LOCUS       pCAG-lox-inv[Ta1        8548 bp DNA     circular SYN 01-AUG-2022
DEFINITION  pCAG-lox-inv[Ta1-iCre-pA]-lox-2xHA-linker-AKAP9(128-425).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
COMMENT     pCAG-loxP-inv[Ta1-iCre-pA]-loxP-2xHA-hCentrin was linearized with 
            KpnI/SacI to release hCentrin
            AKAP9(128-425) was amplified from pCAG-Halotag-AKAP(128-425) with 
            primers:  
            2xHA-AKAP9 mut fw
            5'-ggaggttctagtggtacctccggaggtggtggaggt-3'
            AKAP9 rv
            5'-GGCTGATCAGCGAGCTCTCAAAGCTCCTCTATCTG-3'
            PCR product (aprox. 900 bp) 3 was LIC cloned into the above 
            KpnI/SacI linearized vector.
            Resistance= Ampicilin
FEATURES             Location/Qualifiers
     source          1..8548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    86..1536
                     /label=CAG promoter
                     /label=nonstandard type: eukaryotic
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    86..373
                     /label=CAG_enhancer
                     /note="/vntifkey=21"
     CDS             complement(805..1536)
                     /codon_start=1
                     /label=ORF frame 3
                     /note="/vntifkey=4"
                     /translation="MEKGTAARRRTKALPAHFLPAGAAPLRPRPLEGVRRRLPDFGSAR
                     FGTKEVPAPSRTITIKGNGCGSPRLDSRRRSGAAAPLPRALPGPRRPRPARHPHLPPPP
                     ARHGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSPR
                     SPPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPPH
                     KGPSRSPLRLSRSHRKETSRH"
     CDS             complement(846..1349)
                     /codon_start=1
                     /label=ORF frame 1
                     /note="/vntifkey=4"
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPGT
                     ASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAAPP
                     HRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRTKGP
                     PGAL"
     promoter        1686..1704
                     /label=T7_promoter
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
                     /note="/vntifkey=30"
     misc_feature    complement(1733..1766)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
                     /note="/vntifkey=21"
     polyA_site      complement(1784..2214)
                     /label=rabbit_polyA_terminator
                     /note="/type=polyA_site"
                     /note="/vntifkey=26"
     misc_feature    complement(2221..3276)
                     /label=NLS-iCre
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(3289..4376)
                     /label=Tub-alpha-1
                     /label=nonstandard type: eukaryotic
                     /note="Tubulin-alpha-1 promoter"
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
                     /note="/vntifkey=29"
     misc_feature    complement(4392..4425)
                     /label=loxP
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
                     /note="/vntifkey=21"
     misc_feature    4439..4444
                     /label=Pst I
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    4450..5451
                     /label=2xHA-2xlinker-AKAP9(128-425)
     misc_feature    4453..4479
                     /label=HA tag
                     /label=nonstandard type: misc_marker
                     /note="/type=misc_marker"
                     /note="/vntifkey=22"
     misc_feature    4483..4509
                     /label=HA tag
                     /label=nonstandard type: misc_marker
                     /note="/type=misc_marker"
                     /note="/vntifkey=22"
     misc_feature    4510..4530
                     /label=linker
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    4537..4554
                     /label=linker
     misc_feature    4555..5451
                     /label=AKAP9(128-425)
     misc_feature    5452..5457
                     /label=Sac I
                     /note="/type=misc_feature"
                     /note="/vntifkey=21"
     misc_feature    complement(5467..5484)
                     /label=BGH_rev_primer
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
                     /note="/vntifkey=21"
     terminator      5470..5697
                     /label=bGH_PA_terminator
                     /note="/translation=MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFK
                     GKQAI 
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG 
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI 
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH 
                     LTEHAIASGSALP*"
                     /note="/vntifkey=43"
     CDS             5671..6144
                     /codon_start=1
                     /label=ORF frame 2
                     /note="/vntifkey=4"
                     /translation="MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQRRDWEN
                     PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSV
                     TATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD"
     promoter        complement(5769..5785)
                     /label=M13_forward20_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=30"
     misc_feature    5778..5926
                     /label=lacZ_a
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=21"
     misc_feature    complement(5778..5800)
                     /label=M13_pUC_fwd_primer
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=21"
     rep_origin      5944..6250
                     /label=f1_origin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=33"
     promoter        6443..6471
                     /label=AmpR_promoter
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=30"
     gene            6513..7373
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MLGMRWALWLLRRKEPAGARDPLVLASRLERPPLAVVLQ
                     RRDWE 
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGALSAAGVVVTR 
                     SVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
                     /note="/vntifkey=60"
     CDS             6513..7373
                     /codon_start=1
                     /label=ORF frame 1
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7528..8147
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="/vntifkey=33"
     promoter        8456..8485
                     /label=lac_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="/vntifkey=30"
     misc_feature    8499..8521
                     /label=M13_pUC_rev_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="/vntifkey=21"
     promoter        8520..8538
                     /label=M13_reverse_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
                     /note="/vntifkey=30"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
      541 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
      841 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta
     1681 attaataata cgactcacta tagggggtcg acccgccacc aatcatttaa atataacttc
     1741 gtataatgta tgctatacga agttattcgc gatgaataaa tgagagaaga gggacagcta
     1801 tgactgggag tagtcaggag aggaggaaaa atctggctag taaaacatgt aaggaaaatt
     1861 ttagggatgt taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc taacctcaag
     1921 tcaaggcttt tctatggaat aaggaatgga cagcaggggg ctgtttcata tactgatgac
     1981 ctctttatag ccacctttgt tcatggcagc cagcatatgg catatgttgc caaactctaa
     2041 accaaatact cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag
     2101 agacacaaaa aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa
     2161 gtcagatgct caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct
     2221 tcagtcccca tcctcgagca gcctcaccat ggccccagtc tcagagtcca ggtttctgat
     2281 gtagttcatc acaatgttca cattggtcca gccaccagcc tgcatgattt cagggatgga
     2341 cacaccagcc ctggccatgt ccctggcagc acccactctg gcagagtggc cagaccaggc
     2401 caggtatctc tgcccagagt catccttggc accatagatc aggcggtggg tggcctcaaa
     2461 gatcccttcc agggcccggg tggacagttg ggaggtggca gaaggggcag ccacaccatt
     2521 ctttctgacc cggcagaaca ggtagttgtt ggggtcatca gccacaccag acacagagat
     2581 ccatctctcc accagcttgg taacccccag ggacagggcc ttctccacac cagctgtgga
     2641 caccagggtc ttggtcctgc caatgtggat cagcattctc ccaccatcgg tgcgggagat
     2701 gtccttcact ctgattctgg caatttcggc aatgcgcagc agggtgttgt aggcaatgcc
     2761 caggaaggcc aggttcctga tgtcctggca tctgtcagag ttctccatca gggatctgac
     2821 ttggtcaaag tcagtgcgtt caaaggccag ggcctgcttg gctctctccc cagcatccac
     2881 attctccttt ctgattctcc tcatcaccag ggacacagca ttggagtcag aagggcgagg
     2941 caggccagat ctcctgtgca gcatgttgag ctggcccagg tgctgttgga tggtcttcac
     3001 agccaggcct ctggcttgca ggtacaggag gtagtccctc acatcctcag gttcagcagg
     3061 gaaccatttc ctgttgttca gcttgcacca ggcagcccag gatctgcaca cagacaggag
     3121 catcttccag gtgtgttcag agaaggcctg cctgtccctg aacatgtcca tcaggttctt
     3181 cctgacttca tcagaggtgg catccacagg gagggcaggc aggttttggt gcacagtcag
     3241 caggttggag actttcctct tcttcttggg caccatggtg gcgaattcgt tgctgcttcg
     3301 cggctgccgg gtggatggcg gaggcgagga ggaggtgttg cttcttacag cgcgactctt
     3361 aagcggtcga tgtaagagaa cctgcggcac ttgggcggat gcgcctcctt atatacagct
     3421 cggtcaccat ggggatgggc caaggccttt tcctgttggt ccagacccct ccgtctgccc
     3481 agagaagccg cgacaggaga gtggtgattg gtgcaagcga catggtatga ggtaatctct
     3541 ccccccttcc ccccctcgct agcatccgta gattttgcat ccttcagaca aaggcctcgc
     3601 aaaagcaact agccgagcat ccattatggg gtggagctgg ggaggggtag agagggaaga
     3661 aagaaaacgg gggttgggtg attatacgga gcttaaacct taaactgtag ttaggataat
     3721 ttaaggtttt ccccaacact taatcacaga atttgtgttg agattcttta tggtaaattc
     3781 cagcttttca tttatactac actaaattta aatacgttcc gtcaggattc aacatctctg
     3841 ttcgctttaa ataaagtagc aagagctatg ggaatgatgg tcaaaaaggc gtgggtaaat
     3901 gtttagtttt acagttgtat tattgtggtt attattatta ctattattat taatatttga
     3961 gcaaaattca gaagctcagt atgaatagac cacgcctatg accctcaaca gcacccggga
     4021 ggcagaggca ggaagacttg cagctcaagg tcatcataac cgcccttagt ggtttatgcc
     4081 tgtaaatccc agcattggga agcggaggac tgcggttatt ttgtacccgg cctggatata
     4141 tagtgtgata tcctgtctca aaaaccaaaa acaaacaaag agagagagag agagaaagga
     4201 aaaaagagaa acaatataca aagtagtact taacgcttta ctagagagct tgctttcttt
     4261 agcggtagtg ctctcccatc acccctcccc gctcgccccc ttacccgacc ctacctcacc
     4321 ccagagcctg tgttattttg tccctagaaa tgagccatcc ctcctaatac ggaatttccg
     4381 gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt ccctcgacct
     4441 gcagccacca tgtacccata cgatgttcca gattacgctg gctatcccta tgacgtcccg
     4501 gactatgcag gaagttcagg aggttctagt ggtacctccg gaggtggtgg aggtatgggg
     4561 gaaaacgagt tggctgagaa gcagcatgag gtcgaagagt tgactcagga gttggaagaa
     4621 atgagggcca gctttgggac ggaagggctg aagcagttac aagaattcga agccgccatt
     4681 aaacaaagag atggcatcat cactcagctc actgctaact tgcagcaagc cagaaaagaa
     4741 aaggatgaca caacgataga atttttagag ttgacagaac agagtcaaaa gctgcagatt
     4801 cagttccagc acttacaggc taatgaaacc ctgcaaaaca gcactcttag tcgaactgca
     4861 acagatttac tgcaggctaa acgacagatc tttactctgc agcaacagct gcaagattat
     4921 cagaaaaagg aggaggacgt ccaagcacaa atttcttttt tacaagagaa aattagaatg
     4981 ttggaaatgg aaaaagacag aaaagtggaa agcttaaata caaaagaaat agaggaaaag
     5041 caggcaatca ttgaggagtt gaatacaaga gtaatagaag aagagagaaa aactgctgag
     5101 ctagagaata aagtgacaac tgctgatgaa ttactaggac agctccaagg acaggttaca
     5161 gaaaaggatc aggagatcaa aagcctgaaa ctagaactgg tcaactctca gcagaacgag
     5221 cggaagtgtt cagaggaaat caaagagcta atgaggacag ttgaagaact ccagaagaga
     5281 aatctcaagg acagccagct ggggaccggc accgtgcgaa gggtggaaca agaaacacaa
     5341 cggaaactaa gccttctcca ggcagagctg gatgagatgt atgggaagca gatagtgcaa
     5401 atgaaacaag aattaatgaa acagcacatg tctcagatag aggagctttg agagctcgct
     5461 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     5521 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     5581 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     5641 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     5701 ctgaggcgga aagaaccagc tggggctcga gatccactag ttctagcctc gaggctagag
     5761 cggccgccac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     5821 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     5881 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     5941 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6001 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6061 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6121 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6181 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6241 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6301 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6361 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6421 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6481 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6541 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6601 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6661 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6721 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6781 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6841 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6901 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6961 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7021 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7081 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7141 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7201 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7261 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7321 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7381 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7441 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7501 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7561 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7621 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7681 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7741 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7801 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7861 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7921 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7981 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8041 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8101 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8161 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8221 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8281 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8341 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8401 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8461 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     8521 gaaacagcta tgaccatgag gcgcgccg
//
