cycgrg,KpnI|ggtacc,XhoI|ctcgag,AflII|cttaag,SmaI|cccggg,ApaLI|gtgcac,ApaI|gggccc,NcoI|ccatgg,SalI|gtcgac,ClaI|atcgat,EcoRI|gaattc,NotI|gcggccgc,SacI|gagctc,XmaI|cccggg,AgeI|accggt,BglII|agatct,HindIII|aagctt,BspEI|tccgga,NheI|gctagc,XbaI|tctaga,NdeI|catatg,PstI|ctgcag"
        enzymesCleavePoint="BamHI|2,AvaI|2,KpnI|6,XhoI|2,AflII|2,SmaI|4,ApaLI|2,ApaI|6,NcoI|2,SalI|2,ClaI|3,EcoRI|2,NotI|3,SacI|6,XmaI|2,AgeI|2,BglII|2,HindIII|2,BspEI|2,NheI|2,XbaI|2,NdeI|3,PstI|6"
        enzymesComplCleavePoint="BamHI|0,AvaI|0,KpnI|0,XhoI|0,AflII|0,SmaI|0,ApaLI|0,ApaI|0,NcoI|0,SalI|0,ClaI|0,EcoRI|0,NotI|0,SacI|0,XmaI|0,AgeI|0,BglII|0,HindIII|0,BspEI|0,NheI|0,XbaI|0,NdeI|0,PstI|0"
        enzymesPalindromic="BamHI|true,AvaI|true,KpnI|true,XhoI|true,AflII|true,SmaI|true,ApaLI|true,ApaI|true,NcoI|true,SalI|true,ClaI|true,EcoRI|true,NotI|true,SacI|true,XmaI|true,AgeI|true,BglII|true,HindIII|true,BspEI|true,NheI|true,XbaI|true,NdeI|true,PstI|true"
        ignoreIn="-1,-1" ignoreLess="-1" ignoreMore="-1"
        ignoreOut="-1,-1" selected="true" startIndex="1" type="Restriction Map"/>
    <setupInfo featureList="" selected="true" type="Feature Map"/>
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        textUnderline="false" trackSeparatorColor="128,128,128"
        trackSeparatorWidth="2" type="Sequence"
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                startIndex="2001" stopIndex="2683" underline="false"/>
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                fontName="" fontSize="0" italic="false"
                startIndex="1986" stopIndex="2000" underline="false"/>
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    <setupInfo analysisSubsets="Analysis Results (MAIN)" selected="true" type="Auto Load Analysis"/>
</vntiDisplaySetup>
DISPLAY_SETUP_END|
COMMENT     
            VNTSEQSTYLE|2684|3349||0|null|null|null|230,230,230|null
            VNTSEQSTYLE|1269|1985||0|null|null|null|255,0,0|null
            VNTSEQSTYLE|1253|1268||0|null|null|null|255,0,0|null
            VNTSEQSTYLE|2001|2683||0|null|null|null|255,0,0|null
            VNTSEQSTYLE|1986|2000||0|null|null|null|255,0,0|null
FEATURES             Location/Qualifiers
     CDS             complement(4659..5519)
                     /label=AmpR
                     /type="CDS"
     polyA_signal    3243..3471
                     /label=SV40 early polyA signal
                     /type="polyA_signal"
     eukaryotic      6060..238
                     /label=chicken Beta-actin
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
     intron          430..1239
                     /label=intron of chicken Beta-actin
                     /type="intron"
     CDS             1253..2683
                     /label=td-mCherry
                     /type="CDS"
                     /vntifkey="4"
     misc_feature    2684..3202
                     /label=insert_Cdk5rap2-1736-1903
                     /type="misc_feature"
                     /vntifkey="21"
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcatggtcag
     1261 caaaggggaa gaggataaca tggccatcat caaagagttc atgcggttca aggtgcacat
     1321 ggaaggcagc gtgaacggac atgagttcga gatcgaagga gaaggagagg gacgccctta
     1381 cgagggaact cagaccgcaa agctgaaagt gactaagggt ggccctctgc cgtttgcgtg
     1441 ggacattctg tccccgcaat tcatgtatgg ctcaaaagcc tacgtcaaac acccagctga
     1501 catcccagac tacctgaaac tcagctttcc ggaaggattc aagtgggaac gcgtcatgaa
     1561 tttcgaagat ggcggagtgg tcaccgtgac tcaagacagc tcgttgcaag acggggagtt
     1621 tatctacaag gtgaagctcc ggggaactaa cttcccatca gacggtccgg tcatgcagaa
     1681 aaagacgatg ggttgggaag cctcctcgga gagaatgtac ccagaggacg gcgcgctgaa
     1741 gggtgaaatc aaacagagac tcaaactgaa agatggagga cactacgacg cggaagtcaa
     1801 gaccacctac aaggcaaaga aaccagtgca gttgccagga gcgtacaacg tgaacatcaa
     1861 acttgatatc acctcgcaca acgaagatta caccattgtg gagcaatacg aacgggcgga
     1921 aggaaggcat tcgaccgggg gaatggacga attatacaag ggctccggga gcggaatggt
     1981 gagcaaggga gaagaagata acatggctat tatcaaggaa tttatgcgct tcaaggttca
     2041 tatggagggc tcagtgaacg ggcacgaatt tgaaatcgag ggagagggag aaggcagacc
     2101 gtacgaaggc acccagactg ctaagctgaa ggttactaag ggaggacctc tgcccttcgc
     2161 atgggacatc ctttcgccgc agttcatgta cggttccaag gcttatgtga agcacccggc
     2221 agacattccg gattacctga agctgtcatt ccctgagggc tttaagtggg agcgcgtgat
     2281 gaactttgaa gatggtggag tggtgactgt gacccaagac tcctccctgc aagatgggga
     2341 gttcatctac aaagtcaagt tgcgcggaac taatttccct tcggacgggc cggtgatgca
     2401 gaagaaaact atgggatggg aagcttcatc ggaaaggatg taccccgagg acggagccct
     2461 gaaaggggag atcaagcagc ggctcaagct caaggatggc ggtcattacg acgccgaagt
     2521 caagaccacg tacaaggcca agaagccggt gcagctccct ggcgcgtaca atgtcaatat
     2581 caagctcgac atcacgtccc ataatgagga ttatactatc gtggaacagt acgagcgcgc
     2641 cgaaggacgg cactcaaccg gaggaatgga cgagctgtac aagagatctc acatgctgtg
     2701 cctgattgag gactatgatg ccctctataa gcagatcagc tggggccaga cactgcttgc
     2761 caagatggat attcaaaccc aagaggctct gagccccaca agtcagaagc tgggaccaaa
     2821 ggcttcattc tctgtgcctc tgagcaagtt tctctccagc atgaacacag ccaagctgat
     2881 cctggaaaaa gcctccaggt tgctgaagct cttctggagg gtctctgtcc ccaccaacgg
     2941 ccagtgttcc cttcactgtg accagattgg agaaatgaag gcagagatca ccaaactaca
     3001 caaaaaattg tttgaacaag aaaagaagct gcagaacaca gcaaaacttc tgcagcagag
     3061 caagcaccag gagaaaatca tctttgatca gttggtcatc acccaccaag tccttcggaa
     3121 agccagggga aacctggagc tcaggcctag agctgcccat ccaggaacat ccagtcccag
     3181 cagaccgggc tcatgagtcg acggtaccgc gggcccggga tccaccggat ctagataagt
     3241 aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc
     3301 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     3361 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3421 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3481 gatcaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3541 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3601 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3661 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3721 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3781 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3841 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3901 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3961 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4021 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4081 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4141 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4201 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4261 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4321 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4381 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4441 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4501 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4561 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4621 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4681 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4741 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4801 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4861 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4921 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4981 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5041 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5101 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5161 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5221 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5281 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5341 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5401 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5461 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5521 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5581 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5641 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     5701 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     5761 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     5821 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     5881 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     5941 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     6001 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     6061 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
     6121 ttgggatctt tgcattggcc cacggctctc aggatgggga tgctcccctt cagcacccgg
     6181 ttccccttgg aaactgatgg tcctggctct gtggcatggc agtggcactg tgaggagccc
     6241 cctaccagca gcacacagtg gggttggcac tgccacgctc cggatgccgc gctctgatcc
     6301 aaccccataa tcaagggaac ccgaattgcc ccatcattgc ccccaccacc cccatcctgc
     6361 cgggccctca caccccacgc tgccttctgg tgacattccc cagcccaaac ccacggcttc
     6421 atggctaccg cggggcattt cccattgccg ccccattatc agctctgcac acctcccgct
     6481 gtacccatgc ctcgtggctg cccttctttg acgtataatc ttctaattaa tacccggcct
     6541 tgtcaaagtg gagcacaaac gttaattaat tccccagcag gcaggtaatt aacagtgtga
     6601 ctcccttttt gctgcgagtg gggctgatac agagagatgt ggcactatgg agcccacggg
     6661 gtcctggcac tgggtgccca cggaggtccc catgtgctgc agtgtcaccg cctccgagga
     6721 gacagtattg tccctgcggt gtccctgcag ctcagctctg tccacagggc cacctccagt
     6781 ttggagggga cacaatgcag ccccgatgca acccatcctc gcagcatccc agggacaaag
     6841 accccactgc aagaccgcac acagggctgg gtcccgctcc cctaatatct acagtgcttc
     6901 ttgcatggcc ccttaatcaa tgcagttaat cagcatgcgc tcatgcaccg ctctggagct
     6961 gcaaagcccc tcgcagcgct gctcaccaac accgcgcacc gccccggccc agcctgcagc
     7021 acgcgctgca aacaggaaag aaacaaaata ttgcccaaat gtaggcaaag gcattcggct
     7081 gccttgacct ccgccgggcc gggccctgcc tgactcagct ccttactcag cgctcgcttc
     7141 ctccctccgg ctgccaccgc cgcagcgcac accctgacaa agagtggccc ttaacgggct
     7201 ctgaggtgca cccagcagtg cactcagcag tccaagggcc ggcctggagg tttgcaccgc
     7261 tacgtgctga cattagcatt gaacttggcc ctgggtagtg ctgcaggccg ggcggggtgg
     7321 gtgtagagag tgcagcgcgc gttgcacccg gtgccccttc ccctcccttg catcccagca
     7381 ggctgcaccc cagcaccagg cccgtgcatg catgctcctg gtgttattgc agcctggtgc
     7441 atgcatgcgt cttgatggtg cagcgctgtg catgcatcct ccttggtgtg tagcagtcta
     7501 gtgcatgcat acccctcggt gttattgctg ctctgtgcac gcacgctcat tgtatcactt
     7561 catcccagtg catgcactca cactggagcg attgctgctc ggtgcacgca cactcattgt
     7621 atcactgcag ctcagtggct gcacgcacac cggtgttatt gctgctcggt gcgtgcatgc
     7681 acatcagtgt cgctgcagct cagtgcatgc acgctcattg cccatcgcta tccctgcctc
     7741 tcctgctggc gctccccggg aggtgacttc aaggggaccg caggaccacc tcgggggtgg
     7801 ggggagggct gcacacgcgg accccgctcc ccctccccaa caaagcactg tggaatcaaa
     7861 aaggggggag gggggatgga ggggcgcgtc acacccccgc cccacaccct cacctcgagg
     7921 tgagccccac gttctgcttc actctcccca tctc
//
