LOCUS       DIO_GluCl_Alpha         7104 bp ds-DNA     circular     17-JAN-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: dio_glucl_alpha.gb
FEATURES             Location/Qualifiers
     rep_origin      complement(182..770)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(941..1732)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(1733..1801)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(1802..1906)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(1932..2387)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2460..2588
                     /label="ITR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2649..2898
                     /label="hCMV I/E Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2649..3586
                     /label="shortCAG promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        2899..3176
                     /label="chicken β-actin promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3177..3402
                     /label="CBA intron"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3403..3533
                     /label="rbG 1 intron"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3534..3586
                     /label="rabHBb 2 exon"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    3617..3650
                     /label="lox2272"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    3701..3734
                     /label="loxP"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     misc_feature    complement(3743..5851)
                     /label="GluCl_Alpha"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(3896..4612)
                     /label="Cerulean"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     protein_bind    complement(5864..5897)
                     /label="lox2272"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    complement(5948..5981)
                     /label="loxP"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     misc_feature    6006..6594
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6477..6488)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     polyA_signal    6637..6758
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    6920..7048
                     /label="ITR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagagtacc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2701 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2761 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2821 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2881 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     2941 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     3001 gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
     3061 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
     3121 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
     3181 tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg cccgcccgcc
     3241 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     3301 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
     3361 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggc tgtccgcggg
     3421 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     3481 gcggctctAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGctcctgg
     3541 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattggat cgggatcctc
     3601 tagagtcgac tccggaataa cttcgtatag gatactttat acgaagttat gcagaatggt
     3661 agctggattg tagctgctat tagcaatatg aaacctctta ataacttcgt atagcataca
     3721 ttatacgaag ttatGGcgcg cctcagaaca gaacgttctg ctggccgaag cggctccagt
     3781 acaggatgtt gaacacgaag aacagcacgg ggaacagggc gcggctgatc aggtccacgc
     3841 gcttgctgat gtcgttccac tcggtcttgg tctccagcag gttcaggaag gaattCTTGT
     3901 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT
     3961 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GCTTGGACTG GGTGCTCAGG TAGTGGTTGT
     4021 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT
     4081 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT
     4141 GCTTGTCGGC GGTGATATAG ACGTTGTCGC TGATGGCGTT GTACTCCAGC TTGTGCCCCA
     4201 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT
     4261 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG
     4321 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT
     4381 GGTCGGGGTA GCGGGCGAAG CACTGCACGC CCCAGGTCAG GGTGGTCACG AGGGTGGGCC
     4441 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG
     4501 CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT
     4561 CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATggaattca
     4621 tctgccggcg gttcaggggc acggtgcgga ctttctcctc ctggttgaac accttggtgg
     4681 ggacgtcgtt gtgcaggttc tgcagcaggg ggatctcggc cttctcgcgc tcggtgcggg
     4741 ccttgcgctc cacgccctgc ttgttcgcga tgtggttaac cagggcgaac tccagcaggg
     4801 cgcagaagat gaaggtcatg caggcgccga tccacacgtc gatggccttg atgtagctca
     4861 cggggggcag ctggctgttg atgccagcgg actgagcggt catggtgaac agggtggtca
     4921 cgcccagggt gacgcgggcg gggatggcgg tgcggtcgaa ccagaaggac acccaggaca
     4981 cgatcacgag catgcagctg gggatgtaca gttgcagcag gtagaaggag aactcgcgct
     5041 tcagctggat ggtggtcctc aggcagctgt agatgccggt gttggtgacg ctggtgcagt
     5101 aggtggtgct ggtgttggtc agctggaagc tgggcaggct ggaggacagg ccgaccttca
     5161 gctgcagggg gctgtgctcc ttccacagat actcgatgtc cttggtggtg taggcgtagg
     5221 aggccaggtc gatgctgcac tgctgcacgt ccatcgggta gtactgcagg tacatggggc
     5281 agctcagcac caggctgatg cggacggagt acaggacggt gccgtcgttg tggatgcgga
     5341 tcaggacgtt gggcttgtcg atggtgtgct tgtaagcctg cttctcgttg gggaagaagg
     5401 tgtcgggcat ccagatctgg tggcccacgg tcaggatgac gaagtcgggc tggccgtcgc
     5461 ccttgacgcc gtaggacagg cgcttgtcga tccagctctc gcgcagggtc agctgggcgg
     5521 agtactccat gttcaccacg tcgatcttgc tgatggtgcg cagcagcatg ttcacgctca
     5581 ccaccacggg gccgccgttg tcggtggggg ggcgcacgcg gaagtcgtag ccgctggtga
     5641 acaggtgggc caggatcttg ctgtcggagg tctggggctg ctcgatgggg atgtggatgg
     5701 gggaggtcag gcgagccagg gactccaggg tggtagtgcc gttgtcgttg tcgtcctcga
     5761 agatgtcctg gctcttggtg cgggcctgct gggcctggat ccccaggatc agggaagcga
     5821 tgatcagctt gcccacgatc cacgtggcca tggtggggct agcataactt cgtataaagt
     5881 atcctatacg aagttatttg ccttaaccca gaaattatca ctgttattct ttagaatggt
     5941 gcaaagaata acttcgtata atgtatgcta tacgaagtta tgaattcgat atcaagctta
     6001 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6061 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6121 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6181 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6241 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6301 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6361 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     6421 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6481 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6541 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     6601 accgtcgacc cgggcggccg cttcgagcag acatgataag atacattgat gagtttggac
     6661 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     6721 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     6781 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca
     6841 aatgtggtaa aatcgataag gatcttccta gagcatggct acgtagataa gtagcatggc
     6901 gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     6961 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     7021 gcggcctcag tgagcgagcg agcgcgcagc tgcattaatg aatcggccaa cgcgcgggga
     7081 gaggcggttt gcgtattggg cgct
//