LOCUS       pCAG-lox-inv[Ta1        8357 bp DNA     circular SYN 26-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   DNA_AAAA
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     Backbone is pCAG-loxPSTOPloxP-Lyn-EGFPppCAG-loxPSTOPloxP-Lyn-EGFP  
            was diggested with HIndIII/BamHITa1-iCre-pA was amplified from 
            pTub-alpha-1-iCre  using primers:LSL-Rb-globin_pA fw 
            5'-GCGATGAATAAATGAAAGCTGTCGAGGGATCTTCATAAG-3'LSL-Ta1-BamHI rv  
            5'-TCCGGATCAGCTTGATGGGGATCATTCCGTATTAGGAGGG-3'Product was LIC cloned
            into the vector.Resistance= Ampicilin
FEATURES             Location/Qualifiers
     source          1..8357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    86..1536
                     /label=CAG promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    86..373
                     /label=CAG_enhancer
                     /note="/vntifkey=21"
     CDS             complement(805..1536)
                     /codon_start=1
                     /label=ORF frame 3
                     /note="/vntifkey=4"
                     /translation="MEKGTAARRRTKALPAHFLPAGAAPLRPRPLEGVRRRLPDFGSAR
                     FGTKEVPAPSRTITIKGNGCGSPRLDSRRRSGAAAPLPRALPGPRRPRPARHPHLPPPP
                     ARHGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSPR
                     SPPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPPH
                     KGPSRSPLRLSRSHRKETSRH"
     CDS             complement(846..1349)
                     /codon_start=1
                     /label=ORF frame 1
                     /note="/vntifkey=4"
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPGT
                     ASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAAPP
                     HRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRTKGP
                     PGAL"
     promoter        1686..1704
                     /label=T7_promoter
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
                     /note="/vntifkey=30"
     misc_feature    complement(1733..1766)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
                     /note="/vntifkey=21"
     polyA_site      complement(1789..2237)
                     /label=rabbit_polyA_terminator
                     /note="/type=polyA_site"
                     /note="/vntifkey=26"
     misc_feature    complement(2244..3299)
                     /label=NLS-iCre
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(3312..4398)
                     /label=Tub-alpha-1
                     /label=nonstandard type: eukaryotic
                     /note="Tubulin-alpha-1 promoter"
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    complement(4431..4464)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
                     /note="/vntifkey=21"
     misc_feature    4496..5260
                     /label=Lyn-EGFP
                     /note="EGFP tagged with the membrane target domain of 
                     Lyn-Kinase"
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_signal     4496..4525
                     /label=Membrane targeting motif
                     /note="MGCIKSKRKD peptide from Lym kinase"
                     /note="/type=misc_signal"
                     /note="/vntifkey=87"
     misc_feature    4541..5260
                     /label=EGFP
                     /label=nonstandard type: misc_marker
                     /note="/type=misc_marker"
                     /note="/vntifkey=22"
     misc_feature    complement(5276..5293)
                     /label=BGH_rev_primer
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
                     /note="/vntifkey=21"
     terminator      5279..5506
                     /label=bGH_PA_terminator
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
                     /note="/vntifkey=43"
     promoter        complement(5578..5594)
                     /label=M13_forward20_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=30"
     misc_feature    5587..5735
                     /label=lacZ_a
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=21"
     misc_feature    complement(5587..5609)
                     /label=M13_pUC_fwd_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=21"
     rep_origin      5753..6059
                     /label=f1_origin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=33"
     promoter        6252..6280
                     /label=AmpR_promoter
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=30"
     gene            6322..7182
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=60"
     CDS             6322..7182
                     /codon_start=1
                     /label=ORF frame 1
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7337..7956
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="/vntifkey=33"
     promoter        8265..8294
                     /label=lac_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="/vntifkey=30"
     misc_feature    8308..8330
                     /label=M13_pUC_rev_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="/vntifkey=21"
     promoter        8329..8347
                     /label=M13_reverse_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="/vntifkey=30"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
      841 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta
     1681 attaataata cgactcacta tagggggtcg acccgccacc aatcatttaa atataacttc
     1741 gtataatgta tgctatacga agttattcgc gatgaataaa tgaaagctgt cgagggatct
     1801 tcataagaga agagggacag ctatgactgg gagtagtcag gagaggagga aaaatctggc
     1861 tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca caaaacaaaa
     1921 tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat ggacagcagg
     1981 gggctgtttc atatactgat gacctcttta tagccacctt tgttcatggc agccagcata
     2041 tggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa tgatttgccc
     2101 tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt gcaatgaaaa
     2161 taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg tccccataat
     2221 ttttggcaga gggaaaaaga tcttcagtcc ccatcctcga gcagcctcac catggcccca
     2281 gtctcagagt ccaggtttct gatgtagttc atcacaatgt tcacattggt ccagccacca
     2341 gcctgcatga tttcagggat ggacacacca gccctggcca tgtccctggc agcacccact
     2401 ctggcagagt ggccagacca ggccaggtat ctctgcccag agtcatcctt ggcaccatag
     2461 atcaggcggt gggtggcctc aaagatccct tccagggccc gggtggacag ttgggaggtg
     2521 gcagaagggg cagccacacc attctttctg acccggcaga acaggtagtt gttggggtca
     2581 tcagccacac cagacacaga gatccatctc tccaccagct tggtaacccc cagggacagg
     2641 gccttctcca caccagctgt ggacaccagg gtcttggtcc tgccaatgtg gatcagcatt
     2701 ctcccaccat cggtgcggga gatgtccttc actctgattc tggcaatttc ggcaatgcgc
     2761 agcagggtgt tgtaggcaat gcccaggaag gccaggttcc tgatgtcctg gcatctgtca
     2821 gagttctcca tcagggatct gacttggtca aagtcagtgc gttcaaaggc cagggcctgc
     2881 ttggctctct ccccagcatc cacattctcc tttctgattc tcctcatcac cagggacaca
     2941 gcattggagt cagaagggcg aggcaggcca gatctcctgt gcagcatgtt gagctggccc
     3001 aggtgctgtt ggatggtctt cacagccagg cctctggctt gcaggtacag gaggtagtcc
     3061 ctcacatcct caggttcagc agggaaccat ttcctgttgt tcagcttgca ccaggcagcc
     3121 caggatctgc acacagacag gagcatcttc caggtgtgtt cagagaaggc ctgcctgtcc
     3181 ctgaacatgt ccatcaggtt cttcctgact tcatcagagg tggcatccac agggagggca
     3241 ggcaggtttt ggtgcacagt cagcaggttg gagactttcc tcttcttctt gggcaccatg
     3301 gtggcgaatt cgttgctgct tcgcggctgc cgggtggatg gcggaggcga ggaggaggtg
     3361 ttgcttctta cagcgcgact cttaagcggt cgatgtaaga gaacctgcgg cacttgggcg
     3421 gatgcgcctc cttatataca gctcggtcac catggggatg ggccaaggcc ttttcctgtt
     3481 ggtccagacc cctccgtctg cccagagaag ccgcgacagg agagtggtga ttggtgcaag
     3541 cgacatggta tgaggtaatc tctcccccct tccccccctc gctagcatcc gtagattttg
     3601 catccttcag acaaaggcct cgcaaaagca actagccgag catccattat ggggtggagc
     3661 tggggagggg tagagaggga agaaagaaaa cgggggttgg gtgattatac ggagcttaaa
     3721 ccttaaactg tagttaggat aatttaaggt tttccccaac acttaatcac agaatttgtg
     3781 ttgagattct ttatggtaaa ttccagcttt tcatttatac tacactaaat ttaaatacgt
     3841 tccgtcagga ttcaacatct ctgttcgctt taaataaagt agcaagagct atgggaatga
     3901 tggtcaaaaa ggcgtgggta aatgtttagt tttacagttg tattattgtg gttattatta
     3961 ttactattat tattaatatt tgagcaaaat tcagaagctc agtatgaata gaccacgcct
     4021 atgaccctca acagcacccg ggaggcagag gcaggaagac ttgcagctca aggtcatcat
     4081 aaccgccctt agtggtttat gcctgtaaat cccagcattg ggaagcggag gactgcggtt
     4141 attttgtacc cggcctggat atatagtgtg atatcctgtc tcaaaaacca aaaacaaaca
     4201 aagagagaga gagagagaaa ggaaaaaaga gaaacaatat acaaagtagt acttaacgct
     4261 ttactagaga gcttgctttc tttagcggta gtgctctccc atcacccctc cccgctcgcc
     4321 cccttacccg accctacctc accccagagc ctgtgttatt ttgtccctag aaatgagcca
     4381 tccctcctaa tacggaatga tccccatcaa gctgatccgg aacccttaat ataacttcgt
     4441 ataatgtatg ctatacgaag ttattaggtc cctcgacctg cagctgcagg ccaccatggg
     4501 atgtattaaa tcaaaaagga aagacggggc ccggtaccat atggtgagca agggcgagga
     4561 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     4621 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4681 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     4741 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4801 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4861 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4921 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4981 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     5041 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     5101 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     5161 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5221 cgccgggatc actctcggca tggacgagct gtacaagtaa gagctcgctg atcagcctcg
     5281 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5341 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5401 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5461 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5521 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgccact
     5581 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5641 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     5701 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     5761 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5821 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5881 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5941 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6001 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6061 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6121 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     6181 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6241 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6301 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6361 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6421 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6481 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6541 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6601 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6661 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6721 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6781 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6841 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6901 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6961 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7021 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7081 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7141 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7201 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7261 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7321 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7381 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7441 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     7501 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7561 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7621 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7681 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7741 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7801 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7861 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7921 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7981 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8041 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8101 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8161 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8221 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8281 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     8341 gaccatgagg cgcgccg
//
