LOCUS       pβactin-Nedd1-mOrange2        8821 bp    DNA     circular
            
ACCESSION   DNA_pCAG-mcherry-TUBA1B
VERSION     DNA_pCAG-mcherry-TUBA1B.0
KEYWORDS    .

COMMENT
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COMMENT     
            VNTSEQSTYLE|1268|1276||0|null|null|null|255,0,128|null
            VNTSEQSTYLE|1976|2629||0|null|null|null|255,0,0|null
            VNTSEQSTYLE|1277|1975||0|null|null|null|255,0,0|null
            VNTSEQSTYLE|2651|2686||0|null|null|null|255,0,0|null
            VNTSEQSTYLE|2687|2686||0|null|null|null|255,0,0|null
            VNTSEQSTYLE|3254|3310||0|null|null|null|255,255,255|null
            VNTSEQSTYLE|2630|2650||0|null|null|null|255,0,0|null
            VNTSEQSTYLE|2687|3253||0|null|null|null|255,0,0|null
            VNTSEQSTYLE|3311|3310||0|null|null|null|0,255,0|null
            VNTSEQSTYLE|3311|4018||0|null|null|null|255,127,0|null
FEATURES             Location/Qualifiers
     CDS             complement(5526..6386)
                     /label=AmpR
                     /type="CDS"
     polyA_signal    4110..4338
                     /label=SV40 early polyA signal
                     /type="polyA_signal"
     eukaryotic      6927..238
                     /label=chicken beta-actin promotor
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
     intron          430..1239
                     /label=intron of chicken beta-actin
                     /type="intron"
     CDS             1277..3253
                     /label=rNedd1
                     /type="CDS"
                     /vntifkey="4"
     CDS             3311..4018
                     /label=mOrange2
                     /type="CDS"
                     /vntifkey="4"
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcctcgagaa
     1261 ttctgcctga ggagcgatgc aggaaaacct cagatttgcc tcatcaggag atgatgttaa
     1321 aatatgggat gcttcgtttc tgacattggt ggataaattc aacccccaca cgtcaccgca
     1381 tggaatcagt tcaatctgtt ggagcagcaa taataacttc ctagtgactg cgtcttctgg
     1441 tggtgacaaa atagtggtgt caagttgcaa atgtaagccc gttccacttt tggagcttgc
     1501 ggaagggcaa aagcagacat gtgtcgattt aaattctaca tcaatgtatt tggcaagtgg
     1561 aggcctaaat aacactgtta atatttggga tttaaagtca aaacgacttc atcggtctct
     1621 taaggaccat aaacgggaag taacttgtgt ggcgtacaat tggaatgatt gttacattgc
     1681 ttccgggtct ctcagtggtg agattatctt acacagcgta accactaata cctccagtac
     1741 gcctttcggc catgggagta agcagcctgt ccggcacatc aagtactcgt tgtttaggaa
     1801 gtctctcttg ggcagcgttt cggataatgg agtagtgacc ctctgggatg tgaacagtca
     1861 gagtgcatac cacagctttg acagtacaca caaagcccca gcttcaggcc tctgcttctc
     1921 tcctgtcaac gaactgctgt ttgtgaccat aggcctggat aaaagaatca tcctgtatga
     1981 cacttcaagt aagaagctgg tgaaaacttt agtggctgac actcctctga ctgctgtaga
     2041 ttttatgcca gatggagcca gtttggctat tggatcctcc cgtgggaaaa tatatcagta
     2101 tgacttaagg atgctgaaat ctcccgtgaa aaccatcagc gcccacaaga catctgtgca
     2161 gtgtatagca tttcagtact ccacatctct gacgaaggca agtttaaata aaggctgctc
     2221 aaataaggcc acagcagcga acaaacggag tgttcctgtg ggcagcagca gtggggctgc
     2281 ccagaattct ggaattgtca gagaagcacc cagcccttcc attgccacag ttctcccaca
     2341 gcccgtgacg acagctgtgg gcaaaggagc aggggctgct caggacgaag caggtttgcc
     2401 tcgaagcaaa aatacagata tcttatctaa ggaaactgat gctggaaaaa gtcaagactt
     2461 ctccagcttt gatgacacgg ggaagagtag tttaggggac atgttttcac caattagaga
     2521 tgatgctgta gtcaacaaag gaggtgacga gtctgtaggc aaaggagatg gccttgactt
     2581 tctaccacag ctaaactcgg tgttccccct gaggaagaat gcaggctcct ccagcactct
     2641 ggtgctgcac tccagccctt ttaatgtcct tatggggaca gggaaggaag agcatgagag
     2701 tcacgagctg tcagccgagt ctaagaaggc acaccttggg aagccggagc ctaaggactc
     2761 cttgaagcag tttacaaagt tgatctcctc tggtgctgaa cctggaattc taaacacctg
     2821 tccatcatct aaccaagcaa gaaatcttga aaagtttgaa aagccagaaa aagatattga
     2881 agtccagttg atgcacgaac cctcagtcaa tggatcctca actccagtac caaaggcagc
     2941 ctcctccgtc actgctggag tagccagttc actctcagaa aaaatagtcg acacccttgg
     3001 gaacagtcgg ccggccgcgc cacttacctc tgtccagatc cgcttcattc agaacatgat
     3061 acaagaaacc ctggatgact tcagggaagc ttgccatagg gacattgtga atctgcaagt
     3121 ggaaatgatt aaacagtttc atatgcaact gaatgaaatg cactctttgc tggaaagata
     3181 ctcagtgaat gaaggcttag tggctgagat cgaaaggctg cgagaagaaa acaaaaggct
     3241 gcgggcccac ttcctcgacg gcagcaagct tggtggaggc agcgcatcag gcggaagcgg
     3301 aagcagatct atggtgagca agggcgagga gaataacatg gccatcatca aggagttcat
     3361 gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     3421 gggcgagggc cgcccctacg agggctttca gaccgctaag ctgaaggtga ccaagggtgg
     3481 ccccctgccc ttcgcctggg acatcctgtc ccctcatttc acctacggct ccaaggccta
     3541 cgtgaagcac cccgccgaca tccccgacta cttcaagctg tccttccccg agggcttcaa
     3601 gtgggagcgc gtgatgaact acgaggacgg cggcgtggtg accgtgaccc aggactcctc
     3661 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     3721 cggccccgtg atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     3781 cgaggacggt gccctgaagg gcaagatcaa gatgaggctg aagctgaagg acggcggcca
     3841 ctacacctcc gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     3901 ctacatcgtc gacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     3961 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     4021 agcggccgcc atatgcatcc taggcctatt aatattccgg agtatacgta gccggctaac
     4081 gttaacaacc ggtacctcta gataagtaat gatcataatc agccatatca catctgtaga
     4141 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     4201 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4261 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4321 actcatcaat gtatcttatc atgtctggat caattcgtaa tcatgtcata gctgtttcct
     4381 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4441 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4501 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4561 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4621 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4681 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4741 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4801 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4861 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4921 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4981 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5041 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5101 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5161 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5221 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5281 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5341 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5401 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5461 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5521 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5581 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5641 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5701 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5761 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5821 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5881 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5941 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6001 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6061 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6121 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6181 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6241 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6301 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6361 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6421 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6481 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     6541 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     6601 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     6661 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     6721 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     6781 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     6841 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     6901 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     6961 cgttgtaaaa cgacggccag tgccaagttg ggatctttgc attggcccac ggctctcagg
     7021 atggggatgc tccccttcag cacccggttc cccttggaaa ctgatggtcc tggctctgtg
     7081 gcatggcagt ggcactgtga ggagccccct accagcagca cacagtgggg ttggcactgc
     7141 cacgctccgg atgccgcgct ctgatccaac cccataatca agggaacccg aattgcccca
     7201 tcattgcccc caccaccccc atcctgccgg gccctcacac cccacgctgc cttctggtga
     7261 cattccccag cccaaaccca cggcttcatg gctaccgcgg ggcatttccc attgccgccc
     7321 cattatcagc tctgcacacc tcccgctgta cccatgcctc gtggctgccc ttctttgacg
     7381 tataatcttc taattaatac ccggccttgt caaagtggag cacaaacgtt aattaattcc
     7441 ccagcaggca ggtaattaac agtgtgactc cctttttgct gcgagtgggg ctgatacaga
     7501 gagatgtggc actatggagc ccacggggtc ctggcactgg gtgcccacgg aggtccccat
     7561 gtgctgcagt gtcaccgcct ccgaggagac agtattgtcc ctgcggtgtc cctgcagctc
     7621 agctctgtcc acagggccac ctccagtttg gaggggacac aatgcagccc cgatgcaacc
     7681 catcctcgca gcatcccagg gacaaagacc ccactgcaag accgcacaca gggctgggtc
     7741 ccgctcccct aatatctaca gtgcttcttg catggcccct taatcaatgc agttaatcag
     7801 catgcgctca tgcaccgctc tggagctgca aagcccctcg cagcgctgct caccaacacc
     7861 gcgcaccgcc ccggcccagc ctgcagcacg cgctgcaaac aggaaagaaa caaaatattg
     7921 cccaaatgta ggcaaaggca ttcggctgcc ttgacctccg ccgggccggg ccctgcctga
     7981 ctcagctcct tactcagcgc tcgcttcctc cctccggctg ccaccgccgc agcgcacacc
     8041 ctgacaaaga gtggccctta acgggctctg aggtgcaccc agcagtgcac tcagcagtcc
     8101 aagggccggc ctggaggttt gcaccgctac gtgctgacat tagcattgaa cttggccctg
     8161 ggtagtgctg caggccgggc ggggtgggtg tagagagtgc agcgcgcgtt gcacccggtg
     8221 ccccttcccc tcccttgcat cccagcaggc tgcaccccag caccaggccc gtgcatgcat
     8281 gctcctggtg ttattgcagc ctggtgcatg catgcgtctt gatggtgcag cgctgtgcat
     8341 gcatcctcct tggtgtgtag cagtctagtg catgcatacc cctcggtgtt attgctgctc
     8401 tgtgcacgca cgctcattgt atcacttcat cccagtgcat gcactcacac tggagcgatt
     8461 gctgctcggt gcacgcacac tcattgtatc actgcagctc agtggctgca cgcacaccgg
     8521 tgttattgct gctcggtgcg tgcatgcaca tcagtgtcgc tgcagctcag tgcatgcacg
     8581 ctcattgccc atcgctatcc ctgcctctcc tgctggcgct ccccgggagg tgacttcaag
     8641 gggaccgcag gaccacctcg ggggtggggg gagggctgca cacgcggacc ccgctccccc
     8701 tccccaacaa agcactgtgg aatcaaaaag gggggagggg ggatggaggg gcgcgtcaca
     8761 cccccgcccc acaccctcac ctcgaggtga gccccacgtt ctgcttcact ctccccatct
     8821 c
//
