LOCUS       pCAG-lox-inv[Ta1        8918 bp DNA     circular SYN 25-JAN-2022
DEFINITION  pCAG-loxPSTOPloxP-Lyn-EGFP.
ACCESSION   pCAG-loxPSTOPloxP-ZsGreen
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 19, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
COMMENT     Backbone is pCAG-loxP-inv[Ta1-Dre-pA]-loxP-Lyn-EGFP 
            (HindIII/BamHIII)Backbone was digested with with 
            PstI/SacImOrange2-rCdk5rap2_1736-1903 was amplified from 
            pCAG-mOrange2-rCdk5rap2_1736-1903 (ST-87) with 
            primers:NewST-PstI_85-103 fw 
            5'-TAGGTCCCTCGACCTGCAGCCTCCGCAGCCCCCAAGCTA-3' NewST-PstI_85-103 rv 
            5'-GTCGAGGCTGATCAGCGAGCTCTCATTACTTATCTAGA-3'PCR product (aprox. 
            1600) was LIC cloned into the digested backbone Product was LIC 
            cloned into the vector.Resistance= 
            AmpicilinVNTEXTCHREPL|Animal/Other Eukaryotic
            VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     source          1..8918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    86..1536
                     /label=CAG promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    86..373
                     /label=CAG_enhancer
     CDS             complement(805..1536)
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MEKGTAARRRTKALPAHFLPAGAAPLRPRPLEGVRRRLPDFGSAR
                     FGTKEVPAPSRTITIKGNGCGSPRLDSRRRSGAAAPLPRALPGPRRPRPARHPHLPPPP
                     ARHGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSPR
                     SPPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPPH
                     KGPSRSPLRLSRSHRKETSRH"
     CDS             complement(846..1349)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPGT
                     ASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAAPP
                     HRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRTKGP
                     PGAL"
     promoter        1686..1704
                     /label=T7_promoter
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     misc_feature    complement(1733..1766)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     polyA_site      complement(1789..2237)
                     /label=rabbit_polyA_terminator
                     /note="/type=polyA_site"
                     /note="/vntifkey=26"
     misc_feature    complement(2247..3329)
                     /label=NLS-HA-Dre
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(3342..4429)
                     /label=Tub-alpha-1 promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    complement(4461..4494)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     misc_feature    4543..5250
                     /label=mOrange2
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    5266..5772
                     /label=insert_rCdk5rap2-1736-1903
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    5822..5827
                     /label=Sac I
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(5837..5854)
                     /label=BGH_rev_primer
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
     terminator      5840..6067
                     /label=bGH_PA_terminator
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
     CDS             6041..6514
                     /codon_start=1
                     /label=ORF frame 2
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSV
                     TATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD"
     promoter        complement(6139..6155)
                     /label=M13_forward20_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    6148..6296
                     /label=lacZ_a
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(6148..6170)
                     /label=M13_pUC_fwd_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     rep_origin      6314..6620
                     /label=f1_origin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        6813..6841
                     /label=AmpR_promoter
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     gene            6883..7743
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             6883..7743
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7898..8517
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        8826..8855
                     /label=lac_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    8869..8891
                     /label=M13_pUC_rev_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        8890..8908
                     /label=M13_reverse_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
      841 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta
     1681 attaataata cgactcacta tagggggtcg acccgccacc aatcatttaa atataacttc
     1741 gtataatgta tgctatacga agttattcgc gatgaataaa tgaaagctgt cgagggatct
     1801 tcataagaga agagggacag ctatgactgg gagtagtcag gagaggagga aaaatctggc
     1861 tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca caaaacaaaa
     1921 tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat ggacagcagg
     1981 gggctgtttc atatactgat gacctcttta tagccacctt tgttcatggc agccagcata
     2041 tggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa tgatttgccc
     2101 tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt gcaatgaaaa
     2161 taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg tccccataat
     2221 ttttggcaga gggaaaaaga tctgactcag ctatccatca gtcgagaatt ggctcccacg
     2281 gacactccgc cccttctgag ataccgcatc agtgtttcag gcttcttcca tgtcccttcc
     2341 tgcataatct cgaccataga cacctgtttc tcggccatgt ctattgcggc acccaccctg
     2401 gcggaatggc cggtccaggt cctatacctc cctttattgg gggtggcgtc ccgtttgttc
     2461 agcagcaccc aggcatcact gaaaatcttc tccatggcag gggcggtcag gggcgtggta
     2521 gtaatgcggg ctttgtttga ccggtgtata ggtgggaaga gcacagcgtc tggatgttct
     2581 ctaagcccac tgacgtccag ccagtcattg agcactgctg tggttcgccg ggagagcact
     2641 ttgtcaaggc ccgcagctgt ggtgattgtc ttagtgtgac tgatgtgcag ggtcactgtg
     2701 tctcctgtct gatccaagtc cccaaccctg atgcgtgata tttcagacat tctcatgagg
     2761 gtgttatagg ctacgaacag gaaagccctg ttgcgcaggt cgaccagccg ctcgctcctg
     2821 ctgagcagca catccagcag cttcaggtcg tcccagcgca gggggattgc ctgtcctgtc
     2881 ctctcaccct tttcggttgc ggcttctctt ctaattcttc tcatggcaag actcactgac
     2941 ttgtcgtcgg acaagggtgg cagtccgcag tgggacagaa gcatattaag catagcatag
     3001 tgcttgtcga tggtagtgga ggccaggtct gcatcgtgca actgaagaaa gtattcgcgt
     3061 gccatttcag gactaattgg aaaccatgca agctgccgag catggcacca tctggcccag
     3121 ctatgaaaga ccaacctcaa atccctcaaa gtgttaggag cgtacgcccc ctggtcattc
     3181 ataaacctca tgaagttttc agcggcctcc tgatactctt tgccgatgtt tcgcaggaat
     3241 ccaccagatg aaccactaat aatcagctca gaggatccag cgtaatctgg aacatcgtat
     3301 gggtaaacct tcctcttctt cttaggcatg gtggcgaatt cgttgctgct tcgcggctgc
     3361 cgggtggatg gcggaggcga ggaggaggtg ttgcttctta cagcgcgact cttaagcggt
     3421 cgatgtaaga gaacctgcgg cacttgggcg gatgcgcctc cttatataca gctcggtcac
     3481 catggggatg ggccaaggcc ttttcctgtt ggtccagacc cctccgtctg cccagagaag
     3541 ccgcgacagg agagtggtga ttggtgcaag cgacatggta tgaggtaatc tctcccccct
     3601 tccccccctc gctagcatcc gtagattttg catccttcag acaaaggcct cgcaaaagca
     3661 actagccgag catccattat ggggtggagc tggggagggg tagagaggga agaaagaaaa
     3721 cgggggttgg gtgattatac ggagcttaaa ccttaaactg tagttaggat aatttaaggt
     3781 tttccccaac acttaatcac agaatttgtg ttgagattct ttatggtaaa ttccagcttt
     3841 tcatttatac tacactaaat ttaaatacgt tccgtcagga ttcaacatct ctgttcgctt
     3901 taaataaagt agcaagagct atgggaatga tggtcaaaaa ggcgtgggta aatgtttagt
     3961 tttacagttg tattattgtg gttattatta ttactattat tattaatatt tgagcaaaat
     4021 tcagaagctc agtatgaata gaccacgcct atgaccctca acagcacccg ggaggcagag
     4081 gcaggaagac ttgcagctca aggtcatcat aaccgccctt agtggtttat gcctgtaaat
     4141 cccagcattg ggaagcggag gactgcggtt attttgtacc cggcctggat atatagtgtg
     4201 atatcctgtc tcaaaaacca aaaacaaaca aagagagaga gagagagaaa ggaaaaaaga
     4261 gaaacaatat acaaagtagt acttaacgct ttactagaga gcttgctttc tttagcggta
     4321 gtgctctccc atcacccctc cccgctcgcc cccttacccg accctacctc accccagagc
     4381 ctgtgttatt ttgtccctag aaatgagcca tccctcctaa tacggaatga tccccatcaa
     4441 gctgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc
     4501 cctcgacctg cagcctccgc agcccccaag ctagcagcca ccatggtgag caagggcgag
     4561 gagaataaca tggccatcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc
     4621 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcttt
     4681 cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     4741 tcccctcatt tcacctacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     4801 tacttcaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa ctacgaggac
     4861 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     4921 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg
     4981 ggctgggagg cctcctccga gcggatgtac cccgaggacg gtgccctgaa gggcaagatc
     5041 aagatgaggc tgaagctgaa ggacggcggc cactacacct ccgaggtcaa gaccacctac
     5101 aaggccaaga agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc
     5161 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     5221 tccaccggcg gcatggacga gctgtacaag tcaggactca gatctcacat gctgtgcctg
     5281 attgaggact atgatgccct ctataagcag atcagctggg gccagacact gcttgccaag
     5341 atggatattc aaacccaaga ggctctgagc cccacaagtc agaagctggg accaaaggct
     5401 tcattctctg tgcctctgag caagtttctc tccagcatga acacagccaa gctgatcctg
     5461 gaaaaagcct ccaggttgct gaagctcttc tggagggtct ctgtccccac caacggccag
     5521 tgttcccttc actgtgacca gattggagaa atgaaggcag agatcaccaa actacacaaa
     5581 aaattgtttg aacaagaaaa gaagctgcag aacacagcaa aacttctgca gcagagcaag
     5641 caccaggaga aaatcatctt tgatcagttg gtcatcaccc accaagtcct tcggaaagcc
     5701 aggggaaacc tggagctcag gcctagagct gcccatccag gaacatccag tcccagcaga
     5761 ccgggctcat gagtcgacgg taccgcgggc ccgggatcca ccggatctag ataagtaatg
     5821 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5881 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5941 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6001 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6061 tctatggctt ctgaggcgga aagaaccagc tggggctcga gatccactag ttctagcctc
     6121 gaggctagag cggccgccac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     6181 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     6241 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     6301 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     6361 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6421 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     6481 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     6541 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     6601 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6661 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6721 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     6781 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6841 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6901 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6961 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7021 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7081 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     7141 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7201 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7261 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7321 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7381 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     7441 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     7501 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     7561 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     7621 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     7681 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7741 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7801 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7861 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7921 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7981 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8041 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     8101 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8161 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8221 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8281 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8341 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8401 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8461 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8521 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8581 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8641 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8701 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     8761 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     8821 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     8881 tttcacacag gaaacagcta tgaccatgag gcgcgccg
//
