LOCUS       pCAG-lox-inv[Ta1       12710 bp DNA     circular SYN 30-MAY-2022
DEFINITION  pCAG-loxPSTOPloxP-Lyn-EGFP.
ACCESSION   pCAG-loxPSTOPloxP-ZsGreen
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     Backbone is pCAG-loxP-inv[Ta1-Dre-pA]-loxP-Lyn-EGFP 
            [HindIII/BamHIII]Backbone was digested with with 
            PstI/SacImAkna-Kozak-linker-mOrange2 was amplified 
            frompCAG-mAkna-Kozak-linker-mOrange2 (ST-99) with 
            primers:NewST-PstI_85-103 fw 
            5'-TAGGTCCCTCGACCTGCAGCCTCCGCAGCCCCCAAGCTA-3' NewST-PstI_85-103 rv 
            5'-GTCGAGGCTGATCAGCGAGCTCTCATTACTTATCTAGA-3'PCR product (aprox. 
            5300) was LIC cloned into the digested backbone Product was LIC 
            cloned into the vector.Resistance= 
            AmpicilinVNTEXTCHREPL|Animal/Other Eukaryotic
            VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     source          1..12710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    86..1536
                     /label=CAG promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    86..373
                     /label=CAG_enhancer
     CDS             complement(805..1536)
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MEKGTAARRRTKALPAHFLPAGAAPLRPRPLEGVRRRLPDFGSAR
                     FGTKEVPAPSRTITIKGNGCGSPRLDSRRRSGAAAPLPRALPGPRRPRPARHPHLPPPP
                     ARHGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSPR
                     SPPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPPH
                     KGPSRSPLRLSRSHRKETSRH"
     CDS             complement(846..1349)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPGT
                     ASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAAPP
                     HRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRTKGP
                     PGAL"
     promoter        1686..1704
                     /label=T7_promoter
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     misc_feature    complement(1733..1766)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     polyA_site      complement(1789..2237)
                     /label=rabbit_polyA_terminator
                     /note="/type=polyA_site"
                     /note="/vntifkey=26"
     misc_feature    complement(2247..3329)
                     /label=NLS-HA-Dre
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(3342..4429)
                     /label=Tub-alpha-1 promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    complement(4461..4494)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     misc_feature    4543..8754
                     /label=mAkna
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    8755..8811
                     /label=linker
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    8812..9519
                     /label=mOrange2
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    9614..9619
                     /label=Sac I
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(9629..9646)
                     /label=BGH_rev_primer
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
     terminator      9632..9859
                     /label=bGH_PA_terminator
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
     CDS             9833..10306
                     /codon_start=1
                     /label=ORF frame 2
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSV
                     TATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD"
     promoter        complement(9931..9947)
                     /label=M13_forward20_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    9940..10088
                     /label=lacZ_a
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(9940..9962)
                     /label=M13_pUC_fwd_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     rep_origin      10106..10412
                     /label=f1_origin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        10605..10633
                     /label=AmpR_promoter
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     gene            10675..11535
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             10675..11535
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11690..12309
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        12618..12647
                     /label=lac_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    12661..12683
                     /label=M13_pUC_rev_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        12682..12700
                     /label=M13_reverse_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
      841 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta
     1681 attaataata cgactcacta tagggggtcg acccgccacc aatcatttaa atataacttc
     1741 gtataatgta tgctatacga agttattcgc gatgaataaa tgaaagctgt cgagggatct
     1801 tcataagaga agagggacag ctatgactgg gagtagtcag gagaggagga aaaatctggc
     1861 tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca caaaacaaaa
     1921 tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat ggacagcagg
     1981 gggctgtttc atatactgat gacctcttta tagccacctt tgttcatggc agccagcata
     2041 tggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa tgatttgccc
     2101 tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt gcaatgaaaa
     2161 taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg tccccataat
     2221 ttttggcaga gggaaaaaga tctgactcag ctatccatca gtcgagaatt ggctcccacg
     2281 gacactccgc cccttctgag ataccgcatc agtgtttcag gcttcttcca tgtcccttcc
     2341 tgcataatct cgaccataga cacctgtttc tcggccatgt ctattgcggc acccaccctg
     2401 gcggaatggc cggtccaggt cctatacctc cctttattgg gggtggcgtc ccgtttgttc
     2461 agcagcaccc aggcatcact gaaaatcttc tccatggcag gggcggtcag gggcgtggta
     2521 gtaatgcggg ctttgtttga ccggtgtata ggtgggaaga gcacagcgtc tggatgttct
     2581 ctaagcccac tgacgtccag ccagtcattg agcactgctg tggttcgccg ggagagcact
     2641 ttgtcaaggc ccgcagctgt ggtgattgtc ttagtgtgac tgatgtgcag ggtcactgtg
     2701 tctcctgtct gatccaagtc cccaaccctg atgcgtgata tttcagacat tctcatgagg
     2761 gtgttatagg ctacgaacag gaaagccctg ttgcgcaggt cgaccagccg ctcgctcctg
     2821 ctgagcagca catccagcag cttcaggtcg tcccagcgca gggggattgc ctgtcctgtc
     2881 ctctcaccct tttcggttgc ggcttctctt ctaattcttc tcatggcaag actcactgac
     2941 ttgtcgtcgg acaagggtgg cagtccgcag tgggacagaa gcatattaag catagcatag
     3001 tgcttgtcga tggtagtgga ggccaggtct gcatcgtgca actgaagaaa gtattcgcgt
     3061 gccatttcag gactaattgg aaaccatgca agctgccgag catggcacca tctggcccag
     3121 ctatgaaaga ccaacctcaa atccctcaaa gtgttaggag cgtacgcccc ctggtcattc
     3181 ataaacctca tgaagttttc agcggcctcc tgatactctt tgccgatgtt tcgcaggaat
     3241 ccaccagatg aaccactaat aatcagctca gaggatccag cgtaatctgg aacatcgtat
     3301 gggtaaacct tcctcttctt cttaggcatg gtggcgaatt cgttgctgct tcgcggctgc
     3361 cgggtggatg gcggaggcga ggaggaggtg ttgcttctta cagcgcgact cttaagcggt
     3421 cgatgtaaga gaacctgcgg cacttgggcg gatgcgcctc cttatataca gctcggtcac
     3481 catggggatg ggccaaggcc ttttcctgtt ggtccagacc cctccgtctg cccagagaag
     3541 ccgcgacagg agagtggtga ttggtgcaag cgacatggta tgaggtaatc tctcccccct
     3601 tccccccctc gctagcatcc gtagattttg catccttcag acaaaggcct cgcaaaagca
     3661 actagccgag catccattat ggggtggagc tggggagggg tagagaggga agaaagaaaa
     3721 cgggggttgg gtgattatac ggagcttaaa ccttaaactg tagttaggat aatttaaggt
     3781 tttccccaac acttaatcac agaatttgtg ttgagattct ttatggtaaa ttccagcttt
     3841 tcatttatac tacactaaat ttaaatacgt tccgtcagga ttcaacatct ctgttcgctt
     3901 taaataaagt agcaagagct atgggaatga tggtcaaaaa ggcgtgggta aatgtttagt
     3961 tttacagttg tattattgtg gttattatta ttactattat tattaatatt tgagcaaaat
     4021 tcagaagctc agtatgaata gaccacgcct atgaccctca acagcacccg ggaggcagag
     4081 gcaggaagac ttgcagctca aggtcatcat aaccgccctt agtggtttat gcctgtaaat
     4141 cccagcattg ggaagcggag gactgcggtt attttgtacc cggcctggat atatagtgtg
     4201 atatcctgtc tcaaaaacca aaaacaaaca aagagagaga gagagagaaa ggaaaaaaga
     4261 gaaacaatat acaaagtagt acttaacgct ttactagaga gcttgctttc tttagcggta
     4321 gtgctctccc atcacccctc cccgctcgcc cccttacccg accctacctc accccagagc
     4381 ctgtgttatt ttgtccctag aaatgagcca tccctcctaa tacggaatga tccccatcaa
     4441 gctgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc
     4501 cctcgacctg cagcctccgc agcccccaag ctagcagcca ccatggccag ctcaggggca
     4561 aaggcacaat gggttgggcc aagcctgggg caagggcccc gaaggcggcg ctgggcttgg
     4621 gctgaggaac aagatacaga tggaagaaga gaccagggct ggggaaacag ccagtctctt
     4681 cccgaagccc ccagtcctga gctcctagag gacttccgtc gggcacagga gcacctgcca
     4741 cccctggagt gggacccaga catgcaggac tccgaagagt cttctggaga agagaccgaa
     4801 gctgacgatg caagcagccc agagggttcc accgtgcctc tgccctggct ctccaggagc
     4861 aaccaacagc tggacatgtc tgaagaggag ctggatgagg cctctgggag gcctgaggta
     4921 gacttagctg gagagagttg tacggagctt gagtgtgaag accagggaga ttccagccct
     4981 ccacctccag gacaggggcc agccaaaggc tgggtcacct ttatcaaaca aggcagtaac
     5041 tacagacctt cagagcatct cgaggcccag ccatctgttg aacatagccg cacaaagtct
     5101 tggagcagtg gaactgtgag cctccgtcaa cctagtgaca gccttggttc tacctgggaa
     5161 ggagataccg aagtccctca gccttccatc ttgcccaaag ccctgccaca gagtccgtgc
     5221 cacaactttc cacacccagg tgacagaaat ggaggtgacg ttgctccggc aacacccaca
     5281 gaattccggg actccttagc agcgccagcc cagaatgccg agtgctctgc aggcacgtgg
     5341 ggggaagaaa ccaccagcct acccagctct cggcctgagg accaaacctg gaagaggact
     5401 aagacatcac ctaagccact gccctcccgg tttactggtt ctgtcagccc cctgagtact
     5461 cggcttgggg caataaagaa agttgtgccc cagcacaagc agggagccac tctggctggt
     5521 cactcttctt cacaagctcc caagtatggc agggggcgcc gcctgaacta cccactcccc
     5581 gacttctcca aggtggggcc ccgggtgagg tttcccaaag atgagaacta ccgacccccg
     5641 aagtccagag gccacaacag gcaacaaggc tctaccagac ccctcatctt caagtcgcca
     5701 gctgagattg ttcgggatgt actgttgagt agtggagagg cgtccctggc caaggaatct
     5761 tctcttgctc acaccatcac cagggtgccc caggagttcc agacacccga acaagccact
     5821 gagctagttc atcagctcca ggaggactat cacaagctcc tcaccaagta tgcggaggct
     5881 gagaacacta tcgaccaact tcgccttggg gccaaggtgc acctgtattc agacccacca
     5941 cagcccagtc agagcttctg ctcggggtca atgccacaag gaagcaaggt cttatccttc
     6001 tctatcccgc aacccagagt agcagagtgg tggcctgacc ctgcccagga tccacaggcc
     6061 tctgaggcca cagggtggcc gttcccacga acagacctga gtccctcctc gtcccccggc
     6121 gtggccactc cagggcggct tcctcagagc cagggcattg ccacagacca gccctccaca
     6181 ggacagaccc aggcactgac ctctcaggcc agccggctcc tggccaaggt gcagtccttc
     6241 gaggaactgg tactggctgg acacctccca cctcaggacc aaatcaagag cttggagcaa
     6301 ctgagggcag cccacatggc cctagaggca gagtacctgc aggcctgcag agaagagctc
     6361 ctggaccctc agcttgatgc ctctcagggg agtcccagga ccctcaacct ctgcagggag
     6421 ctagaagcag agatatacca cctgggacag cgcctggaag agctacagga ccatatggac
     6481 cagacccaaa gagagacaga gccatgtagg ccagacctgc aagacagcac cccaaccatg
     6541 tcctttctgc cccagtcagc tcatctgtcc atgccttcag gacctgtctc cttgccagat
     6601 ggccaaacct accaggagcc tgccaccacc accaccacct cccctggaag ctcttgcacg
     6661 ctccccatca acaagaagtt aagcctgagc atcaaaactg aggaaagccc aagaggtctc
     6721 ccggtcccac tcagggacag gacgcttcag gtggagcagg acttccatgg ccttctggag
     6781 agatacctca gcgttaaatc cctccctgaa gccttgaggg atgaagatga ggatgacctg
     6841 gaggaggagg aagaggagca ggaccatcaa ggccccttgg aagttgatag tccagccaca
     6901 gctccaggga aaacagaagc cgtgagggta ccaccaggag agcgcccaac acaggctgag
     6961 gaaagccata gggatgctac ccaggaggat gaggaacaga tgggacctat gaagtcacca
     7021 gacttccggc catccatggc cagagatacg tacacaccag tcctggatac agctgaagtg
     7081 gctcaacggg gcaccaaagc catggtgtca catcagagca gtctgaccag cctggaagag
     7141 agcaggccct cagagctcct tcctcgaaaa gccctgcttc gggctggtgg gccccacact
     7201 gaggagccct ggatggtgtc accagagaca gacagtggct tcgtgggctc agaaaccagt
     7261 atagtgtcac ccttcaccca gaccccagag caccggctct cccatgtcag cacttcagga
     7321 ccatcagctc aacacctcac tgcctctgtg cctggtgata gaacctccca tcccaaggcc
     7381 aggggtctca tggttcccag aagagccact gagactggca tacccagaag ccgaacccag
     7441 cagcatttct ccagcctgag ctctcccggg agaggggcac agagctgcca cctagaggag
     7501 acatcagtgg ctaaaatagc ggttcccagg tctgagttca agagacagaa gcagatttcc
     7561 aaacagctcc ttcccagtgg aagaaccagc cccgactctg ccccagctcc cacagctgcc
     7621 tccacgcccc atggatcagc agagagcact gccaacctcc tcctcaacag gacagagcgc
     7681 gaccaggcca tcaaggatct gcaggcggag gtgtcacggc tccgccttca actggaggac
     7741 agcctgcacc ggccacaccc agacggccca gcctgtgtag cttctgcttt caaccactcc
     7801 acccagaccc aggaaaagct gggttcctca ccctcctggg gcccccacta tggcagtaaa
     7861 tccacagaga ggctgtctag ggagcctaat ggtgtagagc cagctgagcc aatgggaaga
     7921 cgtcgagcca ggtcctcttc ggtgcctcgg gatgtaccca gactgtacct aagttctgaa
     7981 tcggagtcgc ctgcccctcg gctctcatct gagaagagca ggacctttga ggagcacccc
     8041 gaggctgcac agtgggggac aagaccacag agcagctcga agcggaggga aagagtttcc
     8101 ttccggggtc aatacacagg ccaggagtac cacattctat ccccaaaggc catcctgaag
     8161 gacagtggca caccgtcctg tccccactgc catcccatta ggacccagga cacaggcagt
     8221 gctgtctcta gagacaccac acggggctca tctgccgctg acaccctccg ctgtgccctg
     8281 tgtggtgaag tcaagtcctc tgcagaagca gatggctcca gctcaggtcc ctctgagaag
     8341 aacaccccca aaaagccttc cacccccatc ctcaaacgga agaacaggca gacgggctcg
     8401 ccagtacgaa tggcccctgg actctggtac ctggctgctg cgcccccagc cccagcccca
     8461 ccggctttgg cctacatctc ctcagctccc attatgcctt atctaccacc cactgtgtac
     8521 tatgcagccc cagcacctac ctcagcccag acagcttccc cgcagcctgc tcggggacct
     8581 cggagaaccc ggcactcagt ccaactgggt ctgaatgacc tggaggagct gcaggccgca
     8641 ctcagagagg ctgcacaggc cgccgagaac gttcgctcca ccacccgcca gctgagccgc
     8701 tccctgtctg cggacctgcg ccatgcccgt agcctgcgag gctcctgcct tttcgtcgac
     8761 ggcagcaagc ttggtggagg cagcgcatca ggcggaagcg gaagcagatc tatggtgagc
     8821 aagggcgagg agaataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcgcatg
     8881 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     8941 gagggctttc agaccgctaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     9001 gacatcctgt cccctcattt cacctacggc tccaaggcct acgtgaagca ccccgccgac
     9061 atccccgact acttcaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     9121 tacgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     9181 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt gatgcagaag
     9241 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg tgccctgaag
     9301 ggcaagatca agatgaggct gaagctgaag gacggcggcc actacacctc cgaggtcaag
     9361 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt cgacatcaag
     9421 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     9481 ggccgccact ccaccggcgg catggacgag ctgtacaagt aagcggccgc catatgcatc
     9541 ctaggcctat taatattccg gagtatacgt agccggctaa cgttaacaac cggtacctct
     9601 agataagtaa tgagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     9661 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     9721 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     9781 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     9841 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gagatccact
     9901 agttctagcc tcgaggctag agcggccgcc actggccgtc gttttacaac gtcgtgactg
     9961 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    10021 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    10081 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    10141 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    10201 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    10261 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    10321 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    10381 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    10441 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    10501 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
    10561 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
    10621 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    10681 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    10741 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    10801 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    10861 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
    10921 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    10981 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    11041 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    11101 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    11161 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    11221 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    11281 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    11341 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    11401 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    11461 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    11521 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    11581 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    11641 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    11701 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    11761 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    11821 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
    11881 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    11941 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    12001 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    12061 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    12121 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    12181 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    12241 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    12301 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    12361 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    12421 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    12481 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    12541 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    12601 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    12661 agcggataac aatttcacac aggaaacagc tatgaccatg aggcgcgccg
//
