LOCUS       pBactin_Halo-tag        6923 bp DNA     circular SYN 17-MAY-2022
DEFINITION  Gibson Assembly of 1_RhoA-BS_PCR#1 into Digestion of pCAG-AcGFP-CI 
            (modified) - Fragment 2.
ACCESSION   urn.local...r-ett3iip
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 13, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          5986..6011
                     /mol_type="other DNA"
                     /label=source pEGFP-C1
                     /note="/Transferred_From=pCAG-Cdc422G, pCAG-DORARhoA(Q63L),
                     pCAG-DORARhoA(wt), pCAG-RaichuCRRhoA, pCAG-mCherry-GEFH1, 
                     pCAG-mCherry-LFC, pCAG-mClover, pCAG-mClover-mRuby2, 
                     pCAG-mRuby2, pCAG-mRuby2-GEFH1, pCAG-mRuby2-LFC, 
                     pCAG-mRuby2-p190RhoGAP, pCAG-mTFP, pCAG-mTFP-GEFH1, 
                     pCAG-mTFP-LFC, pCAG-mTFP-mVenus, pCAG-mVenus, 
                     pCAG-meGFP-mCherry, pCAG-p190RhoGAP, 
                     pCAG_mCherry-p190RhoGAP"
                     /note="/Transferred_Similarity=100.00%"
                     /organism="pEGFP-C1"
     misc_feature    3..8
                     /label=KpnI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGTAC^C"
                     /note="/annotation_group=GGTACC"
     misc_feature    3..8
                     /label=Acc65I, Asp718I
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GTACC"
                     /note="/annotation_group=GGTACC"
     misc_feature    11..16
                     /label=ApaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGGCC^C"
                     /note="/annotation_group=GGGCCC"
     misc_feature    11..16
                     /label=Bsp120I, PspOMI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GGCCC"
                     /note="/annotation_group=GGGCCC"
     misc_feature    14..19
                     /label=Cfr9I, TspMI, XmaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^CCGGG"
                     /note="/annotation_group=CCCGGG"
     misc_feature    18..23
                     /label=BamHI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GATCC"
                     /note="/annotation_group=GGATCC"
     misc_feature    30..35
                     /label=XbaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=T^CTAGA"
                     /note="/annotation_group=TCTAGA"
     polyA_signal    43..271
                     /label=SV40 early polyA signal
                     /note="/Transferred_From=pCAG-mCherry-LFC"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/type=polyA_signal"
     misc_feature    43..48
                     /label=BclI, FbaI, Ksp22I
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=T^GATCA"
                     /note="/annotation_group=TGATCA"
     misc_feature    136..141
                     /label=MfeI, MunI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^AATTG"
                     /note="/annotation_group=CAATTG"
     primer_bind     complement(182..211)
                     
     primer_bind     complement(187..206)
                     
     primer_bind     241..260
                     
     primer_bind     complement(298..314)
                     
     misc_feature    639..644
                     /label=PciI, PscI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=A^CATGT"
                     /note="/annotation_group=ACATGT"
     misc_feature    953..958
                     /label=ApaLI, Alw44I, VneI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
                     /note="/annotation_group=GTGCAC"
     misc_feature    1359..1364
                     /label=BspHI, CciI, PagI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=T^CATGA"
                     /note="/annotation_group=TCATGA"
     CDS             complement(1459..2319)
                     /codon_start=1
                     /label=AmpR
                     /note="/Transferred_From=pCAG-mCherry-LFC"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/type=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    2199..2204
                     /label=ApaLI, Alw44I, VneI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
                     /note="/annotation_group=GTGCAC"
     misc_feature    2367..2372
                     /label=BspHI, CciI, PagI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=T^CATGA"
                     /note="/annotation_group=TCATGA"
     misc_feature    2450..2455
                     /label=AatII
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GACGT^C"
                     /note="/annotation_group=GACGTC"
     misc_feature    2472..2477
                     /label=BspHI, CciI, PagI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=T^CATGA"
                     /note="/annotation_group=TCATGA"
     primer_bind     complement(2549..2567)
                     /label=pGEX-3
     misc_feature    2696..2701
                     /label=ApaLI, Alw44I, VneI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
                     /note="/annotation_group=GTGCAC"
     misc_feature    2754..2759
                     /label=PluTI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGCGC^C"
                     /note="/annotation_group=GGCGCC"
     misc_feature    2754..2759
                     /label=KasI, SspDI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GCGCC"
                     /note="/annotation_group=GGCGCC"
     misc_feature    2860..4992
                     /label=chicken Beta-actin
                     /label=nonstandard type: eukaryotic
                     /note="/Transferred_From=pCAG-mCherry-LFC"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/promoter_type=eukaryotic"
                     /note="/type=eukaryotic"
     primer_bind     2897..2914
                     /label=M13-FP
     primer_bind     2897..2914
                     
     misc_feature    3079..3084
                     /label=AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I,
                     MroI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=T^CCGGA"
                     /note="/annotation_group=TCCGGA"
     misc_feature    3162..3167
                     /label=ApaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGGCC^C"
                     /note="/annotation_group=GGGCCC"
     misc_feature    3162..3167
                     /label=Bsp120I, PspOMI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GGCCC"
                     /note="/annotation_group=GGGCCC"
     misc_feature    3497..3502
                     /label=PstI, BspMAI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CTGCA^G"
                     /note="/annotation_group=CTGCAG"
     misc_feature    3545..3550
                     /label=PstI, BspMAI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CTGCA^G"
                     /note="/annotation_group=CTGCAG"
     misc_feature    3733..3738
                     /label=SphI, PaeI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
                     /note="/annotation_group=GCATGC"
     misc_feature    3814..3819
                     /label=PstI, BspMAI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CTGCA^G"
                     /note="/annotation_group=CTGCAG"
     misc_feature    3901..3906
                     /label=ApaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGGCC^C"
                     /note="/annotation_group=GGGCCC"
     misc_feature    3901..3906
                     /label=Bsp120I, PspOMI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GGCCC"
                     /note="/annotation_group=GGGCCC"
     misc_feature    4006..4011
                     /label=ApaLI, Alw44I, VneI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
                     /note="/annotation_group=GTGCAC"
     misc_feature    4018..4023
                     /label=ApaLI, Alw44I, VneI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
                     /note="/annotation_group=GTGCAC"
     misc_feature    4038..4043
                     /label=KroI, MroNI, NgoMIV
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^CCGGC"
                     /note="/annotation_group=GCCGGC"
     misc_feature    4101..4106
                     /label=PstI, BspMAI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CTGCA^G"
                     /note="/annotation_group=CTGCAG"
     misc_feature    4135..4140
                     /label=BsePI, BssHII, PauI, PteI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^CGCGC"
                     /note="/annotation_group=GCGCGC"
     misc_feature    4206..4211
                     /label=SphI, PaeI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
                     /note="/annotation_group=GCATGC"
     misc_feature    4208..4213
                     /label=EcoT22I, Mph1103I, NsiI, Zsp2I
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=ATGCA^T"
                     /note="/annotation_group=ATGCAT"
     misc_feature    4210..4215
                     /label=SphI, PaeI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
                     /note="/annotation_group=GCATGC"
     misc_feature    4239..4244
                     /label=SphI, PaeI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
                     /note="/annotation_group=GCATGC"
     misc_feature    4241..4246
                     /label=EcoT22I, Mph1103I, NsiI, Zsp2I
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=ATGCA^T"
                     /note="/annotation_group=ATGCAT"
     misc_feature    4243..4248
                     /label=SphI, PaeI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
                     /note="/annotation_group=GCATGC"
     misc_feature    4270..4275
                     /label=SphI, PaeI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
                     /note="/annotation_group=GCATGC"
     misc_feature    4272..4277
                     /label=EcoT22I, Mph1103I, NsiI, Zsp2I
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=ATGCA^T"
                     /note="/annotation_group=ATGCAT"
     misc_feature    4303..4308
                     /label=SphI, PaeI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
                     /note="/annotation_group=GCATGC"
     misc_feature    4305..4310
                     /label=EcoT22I, Mph1103I, NsiI, Zsp2I
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=ATGCA^T"
                     /note="/annotation_group=ATGCAT"
     misc_feature    4335..4340
                     /label=ApaLI, Alw44I, VneI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
                     /note="/annotation_group=GTGCAC"
     misc_feature    4370..4375
                     /label=SphI, PaeI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
                     /note="/annotation_group=GCATGC"
     misc_feature    4402..4407
                     /label=ApaLI, Alw44I, VneI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TGCAC"
                     /note="/annotation_group=GTGCAC"
     misc_feature    4425..4430
                     /label=PstI, BspMAI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CTGCA^G"
                     /note="/annotation_group=CTGCAG"
     misc_feature    4449..4454
                     /label=AgeI, AsiGI, BshTI, CspAI, PinAI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=A^CCGGT"
                     /note="/annotation_group=ACCGGT"
     misc_feature    4475..4480
                     /label=SphI, PaeI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
                     /note="/annotation_group=GCATGC"
     misc_feature    4493..4498
                     /label=PstI, BspMAI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CTGCA^G"
                     /note="/annotation_group=CTGCAG"
     misc_feature    4505..4510
                     /label=SphI, PaeI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCATG^C"
                     /note="/annotation_group=GCATGC"
     misc_feature    4555..4560
                     /label=Cfr9I, TspMI, XmaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^CCGGG"
                     /note="/annotation_group=CCCGGG"
     misc_feature    4713..4720
                     /label=PspXI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=VC^TCGAGB"
                     /note="/annotation_group=VCTCGAGB"
     misc_feature    4714..4719
                     /label=XhoI, PaeR7I, Sfr274I, SlaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^TCGAG"
                     /note="/annotation_group=CTCGAG"
     misc_feature    4834..4839
                     /label=BsePI, BssHII, PauI, PteI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^CGCGC"
                     /note="/annotation_group=GCGCGC"
     misc_feature    4836..4841
                     /label=BsePI, BssHII, PauI, PteI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^CGCGC"
                     /note="/annotation_group=GCGCGC"
     misc_feature    4914..4919
                     /label=BsePI, BssHII, PauI, PteI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^CGCGC"
                     /note="/annotation_group=GCGCGC"
     misc_feature    4977..4982
                     /label=BsePI, BssHII, PauI, PteI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^CGCGC"
                     /note="/annotation_group=GCGCGC"
     misc_feature    5000..5005
                     /label=BsePI, BssHII, PauI, PteI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^CGCGC"
                     /note="/annotation_group=GCGCGC"
     intron          5184..5993
                     /label=intron of chicken Beta-actin
                     /note="/Transferred_From=pCAG-mCherry-LFC"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/type=intron"
     misc_feature    5191..5196
                     /label=ApaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGGCC^C"
                     /note="/annotation_group=GGGCCC"
     misc_feature    5191..5196
                     /label=Bsp120I, PspOMI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GGCCC"
                     /note="/annotation_group=GGGCCC"
     misc_feature    5344..5349
                     /label=BseX3I, BstZI, EagI, EclXI, Eco52I
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^GGCCG"
                     /note="/annotation_group=CGGCCG"
     misc_feature    5647..5652
                     /label=KroI, MroNI, NgoMIV
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^CCGGC"
                     /note="/annotation_group=GCCGGC"
     misc_feature    5759..5764
                     /label=PluTI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGCGC^C"
                     /note="/annotation_group=GGCGCC"
     misc_feature    5759..5764
                     /label=KasI, SspDI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GCGCC"
                     /note="/annotation_group=GGCGCC"
     misc_feature    5803..5808
                     /label=PluTI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGCGC^C"
                     /note="/annotation_group=GGCGCC"
     misc_feature    5803..5808
                     /label=KasI, SspDI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GCGCC"
                     /note="/annotation_group=GGCGCC"
     misc_feature    5806..5811
                     /label=KroI, MroNI, NgoMIV
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^CCGGC"
                     /note="/annotation_group=GCCGGC"
     primer_bind     5934..5952
                     
     misc_feature    6001..6006
                     /label=NheI, AsuNHI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^CTAGC"
                     /note="/annotation_group=GCTAGC"
     misc_feature    6001..6006
                     /label=BmtI, BspOI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GCTAG^C"
                     /note="/annotation_group=GCTAGC"
     misc_feature    6006..6011
                     /label=XhoI, PaeR7I, Sfr274I, SlaI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=C^TCGAG"
                     /note="/annotation_group=CTCGAG"
     misc_feature    6011
                     /label=lastNtBeforeIntegration
                     /note="/created_by=schelskim"
     CDS             6012..6902
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     misc_feature    6903..6908
                     /label=HindIII
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=A^AGCTT"
                     /note="/annotation_group=AAGCTT"
     misc_feature    6903
                     /label=firstNtafterIntegration
                     /note="/created_by=schelskim"
     misc_feature    6910..6915
                     /label=EcoRI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^AATTC"
                     /note="/annotation_group=GAATTC"
     misc_feature    6915..6920
                     /label=PstI, BspMAI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CTGCA^G"
                     /note="/annotation_group=CTGCAG"
     misc_feature    join(6920..6923,1..2)
                     /label=SalI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^TCGAC"
                     /note="/annotation_group=GTCGAC"
ORIGIN
        1 acggtaccgc gggcccggga tccaccggat ctagataagt aatgatcata atcagccata
       61 tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      121 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      241 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaattcg taatcatgtc
      301 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      361 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      421 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      481 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      541 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      601 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      661 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      721 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      781 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      841 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      901 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      961 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1021 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1081 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1141 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1201 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1261 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1321 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1381 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1441 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1501 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1561 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1621 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1681 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1741 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1801 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1861 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1921 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1981 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2041 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2101 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2161 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2221 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2281 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2341 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2401 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2461 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     2521 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2581 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     2641 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     2701 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     2761 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     2821 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     2881 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ttgggatctt tgcattggcc
     2941 cacggctctc aggatgggga tgctcccctt cagcacccgg ttccccttgg aaactgatgg
     3001 tcctggctct gtggcatggc agtggcactg tgaggagccc cctaccagca gcacacagtg
     3061 gggttggcac tgccacgctc cggatgccgc gctctgatcc aaccccataa tcaagggaac
     3121 ccgaattgcc ccatcattgc ccccaccacc cccatcctgc cgggccctca caccccacgc
     3181 tgccttctgg tgacattccc cagcccaaac ccacggcttc atggctaccg cggggcattt
     3241 cccattgccg ccccattatc agctctgcac acctcccgct gtacccatgc ctcgtggctg
     3301 cccttctttg acgtataatc ttctaattaa tacccggcct tgtcaaagtg gagcacaaac
     3361 gttaattaat tccccagcag gcaggtaatt aacagtgtga ctcccttttt gctgcgagtg
     3421 gggctgatac agagagatgt ggcactatgg agcccacggg gtcctggcac tgggtgccca
     3481 cggaggtccc catgtgctgc agtgtcaccg cctccgagga gacagtattg tccctgcggt
     3541 gtccctgcag ctcagctctg tccacagggc cacctccagt ttggagggga cacaatgcag
     3601 ccccgatgca acccatcctc gcagcatccc agggacaaag accccactgc aagaccgcac
     3661 acagggctgg gtcccgctcc cctaatatct acagtgcttc ttgcatggcc ccttaatcaa
     3721 tgcagttaat cagcatgcgc tcatgcaccg ctctggagct gcaaagcccc tcgcagcgct
     3781 gctcaccaac accgcgcacc gccccggccc agcctgcagc acgcgctgca aacaggaaag
     3841 aaacaaaata ttgcccaaat gtaggcaaag gcattcggct gccttgacct ccgccgggcc
     3901 gggccctgcc tgactcagct ccttactcag cgctcgcttc ctccctccgg ctgccaccgc
     3961 cgcagcgcac accctgacaa agagtggccc ttaacgggct ctgaggtgca cccagcagtg
     4021 cactcagcag tccaagggcc ggcctggagg tttgcaccgc tacgtgctga cattagcatt
     4081 gaacttggcc ctgggtagtg ctgcaggccg ggcggggtgg gtgtagagag tgcagcgcgc
     4141 gttgcacccg gtgccccttc ccctcccttg catcccagca ggctgcaccc cagcaccagg
     4201 cccgtgcatg catgctcctg gtgttattgc agcctggtgc atgcatgcgt cttgatggtg
     4261 cagcgctgtg catgcatcct ccttggtgtg tagcagtcta gtgcatgcat acccctcggt
     4321 gttattgctg ctctgtgcac gcacgctcat tgtatcactt catcccagtg catgcactca
     4381 cactggagcg attgctgctc ggtgcacgca cactcattgt atcactgcag ctcagtggct
     4441 gcacgcacac cggtgttatt gctgctcggt gcgtgcatgc acatcagtgt cgctgcagct
     4501 cagtgcatgc acgctcattg cccatcgcta tccctgcctc tcctgctggc gctccccggg
     4561 aggtgacttc aaggggaccg caggaccacc tcgggggtgg ggggagggct gcacacgcgg
     4621 accccgctcc ccctccccaa caaagcactg tggaatcaaa aaggggggag gggggatgga
     4681 ggggcgcgtc acacccccgc cccacaccct cacctcgagg tgagccccac gttctgcttc
     4741 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     4801 ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg
     4861 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     4921 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     4981 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     5041 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     5101 cccttctcct ccgggctgta attagcgctt ggtttaatga cggctcgttt cttttctgtg
     5161 gctgcgtgaa agccttaaag ggctccggga gggccctttg tgcggggggg agcggctcgg
     5221 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
     5281 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
     5341 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
     5401 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcggcggtc gggctgtaac
     5461 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     5521 cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
     5581 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
     5641 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     5701 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     5761 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     5821 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctcca tctccagcct
     5881 cggggctgtc cgcaggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     5941 ttctggcgtg tgaccggcgg ggtttatatc ttcccttctc tgttcctccg cagcccccaa
     6001 gctagctcga gatggcagaa atcggtactg gctttccatt cgacccccat tatgtggaag
     6061 tcctgggcga gcgcatgcac tacgtcgatg ttggtccgcg cgatggcacc cctgtgctgt
     6121 tcctgcacgg taacccgacc tcctcctacg tgtggcgcaa catcatcccg catgttgcac
     6181 cgacccatcg ctgcattgct ccagacctga tcggtatggg caaatccgac aaaccagacc
     6241 tgggttattt cttcgacgac cacgtccgct tcatggatgc cttcatcgaa gccctgggtc
     6301 tggaagaggt cgtcctggtc attcacgact ggggctccgc tctgggtttc cactgggcca
     6361 agcgcaatcc agagcgcgtc aaaggtattg catttatgga gttcatccgc cctatcccga
     6421 cctgggacga atggccagaa tttgcccgcg agaccttcca ggccttccgc accaccgacg
     6481 tcggccgcaa gctgatcatc gatcagaacg tttttatcga gggtacgctg ccgatgggtg
     6541 tcgtccgccc gctgactgaa gtcgagatgg accattaccg cgagccgttc ctgaatcctg
     6601 ttgaccgcga gccactgtgg cgcttcccaa acgagctgcc aatcgccggt gagccagcga
     6661 acatcgtcgc gctggtcgaa gaatacatgg actggctgca ccagtcccct gtcccgaagc
     6721 tgctgttctg gggcacccca ggcgttctga tcccaccggc cgaagccgct cgcctggcca
     6781 aaagcctgcc taactgcaag gctgtggaca tcggcccggg tctgaatctg ctgcaagaag
     6841 acaacccgga cctgatcggc agcgagatcg cgcgctggct gtcgacgctc gagatttccg
     6901 gcaagcttcg aattctgcag tcg
//
