LOCUS       pPD47                  10164 bp ds-DNA     circular     13-JAN-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pIM88.xdna.ape
            Ligation of : 88_pSF4 Tet CMV+intron Renilla+STOP
            12xPP7SL_1xfirefly siRNA sites+xrRNA12_24xMS2SL SV40 CTE+polyA.xdna
            [10139  nt] : (SalI[5330] / SalI[5330]) to 88_pSF4 Tet CMV+intron
            Renilla+STOP 12xPP7SL_1xfirefly siRNA sites+xrRNA12_24xMS2SL SV40
            CTE+polyA.xdna [794  nt] : (SalI[5330] / XhoI[6128])
            ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     primer_bind     complement(8..28)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(12..28)
                     /label="M13r"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(26..45)
                     /label="LacOp-for"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_binding    complement(34..56)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(60..79)
                     /label="pUCM13-rev-157"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(60..90)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     Promoter        complement(61..90)
                     /label="lac"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     rep_origin      complement(396..1078)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    491..496
                     /label="TATA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(1176..1835)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(1223..1242)
                     /label="AmpStop"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(1347..1352)
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_feature    1969..1988
                     /label="AmpStart"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     Promoter        complement(2075..2103)
                     /label="Amp prom"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     misc_feature    complement(2116..2121)
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_feature    2200..2205
                     /label="TATA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2213..2231
                     /label="QE-for"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(2263..2281)
                     /label="GEX3-rev-25"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(2291..2310)
                     /label="GEX3-rev"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             complement(2473..2541)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2561..2580
                     /label="pUCM13-52"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2593..2609
                     /label="M13-40"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2612..2628
                     /label="M13"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     2612..2629
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3153..3158)
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_feature    3233..3238
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_feature    3423..3428
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_feature    complement(3591..3596)
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_feature    3601..3606
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_recomb     complement(3716..3749)
                     /label="FRT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3716..3763
                     /label="FRT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3804..4200
                     /label="Tet-CMV"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    3809..3827
                     /label="TetO"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3845..3863
                     /label="TetO"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3881..3899
                     /label="TetO"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3917..3935
                     /label="TetO"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3953..3971
                     /label="TetO"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3989..4007
                     /label="TetO"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4025..4043
                     /label="TetO"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4077..4082
                     /label="TATA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4108..4137
                     /label="TSS-pIM52"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4111..4130
                     /label="SL-GC81"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4111..4130
                     /label="SL-GC81"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4207..4342
                     /label="intron"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
     misc_feature    4415..5350
                     /label="Renilla"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(4418..4462)
                     /label="Renilla_50mer_01"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4465..4509)
                     /label="Renilla_50mer_02"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(4512..4556)
                     /label="Renilla_50mer_03"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(4606..4650)
                     /label="Renilla_50mer_05"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(4653..4697)
                     /label="Renilla_50mer_06"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(4700..4744)
                     /label="Renilla_50mer_07"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(4778..4822)
                     /label="Renilla_50mer_08"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(4825..4869)
                     /label="Renilla_50mer_09"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(4872..4916)
                     /label="Renilla_50mer_10"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(4919..4963)
                     /label="Renilla_50mer_11"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(4966..5010)
                     /label="Renilla_50mer_12"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(5013..5057)
                     /label="Renilla_50mer_13"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(5060..5104)
                     /label="Renilla_50mer_14"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(5107..5151)
                     /label="Renilla_50mer_15"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(5154..5198)
                     /label="Renilla_50mer_16"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(5201..5245)
                     /label="Renilla_50mer_17"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5248..5292)
                     /label="Renilla_50mer_18"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(5295..5339)
                     /label="Renilla_50mer_19"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    5357..5749
                     /label="PP7_v2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     None            5373..5397
                     /label="PP7 stemloop 1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     None            5438..5464
                     /label="PP7 stemloop 2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5756..6148
                     /label="6xPP7ORF stem loops"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    5756..6148
                     /label="PP7_v2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     None            5772..5796
                     /label="PP7 stemloop 1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     None            5837..5863
                     /label="PP7 stemloop 2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    6191..6211
                     /label="firefly siRNA site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6191..6211
                     /label="Firefly siRNA site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    6248..6325
                     /label="xrRNA1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6402..6481
                     /label="xrRNA2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6482..7764
                     /label="MS2v4"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    6488..7753
                     /label="24xMS2v4 stem loops"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    7783..7955
                     /label="CTE"
                     /ApEinfo_revcolor="#802272"
                     /ApEinfo_fwdcolor="#802272"
     misc_feature    7961..8183
                     /label="polyA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    7965..8183
                     /label="Poly A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(8033..8054)
                     /label="pCI-rev"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    8074..8091
                     /label="EGFP-C-Rev"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(8080..8085)
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_feature    8110..8131
                     /label="FastBac-rev"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    8124..8141
                     /label="EGFP-C1-anti"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    8166..8185
                     /label="SV40-pA-rev"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    8199..8246
                     /label="F3"
                     /ApEinfo_revcolor="#c036ac"
                     /ApEinfo_fwdcolor="#c036ac"
     misc_feature    complement(8285..8301)
                     /label="pBABE-5-fwd"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    9263..9268
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_feature    9339..9344
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_feature    complement(9781..9786)
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_feature    9942..9947
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_feature    complement(9946..9951)
                     /label="TATA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(10006..10011)
                     /label="polyA consensus"
                     /ApEinfo_revcolor="#c0505a"
                     /ApEinfo_fwdcolor="#c0505a"
     misc_feature    complement(10109..10114)
                     /label="TATA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtcatcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2281 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2341 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2401 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2461 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2581 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattaattcc
     2641 aagctaagtg aaagactctg tctcaaaaac caacctgaca cctgagccgg agcgttggct
     2701 ctcactacca tagaatattt tttcacaaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca cacagtcata cacatagaca cagtcacaca cacacacatg cacacacaca
     2821 cacacacact cacacacaca catacataca cacattcaca cacacccata cacacatttt
     2881 cacagtcaca cacacaaaca caatcacaca cacacacatc aatcaatcaa tcaatcaata
     2941 taccaaaaat gatagctcat acctgtaatc ttagatgtgg gaaggctaaa acagcaggct
     3001 acccatgact tcaaggtcac cctgggttac agagtagcta atagtctagc ttgaactata
     3061 gtgtaggatc tttaaaaaaa aattaaccaa ccaaccaaca aacaaacaaa caaacaacaa
     3121 caacaacaaa tggaagacag aggacaaaaa aaaataaaag aaaaagtaga aagccaatgg
     3181 agatactatc ttaaaattag aaatatttat gacacattct gaatactcca tgtttatttc
     3241 aaggaagcca atgtaacttt aagtactatg gccatttaat gtaaatactt aagaaaaaaa
     3301 accaaattaa ttttgataca tgctgcatgt gtgaaagacc cccgctgacg ggtagtcaat
     3361 cactcagagg agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag
     3421 ggtttattgg atacacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag
     3481 tgaggggttg tgggctcgag ctcggggagc agaagcgcgc gaacagaagt cgagctagct
     3541 tgccaaacct acaggtgggg tctttcattc cccccttttt ctggagacta aataaaatct
     3601 tttattttat ctatggctcg tactctatag gcttcagctg gtgatattgt tgagtcaaaa
     3661 ctagagcctg gaccactgat atcctgtctt taacaaattg gactaatccg gatctgaagt
     3721 tcctatactt tctagagaat aggaacttcg gaataggaac ttcgcggcag atcttttgcg
     3781 gccgctttgg gccggccgaa ttctttactc cctatcagtg atagagaacg tatgaagagt
     3841 ttactcccta tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa
     3901 cgtataagga gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc
     3961 agtgatagag aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt
     4021 ttactcccta tcagtgatag agaacgtata agctttaggc gtgtacggtg ggcgcctata
     4081 aaagcagagc tcgtttagtg aaccgtcaga TGCGCCGGTG CAGCCGGCGC AGcgcctgga
     4141 gcaattccac aacacttttg tcttatacca actttccgta ccacttccta ccctcgtaaa
     4201 AccggTcagg taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc
     4261 ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc
     4321 cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag
     4381 agtacttaat acgactcact ataggctagc caccatggct tccaaggtgt acgaccccga
     4441 gcaacgcaaa cgcatgatca ctgggcctca gtggtgggct cgctgcaagc aaatgaacgt
     4501 gctggactcc ttcatcaact actatgattc cgagaagcac gccgagaacg ccgtgatttt
     4561 tctgcatggt aacgctgcct ccagctacct gtggaggcac gtcgtgcctc acatcgagcc
     4621 cgtggctaga tgcatcatcc ctgatctgat cggaatgggt aagtccggca agagcgggaa
     4681 tggctcatat cgcctcctgg atcactacaa gtacctcacc gcttggttcg agctgctgaa
     4741 ccttccaaag aaaatcatct ttgtgggcca cgactggggg gcttgtctgg cctttcacta
     4801 ctcctacgag caccaagaca agatcaaggc catcgtccat gctgagagtg tcgtggacgt
     4861 gatcgagtcc tgggacgagt ggcctgacat cgaggaggat atcgccctga tcaagagcga
     4921 agagggcgag aaaatggtgc ttgagaataa cttcttcgtc gagaccatgc tcccaagcaa
     4981 gatcatgcgg aaactggagc ctgaggagtt cgctgcctac ctggagccat tcaaggagaa
     5041 gggcgaggtt agacggccta ccctctcctg gcctcgcgag atccctctcg ttaagggagg
     5101 caagcccgac gtcgtccaga ttgtccgcaa ctacaacgcc taccttcggg ccagcgacga
     5161 tctgcctaag atgttcatcg agtccgaccc tgggttcttt tccaacgcta ttgtcgaggg
     5221 agctaagaag ttccctaaca ccgagttcgt gaaggtgaag ggcctccact tcagccagga
     5281 ggacgctcca gatgaaatgg gtaagtacat caagagcttc gtggagcgcg tgctgaagaa
     5341 cgagcagtaa gtcgacAGAT CCTACCTGCA AAGCACCAGA GGATATGGCC TCGGTGCCTC
     5401 AGCATCTTCA GATGTGCTGT CACTGTCCAA TCTTCAAGCC AGCAGAGCAT ATGGGCTCGC
     5461 TGGCTCCACT GTCATTACAG TGGACCTGAT CTTCTCCAAT CAAAGGAGCA GAGGATATGG
     5521 CCTCGCTCCC TCCGACTACA TCAGGCTGGA AGTGACATCC ATCATTCATC CCAGCAGAGC
     5581 ATATGGGCTC GCTGGGACCA CAGCATGCCT CGAAGTGCTG AGAGACTATC ATCAAACCGA
     5641 CAGAGGATAT GGCCTCGTCG GCTCCTACTC CCTCCTGGTG GAATACTTCT CCACTCTTCA
     5701 ACCCAGCAGA GCATATGGGC TCGCTGGGTC CATCAGAGAT ACAAGATCTc tcgacAGATC
     5761 CTACCTGCAA AGCACCAGAG GATATGGCCT CGGTGCCTCA GCATCTTCAG ATGTGCTGTC
     5821 ACTGTCCAAT CTTCAAGCCA GCAGAGCATA TGGGCTCGCT GGCTCCACTG TCATTACAGT
     5881 GGACCTGATC TTCTCCAATC AAAGGAGCAG AGGATATGGC CTCGCTCCCT CCGACTACAT
     5941 CAGGCTGGAA GTGACATCCA TCATTCATCC CAGCAGAGCA TATGGGCTCG CTGGGACCAC
     6001 AGCATGCCTC GAAGTGCTGA GAGACTATCA TCAAACCGAC AGAGGATATG GCCTCGTCGG
     6061 CTCCTACTCC CTCCTGGTGG AATACTTCTC CACTCTTCAA CCCAGCAGAG CATATGGGCT
     6121 CGCTGGGTCC ATCAGAGATA CAAGATCTct cgagCCGCGG tgcgtaagtc tgttaacaga
     6181 atgctgcata Cgctgagtac ttcgaaatgt catcctctgt ctgacactga acgtaatcca
     6241 gACGCGTaag tcaggccgga aaattcccgc caccggaagt tgagtagacg gtgctgcctg
     6301 cgactcaacc ccaggaggac tgggtgaaca aagctgcgaa gtgatccatg taagccctca
     6361 gaaccgtctc ggaaagagga ccccacatgt tgtagcttca aggcccaatg tcagaccacg
     6421 ccatggcgtg ccactctgcg gagagtgcag tctgcgacag tgccccagga ggactgggtg
     6481 aGGATCCTAC CTACAAACGG CACGAGCATC AGCCGTGCCG CCCAGCATCT TCATCACTGT
     6541 CCAATCTTCA AGCGTGCACG ATCACGGCAC GCTCCACTGT CATTACAGTC TTATCCAATC
     6601 AAACTCCAGC AGCATCAGCG CTGGAGCTCC GACTACATCA GGACATCCAT CATTCAACGC
     6661 TCGACGATCA CGCGAGCGTC CACAGCATGC CTCAGACACT ATCATCAAAC GGCTGCACGA
     6721 TCACGGCAGC CGCTCCTACT GCCTCTACGT TATCGACTCT TCTGCGACGA GCATCAGCCG
     6781 TCGCAACATC GGACATACCA GATCCTACCT ACAAACGGCA CGAGCATCAG CCGTGCCGCC
     6841 CAGCATCTTC ATCACTGTCC AATCTTCAAG CGTGCACGAT CACGGCACGC TCCACTGTCA
     6901 TTACAGTCTT ATCCAATCAA ACTCCAGCAG CATCAGCGCT GGAGCTCCGA CTACATCAGG
     6961 ACATCCATCA TTCAACGCTC GACGATCACG CGAGCGTCCA CAGCATGCCT CAGACACTAT
     7021 CATCAAACGG CTGCACGATC ACGGCAGCCG CTCCTACTGC CTCTACGTTA TCGACTCTTC
     7081 TGCGACGAGC ATCAGCCGTC GCAACATCGG ACATACCAGA TCCTACCTAC AAACGGCACG
     7141 AGCATCAGCC GTGCCGCCCA GCATCTTCAT CACTGTCCAA TCTTCAAGCG TGCACGATCA
     7201 CGGCACGCTC CACTGTCATT ACAGTCTTAT CCAATCAAAC TCCAGCAGCA TCAGCGCTGG
     7261 AGCTCCGACT ACATCAGGAC ATCCATCATT CAACGCTCGA CGATCACGCG AGCGTCCACA
     7321 GCATGCCTCA GACACTATCA TCAAACGGCT GCACGATCAC GGCAGCCGCT CCTACTGCCT
     7381 CTACGTTATC GACTCTTCTG CGACGAGCAT CAGCCGTCGC AACATCGGAC ATACCAGATC
     7441 CTACCTACAA ACGGCACGAG CATCAGCCGT GCCGCCCAGC ATCTTCATCA CTGTCCAATC
     7501 TTCAAGCGTG CACGATCACG GCACGCTCCA CTGTCATTAC AGTCTTATCC AATCAAACTC
     7561 CAGCAGCATC AGCGCTGGAG CTCCGACTAC ATCAGGACAT CCATCATTCA ACGCTCGACG
     7621 ATCACGCGAG CGTCCACAGC ATGCCTCAGA CACTATCATC AAACGGCTGC ACGATCACGG
     7681 CAGCCGCTCC TACTGCCTCT ACGTTATCGA CTCTTCTGCG ACGAGCATCA GCCGTCGCAA
     7741 CATCGGACAT ACCAGATCTT CAATcgATtt cgcgcgggat ccagaccacc tcccctgcga
     7801 gctaagctgg acagccaatg acgggtaaga gagtgacatt tttcactaac ctaagacagg
     7861 agggccgtca gagctactgc ctaatccaaa gacgggtaaa agtgataaaa atgtatcact
     7921 ccaacctaag acaggcgcag cttccgaggg atttgagatc cagacatgat aagatacatt
     7981 gatgagtttg gacaaaccaa aactagaatg cagtgaaaaa aatgccttat ttgtgaaatt
     8041 tgtgatgcta ttgccttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     8101 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     8161 taaaacctct acaaatgtgg tatggctgat tatgatctga agttcctata ctatttgaag
     8221 aataggaact tcggaatagg aacttcggat ctcatatggc catatggggg cgcctagaga
     8281 aggagtgagg gctggataaa gggaggatcg aggcggggtc gaacgaggag gttcaagggg
     8341 gagagacggg gcggatggag gaagaggagg cggaggctta gggtgtacaa agggcttgac
     8401 ccagggaggg gggtcaaaag ccaaggcttc ccaggtcacg atgtacccca cctggtctgg
     8461 gtgtccatgc gggccaggtg aaaagacctt gatcttaacc tgggtgatga ggtctcggtt
     8521 aaaggtgccg tctcgcggcc atccgacgtt aaaggttggc cattctgcag agcagaaggt
     8581 aacccaacgt ctcttcttga catctaccga ctggttgtga gcgatccgct cgacatcttt
     8641 ccagtgacct aaggtcaaac ttaagggagt ggtaacaggc tagcccgggc ccatattttc
     8701 agacaaatac agaaacacag tcagacagag acaacacaga acgatgctgc agcagacaag
     8761 acgcgcggcg cggcttcggt cccaaaccga aagcaaaaat tcagacggag gcgggaactg
     8821 ttttaggttc tcgtctccta ccagaaccac atatccctcc tctaaggggg gtgcaccaaa
     8881 gagtcctaaa cgatcgggat tttaggactc aggtcgggcc acaaaaacgg cccccgaagt
     8941 ccctgggacg tctcccaggg ttgcggccgg gtgttccgaa ctcgtcagtt ccaccacggg
     9001 tccgccagat acagagctag ttagctaact agtaccgacg caggcgcata aaatcagtca
     9061 tagacactag acaatcggac agacacagat aagttgctgg ccagcttacc tcccggtggt
     9121 gggtcggtgg tccctgggca ggggtctccc gatcccggac gagcccccaa atgaaagacc
     9181 cccgctgacg ggtagtcaat cactcagagg agaccctccc aaggaacagc gagaccacaa
     9241 gtcggatgca actgcaagag ggtttattgg atacacgggt acccgggcga ctcagtcaat
     9301 cggaggactg gcgccccgag tgaggggttg tgggctcttt tattgagctc ggggagcata
     9361 agcgcgcgaa cagaagcgag aagcgaactg attggttagt tcaaataagg cacagggtca
     9421 tttcaggtcc ttggggcacc ctggaaacat ctgatggttc tctagaaact gctgagggct
     9481 ggaccgcatc tggggaccat ctgttcttgg ccctgagccg gggcaggaac tgcttaccac
     9541 agatatcctg tttggcccat attcagctgt tccatctgtt cttggccctg agccggggca
     9601 ggaactgctt accacagata tcctgtttgg cccatattca gctgttccat ctgttcctga
     9661 ccttgatctg aacttttcta ttctcagtta tgtatttttc catgccttgc aaaatggcgt
     9721 tacttaagct agcttgccaa acctacaggt ggggtctttc attcacatgt atatgtcaaa
     9781 aataaaaatc aactaattga ctagtaatta atatgactgg cataatggga aattgatcct
     9841 gacagatgca aactggcttg ctcagcagcg catttatgtt gtcaactgag gaaggaaacg
     9901 ttaatgacag aaactctaag taatttccac gtttatctat ttttatttat actagctttg
     9961 gtaacaggaa tattgcagca ttcatgcaca ttgaaaccct tatgaaataa aaacatctgt
    10021 gcatttaaaa tggaattaac attttaaatg ttaaaaaaag ctggcttagc ttccccccgc
    10081 cccctagggc atagaacaag tcaaatgctt tatatatttg agtttgggat gtattaggaa
    10141 actcctaaga gcaaagctag cttg
//