LOCUS       pβactin-AcGFP-128-425-Akap9        7661 bp    DNA     circular
            
ACCESSION   DNA_pCAG-mcherry-TUBA1B
VERSION     DNA_pCAG-mcherry-TUBA1B.0
KEYWORDS    .

COMMENT
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                <location x="147" y="255"/>
                <dimension height="412" width="412"/>
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            <element id="901053131" name="AcGFP"
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                <location x="553" y="402"/>
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            <element id="901053131" name="AcGFP" shape="1"
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                <location x="147" y="255"/>
                <dimension height="412" width="412"/>
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            <element id="1611340213" name="intron of chicken Beta-actin"
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                <location x="481" y="290"/>
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            <element id="1611340213" name="intron of chicken Beta-actin"
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            <element id="1611340213" name="intron of chicken Beta-actin"
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                target="intron of chicken Beta-actin:430 - 1239"
                type="15" xsi:type="symbol">
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                    <fill pattern="Solid">
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                <location x="172" y="280"/>
                <dimension height="362" width="362"/>
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            <element name="Intron" type="15" xsi:type="annotation">
                <label text="">
                    <style name="Signal Label" xsi:type="basicStyle">
                        <label text="@N">
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                <connector>
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            <element id="1822088930" name="chicken Beta-actin"
                target="chicken Beta-actin:5767 - 238" text="@N"
                type="29" xsi:type="label">
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                <location x="145" y="289"/>
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            <element id="1822088930" name="chicken Beta-actin"
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            <element id="1822088930" name="chicken Beta-actin" shape="1"
                target="chicken Beta-actin:5767 - 238" type="29" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
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                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
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                </style>
                <location x="172" y="280"/>
                <dimension height="362" width="362"/>
            </element>
            <element id="1843394292" target="1843394292" text="@L bp"
                type="-2055714368" xsi:type="label">
                <style name="Molecule Label 2" xsi:type="basicStyle">
                    <label text="@L bp">
                        <font name="Arial" size="18" style="0"/>
                        <color blue="50" green="50" red="50"/>
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                </style>
                <location x="323" y="465"/>
            </element>
            <element id="1079449623" target="2055105" type="2055105" xsi:type="symbol">
                <style name="Axis" xsi:type="basicStyle">
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                </style>
                <location x="203" y="311"/>
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            </element>
            <element id="2121543737" target="2121543737" text="@N"
                type="346519525" xsi:type="label">
                <style name="Molecule Label 1" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="24" style="1"/>
                        <color blue="50" green="50" red="50"/>
                    </label>
                </style>
                <location x="178" y="437"/>
            </element>
            <element id="2068170218"
                name="insert_AcGFP-rAkap9-128-425-NheI-SalI"
                target="insert_AcGFP-rAkap9-128-425-NheI-SalI:1247 - 2909"
                text="@N" type="21" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
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                </style>
                <location x="557" y="487"/>
            </element>
            <element id="2068170218"
                name="insert_AcGFP-rAkap9-128-425-NheI-SalI"
                target="insert_AcGFP-rAkap9-128-425-NheI-SalI:1247 - 2909"
                type="21" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
                    <line width="1">
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            </element>
            <element id="2068170218"
                name="insert_AcGFP-rAkap9-128-425-NheI-SalI" shape="1"
                target="insert_AcGFP-rAkap9-128-425-NheI-SalI:1247 - 2909"
                type="21" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
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                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="172" y="280"/>
                <dimension height="362" width="362"/>
            </element>
            <element id="1859133457" name="sequenced by AcGFP-2-FP"
                target="sequenced by AcGFP-2-FP:2910 - 3450" text="@N"
                type="21" xsi:type="label">
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                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
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                <location x="456" y="638"/>
            </element>
            <element id="1859133457" name="sequenced by AcGFP-2-FP"
                target="sequenced by AcGFP-2-FP:2910 - 3450" type="21" xsi:type="line">
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            <element id="1859133457" name="sequenced by AcGFP-2-FP"
                shape="1" target="sequenced by AcGFP-2-FP:2910 - 3450"
                type="21" xsi:type="symbol">
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                <dimension height="362" width="362"/>
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    </setupInfo>
    <setupInfo complete="true" completeMinSize="150"
        completeNested="false" includeStopCodon="false"
        incomplete="false" incompleteMinSize="30" selected="false"
        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
        undefinedStart="false" undefinedStop="false" undefinedStopNested="false"/>
    <setupInfo allowBlunt="true" allowFivePrime="true"
        allowThreePrime="true"
        enzymeMap="BamHI|ggatcc,AvaI|cycgrg,KpnI|ggtacc,XhoI|ctcgag,AflII|cttaag,SmaI|cccggg,ApaLI|gtgcac,ApaI|gggccc,NcoI|ccatgg,SalI|gtcgac,ClaI|atcgat,EcoRI|gaattc,NotI|gcggccgc,SacI|gagctc,XmaI|cccggg,AgeI|accggt,BglII|agatct,HindIII|aagctt,BspEI|tccgga,NheI|gctagc,XbaI|tctaga,NdeI|catatg,PstI|ctgcag"
        enzymesCleavePoint="BamHI|2,AvaI|2,KpnI|6,XhoI|2,AflII|2,SmaI|4,ApaLI|2,ApaI|6,NcoI|2,SalI|2,ClaI|3,EcoRI|2,NotI|3,SacI|6,XmaI|2,AgeI|2,BglII|2,HindIII|2,BspEI|2,NheI|2,XbaI|2,NdeI|3,PstI|6"
        enzymesComplCleavePoint="BamHI|0,AvaI|0,KpnI|0,XhoI|0,AflII|0,SmaI|0,ApaLI|0,ApaI|0,NcoI|0,SalI|0,ClaI|0,EcoRI|0,NotI|0,SacI|0,XmaI|0,AgeI|0,BglII|0,HindIII|0,BspEI|0,NheI|0,XbaI|0,NdeI|0,PstI|0"
        enzymesPalindromic="BamHI|true,AvaI|true,KpnI|true,XhoI|true,AflII|true,SmaI|true,ApaLI|true,ApaI|true,NcoI|true,SalI|true,ClaI|true,EcoRI|true,NotI|true,SacI|true,XmaI|true,AgeI|true,BglII|true,HindIII|true,BspEI|true,NheI|true,XbaI|true,NdeI|true,PstI|true"
        ignoreIn="-1,-1" ignoreLess="-1" ignoreMore="-1"
        ignoreOut="-1,-1" selected="true" startIndex="1" type="Restriction Map"/>
    <setupInfo featureList="" selected="true" type="Feature Map"/>
    <setupInfo basesPerBlock="10" blocksPerLine="10"
        prefer1LetterAACode="false" preferSingleStrand="false"
        selected="true" textBold="false" textColor="0,0,0"
        textFontName="Andale Mono" textFontSize="14" textItalic="false"
        textUnderline="false" trackSeparatorColor="128,128,128"
        trackSeparatorWidth="2" type="Sequence"
        xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:type="sequenceSetup">
        <textRangeSetupList>
            <textRangeSetup backgroundColor="230,230,230" bold="false"
                fontName="" fontSize="0" italic="false"
                startIndex="2910" stopIndex="4228" underline="false"/>
            <textRangeSetup backgroundColor="102,255,102" bold="false"
                fontName="" fontSize="0" italic="false"
                startIndex="1269" stopIndex="1985" underline="false"/>
            <textRangeSetup backgroundColor="255,146,0" bold="false"
                fontName="" fontSize="0" italic="false"
                startIndex="2004" stopIndex="2900" underline="false"/>
        </textRangeSetupList>
    </setupInfo>
    <setupInfo analysisSubsets="Analysis Results (MAIN)" selected="true" type="Auto Load Analysis"/>
</vntiDisplaySetup>
DISPLAY_SETUP_END|
COMMENT     
            VNTSEQSTYLE|2910|4228||0|null|null|null|230,230,230|null
            VNTSEQSTYLE|1269|1985||0|null|null|null|102,255,102|null
            VNTSEQSTYLE|2004|2900||0|null|null|null|255,146,0|null
FEATURES             Location/Qualifiers
     CDS             complement(4366..5226)
                     /label=AmpR
                     /type="CDS"
     polyA_signal    2950..3178
                     /label=SV40 early polyA signal
                     /type="polyA_signal"
     misc_feature    2910..3450
                     /label=sequenced by AcGFP-2-FP
                     /type="misc_feature"
     eukaryotic      5767..238
                     /label=chicken Beta-actin
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
     intron          430..1239
                     /label=intron of chicken Beta-actin
                     /type="intron"
     misc_feature    1247..2909
                     /label=insert_AcGFP-rAkap9-128-425-NheI-SalI
                     /type="misc_feature"
     CDS             1269..1985
                     /label=AcGFP
                     /type="CDS"
     CDS             2004..2900
                     /label=rAkap9-128-425
                     /type="CDS"
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcgctaccgg
     1261 tcgccaccat ggtgagcaag ggcgccgagc tgttcaccgg catcgtgccc atcctgatcg
     1321 agctgaatgg cgatgtgaat ggccacaagt tcagcgtgag cggcgagggc gagggcgatg
     1381 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cctgtgccct
     1441 ggcccaccct ggtgaccacc ctgagctacg gcgtgcagtg cttctcacgc taccccgatc
     1501 acatgaagca gcacgacttc ttcaagagcg ccatgcctga gggctacatc caggagcgca
     1561 ccatcttctt cgaggatgac ggcaactaca agtcgcgcgc cgaggtgaag ttcgagggcg
     1621 ataccctggt gaatcgcatc gagctgaccg gcaccgattt caaggaggat ggcaacatcc
     1681 tgggcaataa gatggagtac aactacaacg cccacaatgt gtacatcatg accgacaagg
     1741 ccaagaatgg catcaaggtg aacttcaaga tccgccacaa catcgaggat ggcagcgtgc
     1801 agctggccga ccactaccag cagaataccc ccatcggcga tggccctgtg ctgctgcccg
     1861 ataaccacta cctgtccacc cagagcgccc tgtccaagga ccccaacgag aagcgcgatc
     1921 acatgatcta cttcggcttc gtgaccgccg ccgccatcac ccacggcatg gatgagctgt
     1981 acaagtccgg aggtggtgga ggtatggggg aaaacgagtt ggctgagaag cagcatgagg
     2041 tcgaagagtt gactcaggag ttggaagaaa tgagggccag ctttgggacg gaagggctga
     2101 agcagttaca agaattcgaa gccgccatta aacaaagaga tggcatcatc actcagctca
     2161 ctgctaactt gcagcaagcc agaaaagaaa aggatgacac aacgatagaa tttttagagt
     2221 tgacagaaca gagtcaaaag ctgcagattc agttccagca cttacaggct aatgaaaccc
     2281 tgcaaaacag cactcttagt cgaactgcaa cagatttact gcaggctaaa cgacagatct
     2341 ttactctgca gcaacagctg caagattatc agaaaaagga ggaggacgtc caagcacaaa
     2401 tttctttttt acaagagaaa attagaatgt tggaaatgga aaaagacaga aaagtggaaa
     2461 gcttaaatac aaaagaaata gaggaaaagc aggcaatcat tgaggagttg aatacaagag
     2521 taatagaaga agagagaaaa actgctgagc tagagaataa agtgacaact gctgatgaat
     2581 tactaggaca gctccaagga caggttacag aaaaggatca ggagatcaaa agcctgaaac
     2641 tagaactggt caactctcag cagaacgagc ggaagtgttc agaggaaatc aaagagctaa
     2701 tgaggacagt tgaagaactc cagaagagaa atctcaagga cagccagctg gggaccggca
     2761 ccgtgcgaag ggtggaacaa gaaacacaac ggaaactaag ccttctccag gcagagctgg
     2821 atgagatgta tgggaagcag atagtgcaaa tgaaacaaga attaatgaaa cagcacatgt
     2881 ctcagataga ggagctttga cgagtcgacg gtaccgcggg cccgggatcc accggatcta
     2941 gataagtaat gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa
     3001 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     3061 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3121 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3181 atgtctggat caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     3241 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3301 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3361 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3421 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3481 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3541 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3601 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3661 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3721 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3781 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3841 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3901 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3961 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4021 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4081 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4141 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4201 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4261 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4321 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4381 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4441 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4501 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4561 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4621 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4681 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4741 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4801 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4861 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4921 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4981 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5041 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5101 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5161 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5221 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5281 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5341 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     5401 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     5461 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     5521 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     5581 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     5641 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     5701 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     5761 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     5821 tgccaagttg ggatctttgc attggcccac ggctctcagg atggggatgc tccccttcag
     5881 cacccggttc cccttggaaa ctgatggtcc tggctctgtg gcatggcagt ggcactgtga
     5941 ggagccccct accagcagca cacagtgggg ttggcactgc cacgctccgg atgccgcgct
     6001 ctgatccaac cccataatca agggaacccg aattgcccca tcattgcccc caccaccccc
     6061 atcctgccgg gccctcacac cccacgctgc cttctggtga cattccccag cccaaaccca
     6121 cggcttcatg gctaccgcgg ggcatttccc attgccgccc cattatcagc tctgcacacc
     6181 tcccgctgta cccatgcctc gtggctgccc ttctttgacg tataatcttc taattaatac
     6241 ccggccttgt caaagtggag cacaaacgtt aattaattcc ccagcaggca ggtaattaac
     6301 agtgtgactc cctttttgct gcgagtgggg ctgatacaga gagatgtggc actatggagc
     6361 ccacggggtc ctggcactgg gtgcccacgg aggtccccat gtgctgcagt gtcaccgcct
     6421 ccgaggagac agtattgtcc ctgcggtgtc cctgcagctc agctctgtcc acagggccac
     6481 ctccagtttg gaggggacac aatgcagccc cgatgcaacc catcctcgca gcatcccagg
     6541 gacaaagacc ccactgcaag accgcacaca gggctgggtc ccgctcccct aatatctaca
     6601 gtgcttcttg catggcccct taatcaatgc agttaatcag catgcgctca tgcaccgctc
     6661 tggagctgca aagcccctcg cagcgctgct caccaacacc gcgcaccgcc ccggcccagc
     6721 ctgcagcacg cgctgcaaac aggaaagaaa caaaatattg cccaaatgta ggcaaaggca
     6781 ttcggctgcc ttgacctccg ccgggccggg ccctgcctga ctcagctcct tactcagcgc
     6841 tcgcttcctc cctccggctg ccaccgccgc agcgcacacc ctgacaaaga gtggccctta
     6901 acgggctctg aggtgcaccc agcagtgcac tcagcagtcc aagggccggc ctggaggttt
     6961 gcaccgctac gtgctgacat tagcattgaa cttggccctg ggtagtgctg caggccgggc
     7021 ggggtgggtg tagagagtgc agcgcgcgtt gcacccggtg ccccttcccc tcccttgcat
     7081 cccagcaggc tgcaccccag caccaggccc gtgcatgcat gctcctggtg ttattgcagc
     7141 ctggtgcatg catgcgtctt gatggtgcag cgctgtgcat gcatcctcct tggtgtgtag
     7201 cagtctagtg catgcatacc cctcggtgtt attgctgctc tgtgcacgca cgctcattgt
     7261 atcacttcat cccagtgcat gcactcacac tggagcgatt gctgctcggt gcacgcacac
     7321 tcattgtatc actgcagctc agtggctgca cgcacaccgg tgttattgct gctcggtgcg
     7381 tgcatgcaca tcagtgtcgc tgcagctcag tgcatgcacg ctcattgccc atcgctatcc
     7441 ctgcctctcc tgctggcgct ccccgggagg tgacttcaag gggaccgcag gaccacctcg
     7501 ggggtggggg gagggctgca cacgcggacc ccgctccccc tccccaacaa agcactgtgg
     7561 aatcaaaaag gggggagggg ggatggaggg gcgcgtcaca cccccgcccc acaccctcac
     7621 ctcgaggtga gccccacgtt ctgcttcact ctccccatct c
//
