LOCUS       Exported               12477 bp ds-DNA     circular SYN 23-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12477)
  AUTHORS   Kazuhiko
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 12, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12477
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..19)
                     /direction=LEFT
                     /label=Deleated f1 ori
                     /note="f1 ori;f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis;Geneious name: 
                     f1 ori rep origin"
     misc_feature    30..180
                     /label=Tolmini-L
     promoter        188..206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        226..243
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(300..352)
                     /label=E1b
     misc_feature    368..384
                     /label=UAS
     misc_feature    387..403
                     /label=UAS
     misc_feature    406..422
                     /label=UAS
     misc_feature    425..441
                     /label=UAS
     misc_feature    444..460
                     /label=UAS
     misc_feature    473..568
                     /label=Spacer
     misc_feature    594..610
                     /label=UAS
     misc_feature    613..629
                     /label=UAS
     misc_feature    632..648
                     /label=UAS
     misc_feature    651..667
                     /label=UAS
     misc_feature    670..686
                     /label=UAS
     misc_feature    702..754
                     /label=E1b
     CDS             771..815
                     /codon_start=1
                     /product="Fyn kinase membrane targeting signal"
                     /label=Fyn
                     /translation="MGCVQCKDKEATKLT"
     CDS             822..1538
                     /codon_start=1
                     /label=zmClover3
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             1554..1607
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1608..1988
                     /codon_start=1
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAKD"
     CDS             2004..2723
                     /codon_start=1
                     /label=zmTurquoise2
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(2740..2757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2764..3185
                     /label=AFPpA
                     /note="3'UTR containing PolyA signal of the ocean pout 
                     antifreeze protein gene   "
     misc_feature    3244..3310
                     /label=custom polyadenylation/transcription stop signal
     3'UTR           complement(3318..4228)
                     /label=zf Tubulin beta 5 (Tubb5) 3´ UTR
                     /note=" zf Tubulin beta 5 (Tubb5)   3´ UTR  
                       The Journal of Neuroscience, October 2, 2013 • 
                     33(40):15726 –15734   Zebrafish Embryonic Neurons Transport
                     Messenger RNA to Axons and Growth Cones In Vivo   
                     
                     "
     CDS             complement(4287..5216)
                     /codon_start=1
                     /label=emiRFP670(Zebrafish)
                     /translation="MAEGSVARQPDLLTCEHEEIHLAGSIQPHGALLVVSEHDHRVIQA
                     SANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLM
                     HRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVM
                     VYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVTYQPVP
                     LEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHY
                     LPRFIRFELRAICKRLAERIATRITALES"
     CDS             complement(5217..6146)
                     /codon_start=1
                     /label=zf-synaptopysin2b
                     /translation="MDVANQLVATGQFTIVKQPLGFIKILQWFFAIFAFATCGGYSGVF
                     RMSVECENRSESDLNIEVEFAYPFRLHQVWFDAPTCKGSEPERLFLVGDNSSSAEFFVT
                     IGVFSFLYSMAAISVYIFLLEKYREGNKGAQADFIVTAVFTFMWLVSSSAWAKGLSDVK
                     RATDPDDVINLIPACEREENRCKELHEPVVSGLNTSVAFGFCNVILWGGNLWFIFKETG
                     WMAAFAGTYMPSGEKQPAPDSFGQGYGQEGYGQDQYASSQGGYQPDYGQQGGYEGGGYN
                     QGVYGQGEPTSFSNEMCGGGPGIHRPVAT"
     misc_feature    complement(6178..6230)
                     /label=E1b
     misc_feature    6246..6262
                     /label=UAS
     misc_feature    6265..6281
                     /label=UAS
     misc_feature    6284..6300
                     /label=UAS
     misc_feature    6303..6319
                     /label=UAS
     misc_feature    6322..6338
                     /label=UAS
     misc_feature    6367..6462
                     /label=Spacer
     misc_feature    6488..6504
                     /label=UAS
     misc_feature    6507..6523
                     /label=UAS
     misc_feature    6526..6542
                     /label=UAS
     misc_feature    6545..6561
                     /label=UAS
     misc_feature    6564..6580
                     /label=UAS
     misc_feature    6596..6648
                     /label=E1b
     CDS             6669..9071
                     /codon_start=1
                     /label=zf-PSD95
                     /translation="MPLKREDTERALQAMEACQSAGDEGFRTRAERLLTIFQSDLFQAL
                     LDIQEFYELTVFENQTAGRALTPGLKYRYHDEETPPLQHSPAHLSTGKSAEMLHLGDSG
                     HAPIDGIHAYTPQMHVSPAKPVLLPSGHAPYYATSTLMNGMDGDVEYEEITLERGNSGL
                     GFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNDVDVREVTHSFAV
                     EALKEAGPIVRLYVLRHKPSAEKITELKLIKGPKGLGFSIAGGVGNQHVPGDNSIYVTK
                     IIEGGAAHKDGRLQIGDKILAVNNMYLEEVMHEDAVAALKNTGDVVFLRVAKTLHQHHH
                     QDAYNPPDITSSYSPHMDMSDYPQALSPSSPRRYSPIPKGLFLDDDISREPRRVVIHRG
                     STGLGFNIVGGEDGEGIFISFILAGGAADLSGELRKGDQILSVNGVDLRHATHEQAAAA
                     LKNAGQTVTIITQYRPEEYSRFEAKIHDLREQLMNSSLVSAAASLRSGKRSFFIRALFD
                     YDKTADGGFLSQAVSFRFGDVLQVFDCSDEEWWQAGKLAPHGELEETGYIPSKRRVERK
                     EWSRLKTRGREPVSGRSDYIVSHETVTQSEVHYARPVIILGPSKDRVNDDLLSEFPDKF
                     GSCVPHTTRPKREYEMDGRDYHFVSSREQMEKDIQSHRFIEAGQYNSHLYGTSVQSVRQ
                     VAEQQGKHCILDVSANAVRRLQAAQLYPIAIFIRPSSLQNVLNISKRLTEEQARRALDR
                     AVKLEQDFIECFSAIVEGESFEEIYHHVKSVIEEQSGPYSWIPARERL"
     CDS             9108..9803
                     /codon_start=1
                     /label=zmScarlet-I
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     polyA_signal    9837..9971
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(10074..10092)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    10099..10299
                     /label=Tol2miniR
     primer_bind     complement(10328..10344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10352..10368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10376..10406)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(10730..11318)
                     /direction=LEFT
                     /label=ori
                     /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication;Geneious name: ori rep origin"
     CDS             complement(11489..12349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics;Geneious name: bla 
                     CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12350..12454)
                     /gene="bla"
                     /label=bla AmpR promoter
                     /note="AmpR promoter;Geneious name: bla promoter"
ORIGIN
        1 ctaaattgta agcgttaatc ccatatgggc agaggtgtaa aaagtactca aaaattttac
       61 tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg
      121 ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac ttgagtatta
      181 agatctgtaa tacgactcac tatagggaca tagaatgcag gtacgtaata cgactcacta
      241 tagttctagc ctcgagacta gtcctaggcc atcatcgata agcttgatat cagtttaacg
      301 tgtggaggag ctcaaagtga ggctgagacg cgatgggatc cattatatac cctctagagt
      361 ctccgctcgg aggacagtac tccgctcgga ggacagtact ccgctcggag gacagtactc
      421 cgctcggagg acagtactcc gctcggagga cagtactccg acctgcagcc cgtcctagct
      481 agggctgctc tccaatctct gtcatggcag ggccactggc atccctgaag ttggtggaga
      541 ccacctcact ccactcagca tacagctcat ccagtcctct tagggctgca ggtcggagta
      601 ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
      661 tcctccgagc ggagtactgt cctccgagcg gagactctag agggtatata atggatccca
      721 tcgcgtctca gcctcacttt gagctcctcc acacgaattc gatatccacc atgggctgtg
      781 tgcaatgtaa ggataaagaa gcaacaaaac tgacgtcgac gatggtgagc aagggcgagg
      841 agctgttcac aggagtggtg cctatcctgg tggagctgga cggagacgtg aacggacaca
      901 agttcagcgt gagaggagag ggagagggag acgctacaaa cggaaagctg acactgaagt
      961 tcatctgtac aacaggaaag ctgcctgtgc cttggcctac actggtgaca acattcggat
     1021 acggagtggc ttgtttcagc agataccctg accacatgaa gcagcacgac ttcttcaagt
     1081 ctgctatgcc tgagggatac gtgcaggaga gaacaatcag cttcaaggac gacggaacat
     1141 acaagacaag agctgaggtg aagttcgagg gagacacact ggtgaacaga atcgagctga
     1201 agggaatcga cttcaaggag gacggaaaca tcctgggaca caagctggag tacaacttca
     1261 acagccacta tgtgtacatc acagctgaca agcagaagaa ctgtatcaag gctaacttca
     1321 agatcagaca caacgtggag gacggaagcg tgcagctggc tgaccactac cagcagaaca
     1381 cacctatcgg agacggacct gtgctgctgc ctgacaacca ctacctgagc caccagagca
     1441 agctgtctaa agaccctaac gagaagagag accacatggt gctgctggag ttcgtgacag
     1501 ctgctggaat cacacatggc atggacgagc tgtacaagac tagtggatcc ggcgagggaa
     1561 gaggaagtct gctgacctgc ggagacgtgg aggagaaccc tggacctatg ccagagccag
     1621 cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga
     1681 aaggcggcaa gaagcgcaag cgcagccgca aggagagcta ttccatctat gtgtacaagg
     1741 ttctgaagca ggtccaccct gacaccggca tttcgtccaa ggctatgggc atcatgaact
     1801 cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca
     1861 acaagcgctc gaccatcacc tccagggaga tccagacggc cgtgcgcctg ctgctgcctg
     1921 gggagttggc caagcatgcc gtgtccgagg gtactaaggc catcaccaag tacaccagcg
     1981 ctaaggaccc accggtcgcc accatggtgt caaaaggaga ggaactgttt actggagtcg
     2041 tgcctatcct ggtggagctg gatggagatg tgaatggaca caagttctct gtgtctggag
     2101 agggagaggg agatgctaca tatggaaagc tgacactgaa gttcatctgt acaacaggaa
     2161 agctgcctgt gccttggcct acactggtga caacactgtc ttggggagtg cagtgttttg
     2221 ctagataccc tgaccacatg aagcagcatg acttcttcaa gtctgctatg cctgagggat
     2281 atgtgcagga gagaacaatc ttcttcaagg atgatggaaa ctacaagaca agagctgagg
     2341 tgaagtttga gggagacaca ctggtgaaca gaattgagct gaagggaatt gacttcaagg
     2401 aggatggaaa catcctggga cacaagctgg agtacaatta tttctctgac aatgtctaca
     2461 tcacagctga caagcagaag aatggaatca aggctaactt caagatcaga cacaacattg
     2521 aggatggagg agtgcagctg gctgaccact accagcagaa cacacctatt ggagatggac
     2581 ctgtgctgct gcctgacaac cactacctga gcacacagtc taagctgagc aaggacccta
     2641 atgagaagag agaccacatg gtgctgctgg agtttgtgac agctgctgga atcacactgg
     2701 gcatggacga gctgtacaag taaactcgag cctctagaac tatagtgagt cgtattacct
     2761 agtaattaag tctcagccac cgttaactga acatgtcaaa acctgtggag actgttgaga
     2821 tttgatgttc tgaaaagata aagcctataa ataaaatgtt gcccaaattt cctgcctgat
     2881 gtttttcttt gtctttgcta catggctttg ctgctcggat cggctcactc tgtgtatgcc
     2941 acgttcactt tgtactctcc ttctcacggt aggtttatta tttttagatg tgcagttagt
     3001 ttctgtgaaa taacacacca cacactgata ttgtctgtgc attgacttgg tgagtgcaca
     3061 ttgtttttga tcttgacata tttatatttg attgatcagg tgaactgtgt gaatctaaag
     3121 tgctccatac agatgttctg cattgaaaat attctcattt tattagtgga agtgagtgta
     3181 tgccagcgca ggtaccgggc ccaatgcatt ggcgccgcgg ccggccgcta ccacgcacca
     3241 ccatgaagag ttttacagaa acgactataa agatgttaaa ttcaaaacca tttgttttat
     3301 tccgcggccg gccgcccttt tttttttttt tttttttttt tttttttttt taacgaaatt
     3361 gttttcattt ttattttggt tttctccaga ggctcagttt ccttccacac agggacaatc
     3421 aaggaacatc aaaacttttg aaatatataa tcaacaatat aacacttaaa acgatgggct
     3481 cagaaataag acataacaga gtccatgaat taaaaattgc accaacaatc cattcaacat
     3541 gcttgtcttt ctatttgcct aatctaaaat acttcccttt cctgtattgc ccttgccatc
     3601 cattgattta tgtcctcctg tagacgttgg ttggcgtcca agtgaggcca agattcaata
     3661 accctgtcat tccatcagtc cttttccata accacttcca cgacatgcct atcttttcca
     3721 aacatttctt gcaatactgc aatcagaccc ctggaggtca cattgaagtg ccattatttc
     3781 acagaattgc cctgttccag tgcaatagta acaaaagaaa tcacacaaat aaaacacgac
     3841 aaatttcact gaagttactt gtagataaaa gaagcaaacg ttcaaataaa acttaaatga
     3901 gatgttacag tcctcccaat ttgtaacact gattctacct ggatgtttgc cacttttcgc
     3961 ttccctgttc tgtactgcac catcagaatg gtaaactaat acactcatac acactaaaaa
     4021 gagtgaactg taatcgtaaa cactgttacc cactctaatc catgtttaaa aaacattctt
     4081 tctgccttcc tctggtcatt tttggcccct ttttgtctag tgctaatttc tacttcatct
     4141 gattgagaca ctttcataaa agttttgcta catttactca aaacttaaac acactaccac
     4201 ctccccaaaa caccctttag tttccttcga attgttgttg ttgtaatacg actcactata
     4261 gttctagagg ctcgagacta gatttagctc tccagagctg tgattcttgt agcgattctc
     4321 tcagccagtc tcttgcagat agctctcagc tcgaatctga tgaatctagg caggtagtgg
     4381 tggcacacca ccagtcccca cagctttcct cccaccacca ggctcacagc cagtgtagct
     4441 ctcactccca tgtccttcag gaactgcagg tggcaagggc tcatgcttct caggaagcat
     4501 ccgctcatgt ccaggtctct tcctgtcaga gggctcagtc taggctccag aggcacaggc
     4561 tggtatgtca cgtccaccag cactctcact ctctgtctca cgtacagctg tctagccatc
     4621 tgaggcactg tgctgctagg gtatctgttt ccgaagtagc tctccagtcc aggcacgtgg
     4681 cactcgctga acaccagtcc gtgtccctgc tcgtcgaatc tatacaccat cactctgtcg
     4741 tatcctgtgc actgctggaa cagcagcact gtgtcgtcgc acagagctct caggcttcca
     4801 gctgttctga ttctctccag agcaggagcc agtgttccgc tcaggtcgat gctaggtcca
     4861 gctctctcca gctcgatgat cagtcctccc tcaggaggtc tgtgcatcag tccgcagtac
     4921 tctgtgctag ggtttccgat tctgcatctc acagccacag gcattccctc agctgtaggg
     4981 tccaggtgag gcaggatctt gatcagcagg tctccgtcga tctcagccag aggcactccc
     5041 agcacgcttc ccaggttcag gaactcagca gcgttagcag aagcctggat cactctgtgg
     5101 tcgtgctcgc tcaccaccag cagagctccg tgaggctgga tgcttccagc caggtggatc
     5161 tcctcgtgct cgcatgtcag caggtcaggc tgtctagcca cgcttccctc agccatggtg
     5221 gcgaccggcc ggtggatccc gggtcctcct ccacacatct cgttggagaa ggatgtgggc
     5281 tctccctgac catacactcc ttgattgtat ccaccgcctt catatccacc ctgctggccg
     5341 tagtcaggct gatatccacc ctgagagctg gcatactggt cctgtccata accttcctgt
     5401 ccatagccct ggccgaagga gtctggagct ggctgcttct ctccagatgg catgtaggtg
     5461 ccagcgaaag ccgccatcca gccggtttcc ttgaagatga accagaggtt gcctccccat
     5521 aggatcacat tacagaaccc gaatgccaca gatgtgttca ggccagacac caccggttcg
     5581 tgcagctctt tgcatcggtt ttcctctcgt tcgcacgcag ggatgaggtt gatgacgtca
     5641 tcagggtcgg tggctctctt cacgtctgag agacctttag cccacgccga tgaactcacc
     5701 agccacatga aggtgaaaac cgctgtcaca atgaaatcag cctgagctcc tttgtttccc
     5761 tcgcggtatt tctccaacag gaagatgtac acagaaatgg ctgccattga ataaaggaag
     5821 gagaagacgc cgatggtgac gaagaactct gctgaggaag agttgtctcc caccagaaag
     5881 agtcgctcgg gttctgatcc tttacacgta ggggcatcaa accatacctg gtgcagtctg
     5941 aacgggtacg caaactcaac ctcaatgttc aggtcactct ctgagcggtt ctcacactcc
     6001 acgctcatac gaaacactcc agagtaacca ccgcaggtgg cgaacgcgaa gatggcaaag
     6061 aaccattgta agattttgat gaatcctaat ggctgtttga cgattgtaaa ctgcccagtg
     6121 gcgaccaact ggttggcaac atccatggtg gcgatcgttt aaacagatcg tttaaacgtg
     6181 tggaggagct caaagtgagg ctgagacgcg atgggatcca ttatataccc tctagagtct
     6241 ccgctcggag gacagtactc cgctcggagg acagtactcc gctcggagga cagtactccg
     6301 ctcggaggac agtactccgc tcggaggaca gtactccgac ctgcagcccg ggggatcgtc
     6361 gacaggtcct agctagggct gctctccaat ctctgtcatg gcagggccac tggcatccct
     6421 gaagttggtg gagaccacct cactccactc agcatacagc tcatccagtc ctcttagggc
     6481 tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     6541 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact ctagagggta
     6601 tataatggat cccatcgcgt ctcagcctca ctttgagctc ctccacacga attcagatat
     6661 cagccatcat gcctctcaaa cgagaagata cggagcgtgc gctgcaggcc atggaagcct
     6721 gtcagtctgc aggtgatgag ggtttcagga ctcgcgcaga gagacttctg accatctttc
     6781 agagtgacct gtttcaggct ctgctggaca tccaggagtt ctatgagctg acggtgtttg
     6841 agaatcagac ggcaggtaga gctctcacac cgggactgaa gtaccgttac catgacgagg
     6901 agacgccgcc actgcagcac agtcccgctc acctgagcac aggtaaatct gcagagatgc
     6961 tccacctggg tgactctggc cacgccccca tcgacggcat ccacgcatac acacctcaga
     7021 tgcacgtgtc acctgcaaag ccagtgttac tcccgagtgg acacgctccc tactacgcaa
     7081 cctctactct gatgaacggt atggacggag atgttgaata tgaggagatc acgctggaga
     7141 gggggaactc tgggctgggc ttcagtatcg ccggagggac tgataaccct catataggag
     7201 acgatcccag tatcttcatc actaagatca taccaggagg agctgcggca caggacggac
     7261 gactcagagt gaacgacagc attctgttcg tcaatgacgt ggatgtgcgg gaagtcacac
     7321 acagctttgc ggtggaggcg ctgaaggaag caggaccgat cgtccggctg tacgtgctgc
     7381 gccacaaacc atcagcagag aaaatcactg agctcaagct catcaaaggc cctaaaggtc
     7441 tgggcttcag tattgcgggt ggtgtgggga accagcacgt gcccggagac aacagcatct
     7501 acgttaccaa gatcatcgag ggcggagctg cacataaaga cggacggctg cagatcggag
     7561 acaagatcct cgctgtgaac aacatgtatc tggaggaggt gatgcatgaa gacgctgtag
     7621 cggcgctgaa gaacactgga gacgtggtgt ttctgagagt cgccaaaaca ctccatcagc
     7681 atcatcatca ggacgcctac aaccctcctg acatcaccag ctcatattct cctcatatgg
     7741 acatgtctga ttacccgcaa gccctcagtc cctcgtctcc gcgccgttac tcccccatcc
     7801 ctaaaggcct gtttctggac gacgacattt ccagggagcc gcggcgtgtg gtgatccaca
     7861 gaggctctac aggtctgggc tttaatatcg tgggtggaga agatggagag ggaatcttca
     7921 tctccttcat actggctgga ggagctgctg atctgagcgg agagctgagg aaaggagacc
     7981 agatactgag tgtgaacggt gtagatctgc gtcacgccac tcatgaacag gccgcggcgg
     8041 cactgaagaa cgcaggacag acggtcacca tcatcacaca gtacagacct gaagaataca
     8101 gcaggttcga ggccaagatc catgacctgc gggagcagct gatgaacagc agtttagtgt
     8161 ctgcagcggc ttcattacgg agcggaaaga gaagcttctt catcagggct ctgtttgact
     8221 atgataagac ggcagacggt ggttttctgt cgcaggccgt gagcttcagg ttcggggatg
     8281 ttctgcaggt ctttgactgc agtgatgagg aatggtggca ggccggaaaa ctggctccac
     8341 acggagaact ggaggagacc gggtacattc ccagcaagag gagggtggag cggaaggaat
     8401 ggtctcgtct gaagactcga ggccgagagc ccgtcagcgg acgcagcgat tacatcgtga
     8461 gccatgagac ggtgacgcag agtgaagtgc actacgcgcg tcccgtcata atcctcggac
     8521 ccagcaagga ccgtgtgaac gatgacctcc tctccgagtt cccggacaag tttggctcct
     8581 gtgtgcccca cacgacgcgg ccgaagcgcg agtacgagat ggacggacgc gattaccact
     8641 ttgtgtcgtc tcgagagcag atggagaagg acattcagag ccatcgcttc atcgaggcgg
     8701 gacagtacaa cagtcacctg tacggcacca gcgtccagag cgtcagacag gtggcagagc
     8761 agcaagggaa gcactgtatc ctggatgttt cagcgaacgc agtgcggagg ctacaggccg
     8821 ctcagcttta tcccatcgcc atctttatac gaccctcatc actgcagaac gtactaaaca
     8881 ttagcaagcg tctgacggag gaacaggcca gaagagcgtt agacagagcc gtcaaactgg
     8941 agcaggactt catcgagtgt ttctcagcga tcgtggaggg cgagagcttc gaggagatct
     9001 accatcatgt gaagtctgtg atcgaggagc agtccggacc gtatagctgg atcccagcac
     9061 gagagagact gggaggagga cccgggatcc accggccggt cgccaccatg gtgagcaagg
     9121 gcgaggcagt gatcaaggag ttcatgagat tcaaggtgca catggaggga agcatgaacg
     9181 gccacgagtt cgagatcgag ggagagggag agggaagacc ttacgaggga acacagacag
     9241 ctaagctgaa ggtgacaaag ggaggaccac tgcctttctc ttgggacatc ctgagccctc
     9301 agttcatgta cggaagcaga gctttcatca agcaccctgc tgacatccct gactactaca
     9361 agcagagctt ccctgaggga ttcaagtggg agagagtgat gaacttcgag gacggaggag
     9421 ctgtgacagt gacacaggac acaagcctgg aggacggaac actgatctac aaggtgaagc
     9481 tgagaggaac aaacttccct cctgacggac ctgtgatgca gaagaagaca atgggatggg
     9541 aggcttctac agagagactg taccctgagg acggagtgct gaagggagac atcaagatgg
     9601 ctctgagact gaaggacgga ggaagatacc tggctgactt caagacaaca tacaaggcta
     9661 agaagcctgt gcagatgcct ggagcttaca acgtggacag aaagctggac atcacaagcc
     9721 acaacgagga ctacacagtg gtggagcagt acgagagaag cgagggaaga cacagcaccg
     9781 gcggcatgga cgagctgtac aagtaaatct agaactatag tgagtcgtat tacgtagatc
     9841 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     9901 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     9961 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
    10021 gggaggtttt ttaattcgcg gccgccaccg cggtggagct ccagcttttg ttccctttag
    10081 tgagggttaa ttgggcccaa gtgatctcca aaaaataagt actttttgac tgtaaataaa
    10141 attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta aaagtaaaag
    10201 tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata cttaagtaca
    10261 gtaatcaagt aaaattactc aagtacttta cacctctggg tactgtttcg cgcttggcgt
    10321 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    10381 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    10441 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    10501 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    10561 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    10621 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    10681 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    10741 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10801 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10861 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10921 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10981 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    11041 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    11101 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    11161 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    11221 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    11281 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    11341 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    11401 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    11461 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    11521 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    11581 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    11641 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    11701 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    11761 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    11821 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    11881 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    11941 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    12001 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    12061 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    12121 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    12181 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    12241 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    12301 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    12361 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    12421 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
