LOCUS       Exported                5785 bp ds-DNA     circular SYN 11-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Kazuhiko
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 12, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /label=Geneious name: source unspecified
     source          30..223
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /label=Geneious name: source unspecified
     source          1880
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /label=Geneious name: source unspecified
     rep_origin      complement(3..19)
                     /direction=LEFT
                     /label=Deleated f1 ori
                     /note="f1 ori;f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis;Geneious name: 
                     f1 ori rep origin"
     misc_feature    30..180
                     /label=Tolmini-L
     promoter        187..205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        225..524
                     /label=NBT promoter
     misc_feature    541..561
                     /label=NRSE
     misc_feature    579..599
                     /label=NRSE
     CDS             617..1879
                     /codon_start=1
                     /product="NFB=NFkB p65 subunit"
                     /label=KalNFB
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSSRSRPMEFQYLPDTDD
                     RHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTS
                     SLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPV
                     LAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDN
                     SEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSG
                     DEDFSSIADMDFSALLSQISS"
     misc_feature    1898..3085
                     /label=3'UTR syt2a
     misc_feature    3086..3313
                     /label=SV40 polyA
     promoter        complement(3382..3400)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    3407..3607
                     /label=Tol2miniR
     primer_bind     complement(3636..3652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3660..3676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3684..3714)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(4038..4626)
                     /direction=LEFT
                     /label=ori
                     /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication;Geneious name: ori rep origin"
     CDS             complement(4797..5657)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics;Geneious name: bla 
                     CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5658..5762)
                     /gene="bla"
                     /label=bla AmpR promoter
                     /note="AmpR promoter;Geneious name: bla promoter"
ORIGIN
        1 ctaaattgta agcgttaatc ccatatgggc agaggtgtaa aaagtactca aaaattttac
       61 tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg
      121 ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac ttgagtatta
      181 gatctgtaat acgactcact atagggacat agaatgcagg tacacgagca gcatccattt
      241 ccattccctc ccttgtcatt tgattggttc ccatccctac aatgctcagg gccagtggct
      301 ccacccacca ttacatcatc tgagcccagg accacagcaa gtgaatagga gagagcagca
      361 ccctccccaa ggcgtcacaa gtgtataaaa ggcagcagaa ggagcctctg acagcccaga
      421 ttcttagccg gctggaaacc atcggggtgt tgcacaaagg gcaagtaaag caacaagaaa
      481 gggccacacc atccactgat tacaccactg taagactcaa cccaatcaag cttggatccc
      541 ttcagcacca aggacagggc ctctaggaca ctagaggttt cagcaccaag gacagggcct
      601 cgaattcgcc gccaccatga aactgctctc atccatcgag caagcctgcg acatttgtcg
      661 gcttaagaag ctgaaatgct ccaaggaaaa gccgaaatgt gccaaatgcc tgaagaacaa
      721 ttgggaatgt cgttactctc ccaaaaccaa gcgaagtcca ctcacaaggg ctcatctgac
      781 cgaagtggag agcaggctag agagactgga acaactcttt ttgctcatct tccctagaga
      841 ggaccttgac atgatcctca agatggattc tctccaggat attaaagccc ttttgactgg
      901 cttattcgtc caggacaatg tgaacaaaga cgctgtgaca gaccgattgg caagtgtcga
      961 gaccgatatg cctctgacac tgagacagca cagaatcagc gctacttcct caagcgaaga
     1021 gtcttctaac aagggacaga gacagctgac tgtttcgagc aggtcgaggc ccatggagtt
     1081 ccagtacctg ccagatacag acgatcgtca ccggattgag gagaaacgta aaaggacata
     1141 tgagaccttc aagagcatca tgaagaagag tcctttcagc ggacccaccg acccccggcc
     1201 tccacctcga cgcattgctg tgccttcccg cagctcagct tctgtcccca agccagcacc
     1261 ccagccctat ccctttacgt catccctgag caccatcaac tatgatgagt ttcccaccat
     1321 ggtgtttcct tctgggcaga tcagccaggc ctcggccttg gccccggccc ctccccaagt
     1381 cctgccccag gctccagccc ctgcccctgc tccagccatg gtatcagctc tggcccaggc
     1441 cccagcccct gtcccagtcc tagccccagg ccctcctcag gctgtggccc cacctgcccc
     1501 caagcccacc caggctgggg aaggaacgct gtcagaggcc ctgctgcagc tgcagtttga
     1561 tgatgaagac ctgggggcct tgcttggcaa cagcacagac ccagctgtgt tcacagacct
     1621 ggcatccgtc gacaactccg agtttcagca gctgctgaac cagggcatac ctgtggcccc
     1681 ccacacaact gagcccatgc tgatggagta ccctgaggct ataactcgcc tagtgacagg
     1741 ggcccagagg ccccccgacc cagctcctgc tccactgggg gccccggggc tccccaatgg
     1801 cctcctttca ggagatgaag acttctcctc cattgcggac atggacttct cagccctgct
     1861 gagtcagatc agctcctaat ctagttctag acgcgccaac ccgctcctcc tccctccctc
     1921 cctccctccc tcctctcctt tattcacttt tcaacatgca caacacgtca tcaaatcccc
     1981 caatcagcac tgaattacag ccacgggcca ccagactgaa ggtgttaaag aagacgattc
     2041 ggtctcatca gcggctaaaa cgagatgagg gtgaaccgag agagactgtt tccacagatg
     2101 ttcttgaagg agggccgggc tcgtaggtgc ggttattaca catataagct cccattcaca
     2161 ccttaaaaca tccaacagca ttccaaagtt ttccatcggg gaccagggat agaaaacact
     2221 gcctcttcca ctgcctaatt taacacattc cttctagatt atctctggtc atctagatga
     2281 aatcagttct gttctgtttg ccgtgtctga aaagacagta aagagtaatt gaaaaaaaaa
     2341 atgaatatag aaaaatgggg actggagaga tatttggact agacgctcct gatccagcca
     2401 gaaggaacct ttcaggtgtc gattacactg cctgcgaaaa caacctcttc ttccattctt
     2461 cagtttccag ctgccggctg gcttcttcca ctgcccaaca tcctaacaaa cccctgacaa
     2521 aaaaggagac aagccttgaa acatcctgaa ataagcaatt cttagctgcc agggacgtct
     2581 tcctgttagc atcagtgtat ttgtttcttt tgctttgatt tgttttcttt ttttatttta
     2641 ttttcatggt aaatgtattc tgcctgagca ctttaaacga aggtgttttt gttccagtcg
     2701 atgcttcgtt ttgaacctgc ttttatattt attaactttt gttgtgtatt tttaatgttt
     2761 aatctttaaa ggaatagttc acctacacac acacacacac acacacacac acacacaaat
     2821 gaaatgaagt catcatttac tcacttttct ttcttttagc tgtggagcac aaaacgagat
     2881 gttttgaaga atgttcacgc tgttgttttt catgcaattt aagtaaacga agatcaaggg
     2941 atgttgagct atacaaatat tcattatatg ggcagcacgg tcgctggttc gagtcccggc
     3001 tgggccagtt ggcatttctg agtttgcata ttctccctgt gttggcatgg gtttcctcct
     3061 ggtgctccgt ttctcccaca gtccactaga tcataatcag ccataccaca tttgtagagg
     3121 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     3181 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3241 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3301 tcatcaatgt atcaggcctt ggtaccgttt aaacttaaaa tgcatgcggc cgccaccgcg
     3361 gtggagctcc agcttttgtt ccctttagtg agggttaatt gggcccaagt gatctccaaa
     3421 aaataagtac tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta
     3481 aaaaaatgta attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa
     3541 aaatccccaa aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca
     3601 cctctgggta ctgtttcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     3661 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3721 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     3781 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3841 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3901 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3961 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4021 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4081 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4141 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4201 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4261 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4321 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4381 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4441 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     4501 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4561 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4621 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4681 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4741 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4801 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4861 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4921 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4981 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5041 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5101 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5161 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5221 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5281 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5341 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5401 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5461 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5521 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5581 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5641 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5701 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5761 cgcacatttc cccgaaaagt gccac
//
