LOCUS       Exported                5941 bp ds-DNA     circular UNA 11-JAN-2023
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 12, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /label=Geneious name: source unspecified
     source          30..232
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /label=Geneious name: source unspecified
     source          1889
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /label=Geneious name: source unspecified
     rep_origin      complement(3..19)
                     /direction=LEFT
                     /label=Deleated f1 ori
                     /note="f1 ori;f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis;Geneious name: 
                     f1 ori rep origin"
     misc_feature    30..180
                     /label=Tolmini-L
     promoter        188..206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        234..533
                     /label=NBT promoter
     misc_feature    550..570
                     /label=NRSE
     misc_feature    588..608
                     /label=NRSE
     CDS             626..1888
                     /codon_start=1
                     /product="NFB=NFkB p65 subunit"
                     /label=KalNFB
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSSRSRPMEFQYLPDTDD
                     RHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTS
                     SLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPV
                     LAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDN
                     SEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSG
                     DEDFSSIADMDFSALLSQISS"
     3'UTR           1943..2019
                     /label=3xmiR1-T
                     /label=3xIPT miR-1
                     /note="3xmiRNA1 target"
     misc_feature    2054..3241
                     /label=3'UTR syt2a
     misc_feature    3242..3469
                     /label=SV40 polyA
     promoter        complement(3538..3556)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    3563..3763
                     /label=Tol2miniR
     primer_bind     complement(3792..3808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3816..3832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3840..3870)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(4194..4782)
                     /direction=LEFT
                     /label=ori
                     /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication;Geneious name: ori rep origin"
     CDS             complement(4953..5813)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics;Geneious name: bla 
                     CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5814..5918)
                     /gene="bla"
                     /label=bla AmpR promoter
                     /note="AmpR promoter;Geneious name: bla promoter"
ORIGIN
        1 ctaaattgta agcgttaatc ccatatgggc agaggtgtaa aaagtactca aaaattttac
       61 tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg
      121 ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac ttgagtatta
      181 gatcctgtaa tacgactcac tataggttaa ttaagacaaa gaatgcaggt acacgagcag
      241 catccatttc cattccctcc cttgtcattt gattggttcc catccctaca atgctcaggg
      301 ccagtggctc cacccaccat tacatcatct gagcccagga ccacagcaag tgaataggag
      361 agagcagcac cctccccaag gcgtcacaag tgtataaaag gcagcagaag gagcctctga
      421 cagcccagat tcttagccgg ctggaaacca tcggggtgtt gcacaaaggg caagtaaagc
      481 aacaagaaag ggccacacca tccactgatt acaccactgt aagactcaac ccaatcaagc
      541 ttggatccct tcagcaccaa ggacagggcc tctaggacac tagaggtttc agcaccaagg
      601 acagggcctc gaattcgccg ccaccatgaa actgctctca tccatcgagc aagcctgcga
      661 catttgtcgg cttaagaagc tgaaatgctc caaggaaaag ccgaaatgtg ccaaatgcct
      721 gaagaacaat tgggaatgtc gttactctcc caaaaccaag cgaagtccac tcacaagggc
      781 tcatctgacc gaagtggaga gcaggctaga gagactggaa caactctttt tgctcatctt
      841 ccctagagag gaccttgaca tgatcctcaa gatggattct ctccaggata ttaaagccct
      901 tttgactggc ttattcgtcc aggacaatgt gaacaaagac gctgtgacag accgattggc
      961 aagtgtcgag accgatatgc ctctgacact gagacagcac agaatcagcg ctacttcctc
     1021 aagcgaagag tcttctaaca agggacagag acagctgact gtttcgagca ggtcgaggcc
     1081 catggagttc cagtacctgc cagatacaga cgatcgtcac cggattgagg agaaacgtaa
     1141 aaggacatat gagaccttca agagcatcat gaagaagagt cctttcagcg gacccaccga
     1201 cccccggcct ccacctcgac gcattgctgt gccttcccgc agctcagctt ctgtccccaa
     1261 gccagcaccc cagccctatc cctttacgtc atccctgagc accatcaact atgatgagtt
     1321 tcccaccatg gtgtttcctt ctgggcagat cagccaggcc tcggccttgg ccccggcccc
     1381 tccccaagtc ctgccccagg ctccagcccc tgcccctgct ccagccatgg tatcagctct
     1441 ggcccaggcc ccagcccctg tcccagtcct agccccaggc cctcctcagg ctgtggcccc
     1501 acctgccccc aagcccaccc aggctgggga aggaacgctg tcagaggccc tgctgcagct
     1561 gcagtttgat gatgaagacc tgggggcctt gcttggcaac agcacagacc cagctgtgtt
     1621 cacagacctg gcatccgtcg acaactccga gtttcagcag ctgctgaacc agggcatacc
     1681 tgtggccccc cacacaactg agcccatgct gatggagtac cctgaggcta taactcgcct
     1741 agtgacaggg gcccagaggc cccccgaccc agctcctgct ccactggggg ccccggggct
     1801 ccccaatggc ctcctttcag gagatgaaga cttctcctcc attgcggaca tggacttctc
     1861 agccctgctg agtcagatca gctcctaatc tagccctagg tgtacaggcg cgccactagt
     1921 tctacctctc gagaatctag aatacatact tctaatcatt ccatagctaa tacatacttc
     1981 taatcattcc atagctaata catacttcta atcattccat actagaacta tagtgagtcg
     2041 tagatctcgc gccaacccgc tcctcctccc tccctccctc cctccctcct ctcctttatt
     2101 cacttttcaa catgcacaac acgtcatcaa atcccccaat cagcactgaa ttacagccac
     2161 gggccaccag actgaaggtg ttaaagaaga cgattcggtc tcatcagcgg ctaaaacgag
     2221 atgagggtga accgagagag actgtttcca cagatgttct tgaaggaggg ccgggctcgt
     2281 aggtgcggtt attacacata taagctccca ttcacacctt aaaacatcca acagcattcc
     2341 aaagttttcc atcggggacc agggatagaa aacactgcct cttccactgc ctaatttaac
     2401 acattccttc tagattatct ctggtcatct agatgaaatc agttctgttc tgtttgccgt
     2461 gtctgaaaag acagtaaaga gtaattgaaa aaaaaaatga atatagaaaa atggggactg
     2521 gagagatatt tggactagac gctcctgatc cagccagaag gaacctttca ggtgtcgatt
     2581 acactgcctg cgaaaacaac ctcttcttcc attcttcagt ttccagctgc cggctggctt
     2641 cttccactgc ccaacatcct aacaaacccc tgacaaaaaa ggagacaagc cttgaaacat
     2701 cctgaaataa gcaattctta gctgccaggg acgtcttcct gttagcatca gtgtatttgt
     2761 ttcttttgct ttgatttgtt ttcttttttt attttatttt catggtaaat gtattctgcc
     2821 tgagcacttt aaacgaaggt gtttttgttc cagtcgatgc ttcgttttga acctgctttt
     2881 atatttatta acttttgttg tgtattttta atgtttaatc tttaaaggaa tagttcacct
     2941 acacacacac acacacacac acacacacac acaaatgaaa tgaagtcatc atttactcac
     3001 ttttctttct tttagctgtg gagcacaaaa cgagatgttt tgaagaatgt tcacgctgtt
     3061 gtttttcatg caatttaagt aaacgaagat caagggatgt tgagctatac aaatattcat
     3121 tatatgggca gcacggtcgc tggttcgagt cccggctggg ccagttggca tttctgagtt
     3181 tgcatattct ccctgtgttg gcatgggttt cctcctggtg ctccgtttct cccacagtcc
     3241 actagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     3301 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     3361 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3421 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatca ggccttggta
     3481 ccgtttaaac ttaaaatgca tgcggccgcc accgcggtgg agctccagct tttgttccct
     3541 ttagtgaggg ttaattgggc ccaagtgatc tccaaaaaat aagtactttt tgactgtaaa
     3601 taaaattgta aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta
     3661 aaagtattga tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag
     3721 tacagtaatc aagtaaaatt actcaagtac tttacacctc tgggtactgt ttcgcgcttg
     3781 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3841 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3901 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     3961 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4021 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4081 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4141 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4201 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4261 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4321 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4381 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4441 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4501 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4561 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4621 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4681 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4741 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4801 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4861 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4921 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4981 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5041 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5101 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5161 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5221 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5281 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5341 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5401 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5461 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5521 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5581 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5641 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5701 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5761 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5821 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5881 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5941 c
//
