LOCUS       pCAG-lox-inv[Ta1        8835 bp DNA     circular SYN 07-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   DNA_pCAG-lox-inv[Ta1-iCre-pA]-lox-mCherry-hCentrin1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     Backbone is pCAG-lox[invTa1-iCre-pA]loxP-Lyn-EGFP
            pCAG-lox[invTa1-iCre-pA]loxP-Lyn-EGFP was diggested with PstI/SacI 
            releasing lyn-GFP
            PCR1: mNeonGreen was amplified from pCMV-mNeonGreen-C1 with primers:
            
            ST-PstI_mScarlet_orange fw 
            5'-TAGGTCCCTCGACCTGCAGGCCACCATGGTGAGCAAGGG-3'
            mScarlet_orange-linker rv 
            5'-TTGAGCTCGAGATCTGAGTCCGGACTTGTACAGCTCGTCCAT-3'
            PCR2: linker-hCentrin was amplified from 
            pCAG-lox-inv[Ta1-iCre-pA]-lox-mCherry-hCentrin with primers:
            Linker-hCentrin2 fw
            5'-TCCGGACTCAGATCTCGAGCTCAA-3'
            New_ST-SacI_hCentrin rv 
            5'-GTCGAGGCTGATCAGCGAGCTCTCAGTAAAGGCTGGTCTTC-3'(# 613).
            PCR1 and PCR2 were LIC cloned into the above digested backbone.
            Resistance= Ampicilin
FEATURES             Location/Qualifiers
     source          1..8835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    86..1536
                     /label=CAG promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    86..373
                     /label=CAG_enhancer
                     /note="/vntifkey=21"
     CDS             complement(805..1536)
                     /codon_start=1
                     /label=ORF frame 3
                     /note="/vntifkey=4"
                     /translation="MEKGTAARRRTKALPAHFLPAGAAPLRPRPLEGVRRRLPDFGSAR
                     FGTKEVPAPSRTITIKGNGCGSPRLDSRRRSGAAAPLPRALPGPRRPRPARHPHLPPPP
                     ARHGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSPR
                     SPPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPPH
                     KGPSRSPLRLSRSHRKETSRH"
     CDS             complement(846..1349)
                     /codon_start=1
                     /label=ORF frame 1
                     /note="/vntifkey=4"
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPGT
                     ASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAAPP
                     HRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRTKGP
                     PGAL"
     promoter        1686..1704
                     /label=T7_promoter
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
                     /note="/vntifkey=30"
     misc_feature    complement(1733..1766)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
                     /note="/vntifkey=21"
     polyA_site      complement(1784..2214)
                     /label=rabbit_polyA_terminator
                     /note="/type=polyA_site"
                     /note="/vntifkey=26"
     misc_feature    complement(2221..3276)
                     /label=NLS-iCre
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(3289..4376)
                     /label=Tub-alpha-1
                     /label=nonstandard type: eukaryotic
                     /note="Tubulin-alpha-1 promoter"
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    complement(4392..4425)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
                     /note="/vntifkey=21"
     misc_feature    4439..4444
                     /label=Pst I
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    4461..5738
                     /label=mNeonGreen-linker-hCentrin1
                     /label=mScarlet-linker-hCentrin1
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    4461..5168
                     /label=NeonGreen GFP
                     /label=nonstandard type: misc_marker
                     /note="/type=misc_marker"
                     /note="/vntifkey=22"
     misc_feature    5169..5219
                     /label=Linker
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    5220..5738
                     /label=human Centrin1
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    5739..5744
                     /label=Sac I
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(5754..5771)
                     /label=BGH_rev_primer
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
                     /note="/vntifkey=21"
     terminator      5757..5984
                     /label=bGH_PA_terminator
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
                     /note="/vntifkey=43"
     CDS             5958..6431
                     /codon_start=1
                     /label=ORF frame 2
                     /note="/vntifkey=4"
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSV
                     TATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD"
     promoter        complement(6056..6072)
                     /label=M13_forward20_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=30"
     misc_feature    6065..6213
                     /label=lacZ_a
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=21"
     misc_feature    complement(6065..6087)
                     /label=M13_pUC_fwd_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=21"
     rep_origin      6231..6537
                     /label=f1_origin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=33"
     promoter        6730..6758
                     /label=AmpR_promoter
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=30"
     gene            6800..7660
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=60"
     CDS             6800..7660
                     /codon_start=1
                     /label=ORF frame 1
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7815..8434
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="/vntifkey=33"
     promoter        8743..8772
                     /label=lac_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="/vntifkey=30"
     misc_feature    8786..8808
                     /label=M13_pUC_rev_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="/vntifkey=21"
     promoter        8807..8825
                     /label=M13_reverse_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="/vntifkey=30"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
      841 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta
     1681 attaataata cgactcacta tagggggtcg acccgccacc aatcatttaa atataacttc
     1741 gtataatgta tgctatacga agttattcgc gatgaataaa tgagagaaga gggacagcta
     1801 tgactgggag tagtcaggag aggaggaaaa atctggctag taaaacatgt aaggaaaatt
     1861 ttagggatgt taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc taacctcaag
     1921 tcaaggcttt tctatggaat aaggaatgga cagcaggggg ctgtttcata tactgatgac
     1981 ctctttatag ccacctttgt tcatggcagc cagcatatgg catatgttgc caaactctaa
     2041 accaaatact cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag
     2101 agacacaaaa aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa
     2161 gtcagatgct caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct
     2221 tcagtcccca tcctcgagca gcctcaccat ggccccagtc tcagagtcca ggtttctgat
     2281 gtagttcatc acaatgttca cattggtcca gccaccagcc tgcatgattt cagggatgga
     2341 cacaccagcc ctggccatgt ccctggcagc acccactctg gcagagtggc cagaccaggc
     2401 caggtatctc tgcccagagt catccttggc accatagatc aggcggtggg tggcctcaaa
     2461 gatcccttcc agggcccggg tggacagttg ggaggtggca gaaggggcag ccacaccatt
     2521 ctttctgacc cggcagaaca ggtagttgtt ggggtcatca gccacaccag acacagagat
     2581 ccatctctcc accagcttgg taacccccag ggacagggcc ttctccacac cagctgtgga
     2641 caccagggtc ttggtcctgc caatgtggat cagcattctc ccaccatcgg tgcgggagat
     2701 gtccttcact ctgattctgg caatttcggc aatgcgcagc agggtgttgt aggcaatgcc
     2761 caggaaggcc aggttcctga tgtcctggca tctgtcagag ttctccatca gggatctgac
     2821 ttggtcaaag tcagtgcgtt caaaggccag ggcctgcttg gctctctccc cagcatccac
     2881 attctccttt ctgattctcc tcatcaccag ggacacagca ttggagtcag aagggcgagg
     2941 caggccagat ctcctgtgca gcatgttgag ctggcccagg tgctgttgga tggtcttcac
     3001 agccaggcct ctggcttgca ggtacaggag gtagtccctc acatcctcag gttcagcagg
     3061 gaaccatttc ctgttgttca gcttgcacca ggcagcccag gatctgcaca cagacaggag
     3121 catcttccag gtgtgttcag agaaggcctg cctgtccctg aacatgtcca tcaggttctt
     3181 cctgacttca tcagaggtgg catccacagg gagggcaggc aggttttggt gcacagtcag
     3241 caggttggag actttcctct tcttcttggg caccatggtg gcgaattcgt tgctgcttcg
     3301 cggctgccgg gtggatggcg gaggcgagga ggaggtgttg cttcttacag cgcgactctt
     3361 aagcggtcga tgtaagagaa cctgcggcac ttgggcggat gcgcctcctt atatacagct
     3421 cggtcaccat ggggatgggc caaggccttt tcctgttggt ccagacccct ccgtctgccc
     3481 agagaagccg cgacaggaga gtggtgattg gtgcaagcga catggtatga ggtaatctct
     3541 ccccccttcc ccccctcgct agcatccgta gattttgcat ccttcagaca aaggcctcgc
     3601 aaaagcaact agccgagcat ccattatggg gtggagctgg ggaggggtag agagggaaga
     3661 aagaaaacgg gggttgggtg attatacgga gcttaaacct taaactgtag ttaggataat
     3721 ttaaggtttt ccccaacact taatcacaga atttgtgttg agattcttta tggtaaattc
     3781 cagcttttca tttatactac actaaattta aatacgttcc gtcaggattc aacatctctg
     3841 ttcgctttaa ataaagtagc aagagctatg ggaatgatgg tcaaaaaggc gtgggtaaat
     3901 gtttagtttt acagttgtat tattgtggtt attattatta ctattattat taatatttga
     3961 gcaaaattca gaagctcagt atgaatagac cacgcctatg accctcaaca gcacccggga
     4021 ggcagaggca ggaagacttg cagctcaagg tcatcataac cgcccttagt ggtttatgcc
     4081 tgtaaatccc agcattggga agcggaggac tgcggttatt ttgtacccgg cctggatata
     4141 tagtgtgata tcctgtctca aaaaccaaaa acaaacaaag agagagagag agagaaagga
     4201 aaaaagagaa acaatataca aagtagtact taacgcttta ctagagagct tgctttcttt
     4261 agcggtagtg ctctcccatc acccctcccc gctcgccccc ttacccgacc ctacctcacc
     4321 ccagagcctg tgttattttg tccctagaaa tgagccatcc ctcctaatac ggaatttccg
     4381 gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt ccctcgacct
     4441 gcaggctacc ggtcgccacc atggtgagca agggcgagga ggataacatg gcctctctcc
     4501 cagcgacaca tgagttacac atctttggct ccatcaacgg tgtggacttt gacatggtgg
     4561 gtcagggcac cggcaatcca aatgatggtt atgaggagtt aaacctgaag tccaccaagg
     4621 gtgacctcca gttctccccc tggattctgg tccctcatat cgggtatggc ttccatcagt
     4681 acctgcccta ccctgacggg atgtcgcctt tccaggccgc catggtagat ggctccggct
     4741 accaagtcca tcgcacaatg cagtttgaag atggtgcctc ccttactgtt aactaccgct
     4801 acacctacga gggaagccac atcaaaggag aggcccaggt gaaggggact ggtttccctg
     4861 ctgacggtcc tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg tcgaagaaga
     4921 cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc actggaaatg
     4981 gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca atggcggcta
     5041 actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag cactccaaga
     5101 ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc atggacgagc
     5161 tgtacaagtc cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtacca
     5221 tggcttccgg cttcaagaag cccagcgctg cctccaccgg ccaaaagaga aaggtggcac
     5281 ctaagcccga gctcactgag gatcagaagc aagaagttcg ggaagcattt gacctcttcg
     5341 acgtggacgg aagtgggacc atcgacgcga aggagctgaa ggtggccatg agagcgctgg
     5401 gcttcgaacc caggaaggaa gagatgaaga aaatgatctc cgaggtggac agggaaggca
     5461 cggggaagat cagcttcaat gacttcctgg ccgtgatgac gcagaagatg tccgagaagg
     5521 acaccaaaga agaaatcctg aaggccttca ggctctttga tgacgatgag accgggaaga
     5581 tctcgttcaa aaacctgaag cgtgtggcca acgagctggg ggagaacctc acggatgagg
     5641 agctgcagga gatgatcgac gaagctgatc gggatgggga cggcgaagtg aacgaggagg
     5701 agttccttcg gatcatgaag aagaccagcc tttactgaga gctcgctgat cagcctcgac
     5761 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5821 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5881 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5941 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     6001 aaccagctgg ggctcgagat ccactagttc tagcctcgag gctagagcgg ccgccactgg
     6061 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     6121 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     6181 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     6241 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6301 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6361 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6421 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6481 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6541 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6601 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     6661 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6721 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6781 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6841 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6901 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6961 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7021 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     7081 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     7141 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     7201 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     7261 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     7321 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     7381 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     7441 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     7501 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     7561 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7621 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7681 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7741 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7801 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7861 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7921 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     7981 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8041 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8161 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8221 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8281 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8341 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8401 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8461 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8521 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8581 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     8641 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     8701 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8761 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     8821 ccatgaggcg cgccg
//
