LOCUS       Exported                9960 bp ds-DNA     circular SYN 20-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 17, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9960
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        396..681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        683..960
                     /label=chicken beta-actin promoter
     intron          961..1189
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1201..1210
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3109..3129
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4429..4476
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4480..4506
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4486..4512
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4525..4545
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4552..4572
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4612..5307
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             5353..5538
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     polyA_signal    5572..5779
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5780..6020
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     enhancer        6649..6952
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6953..7156
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             7185..7895
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    7912..8136
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8163..8267
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8268..9128
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9299..9887
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tatcccctga ttctctcgag ttaaccctag aaagataatc atattgtgac gtacgttaaa
       61 gataatcatg cgtaaaattg acgcatgtgt tttatcgatc tgtatatcga ggtttattta
      121 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
      181 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt
      241 ctataaagta acaaaacttt taaacattct ctcttttaca aaaataaact tattttgtac
      301 tttaaaaaca gtcatgttgt attataaaat aagtaattag cttaacttat acataataga
      361 aacaaattat acttattaat taatctagag gtacccgtta cataacttac ggtaaatggc
      421 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg
      481 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      541 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      601 tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      661 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      721 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      781 atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      841 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      901 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      961 ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     1021 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     1081 tccgggctgt aattagctga gcaagaggta agggtttaag ggatggttgg ttggtggggt
     1141 attaatgttt aattacctgg agcacctgcc tgaaatcact ttttttcagg ttggaccggt
     1201 gccaccatgg acatgaacca tgaccaggaa tttgaccccc caaaggttta cccacctgtg
     1261 ccagctgaga agaggaagcc catccgcgtg ctgtctctct ttgatgggat tgctacaggg
     1321 ctcctggtgc tgaaggacct gggcatccaa gtggaccgct acattgcctc cgaggtgtgt
     1381 gaggactcca tcacggtggg catggtgcgg caccagggaa agatcatgta cgtcggggac
     1441 gtccgcagcg tcacacagaa gcatatccag gagtggggcc cattcgacct ggtgattgga
     1501 ggcagtccct gcaatgacct ctccattgtc aaccctgccc gcaagggact ttatgagggt
     1561 actggccgcc tcttctttga gttctaccgc ctcctgcatg atgcgcggcc caaggaggga
     1621 gatgatcgcc ccttcttctg gctctttgag aatgtggtgg ccatgggcgt tagtgacaag
     1681 agggacatct cgcgatttct tgagtctaac cccgtgatga ttgacgccaa agaagtgtct
     1741 gctgcacaca gggcccgtta cttctggggt aaccttcctg gcatgaacag gcctttggca
     1801 tccactgtga atgataagct ggagctgcaa gagtgtctgg agcacggcag aatagccaag
     1861 ttcagcaaag tgaggaccat taccaccagg tcaaactcta taaagcaggg caaagaccag
     1921 catttccccg ttttcatgaa cgagaaggag gacatcctgt ggtgcactga aatggaaagg
     1981 gtgtttggct tccccgtcca ctacacagac gtctccaaca tgagccgctt ggcgaggcag
     2041 agactgctgg gccgatcgtg gagcgtgccg gtcatccgcc acctcttcgc tccgctgaag
     2101 gaatattttg cttgtgtgtc tagcggcaat agtaacgcta acagccgcgg gccgagcttc
     2161 agcagcggcc tggtgccgtt aagcttgcgc ggcagccata tgggccctat ggagatatac
     2221 aagacagtgt ctgcatggaa gagacagcca gtgcgggtac tgagcctctt cagaaacatc
     2281 gacaaggtac taaagagttt gggcttcttg gaaagcggtt ctggttctgg gggaggaacg
     2341 ctgaagtacg tggaagatgt cacaaatgtc gtgaggagag acgtggagaa atggggcccc
     2401 tttgacctgg tgtacggctc gacgcagccc ctaggcagct cttgtgatcg ctgtcccggc
     2461 tggtacatgt tccagttcca ccggatcctg cagtatgcgc tgcctcgcca ggagagtcag
     2521 cggcccttct tctggatatt catggacaat ctgctgctga ctgaggatga ccaagagaca
     2581 actacccgct tccttcagac agaggctgtg accctccagg atgtccgtgg cagagactac
     2641 cagaatgcta tgcgggtgtg gagcaacatt ccagggctga agagcaagca tgcgcccctg
     2701 accccaaagg aagaagagta tctgcaagcc caagtcagaa gcaggagcaa gctggacgcc
     2761 ccgaaagttg acctcctggt gaagaactgc cttctcccgc tgagagagta cttcaagtat
     2821 ttttctcaaa actcacttcc tcttggaggg ccgagctctg gcgcaccccc accaagtgga
     2881 ggatctcctg ccgggtcccc aacatctact gaagaaggca ccagcgaatc cgcaacgccc
     2941 gagtcaggcc ctggtacctc cacagaacca tctgaaggta gtgcgcctgg ttccccagct
     3001 ggaagcccta cttccaccga agaaggcacg tcaaccgaac caagtgaagg atctgcccct
     3061 gggaccagca ctgaaccatc tgagcgagga agcggaagcg gatccatgcc taagaagaag
     3121 agaaaggtga tgggaaaggg cgtgctggcc aaggtgatga aatacgagct gagatacctg
     3181 gatggttgtg gcgacttcag caatatgcag gagcaggtgt gggccctgca gcggcagaca
     3241 cgggaaatcc tgaacagatc catccaaatc gccttccaat ggcgctgcgc caacagcgag
     3301 caccacagaa agaccggcga gtacctggac ctgaaaacgg aaaccggcta caagagactg
     3361 gatggccaca tctacaactg cctgaagggc cagtacgagg acatggccac atctaacctg
     3421 aacgccacca tccagaaggc ttggaagaag tataactcca gcaagaagga aatcctgagg
     3481 ggcagcatga gcatccccag ctacaagatg aaccagcctc tgcgactgga caagaatacc
     3541 gtgaaactgt ctgagggcga gcggaaccca atcgtgaccc tgacactgtt tagcgacaag
     3601 ttcaagcggg cccagggcgt gtccaacgtg aagtttagca tgcctctgca cgacggcacc
     3661 cagagagcca tcttcgccaa cctgatgaac ggcacctacc agctgggaga gtgccagctg
     3721 gtgtacaaac ggcctaagtg gttcctgttc gtgacataca agttcccccc cgtggaacat
     3781 cctctcgatc ctgacaagat tctgggcgtc gccatgggcg aggcctgcgc gctttatgcc
     3841 tctacattcg gcgagcacgg ctacctgaag atcgatggag gcgagattac aaagtacgcc
     3901 aagaagatgg aagctagaat ccggagcatg cagaagcagg ctgctcactg tggcgaaggc
     3961 agaatcgggc acggcaccaa aacaagagtg tctgtggtgt accaggccaa ggacaaggtg
     4021 gccagattca gagataccat caaccacaga tactctaagg ccctgatcga ctacgccctg
     4081 aagaaccagt gtggcaccat ccagatggaa gatctgaccg gcatcaagga agatacagga
     4141 tttccaaagt tcctgagaca ttggacctac tacgacctgc agagcaagat cgaggctaag
     4201 gccgccgagc acggcatcca agttgtcaag atcaacccta gacacaccag ccagcgctgc
     4261 agcagatgtg gacacatcga caaagccaat agaaccagcc aagctgattt ctgctgcacc
     4321 aagtgcggct tcagcgccaa tgccgccttt aatgccagcc agaacatcag catcagaaac
     4381 atcgacaaga ttatcgccaa ggctatcggc gccaaccgga agcagaccaa aaggccggcg
     4441 gccacgaaga aggccggcca ggcaaagaag aagaaggcct atccctatga cgtgcccgat
     4501 tatgccagcc tgggcagcgg ctcccccaag aaaaaacgca aggtggaaga tcctaagaaa
     4561 aagcggaaag tggacggcat tggtagtggg agcaacggca gcagcggatc cagcgagctg
     4621 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
     4681 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     4741 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
     4801 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
     4861 cctgagggct tcacatggga gagagtcacc acatacgagg acgggggcgt gctgaccgct
     4921 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     4981 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
     5041 gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
     5101 gtgggcggga gccatctgat cgcaaacatc aagaccacat atagatccaa gaaacccgct
     5161 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag
     5221 gccaacaacg aaacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
     5281 cctagcaaac tggggcacaa gcttaatggc ggtggcggag ggatggatgc taagtcacta
     5341 actgcctggt cccggacact ggtgaccttc aaggatgtat ttgtggactt caccagggag
     5401 gagtggaagc tgctggacac tgctcagcag atcgtgtaca gaaatgtgat gctggagaac
     5461 tataagaacc tggtttcctt gggttatcag cttactaagc cagatgtgat cctccggttg
     5521 gagaagggag aagagcccta ggaattccta gagctcgctg atcagcctcg actgtgcctt
     5581 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5641 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5701 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga
     5761 atagcaggca tgctggggag agggcctatt tcccatgatt ccttcatatt tgcatatacg
     5821 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
     5881 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     5941 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     6001 atatatcttg tggaaaggac gaaacaccga gggccgactt cccggcccaa aatcgagaca
     6061 gtagccgtaa aacgttgagt ttcagcgtgg gcgacacact cgaaaaggtt aagatatgca
     6121 catagtaatc cgtgcatgag ccgcgaaagc ggcttgaagg ttgtgcagtg ccaggtgaaa
     6181 ttttattttt ttgcggccgc gtcgacggcg cgccctagac gaagcagctc cagcctacac
     6241 aatcgctcaa gacgtgtaat gcttttatta tatattagtc acgatatcta taacaagaaa
     6301 atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata attatcgtat
     6361 gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact cacgcggtcg
     6421 ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg ggagctccaa
     6481 gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag
     6541 caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaaggcaa
     6601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctctagacg ttacataact
     6661 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     6721 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     6781 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     6841 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     6901 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     6961 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     7021 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     7081 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     7141 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcatggtg agcaagggcg
     7201 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     7261 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     7321 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     7381 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     7441 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgatg
     7501 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     7561 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaaaacca
     7621 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     7681 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     7741 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     7801 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     7861 actccaccgg cggcatggac gagctgtaca agtaactctt ggcccggatc cctgtgcctt
     7921 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     7981 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     8041 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaaaaca
     8101 atagcaggca tgctggggat gcggtgggct ctatggggat ccgcactttt cggggaaatg
     8161 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8221 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8281 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8341 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8401 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8461 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8521 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8581 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8641 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8701 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8761 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8821 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8881 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8941 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9001 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9061 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9121 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9181 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9241 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9301 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9361 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9421 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9481 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9541 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9601 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9661 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9721 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9781 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9841 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9901 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
//
