LOCUS       pCAG-lox-inv[Ta1       11457 bp DNA     circular SYN 06-MAY-2022
DEFINITION  pCAG-loxPSTOPloxP-Lyn-EGFP.
ACCESSION   pCAG-loxPSTOPloxP-ZsGreen
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     Backbone is pCAG-loxP-inv[Ta1-Dre-pA]-loxP-Lyn-EGFP 
            [HindIII/BamHIII]Backbone was digested with with 
            PstI/SacI2xHA-Camsap3 was generated as follows:Template pCMV-3xHA-C1
            and primers2-Flank-2HA-Camsap fw 
            5'-AGGTCCCTCGACCTGCAGCTGCAGGCCACCATGTACCCATACGATGTTCC-3'2xHA-tag-lin
            ker rv
            5'-ACTAGAACCTCCTGAACTTCCACCTGCATAGTCCGGG-3'PCR product 1 
            (111bp)Template Camsap3 plasmid from Max and primers:Linker-CAMSAP3 
            fw
            5'-GACTATGCAGGAAGTTCAGGAGGTTCTAGTATGGTGGAAGC-3'CAMSAP3-SacI rv
            5'-GTCGAGGCTGATCAGCGAGCTCTTATTTGGGGGTACCGCCAC-3'PCR product 2 
            (3800bp)PCR 1 and 2 were LIC cloned into the above PstI/SacI 
            linearized vector.Resistance= AmpicilinVNTEXTCHREPL|Animal/Other 
            Eukaryotic
            VNTREPLTYPE|Plasmid
FEATURES             Location/Qualifiers
     source          1..11457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    86..1536
                     /label=CAG promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    86..373
                     /label=CAG_enhancer
     CDS             complement(805..1536)
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MEKGTAARRRTKALPAHFLPAGAAPLRPRPLEGVRRRLPDFGSAR
                     FGTKEVPAPSRTITIKGNGCGSPRLDSRRRSGAAAPLPRALPGPRRPRPARHPHLPPPP
                     ARHGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSPR
                     SPPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPPH
                     KGPSRSPLRLSRSHRKETSRH"
     CDS             complement(846..1349)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPGT
                     ASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAAPP
                     HRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRTKGP
                     PGAL"
     promoter        1686..1704
                     /label=T7_promoter
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     misc_feature    complement(1733..1766)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     polyA_site      complement(1789..2237)
                     /label=rabbit_polyA_terminator
                     /note="/type=polyA_site"
                     /note="/vntifkey=26"
     misc_feature    complement(2247..3329)
                     /label=NLS-HA-Dre
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(3342..4429)
                     /label=Tub-alpha-1 promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    complement(4461..4494)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     misc_feature    4521..8360
                     /label=2xHA-Camsap3
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    4524..4550
                     /label=HA tag
                     /label=nonstandard type: misc_marker
                     /note="/type=misc_marker"
                     /note="/vntifkey=22"
     misc_feature    4554..4580
                     /label=HA tag
                     /label=nonstandard type: misc_marker
                     /note="/type=misc_marker"
                     /note="/vntifkey=22"
     misc_feature    4581..4601
                     /label=linker
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    4602..8360
                     /label=Camsap3
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    8361..8366
                     /label=Sac I
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(8376..8393)
                     /label=BGH_rev_primer
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
     terminator      8379..8606
                     /label=bGH_PA_terminator
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
     CDS             8580..9053
                     /codon_start=1
                     /label=ORF frame 2
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSV
                     TATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD"
     promoter        complement(8678..8694)
                     /label=M13_forward20_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    8687..8835
                     /label=lacZ_a
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(8687..8709)
                     /label=M13_pUC_fwd_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     rep_origin      8853..9159
                     /label=f1_origin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        9352..9380
                     /label=AmpR_promoter
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     gene            9422..10282
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             9422..10282
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10437..11056
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        11365..11394
                     /label=lac_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    11408..11430
                     /label=M13_pUC_rev_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        11429..11447
                     /label=M13_reverse_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
      841 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta
     1681 attaataata cgactcacta tagggggtcg acccgccacc aatcatttaa atataacttc
     1741 gtataatgta tgctatacga agttattcgc gatgaataaa tgaaagctgt cgagggatct
     1801 tcataagaga agagggacag ctatgactgg gagtagtcag gagaggagga aaaatctggc
     1861 tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca caaaacaaaa
     1921 tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat ggacagcagg
     1981 gggctgtttc atatactgat gacctcttta tagccacctt tgttcatggc agccagcata
     2041 tggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa tgatttgccc
     2101 tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt gcaatgaaaa
     2161 taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg tccccataat
     2221 ttttggcaga gggaaaaaga tctgactcag ctatccatca gtcgagaatt ggctcccacg
     2281 gacactccgc cccttctgag ataccgcatc agtgtttcag gcttcttcca tgtcccttcc
     2341 tgcataatct cgaccataga cacctgtttc tcggccatgt ctattgcggc acccaccctg
     2401 gcggaatggc cggtccaggt cctatacctc cctttattgg gggtggcgtc ccgtttgttc
     2461 agcagcaccc aggcatcact gaaaatcttc tccatggcag gggcggtcag gggcgtggta
     2521 gtaatgcggg ctttgtttga ccggtgtata ggtgggaaga gcacagcgtc tggatgttct
     2581 ctaagcccac tgacgtccag ccagtcattg agcactgctg tggttcgccg ggagagcact
     2641 ttgtcaaggc ccgcagctgt ggtgattgtc ttagtgtgac tgatgtgcag ggtcactgtg
     2701 tctcctgtct gatccaagtc cccaaccctg atgcgtgata tttcagacat tctcatgagg
     2761 gtgttatagg ctacgaacag gaaagccctg ttgcgcaggt cgaccagccg ctcgctcctg
     2821 ctgagcagca catccagcag cttcaggtcg tcccagcgca gggggattgc ctgtcctgtc
     2881 ctctcaccct tttcggttgc ggcttctctt ctaattcttc tcatggcaag actcactgac
     2941 ttgtcgtcgg acaagggtgg cagtccgcag tgggacagaa gcatattaag catagcatag
     3001 tgcttgtcga tggtagtgga ggccaggtct gcatcgtgca actgaagaaa gtattcgcgt
     3061 gccatttcag gactaattgg aaaccatgca agctgccgag catggcacca tctggcccag
     3121 ctatgaaaga ccaacctcaa atccctcaaa gtgttaggag cgtacgcccc ctggtcattc
     3181 ataaacctca tgaagttttc agcggcctcc tgatactctt tgccgatgtt tcgcaggaat
     3241 ccaccagatg aaccactaat aatcagctca gaggatccag cgtaatctgg aacatcgtat
     3301 gggtaaacct tcctcttctt cttaggcatg gtggcgaatt cgttgctgct tcgcggctgc
     3361 cgggtggatg gcggaggcga ggaggaggtg ttgcttctta cagcgcgact cttaagcggt
     3421 cgatgtaaga gaacctgcgg cacttgggcg gatgcgcctc cttatataca gctcggtcac
     3481 catggggatg ggccaaggcc ttttcctgtt ggtccagacc cctccgtctg cccagagaag
     3541 ccgcgacagg agagtggtga ttggtgcaag cgacatggta tgaggtaatc tctcccccct
     3601 tccccccctc gctagcatcc gtagattttg catccttcag acaaaggcct cgcaaaagca
     3661 actagccgag catccattat ggggtggagc tggggagggg tagagaggga agaaagaaaa
     3721 cgggggttgg gtgattatac ggagcttaaa ccttaaactg tagttaggat aatttaaggt
     3781 tttccccaac acttaatcac agaatttgtg ttgagattct ttatggtaaa ttccagcttt
     3841 tcatttatac tacactaaat ttaaatacgt tccgtcagga ttcaacatct ctgttcgctt
     3901 taaataaagt agcaagagct atgggaatga tggtcaaaaa ggcgtgggta aatgtttagt
     3961 tttacagttg tattattgtg gttattatta ttactattat tattaatatt tgagcaaaat
     4021 tcagaagctc agtatgaata gaccacgcct atgaccctca acagcacccg ggaggcagag
     4081 gcaggaagac ttgcagctca aggtcatcat aaccgccctt agtggtttat gcctgtaaat
     4141 cccagcattg ggaagcggag gactgcggtt attttgtacc cggcctggat atatagtgtg
     4201 atatcctgtc tcaaaaacca aaaacaaaca aagagagaga gagagagaaa ggaaaaaaga
     4261 gaaacaatat acaaagtagt acttaacgct ttactagaga gcttgctttc tttagcggta
     4321 gtgctctccc atcacccctc cccgctcgcc cccttacccg accctacctc accccagagc
     4381 ctgtgttatt ttgtccctag aaatgagcca tccctcctaa tacggaatga tccccatcaa
     4441 gctgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc
     4501 cctcgaccct gcaggccacc atgtacccat acgatgttcc agattacgct ggctatccct
     4561 atgacgtccc ggactatgca ggaagttcag gaggttctag tatggtggaa gcggcgcccg
     4621 cagggtccgg gccactgcgg aggactttcc tggtccccga gatcaagtct ttggaccagt
     4681 acgatttctc tcgagccaag gcggcggcga gtctggcgtg ggtgctgcgg gccgcattcg
     4741 ggggagcaga gcatgtgcct ccggagctgt gggaaccctt ctacaccgat cagtacgcac
     4801 aggagcacgt gaagccccca gtgacgcggc tgctgctctc tgcggaacta tactgtcggg
     4861 cctggcgcca ggcactgcca cagctcgagc catcccccag tccctctgct ctgttggcct
     4921 tgctggcgag gaggggcacg gtgccttcac tgcctgagca cccagtgcgc gaggctgacc
     4981 tgaaacacca gccaattctc atgggagccc acctagctgt catcgatgct ctcatggttg
     5041 ccttctcatt tgagtggaca aagacactac ctggtccctt ggctctgagc agcttggagc
     5101 acaaactcct tttctgggta gacacaactg ttcggcggct gcaggagaag acagaacaag
     5161 aagcagccca gcgtgcatct cctgcagccc ctctggatgg ggcctctcct gcccagccct
     5221 cgattcgata tcgcaaggac cgtgccattg cccgaagggc tccctgcttt ccaaatgtga
     5281 ctacccttca ggacctggcc agtggggcag cactggctgc caccatccac tgctattgtc
     5341 cccagctatt acgacttgag gaggtgtgcc tcaaggaccc catgtctgtg gcagacagtc
     5401 tatacaacct ccagctggtg caagacttct gtgcctccca tcttcctcga ggctgccccc
     5461 tgtcccttga agacttgctg tatgtcccac cacccctcaa ggtcaacctg gtggtgctgt
     5521 tggctgagat gtatatgtgc tttgaggttc tgaagcctga ttttgtgcag gccaaagact
     5581 tgcctgatgg acatgctgtc tccccccgga atacagagac tgttccatct cagaacaaca
     5641 gtggcagcag ttctcctgtc ttcaacttcc gtcacccact tctgtcacct ggtggtcccc
     5701 agtctccact ccgaggatcc acaggctccc tgaagtcctc tccatcaatg tctcacatgg
     5761 aggctcttgg caaagcctgg aaccgtcagc ttagccgtcc cctctcccag gctgtgtcgt
     5821 tcagcactcc ctttggcctg gacagcgatg tggatgtcgt catgggagat cctgtcctgc
     5881 tccgctccgt cagctcagac agtctgggtc ctccacgtcc tgtgtcaaca tcatcccgga
     5941 attctgctca gccagcccca gaatctggag acctacccac gattgaagag gccctgcaga
     6001 tcattcacag tgctgagccc cgactgctcc ctgatggggc tgctgatggc agtttctacc
     6061 tccattctcc tgagggtctc tccaaaccac cactctcccc ctaccctccc gaaggagcct
     6121 caaagccgct gtctgatagg ctcaacaagg cgcctatcta tatatcacac cctgagaacc
     6181 cttcaaaatc atctccctgc tcaacaggag agatactgaa accaccaccc ccatccgagg
     6241 gttcccccaa agctgtggct tcatccccag cagccaacaa ctccgaagtg aagatgacca
     6301 gttttgctga acgcaagaaa cagctggtga aggctgaggc tgagtcagga ttggggtctc
     6361 caacatccac ccccgtagca cctgaggcct tgagctcaga gatgagtgag ctgggagcca
     6421 ggctggagga gaagcgccga gccatagagg cacagaaacg acgcattgag gcaatctttg
     6481 ccaagcatag gcagaggctg ggcaagagcg ctttcctgca ggtgcagcct cgggaggctg
     6541 caggggaggc cgaggaggaa gctgagctgg gctcagttcc tggtggggaa cggccagcag
     6601 gtgagggcca gggtgagccg tccttacggc acaagtcagt taccttctct ccagacctgg
     6661 gcccagtgcc cccagaggga cttggggatt acaatagagc agtcagtaag ctgagtgccg
     6721 ctctgagctc tctgcagcgg gacatgcaga ggctcacaga ccagcaacag cggcttctag
     6781 cccctccaga agctcctgga cctgccccac cacctgcagc ctgggtcatt cctggacccg
     6841 ccactgggcc taaagcagca tcccccagcc ctgcccgtcg tgccccagct gcccgacgca
     6901 gccctgggcc aggccccagc ccaacacccc gtagtccaaa acatgcaagg ccggcagagc
     6961 tgaagcttgc acctttgaca agggtactca caccacccca tgatgtagac agcctccctc
     7021 acctacgcaa gttttcaccc agccaggtgc ctgtacagac tcgctcctct atcctcctgt
     7081 cggaggggac acctcccgag gagcccacca ccaagcctgc cctcattgag atccctctag
     7141 ccagcctggg ggagcctgct gctgatgagg aaggagatgg gagcccccct ggggctgagg
     7201 attccttaga ggaagaggca tcttctgagg gagagccccg atcagggctt ggattctttt
     7261 ataaggacga agacaagcct gaggatgaga tggctcagaa gcgagctagc ctgctggagc
     7321 gtcagcagag gcgggtagag gaagcccggc ggcgcaaaca gtggcaggag gcagagaagg
     7381 agcagaaacg ggaggaggcc gccaggctgg ctcaggaggc tccaggcttg gcctttacaa
     7441 ctcctgttgt agcctctgcg gctccagtgg ccaccttggc tcctactacc agagccatgg
     7501 ccccagctga ggaggaggtg ggcccccggc ggggggactt cacaagactt gagtatgaac
     7561 gccgggcaca actgaaactg atggatgacc ttgataaggt gctacggccc cgggcctcag
     7621 ggaccggggg accagggcgg ggcgggcgca gggccacccg gccacgctct ggttgctgtg
     7681 atgactcggc cttggcacga agcccagccc gcggcctgct gggttcacgg ctcagcaagg
     7741 tctattccca gtccacgctg tccctatcta cggtggccaa cgaggctccc aataaccttg
     7801 gtgtgaagag gcccacctct cgggcccctt ctccatcagg cctcatgtct ccaagccgcc
     7861 tgcctggcag tcgagaacgt gactgggaga atggaagcaa tgcatcctcc ccagcatcag
     7921 tgcctgagta cacaggtccc cggctataca aggaacccag cgccaagtct aacaagttta
     7981 tcatccacaa tgccttgtca cactgctgcc tggcaggcaa ggtgaatgag ccccagaaga
     8041 acagaattct agaggaaatc gagaaaagca aggccaacca cttcctgatt ctctttcggg
     8101 actcaagctg ccagttccgg gccctctaca ctctgtctgg ggagacagag gagctatcga
     8161 ggctggcagg ctatgggcct cggaccgtca ctcctgccat ggtcgaaggc atctataagt
     8221 acaactcgga tcgcaaacgg ttcacccaga tccctgctaa aaccatgtct atgagtgtag
     8281 acgccttcac tatccaggga cacctttggc aaagcaagaa gcccaccacg cccaagaagg
     8341 gtggcggtac ccccaaataa gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     8401 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     8461 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     8521 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     8581 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
     8641 atccactagt tctagcctcg aggctagagc ggccgccact ggccgtcgtt ttacaacgtc
     8701 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     8761 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     8821 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     8881 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8941 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     9001 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     9061 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     9121 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     9181 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     9241 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     9301 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     9361 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     9421 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     9481 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     9541 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     9601 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     9661 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     9721 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     9781 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     9841 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9901 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9961 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    10021 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    10081 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    10141 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    10201 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    10261 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    10321 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    10381 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    10441 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    10501 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    10561 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
    10621 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    10681 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    10741 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    10801 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    10861 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    10921 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10981 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    11041 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    11101 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    11161 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    11221 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    11281 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    11341 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    11401 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgagg cgcgccg
//
