LOCUS       pCAG-lox-inv[Ta1        8849 bp DNA     circular SYN 24-JAN-2022
DEFINITION  pCAG-loxPSTOPloxP-Lyn-EGFP.
ACCESSION   pCAG-loxPSTOPloxP-ZsGreen
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     Backbone is 
            pCAG-lox[invTa1-iCre-pA]loxP-Lyn-EGFPpCAG-lox[invTa1-iCre-pA]loxP-Ly
            n-EGFP was diggested with PstI/SacI releasing 
            lyn-GFPmOrange2-rCdk5rap2_1736-1903 was amplified from 
            pCAG-mOrange2-rCdk5rap2_1736-1903 (ST-87) with 
            primers:NewST-PstI_85-103 fw 
            5'-TAGGTCCCTCGACCTGCAGCCTCCGCAGCCCCCAAGCTA-3' NewST-PstI_85-103 rv 
            5'-GTCGAGGCTGATCAGCGAGCTCTCATTACTTATCTAGA-3'PCR product (aprox. 
            1600) was LIC cloned into the digested backbone Product was LIC 
            cloned into the vector.Resistance= 
            AmpicilinVNTEXTCHREPL|Animal/Other Eukaryotic
            VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     source          1..8849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    86..1536
                     /label=CAG promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    86..373
                     /label=CAG_enhancer
     CDS             complement(805..1536)
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MEKGTAARRRTKALPAHFLPAGAAPLRPRPLEGVRRRLPDFGSAR
                     FGTKEVPAPSRTITIKGNGCGSPRLDSRRRSGAAAPLPRALPGPRRPRPARHPHLPPPP
                     ARHGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSPR
                     SPPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPPH
                     KGPSRSPLRLSRSHRKETSRH"
     CDS             complement(846..1349)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPGT
                     ASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAAPP
                     HRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRTKGP
                     PGAL"
     promoter        1686..1704
                     /label=T7_promoter
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     misc_feature    complement(1733..1766)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     polyA_site      complement(1784..2214)
                     /label=rabbit_polyA_terminator
                     /note="/type=polyA_site"
                     /note="/vntifkey=26"
     misc_feature    complement(2221..3276)
                     /label=NLS-iCre
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(3289..4376)
                     /label=Tub-alpha-1
                     /label=nonstandard type: eukaryotic
                     /note="Tubulin-alpha-1 promoter"
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    complement(4392..4425)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     misc_feature    4439..4444
                     /label=Pst I
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    4474..5181
                     /label=mOrange2
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    5197..5703
                     /label=insert_rCdk5rap2-1736-1903
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    5753..5758
                     /label=Sac I
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(5768..5785)
                     /label=BGH_rev_primer
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
     terminator      5771..5998
                     /label=bGH_PA_terminator
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
     CDS             5972..6445
                     /codon_start=1
                     /label=ORF frame 2
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSV
                     TATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD"
     promoter        complement(6070..6086)
                     /label=M13_forward20_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    6079..6227
                     /label=lacZ_a
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(6079..6101)
                     /label=M13_pUC_fwd_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     rep_origin      6245..6551
                     /label=f1_origin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        6744..6772
                     /label=AmpR_promoter
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     gene            6814..7674
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             6814..7674
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7829..8448
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        8757..8786
                     /label=lac_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    8800..8822
                     /label=M13_pUC_rev_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        8821..8839
                     /label=M13_reverse_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
      841 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta
     1681 attaataata cgactcacta tagggggtcg acccgccacc aatcatttaa atataacttc
     1741 gtataatgta tgctatacga agttattcgc gatgaataaa tgagagaaga gggacagcta
     1801 tgactgggag tagtcaggag aggaggaaaa atctggctag taaaacatgt aaggaaaatt
     1861 ttagggatgt taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc taacctcaag
     1921 tcaaggcttt tctatggaat aaggaatgga cagcaggggg ctgtttcata tactgatgac
     1981 ctctttatag ccacctttgt tcatggcagc cagcatatgg catatgttgc caaactctaa
     2041 accaaatact cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag
     2101 agacacaaaa aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa
     2161 gtcagatgct caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct
     2221 tcagtcccca tcctcgagca gcctcaccat ggccccagtc tcagagtcca ggtttctgat
     2281 gtagttcatc acaatgttca cattggtcca gccaccagcc tgcatgattt cagggatgga
     2341 cacaccagcc ctggccatgt ccctggcagc acccactctg gcagagtggc cagaccaggc
     2401 caggtatctc tgcccagagt catccttggc accatagatc aggcggtggg tggcctcaaa
     2461 gatcccttcc agggcccggg tggacagttg ggaggtggca gaaggggcag ccacaccatt
     2521 ctttctgacc cggcagaaca ggtagttgtt ggggtcatca gccacaccag acacagagat
     2581 ccatctctcc accagcttgg taacccccag ggacagggcc ttctccacac cagctgtgga
     2641 caccagggtc ttggtcctgc caatgtggat cagcattctc ccaccatcgg tgcgggagat
     2701 gtccttcact ctgattctgg caatttcggc aatgcgcagc agggtgttgt aggcaatgcc
     2761 caggaaggcc aggttcctga tgtcctggca tctgtcagag ttctccatca gggatctgac
     2821 ttggtcaaag tcagtgcgtt caaaggccag ggcctgcttg gctctctccc cagcatccac
     2881 attctccttt ctgattctcc tcatcaccag ggacacagca ttggagtcag aagggcgagg
     2941 caggccagat ctcctgtgca gcatgttgag ctggcccagg tgctgttgga tggtcttcac
     3001 agccaggcct ctggcttgca ggtacaggag gtagtccctc acatcctcag gttcagcagg
     3061 gaaccatttc ctgttgttca gcttgcacca ggcagcccag gatctgcaca cagacaggag
     3121 catcttccag gtgtgttcag agaaggcctg cctgtccctg aacatgtcca tcaggttctt
     3181 cctgacttca tcagaggtgg catccacagg gagggcaggc aggttttggt gcacagtcag
     3241 caggttggag actttcctct tcttcttggg caccatggtg gcgaattcgt tgctgcttcg
     3301 cggctgccgg gtggatggcg gaggcgagga ggaggtgttg cttcttacag cgcgactctt
     3361 aagcggtcga tgtaagagaa cctgcggcac ttgggcggat gcgcctcctt atatacagct
     3421 cggtcaccat ggggatgggc caaggccttt tcctgttggt ccagacccct ccgtctgccc
     3481 agagaagccg cgacaggaga gtggtgattg gtgcaagcga catggtatga ggtaatctct
     3541 ccccccttcc ccccctcgct agcatccgta gattttgcat ccttcagaca aaggcctcgc
     3601 aaaagcaact agccgagcat ccattatggg gtggagctgg ggaggggtag agagggaaga
     3661 aagaaaacgg gggttgggtg attatacgga gcttaaacct taaactgtag ttaggataat
     3721 ttaaggtttt ccccaacact taatcacaga atttgtgttg agattcttta tggtaaattc
     3781 cagcttttca tttatactac actaaattta aatacgttcc gtcaggattc aacatctctg
     3841 ttcgctttaa ataaagtagc aagagctatg ggaatgatgg tcaaaaaggc gtgggtaaat
     3901 gtttagtttt acagttgtat tattgtggtt attattatta ctattattat taatatttga
     3961 gcaaaattca gaagctcagt atgaatagac cacgcctatg accctcaaca gcacccggga
     4021 ggcagaggca ggaagacttg cagctcaagg tcatcataac cgcccttagt ggtttatgcc
     4081 tgtaaatccc agcattggga agcggaggac tgcggttatt ttgtacccgg cctggatata
     4141 tagtgtgata tcctgtctca aaaaccaaaa acaaacaaag agagagagag agagaaagga
     4201 aaaaagagaa acaatataca aagtagtact taacgcttta ctagagagct tgctttcttt
     4261 agcggtagtg ctctcccatc acccctcccc gctcgccccc ttacccgacc ctacctcacc
     4321 ccagagcctg tgttattttg tccctagaaa tgagccatcc ctcctaatac ggaatttccg
     4381 gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt ccctcgacct
     4441 gcagcctccg cagcccccaa gctagcagcc accatggtga gcaagggcga ggagaataac
     4501 atggccatca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc
     4561 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggctt tcagaccgct
     4621 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcat
     4681 ttcacctacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttcaag
     4741 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga actacgagga cggcggcgtg
     4801 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     4861 cgcggcacca acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag
     4921 gcctcctccg agcggatgta ccccgaggac ggtgccctga agggcaagat caagatgagg
     4981 ctgaagctga aggacggcgg ccactacacc tccgaggtca agaccaccta caaggccaag
     5041 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cacctcccac
     5101 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     5161 ggcatggacg agctgtacaa gtcaggactc agatctcaca tgctgtgcct gattgaggac
     5221 tatgatgccc tctataagca gatcagctgg ggccagacac tgcttgccaa gatggatatt
     5281 caaacccaag aggctctgag ccccacaagt cagaagctgg gaccaaaggc ttcattctct
     5341 gtgcctctga gcaagtttct ctccagcatg aacacagcca agctgatcct ggaaaaagcc
     5401 tccaggttgc tgaagctctt ctggagggtc tctgtcccca ccaacggcca gtgttccctt
     5461 cactgtgacc agattggaga aatgaaggca gagatcacca aactacacaa aaaattgttt
     5521 gaacaagaaa agaagctgca gaacacagca aaacttctgc agcagagcaa gcaccaggag
     5581 aaaatcatct ttgatcagtt ggtcatcacc caccaagtcc ttcggaaagc caggggaaac
     5641 ctggagctca ggcctagagc tgcccatcca ggaacatcca gtcccagcag accgggctca
     5701 tgagtcgacg gtaccgcggg cccgggatcc accggatcta gataagtaat gagagctcgc
     5761 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5821 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     5881 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5941 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     6001 tctgaggcgg aaagaaccag ctggggctcg agatccacta gttctagcct cgaggctaga
     6061 gcggccgcca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6121 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6181 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6241 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6301 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6361 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6421 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6481 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6541 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6601 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6661 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     6721 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6781 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6841 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6901 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6961 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7021 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7081 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7141 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7201 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7261 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7321 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7381 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7441 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7501 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7561 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7621 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7681 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7741 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7801 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7861 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7921 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7981 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8041 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8101 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8161 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8221 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8281 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8341 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8401 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8461 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8521 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8581 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8641 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     8701 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8761 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8821 ggaaacagct atgaccatga ggcgcgccg
//
