LOCUS       pCAG-DIO-inv[rox        7542 bp DNA     circular SYN 17-JUN-2022
DEFINITION  pCAG-loxPSTOPloxP-Lyn-EGFP.
ACCESSION   pCAG-loxPSTOPloxP-ZsGreen
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     Backbone is pCAG-DIO-inv[RSR-LynGFP-bGH]
            Backbone was digested with PstI/SacI to release Lyn-EGFPEB3-Neon was
            amplified from pCMV-EB3-NeonGreen with primers:
            CMV fw 5'-CGCAAATGGGCGGTAGGCGTG-3' (Sequencing primer) 
            mGFP,NeonGFP-SacI-Rv 
            5'-CAGTCGAGGCTGATCAGCGAGCTCTTACTTGTACAGCTCGTCCAT-3' (#16)The PCR 
            poduct was Diggested with PstI and subsequently cloned in an hybrid 
            fashion in a single reaction by gibson assembly/regular 
            cloningResistance= Ampicilin
            VNTEXTCHREPL|Animal/Other Eukaryotic
            VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     source          1..7542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    86..1539
                     /label=CAG promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    86..373
                     /label=CAG_enhancer
     CDS             complement(805..1539)
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MEKGTAARRRTKALPAHFLPAGAAPLRPAPARGGAAAPPRFRLRT
                     DLGQRKSLRPLARLP*KAMAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPP
                     PARHGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSP
                     RSPPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPP
                     HKGPSRSPLRLSRSHRKETSRH"
     CDS             complement(846..1350)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPARH
                     GEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSPRSPP
                     APRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPPHKGP
                     SRSPL"
     misc_recomb     1657..1690
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
                     /note="/bound_moiety=Cre recombinase"
                     /note="/type=misc_recomb"
                     /note="/vntifkey=86"
     misc_recomb     1741..1774
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="/bound_moiety=Cre recombinase"
                     /note="/type=misc_recomb"
                     /note="/vntifkey=86"
     misc_feature    1805..1810
                     /label=NheI
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     polyA_signal    complement(1833..2057)
                     /label=bGH polyA signal
                     /note="/type=polyA_signal"
                     /note="/vntifkey=25"
     misc_feature    complement(2079..3647)
                     /label=EB3-mNeon
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(2079..2789)
                     /label=mNeon
                     /label=nonstandard type: misc_marker
                     /note="/type=misc_marker"
                     /note="/vntifkey=22"
     misc_feature    complement(2805..3647)
                     /label=EB3
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_recomb     complement(3676..3707)
                     /label=Rox recombination site
                     /note="/type=misc_recomb"
                     /note="/vntifkey=86"
     misc_structure  complement(3736..4375)
                     /label=3 x sv40 STOP cassette
                     /note="/type=misc_structure"
                     /note="/vntifkey=88"
     polyA_signal    complement(3736..3867)
                     /label=sv40 polyA signal
                     /note="/type=polyA_signal"
                     /note="/vntifkey=25"
     polyA_signal    complement(3990..4121)
                     /label=sv40 polyA signal
                     /note="/type=polyA_signal"
                     /note="/vntifkey=25"
     polyA_signal    complement(4244..4375)
                     /label=sv40 polyA signal
                     /note="/type=polyA_signal"
                     /note="/vntifkey=25"
     misc_recomb     complement(4545..4576)
                     /label=Rox recombination site
                     /note="/type=misc_recomb"
                     /note="/vntifkey=86"
     misc_feature    4596..4601
                     /label=MluI
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_recomb     complement(4622..4655)
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
                     /note="/bound_moiety=Cre recombinase"
                     /note="/type=misc_recomb"
                     /note="/vntifkey=86"
     misc_recomb     complement(4706..4739)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="/bound_moiety=Cre recombinase"
                     /note="/type=misc_recomb"
                     /note="/vntifkey=86"
     CDS             4756..5138
                     /codon_start=1
                     /label=ORF frame 2
                     /translation="MRHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALP
                     NSCAA*MANGTRPVAAH*ARRVWWLRAA*PLHLPAP*RPLLSLSSLPFSPRSPAFPVKL
                     *IGGSL*GSDLVLYGTSTPKNLI"
     promoter        complement(4763..4779)
                     /label=M13_forward20_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    4772..4920
                     /label=lacZ_a
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(4772..4794)
                     /label=M13_pUC_fwd_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     rep_origin      4938..5244
                     /label=f1_origin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        5437..5465
                     /label=AmpR_promoter
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     gene            5507..6367
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             5507..6367
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6522..7141
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        7450..7479
                     /label=lac_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    7493..7515
                     /label=M13_pUC_rev_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        7514..7532
                     /label=M13_reverse_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
      841 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagaccagg cagactcgac tccggaataa cttcgtatag gatactttat
     1681 acgaagttat gcagaatggt agctggattg tagctgctat tagcaatatg aaacctctta
     1741 ataacttcgt atagcataca ttatacgaag ttatggcgcg ttacttgtac agctagagca
     1801 actagctagc gaggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg
     1861 cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat
     1921 agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt
     1981 gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg
     2041 gcaactagaa ggcacagtcg aggctgatca gcgagctctt acttgtacag ctcgtccatg
     2101 cccatcacat cggtaaaggc cttttgccac tccttgaagt tgagctcggt cttggagtgc
     2161 ttgagctccg tcttacggaa cacgtacatc ggctggttct tcagatagtt agccgccatt
     2221 ggcttggcaa aggtgtaggt ggtccgcgca gtgctccggt agcgcttgcc atttccagtg
     2281 gtgtaactcc acttaaaggt actgatgatg gttttgtcgt tggggtaagt cttcttcgac
     2341 ctgcaccagt ccgcagcggt cagcgagttg gtcatcacag gaccgtcagc agggaaacca
     2401 gtccccttca cctgggcctc tcctttgatg tggcttccct cgtaggtgta gcggtagtta
     2461 acagtaaggg aggcaccatc ttcaaactgc attgtgcgat ggacttggta gccggagcca
     2521 tctaccatgg cggcctggaa aggcgacatc ccgtcagggt agggcaggta ctgatggaag
     2581 ccatacccga tatgagggac cagaatccag ggggagaact ggaggtcacc cttggtggac
     2641 ttcaggttta actcctcata accatcattt ggattgccgg tgccctgacc caccatgtca
     2701 aagtccacac cgttgatgga gccaaagatg tgtaactcat gtgtcgctgg gagagaggcc
     2761 atgttatcct cctcgccctt gctcaccatg gtggcgggat ccaggtactc gtcctggtct
     2821 tcttgttgat gctcttcaat ctcatcgtcc tcagggggtg cgaatccttc ctctgtggca
     2881 tagaggatgc caatgatgcc tgagataaca gggctgtttt cactttcatg ctcctggcag
     2941 atgagctcga tgtcacgaag tttgctgaag tagaagtcac gttccttctc cagcccatcc
     3001 actgtcagct tcaagtccac cagctgttgg ttgagttcaa gaatttgggc atcagtctca
     3061 tggccgccat ttcgggctga tggaggattc ttccggagaa tgcagggggg ggccacattg
     3121 ctcagccggc cagaggtctg catgtttttt gggcctgtgg gggacgtcct ctgtggaact
     3181 gctgtgccaa tgagtttctt ggatttgttg aagatctgat cacctgggtt aggaggtggc
     3241 gctacgtcct ggccctgccg cgccagcaga gggttgtaat cctttccatc atagtttgcg
     3301 tcaaagaatt tcttaaacca ctgaataaac tcaaaattat cttggaattt tcctttcact
     3361 aatttctcta caggaatgat tttgtcaaca cccatcttct tgaaagctgc ttgcagcacc
     3421 ttgaagttgt ggatgtattc atgctctagt ttggcctgga acttcacttt cctcaagtgc
     3481 acacagccgg ggaagagcat gtccatgaac tggcagtagg ctgcccctga acaaagctgt
     3541 tctatcttgg tatagttgag gtgcagggag tcgttgaccc atgcaagcat atcatggcga
     3601 ctcagatttt cactggtcac agatgtggag tacacattga cggccatggt ggcagtcgac
     3661 tgcagggcgg ctgcctaact ttaaataatg ccaattattt aaagttaatt aagggttccg
     3721 gatcagcttg atggggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     3781 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     3841 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     3901 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     3961 atggctgatt atgatcctct agagtcgcag atccagacat gataagatac attgatgagt
     4021 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     4081 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     4141 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     4201 tctacaaatg tggtatggct gattatgatc ctctagagtc gcagatccag acatgataag
     4261 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     4321 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     4381 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     4441 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag agtcgcagat
     4501 cctctagagt cgcagatctg caagctttca tttattcatc gcgataactt taaataatgc
     4561 caattattta aagttaggtg gcgggtcgac cccctacgcg tcacgcctat ggtggcctag
     4621 cataacttcg tataaagtat cctatacgaa gttatttgcc ttaacccaga aattatcact
     4681 gttattcttt agaatggtgc aaagaataac ttcgtataat gtatgctata cgaagttatg
     4741 aattcgatat caagcggccg ccactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4801 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4861 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     4921 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4981 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5041 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5101 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5161 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5221 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5281 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5341 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     5401 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5461 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5521 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5581 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5641 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5701 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5761 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5821 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5881 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5941 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6001 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6061 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6121 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6181 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6241 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6301 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6361 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6421 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6481 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6661 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6721 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6781 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6841 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6901 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6961 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7021 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7081 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7141 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7201 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7261 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7321 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7381 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     7441 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     7501 acaatttcac acaggaaaca gctatgacca tgaggcgcgc cg
//
