LOCUS       pCAG-mScarlet-Gi        5931 bp DNA     circular SYN 30-MAY-2022
DEFINITION  pCAG-EGFP.
ACCESSION   DNA_pCAG-loxP-STOP-loxP-ZsGreen
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     Backbone  is pCAG-EGFP which was linearized with PstI/SacI to 
            release EGFP.mScarlet-Giantin was amplified from 
            pmScarlet-H_Giantin_C1 (Addgene Plasmid #85049) with 
            primers:PstI-Giantin fw 
            5'-ggtcgacgacctgcaggccaccatggtgagcaagg-3'SacI-Giantin rv 
            5'-GGCTGATCAGCGAGCTCCTATAGATGGCCCGTAAAAC-3'aprox. 1200 bpResistance 
            is ampicillin 
            VNTSEQSTYLE|2604|2637||0|false|false|false|null|255,9,18 
            VNTSEQSTYLE|1733|1766||0|false|false|false|null|255,15,31
FEATURES             Location/Qualifiers
     source          1..5931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    86..1536
                     /label=CAG promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     enhancer        86..373
                     /label=CAG enhancer
                     /note="/type=enhancer"
                     /note="/vntifkey=9"
     misc_feature    1728..2834
                     /label=mScarlet-Giantin
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    1728..2423
                     /label=mScarlet
                     /label=nonstandard type: misc_marker
                     /note="/type=misc_marker"
                     /note="/vntifkey=22"
     misc_feature    2445..2834
                     /label=Giantin
                     /note="C-terminal portion (3131-3259) of Homo sapiens
                     golgin B1 (GOLGB1)"
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     polyA_signal    2853..3080
                     /label=bGH polyA_terminator
                     /note="/type=polyA_signal"
                     /note="/vntifkey=25"
     promoter        3826..3854
                     /label=Ampicilin promoter
                     /note="/type=promoter"
                     /note="/vntifkey=30"
     misc_feature    3896..4756
                     /label=Ampicilin resistance
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
      841 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta
     1681 attaataata cgactcacta tagggggtcg acgacctgca ggccaccatg gtgagcaagg
     1741 gcgaggcagt gatcaaggag ttcatgcggt tcaaggtgca catggagggc tccatgaacg
     1801 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     1861 ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc ctgggacatc ctgtcccctc
     1921 agttcatgta cggctccagg gccttcacca agcaccccgc cgacatcccc gactactata
     1981 agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2041 ccgtgaccgt gacccaggac acctccctgg aggacggcac cctgatctac aaggtgaagc
     2101 tccgcggcac caacttccct cctgacggcc ccgtaatgca gaagaagaca atgggctggg
     2161 aagcatccac cgagcggttg taccccgagg acggcgtgct gaagggcgac attaagatgg
     2221 ccctgcgcct gaaggacggc ggccgctacc tggcggactt caagaccacc tacaaggcca
     2281 agaagcccgt gcagatgccc ggcgcctaca acgtcgaccg caagttggac atcacctccc
     2341 acaacgagga ctacaccgtg gtggaacagt acgaacgctc cgagggccgc cactccaccg
     2401 gcggcatgga cgagctgtac aagtccggac tcagatctcg aggagaaccg cagcaaagct
     2461 tttctgaagc tcagcagcag ctatgcaaca ccagacagga agtgaatgaa ttaaggaagc
     2521 tgctggaaga agaacgagac caaagagtgg ctgctgagaa tgctctctct gtggccgagg
     2581 agcagatcag acggttagag cacagtgaat gggactcttc ccggactcct atcattggct
     2641 cctgtggcac tcaggagcag gcactgttaa tagatcttac aagcaacagt tgtcgaagga
     2701 cccggagtgg cgttggatgg aagcgagtcc tgcgttcact ctgtcattca cggacccgag
     2761 tgccacttct agcagccatc tactttctaa tgattcatgt cctgctcatt ctgtgtttta
     2821 cgggccatct ataggagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     2881 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     2941 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     3001 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     3061 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgagatccac
     3121 tagttctagc ctcgaggcta gagcggccgc cactggccgt cgttttacaa cgtcgtgact
     3181 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     3241 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     3301 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3361 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3421 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3481 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3541 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3601 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3661 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3721 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     3781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     3901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4021 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4081 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4141 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4201 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4261 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4321 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4381 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     4441 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4501 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4561 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     4621 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     4681 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4741 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     4801 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4861 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4921 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5041 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     5101 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5161 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5221 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5281 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5341 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5401 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5461 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5521 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     5581 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     5641 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     5701 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     5761 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     5821 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     5881 gagcggataa caatttcaca caggaaacag ctatgaccat gaggcgcgcc g
//
