LOCUS       pCAG-rox-inv[Ta1        8332 bp DNA     circular SYN 20-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     Backbone is pCAG-roxPSTOProxP-Lyn-EGFP
            
            pCAG-roxPSTOProxP-Lyn-EGFP  was diggested with HIndIII/BamHI 
            relesaing the STOP sequence.
            
            Ta1-iCre-pA was amplified from pTub-alpha-iCre  using primers:
            LSL-Rb-globin_pA fw 5'-GCGATGAATAAATGAAAGCTGTCGAGGGATCTTCATAAG-3'
            LSL-Ta1-BamHI rv  5'-TCCGGATCAGCTTGATGGGGATCATTCCGTATTAGGAGGG-3'
            Product was LIC cloned into the vector. 
            HindIII and BamHI sites were eliminated
            Resistance= Ampicilin
FEATURES             Location/Qualifiers
     source          1..8332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    86..1536
                     /label=CAG promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    86..373
                     /label=CAG_enhancer
     CDS             complement(805..1536)
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MEKGTAARRRTKALPAHFLPAGAAPLRPRPLEGVRRRLPDFGSAR
                     FGTKEVPAPSRTITIKGNGCGSPRLDSRRRSGAAAPLPRALPGPRRPRPARHPHLPPPP
                     ARHGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSPR
                     SPPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPPH
                     KGPSRSPLRLSRSHRKETSRH"
     CDS             complement(846..1349)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPGT
                     ASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAAPP
                     HRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRTKGP
                     PGAL"
     promoter        1686..1704
                     /label=T7_promoter
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     misc_feature    1721..1752
                     /label=Rox recombination site
                     /note="/type=misc_structure"
                     /note="/vntifkey=88"
     polyA_site      complement(1775..2223)
                     /label=rabbit_polyA_terminator
     polyA_signal    2106..2161
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    complement(2230..3285)
                     /label=NLS-iCre
     CDS             complement(3259..3279)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3282..3291
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3298..4384)
                     /label=Tubulin-alpha-1
     misc_structure  4417..4448
                     /label=Rox recombination site
                     /note="/type=misc_structure"
                     /note="/vntifkey=88"
     misc_feature    4471..5235
                     /label=Lyn-EGFP
                     /note="EGFP tagged with the membrane target domain of 
                     Lyn-Kinase"
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_signal     4471..4500
                     /label=Membrane targeting motif
                     /note="MGCIKSKRKD peptide from Lym kinase"
                     /note="/type=misc_signal"
                     /note="/vntifkey=87"
     misc_feature    4516..5235
                     /label=EGFP
                     /label=nonstandard type: misc_marker
                     /note="/type=misc_marker"
                     /note="/vntifkey=22"
     misc_feature    complement(5251..5268)
                     /label=BGH_rev_primer
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
     terminator      5254..5481
                     /label=bGH_PA_terminator
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
     CDS             5455..5928
                     /codon_start=1
                     /label=ORF frame 2
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSV
                     TATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD"
     promoter        complement(5553..5569)
                     /label=M13_forward20_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    5562..5710
                     /label=lacZ_a
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(5562..5584)
                     /label=M13_pUC_fwd_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     rep_origin      5728..6034
                     /label=f1_origin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        6227..6255
                     /label=AmpR_promoter
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     gene            6297..7157
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             6297..7157
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7312..7931
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        8240..8269
                     /label=lac_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    8283..8305
                     /label=M13_pUC_rev_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        8304..8322
                     /label=M13_reverse_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
      841 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta
     1681 attaataata cgactcacta tagggggtcg acccgccacc taactttaaa taattggcat
     1741 tatttaaagt tatcgcgatg aataaatgaa agctgtcgag ggatcttcat aagagaagag
     1801 ggacagctat gactgggagt agtcaggaga ggaggaaaaa tctggctagt aaaacatgta
     1861 aggaaaattt tagggatgtt aaagaaaaaa ataacacaaa acaaaatata aaaaaaatct
     1921 aacctcaagt caaggctttt ctatggaata aggaatggac agcagggggc tgtttcatat
     1981 actgatgacc tctttatagc cacctttgtt catggcagcc agcatatggc atatgttgcc
     2041 aaactctaaa ccaaatactc attctgatgt tttaaatgat ttgccctccc atatgtcctt
     2101 ccgagtgaga gacacaaaaa attccaacac actattgcaa tgaaaataaa tttcctttat
     2161 tagccagaag tcagatgctc aaggggcttc atgatgtccc cataattttt ggcagaggga
     2221 aaaagatctt cagtccccat cctcgagcag cctcaccatg gccccagtct cagagtccag
     2281 gtttctgatg tagttcatca caatgttcac attggtccag ccaccagcct gcatgatttc
     2341 agggatggac acaccagccc tggccatgtc cctggcagca cccactctgg cagagtggcc
     2401 agaccaggcc aggtatctct gcccagagtc atccttggca ccatagatca ggcggtgggt
     2461 ggcctcaaag atcccttcca gggcccgggt ggacagttgg gaggtggcag aaggggcagc
     2521 cacaccattc tttctgaccc ggcagaacag gtagttgttg gggtcatcag ccacaccaga
     2581 cacagagatc catctctcca ccagcttggt aacccccagg gacagggcct tctccacacc
     2641 agctgtggac accagggtct tggtcctgcc aatgtggatc agcattctcc caccatcggt
     2701 gcgggagatg tccttcactc tgattctggc aatttcggca atgcgcagca gggtgttgta
     2761 ggcaatgccc aggaaggcca ggttcctgat gtcctggcat ctgtcagagt tctccatcag
     2821 ggatctgact tggtcaaagt cagtgcgttc aaaggccagg gcctgcttgg ctctctcccc
     2881 agcatccaca ttctcctttc tgattctcct catcaccagg gacacagcat tggagtcaga
     2941 agggcgaggc aggccagatc tcctgtgcag catgttgagc tggcccaggt gctgttggat
     3001 ggtcttcaca gccaggcctc tggcttgcag gtacaggagg tagtccctca catcctcagg
     3061 ttcagcaggg aaccatttcc tgttgttcag cttgcaccag gcagcccagg atctgcacac
     3121 agacaggagc atcttccagg tgtgttcaga gaaggcctgc ctgtccctga acatgtccat
     3181 caggttcttc ctgacttcat cagaggtggc atccacaggg agggcaggca ggttttggtg
     3241 cacagtcagc aggttggaga ctttcctctt cttcttgggc accatggtgg cgaattcgtt
     3301 gctgcttcgc ggctgccggg tggatggcgg aggcgaggag gaggtgttgc ttcttacagc
     3361 gcgactctta agcggtcgat gtaagagaac ctgcggcact tgggcggatg cgcctcctta
     3421 tatacagctc ggtcaccatg gggatgggcc aaggcctttt cctgttggtc cagacccctc
     3481 cgtctgccca gagaagccgc gacaggagag tggtgattgg tgcaagcgac atggtatgag
     3541 gtaatctctc cccccttccc cccctcgcta gcatccgtag attttgcatc cttcagacaa
     3601 aggcctcgca aaagcaacta gccgagcatc cattatgggg tggagctggg gaggggtaga
     3661 gagggaagaa agaaaacggg ggttgggtga ttatacggag cttaaacctt aaactgtagt
     3721 taggataatt taaggttttc cccaacactt aatcacagaa tttgtgttga gattctttat
     3781 ggtaaattcc agcttttcat ttatactaca ctaaatttaa atacgttccg tcaggattca
     3841 acatctctgt tcgctttaaa taaagtagca agagctatgg gaatgatggt caaaaaggcg
     3901 tgggtaaatg tttagtttta cagttgtatt attgtggtta ttattattac tattattatt
     3961 aatatttgag caaaattcag aagctcagta tgaatagacc acgcctatga ccctcaacag
     4021 cacccgggag gcagaggcag gaagacttgc agctcaaggt catcataacc gcccttagtg
     4081 gtttatgcct gtaaatccca gcattgggaa gcggaggact gcggttattt tgtacccggc
     4141 ctggatatat agtgtgatat cctgtctcaa aaaccaaaaa caaacaaaga gagagagaga
     4201 gagaaaggaa aaaagagaaa caatatacaa agtagtactt aacgctttac tagagagctt
     4261 gctttcttta gcggtagtgc tctcccatca cccctccccg ctcgccccct tacccgaccc
     4321 tacctcaccc cagagcctgt gttattttgt ccctagaaat gagccatccc tcctaatacg
     4381 gaatgatccc catcaagctg atccggaacc cttaattaac tttaaataat tggcattatt
     4441 taaagttagg cagccgccct gcaggccacc atgggatgta ttaaatcaaa aaggaaagac
     4501 ggggcccggt accatatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4561 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4621 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4681 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     4741 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4801 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4861 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4921 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     4981 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     5041 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5101 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     5161 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5221 gagctgtaca agtaagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     5281 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5341 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5401 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     5461 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgagatcca
     5521 ctagttctag cctcgaggct agagcggccg ccactggccg tcgttttaca acgtcgtgac
     5581 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     5641 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     5701 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5761 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5821 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5881 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5941 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6001 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6061 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6121 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     6181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6301 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6361 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6421 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6481 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6541 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6601 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6661 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6721 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6781 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6841 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6901 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6961 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7021 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7081 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7141 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7201 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7441 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     7501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8101 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8161 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8221 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8281 tgagcggata acaatttcac acaggaaaca gctatgacca tgaggcgcgc cg
//
